Methods for generating target specific probes for solution based capture

ABSTRACT

Provided herein are compositions and kits for single-stranded nucleic acid probes, and methods for making the single-stranded nucleic acid probes, where the single-stranded nucleic acid probes comprise a probe region having a predetermined sequence which is flanked by a 5′ region having a first restriction enzyme recognition sequence and flanked by a 3′ region having a second restriction enzyme recognition sequence, and a region which hybridizes to a capture nucleic acid molecule. The single-stranded nucleic acid probes are useful for solution-based capture methods.

This application claims the filing date benefit of U.S. Provisional Application Nos.: 61/164,859, filed on Mar. 30, 2009. The contents of each foregoing patent applications are incorporated by reference in their entirety.

Throughout this application various publications are referenced. The disclosures of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains.

FIELD

The present invention relates generally to the field of genomic analysis, and more particularly, to methods and kits for making libraries of single-stranded nucleic acid probes comprising predetermined sequences.

BACKGROUND

The ability to sequence deoxyribonucleic acid (DNA) accurately and rapidly is revolutionizing biology and medicine. The pharmacogenomics challenge is to comprehensively identify the genes and functional polymorphisms associated with the variability in drug response. Screens for numerous genetic markers performed for populations large enough to yield statistically significant data are needed before associations can be made between a given genotype and a particular disease.

The study of complex genomes, and in particular, the search for the genetic basis of disease in humans, requires genotyping on a massive scale, which is demanding in terms of cost, time, and labor. Such costly demands are even greater when the methodology employed involves serial analysis of individual DNA samples, i.e., separate reactions for individual samples. Resequencing of polymorphic areas in the genome that are linked to disease development will contribute greatly to the understanding of diseases, such as cancer, and therapeutic development. Oligonucleotide libraries are the cornerstone of sequence-based gene resequencing and digital profiling strategies. To realize the full commercial potential of various high-throughput sequencing platforms, the cost of generating oligonucleotide libraries must be reduced by a substantial amount. Thus, there is a need for cost-effective methods for preparing populations of high quality oligonucleotide probes with sufficient yield for use in high throughput sequencing platforms and solution based capture methods.

SUMMARY

Provided herein are methods for generating a population of single-stranded nucleic acid probes, each probe comprising a predetermined nucleotide sequence, the method comprising: (a) providing a starting population of linear double-stranded nucleic acid precursor molecules each precursor molecule having (i) a probe region having the predetermined sequence which is flanked at a 5′ and a 3′ end by a first and a second restriction enzyme recognition sequence for generating ligation substrates and for ligating a plurality of the double-stranded nucleic acid precursor molecules into head-to-tail concatemers (ii) the 5′ flanking region including the first restriction enzyme recognition sequence and (iii) the 3′ flanking region including the second restriction enzyme recognition sequence; (b) contacting the 5′ and 3′ flanking regions of the linear double-stranded nucleic acid precursor molecules with the first and second restriction enzymes to cleave the first and second restriction enzyme recognition sequences so as to generate the ligation substrates; (c) ligating the ligation substrates together so as to generate a plurality of random head-to-tail concatemers; (d) amplifying the plurality of head-to-tail concatemers; (e) contacting the amplified head-to-tail concatemers with the first and second restriction enzymes so as to release a plurality double-stranded monomer linear precursor molecules; and (f) selectively removing one strand of the double-stranded monomer linear precursor molecules so as to generate a population of single-stranded nucleic acid probes, each probe comprising the predetermined nucleotide sequence.

In one embodiment, the single-stranded nucleic acid probes further comprise a region which hybridizes to a capture nucleic acid molecule.

In yet another embodiment, the selectively removing one strand from the double-stranded monomer linear precursor molecules comprises: (a) contacting the released precursor molecules of step (e) above with alkaline phosphatase; (b) contacting the released precursor molecules of step (e) above with a third restriction enzyme which cleaves the third restriction enzyme recognition sequence; and (c) contacting the released precursor molecules of step (e) above with an exonuclease so as to selectively degrade the one strand of the double-stranded monomer linear precursor molecules.

In one embodiment, the exonuclease is lambda exonuclease.

In another embodiment, the members of the starting population of the linear double-stranded nucleic acid precursor molecules each comprise the same nucleotide sequence in the 5′ flanking region or each comprise the same nucleotide sequence in the 3′ flanking region.

In another embodiment, the 3′ flanking region further comprises a third restriction enzyme recognition sequence.

In another embodiment, the members of the starting population of the linear double-stranded nucleic acid precursor molecules each comprise the same predetermined sequences or different predetermined sequences.

In another embodiment, the ligation substrates of step (b) comprise overhanging nucleic acid ends capable of annealing together.

In another embodiment, the first or second restriction enzyme recognition sequence is cleaved by a type II restriction enzyme.

In another embodiment, the first or second restriction enzyme recognition sequence is cleaved by a Bsm1 enzyme.

In another embodiment, each predetermined nucleotide sequence in the population of linear double-stranded nucleic acid precursor molecules comprise a nucleotide sequence which is at least 95% identical to at least a portion of a sense or anti-sense strand of a target nucleic acid sequence.

In another embodiment, the predetermined sequence hybridizes to one target sequence or hybridizes to different target sequences.

In another embodiment, the predetermined sequences in the population of linear double-stranded nucleic acid precursor molecules hybridize to at least 10 different exon nucleotide sequences.

In another embodiment, the predetermined sequences in the population of linear double-stranded nucleic acid precursor molecules hybridize to at least 1000 different exon nucleotide sequences.

In another embodiment, the predetermined sequences hybridize to the target sequence at an interval of at least every 35 bases across the target sequence.

In another embodiment, the predetermined sequences hybridize to the target sequence of interest at an interval of one base across the target sequence.

In another embodiment, the probe region comprises 20-200 nucleotides.

In another embodiment, the predetermined nucleotide sequence comprises 10-50 nucleotides.

In another embodiment, the region of the single-stranded nucleic acid probe which hybridizes to the capture nucleic acid molecule comprises 10-50 nucleotides.

In another embodiment, the amplifying according to step (d) comprises isothermal amplification.

In another embodiment, the amplifying according to step (d) comprises random amplification primers.

In another embodiment, the random amplification primers each comprise a random 7-mer oligonucleotide and two additional nitroindole residues at the 5′ end.

In another embodiment, the random amplification primers each comprise a random 7-mer oligonucleotide and a phosphorothioate linkage to the 3′ end.

In another embodiment, the capture nucleic acid molecule further comprises a protein binding partner.

In another embodiment, the protein binding partner is biotin.

In another embodiment, each single-stranded nucleic acid probe comprises (i) the predetermined nucleotide sequence having a nucleotide sequence which is at least 95% identical to at least a portion of a sense or an anti-sense strand of a target nucleic acid sequence and (ii) a region which hybridizes to a capture nucleic acid molecule.

Provided herein are also a population of single-stranded nucleic acid probes generated by the disclosed methods.

Provided herein are also methods, wherein the starting population of linear double-stranded nucleic acid precursor molecules is generated by steps comprising: (a) providing a population of a first single-stranded nucleic acid molecule comprising the 5′ flanking region, the probe region which comprises the predetermined sequence, and the capture sequence; (b) providing a population of a second single-stranded nucleic acid molecules comprising the sequence which is complementary to the capture sequence, and the 3′ flanking region; (c) annealing the first and second populations of the single-stranded nucleic acid molecules to form a nucleic acid duplex having overhanging 5′ ends; and (d) conducting a polymerase-dependent strand extension reaction on the overhanging 5′ ends so as to generate the population of double-stranded nucleic acid precursor molecules.

Provided herein are also, methods for enriching a target nucleic acid sequence of interest from a nucleic acid library, comprising: (a) contacting the population of single-stranded nucleic acid probes of the method above with the nucleic acid library having at least one target nucleic acid sequence of interest to form a mixture having unhybridized nucleic acid sequences and duplexes, each duplex having the single-stranded nucleic acid probe hybridized to the target nucleic acid sequence of interest; (b) contacting the duplexes with a population of capture nucleic acid molecules to form complexes having the single-stranded nucleic acid probe hybridized to the target nucleic acid sequence of interest and hybridized to the capture nucleic acid molecule; (c) separating the complex from the mixture; and (d) eluting the target nucleic acid sequence of interest from the complex.

DESCRIPTION OF THE DRAWINGS

FIG. 1A illustrates a population of target capture probes (200) for solution-based capture, designed to provide low density coverage of a target exon (10), as described in Example 1;

FIG. 1B illustrates a population of target capture probes (200) for solution-based capture, designed to provide high density coverage of a target exon (10), as described in Example 1;

FIG. 1C is a schematic diagram of a representative target capture probe (200) comprising a target-specific binding region (202) bound to a nucleic acid target (10), and a region for binding to a capture reagent (204) bound to a universal adaptor oligonucleotide (300) comprising a moiety (310) that binds to a capture reagent;

FIG. 2 illustrates a method of enriching a population of DNA molecules for target regions of interest using capture probes (200) generated in accordance with an embodiment of the present invention;

FIG. 3 is a flow chart of the steps of a method for generating a population of capture probes in accordance with various embodiments of the present invention;

FIG. 4 illustrates a method of generating a population of single-stranded capture probes (200) in accordance with an embodiment of the present invention;

FIG. 5 illustrates more detail of the method illustrated in FIG. 4; and

FIG. 6 graphically illustrates the fold enrichment obtained using capture probes generated in accordance with the methods of the present invention, as described in Example 2.

DETAILED DESCRIPTION

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art to which these inventions belong. All patents, patent applications, published applications, treatises and other publications referred to herein, both supra and infra, are incorporated by reference in their entirety. If a definition and/or description is explicitly or implicitly set forth herein that is contrary to or otherwise inconsistent with any definition set forth in the patents, patent applications, published applications, and other publications that are herein incorporated by reference, the definition and/or description set forth herein prevails over the definition that is incorporated by reference.

The practice of the present disclosure will employ, unless otherwise indicated, conventional techniques of molecular biology, microbiology and recombinant DNA techniques, which are within the skill of the art. Such techniques are explained fully in the literature. See, for example, Sambrook, J., and Russell, D. W., 2001, Molecular Cloning: A Laboratory Manual, Third Edition; Ausubel, F. M., et al., eds., 2002, Short Protocols in Molecular Biology, Fifth Edition.

As used herein, the terms “comprising” (and any form or variant of comprising, such as “comprise” and “comprises”), “having” (and any form or variant of having, such as “have” and “has”), “including” (and any form or variant of including, such as “includes” and “include”), or “containing” (and any form or variant of containing, such as “contains” and “contain”), are inclusive or open-ended and do not exclude additional, unrecited additives, components, integers, elements or method steps.

As used herein, the terms “a,” “an,” and “the” and similar referents used herein are to be construed to cover both the singular and the plural unless their usage in context indicates otherwise. Accordingly, the use of the word “a” or “an” when used in the claims or specification, including when used in conjunction with the term “comprising”, may mean “one,” but it is also consistent with the meaning of “one or more,” “at least one,” and “one or more than one.”

As used herein, the term “nucleic acid molecule” and its variants encompasses both deoxyribonucleotides and ribonucleotides and refers to a polymeric form of nucleotides including two or more nucleotide monomers. The nucleotides can be naturally occurring, artificial, and/or modified nucleotides.

As used herein, an “isolated nucleic acid” and its variants is a nucleic acid molecule that exists in a physical form that is non-identical to any nucleic acid molecule of identical sequence as found in nature; “isolated” does not require, although it does not prohibit, that the nucleic acid so described has itself been physically removed from its native environment. For example, a nucleic acid can be said to be “isolated” when it includes nucleotides and/or internucleoside bonds not found in nature. When, instead, composed of natural nucleosides in phosphodiester linkage, a nucleic acid can be said to be “isolated” when it exists at a purity not found in nature, where purity can be adjudged with respect to the presence of nucleic acids of other sequences, with respect to the presence of proteins, with respect to the presence of lipids, or with respect to the presence of any other component of a biological cell, or when the nucleic acid lacks a sequence that flanks an otherwise identical sequence in an organism's genome, or when the nucleic acid possesses a sequence not identically present in nature. As so defined, “isolated nucleic acid” includes nucleic acids integrated into a host cell chromosome at a heterologous site, recombinant fusions of a native fragment to a heterologous sequence, recombinant vectors present as episomes, or as integrated into a host cell chromosome.

As used herein, “subject” and its variants refers to an organism or to a cell sample, tissue sample, or organ sample derived therefrom, including, for example, cultured cell lines, biopsy, blood sample, or fluid sample containing a cell. For example, an organism may be an animal, including but not limited to, an animal such as a cow, a pig, a mouse, a rat, a chicken, a cat, a dog, etc., and is usually a mammal, such as a human.

As used herein, the term “specifically bind” and its variants refers to two components (e.g., target-specific binding region and target) that are bound (e.g., hybridized, annealed, complexed) to one another sufficiently that the intended capture and enrichment steps can be conducted. As used herein, the term “specific” refers to the selective binding of two components (e.g., target-specific binding region and target) and not generally to other components unintended for binding to the subject components.

As used herein, the term “high stringency hybridization conditions” and its variants means any condition in which hybridization will occur when there is at least 95%, preferably about 97% to 100% nucleotide complementarity (identity) between the nucleic acid sequences of the nucleic acid molecule and its binding partner. However, depending upon the desired purpose, the hybridization conditions may be “medium stringency hybridization,” which can be selected that require less complementarity, such as from about 50% to about 90% (e.g., 60%, 70%, 80%, 85%). The comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm of Karlin and Altschul (Proc. Natl. Acad. Sci. USA 87:2264-2268 (1990)), modified as in Karlin and Altschul (Proc. Natl. Acad. Sci. USA 90:5873-5877 (1993)). Such an algorithm is incorporated into the NBLAST and XBLAST programs of Altschul et al. (J. Mol. Biol. 215:403-410 (1990)).

As used herein, the term “complementary” and its variants refers to nucleic acid sequences that are capable of base-pairing according to the standard Watson-Crick complementary rules. That is, the larger purines will base pair with the smaller pyrimidines to form combinations of guanine paired with cytosine (G:C) and adenine paired with either thymine (A:T) in the case of DNA, or adenine paired with uracil (A:U) in the case of RNA.

As used herein, the term “target” and its variants refers to a nucleic acid molecule or polynucleotide whose presence and/or amount and/or sequence is desired to be determined and which has an affinity for a given target capture probe. Examples of targets include regions of genomic DNA, PCR amplified products derived from RNA or DNA, DNA derived from RNA or DNA, ESTs, cDNA, and mutations, variants or modifications thereof.

As used herein, the term “predetermined nucleic acid sequence” and its variants means that the nucleic acid sequence of a nucleic acid probe is known and was chosen before synthesis of the nucleic acid molecule in accordance with the invention disclosed herein.

As used herein, the term “essentially identical” and its variants as applied to synthesized and/or amplified nucleic acid molecules refers to nucleic acid molecules that are designed to have identical nucleic acid sequences, but that may occasionally contain minor sequence variations in comparison to a desired sequence due to base changes introduced during the nucleic acid molecule synthesis process, amplification process, or due to other processes in the method. As used herein, essentially identical nucleic acid molecules are at least 95% identical to the desired sequence, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99% identical, or absolutely identical, to the desired sequence.

As used herein, the term “resequencing” and its variants refers to a technique that determines the sequence of a genome of an organism using a reference sequence that has already been determined. It should be understood that resequencing may be performed on both the entire genome/transcriptome of an organism or a portion of the genome/transcriptome large enough to include the genetic change of the organism as a result of selection. Resequencing may be carried out using various sequencing methods, such as any sequencing platform amenable to producing DNA sequencing reads that can be aligned back to a reference genome, and is typically based on highly parallel technologies such as, for example, dideoxy “Sanger” sequencing, pyrosequencing on beads (e.g., as described in U.S. Pat. No. 7,211,390, assigned to 454 Life Sciences Corporation, Branford, Conn.), ligation based sequencing on beads (e.g., Applied Biosystems Inc,/Invitrogen), sequencing on glass slides (e.g., Illumina Genome Analyzer System, based on technology described in WO 98/44151 (Mayer, P., and Farinelli, L.), microarrays, or fluorescently labeled micro-beads.

As used herein, the term “target nucleotide” and its variants refers to a nucleic acid molecule or polynucleotide in a starting population of nucleic acid molecules having a target sequence whose presence and/or amount and/or nucleotide sequence is desired to be determined and which has an affinity for a given target capture probe.

As used herein, the term “target sequence” and its variants refers generally to a nucleic acid sequence on a single strand of nucleic acid. The target sequence may be a portion of a gene, a regulatory sequence, genomic DNA, cDNA, RNA including mRNA and rRNA, or others. The target sequence may be a target sequence from a sample, or a secondary target such as a product of an amplification reaction.

As used herein, the term “processing” and its variants refers generally to a manipulation of a precursor nucleic acid substrate into a processed form of the substrate, such as by cleavage with a restriction endonuclease, modification and/or amplification with DNA polymerases, manipulation of DNA termini (e.g., by adding terminal 5′ phosphates with a polynucleotide kinase or removing 5′ terminal phosphates with a suitable phosphatase), degradation of unwanted DNA strands with exonuclease, and the like.

As used herein, the term “head to tail concatemer” and its variants refers to at least two or more monomeric structures each having a first end and a second end, such as double-stranded nucleic acid molecules, covalently joined in the configuration of the second end of the first monomer joined to the first end of the second monomer.

Other objects, features and advantages of the disclosed compositions, methods, systems and kits will become apparent from the following detailed description. It should be understood, however, that the detailed description and the specific examples, while indicating specific embodiments, are given by way of illustration only, since various changes and modifications within the spirit and scope of the inventions provided herein will become apparent to those skilled in the art from this detailed description.

Provided herein are compositions, systems, methods and kits for generating a population of single-stranded nucleic acid probes, each probe comprising a predetermined nucleic acid sequence. The methods of the invention are useful in any situation in which it is desirable to make populations of single-stranded nucleic acid molecules (hundreds to thousands, to tens of thousands, to hundreds of thousands, to millions of oligonucleotides), wherein each nucleic acid molecule has a predetermined nucleic acid sequence. For example, the methods may be used to generate a high quantity of a complex population (e.g., library) of single-stranded nucleic acid probes, while maintaining a uniform representation of the individual nucleic acid probes within the population. The applications for pools of oligonucleotides include, but are not limited to, using the oligonucleotides to generate primers for PCR amplification, primers for multiplexing PCR and transcription, probes for SNP (single nucleotide polymorphism) detection, and libraries of nucleic acid probes for genomic analysis, RNA expression analysis, including siRNA and shRNA expression analysis. In some embodiments, the methods according to this aspect of the invention are used to generate a library of target specific probes for solution based capture methods, as described in Examples 1-3 herein.

The methods according to this aspect of the invention comprise (a) providing a starting population of double-stranded nucleic acid precursor molecules, wherein each precursor molecule in the starting population comprises a probe region comprising a predetermined sequence that is flanked on the 5′ end by a 5′ flanking region comprising a first processing site and is flanked on the 3′ end by a 3′ flanking region comprising a second processing site, wherein the first and second processing sites are selected to generate ligation substrates for ligation of a plurality of the double-stranded nucleic acid precursor molecules into head-to-tail concatemers; (b) processing the 5′ and 3′ flanking regions of the double-stranded nucleic acid precursor molecules to generate ligation substrates; (c) ligating the ligation substrates together to generate head-to-tail concatemers; (d) amplifying the head-to-tail concatemers; (e) processing the amplified head-to-tail concatemers to release double-stranded monomer precursor molecules; and (f) selectively removing the complement strand of the double-stranded monomer precursor molecules to generate a population of single-stranded nucleic acid probes each probe a predetermined nucleic acid sequence.

In one embodiment of the method, a population of target specific capture probes (e.g., a library of capture probes) is generated that may be used in solution based capture methods for enriching a population of DNA molecules for one or more target sequences of interest, such as for resequencing analysis. In accordance with this embodiment, each single-stranded capture probe oligonucleotide in the population of capture probes comprises (i) a target-specific binding region consisting of a nucleic acid sequence that is at least 95% identical to at least a portion of the sense or antisense strand of a target nucleic acid sequence of interest, and (ii) a region for binding to a capture reagent. The methods according to this embodiment of the invention can be used to create populations of single-stranded capture nucleic acid molecules (i.e., capture probes). A population of capture probes is also referred to as a “library” of capture probes. The capture probes generated using the methods described herein may be used for solution based capture to enrich for targets of interest.

FIG. 1C illustrates a representative capture probe 200 comprising a target-specific binding region 202 and a region 204 for binding to a capture reagent 300. In the embodiment shown in FIG. 1C, the capture reagent 300 is a universal adaptor oligonucleotide comprising a moiety 310 that binds to a capture reagent. The capture probes 200 generated using the methods of the present invention may be used to enrich a library for target nucleic acid regions of interest in a method referred to as solution based capture.

A representative method of solution based capture is illustrated in FIG. 2. The capture probes 200 (the capture probe 200 is representative of a population of capture probes) illustrated in FIG. 2 comprise a target-sequence specific binding region 202 and a capture reagent binding region 204 that hybridizes to a universal adaptor oligonucleotide 300 comprising a moiety 310 that binds to a capture reagent 400. As shown in FIG. 2 at step A, in one embodiment, solution based capture comprises contacting a library of DNA molecules 50 comprising a subpopulation of nucleic acid target insert sequences of interest 10 flanked by a first primer binding region 22 and a second primer binding region 32. The plurality of nucleic acid insert regions in the library include one or more target sequences 10, and can include enough different nucleic acid sequences to cover (i.e., represent) part or all of a source nucleic acid including, without limitation, the genome of an organism, a genomic locus, a cDNA library, a whole transcriptome of an organism, the exome of an organism and the like. As used herein the term “exome” refers to protein coding regions, promoters, known ncRNAs (non-coding RNAs) and UTRs, altogether comprising about 2% of the human genome.

As shown in FIG. 2 at step B, the target-specific binding region 202 of the target capture probe 200 binds to a substantially complementary target nucleic acid sequence 10, shown as an insert region 10 of a nucleic acid molecule 50 in a library of nucleic acid molecules. The universal adaptor oligonucleotide 300 is present at an equal concentration as the capture probes 200, and hybridizes to the capture reagent binding region 204. The moiety 310 (e.g., biotin) attached to the universal oligo adaptor 300 is then contacted with a capture reagent 400 (e.g., a magnetic bead) having a binding region 410 (e.g., streptavidin coating) and the complex is pulled out of solution with a sorting device 500 (e.g., a magnet) that binds to the capture reagent 400.

Any library of DNA molecules comprising a subpopulation of nucleic acid target insert sequences of interest may be enriched using the solution based capture methods described herein. In some embodiments, the library of DNA molecules comprises a plurality of distinct insert sequences flanked by a first primer binding region and a second primer binding region within a larger population of nucleic acid insert sequences flanked by the first primer binding region and the second primer binding region may be enriched for target sequences using the capture probes 200 generated using the methods disclosed herein. For example, a library of DNA molecules comprising a subpopulation of nucleic acid target insert sequences of interest flanked by a first primer binding region and a second primer binding region within a larger population of nucleic acid insert sequences flanked by the first primer binding region and the second primer binding region may be enriched using the capture probes generated using the methods of the invention. In some embodiments, the library of DNA molecules further comprises at least one anchor probe binding site, such as a flow cell binding site for binding to a flow cell sequencing platform, such as an Illumina Genome Analyzer for sequence analysis.

The use of solution-based capture to enrich a library allows for the efficient creation of resequencing samples (sequence-ready libraries) that are largely composed of target sequences, as demonstrated in Example 2.

The Design of the Target Capture Probe 200

The general design of the target capture probe 200 is described as follows. As shown in FIG. 1C, the target capture probe 200 comprises a target sequence-specific binding region 202 and a capture reagent region 204 for binding to a capture reagent 300.

The length of a target capture probe 200 is typically in the range of from 20 nucleotides to about 200 nucleotides, such as from about 20 nucleotides to about 150 nucleotides, such as from about 30 nucleotides to about 100 nucleotides, or such as from about 40 nucleotides to about 80 nucleotides.

The target-specific binding region 202 of the target capture probe 200 is typically from about 10 to about 150 nucleotides in length (e.g., 35 nucleotides, 50 nucleotides, 100 nucleotides) and is chosen to specifically hybridize to a target sequence of interest. In one embodiment, the target capture probe is about 60 to 80 nucleotides in length, comprising a target-specific binding region of about 20 to 40 nucleotides in length, such as about 35 nucleotides in length.

The target specific binding region 202 comprises a sequence that is substantially complementary (i.e., at least 95% identical, at least 96% identical, at least 97% identical, at least 98% identical, at least 99% identical, or 100% identical) to the target sequence of interest. Described in another way, for a target specific binding region 202 having a sequence with a length of from 10 to 100 nucleotides that is at least 95% complementary or at least 95% identical to a target sequence of interest, a region 202 that is from 20 nt to 35 nt in length may contain 1 mismatch, a region 202 that is from 40 nt to 50 nt in length may contain up to 2 mismatches, a region 202 that is from 60 nt to 70 nt may contain up to 3 mismatches, a region 202 that is from 80 nt to 90 nt may contain up to 4 mismatches, and a region 202 having a length of 100 nt may contain up to 5 mismatches with the target sequence.

In one embodiment, the method is used to generate a plurality of capture probes 200 each comprise a target-specific binding region 202 consisting of a sequence with a length of 35 nucleotides, that is at least 95% complementary, or at least 95% identical to a target sequence of interest (i.e., up to 1 mismatch with the target sequence).

The capture oligonucleotides may be designed to bind to a target region at selected positions spaced across the target region at various intervals. The capture oligo design and target selection process may also take into account genomic features of the target region such as genetic variation, G:C content, predicted oligo Tm, and the like. One of skill in the art can use art-recognized methods to determine the features of a target binding region that will hybridize to the target with minimal non-specific hybridization. For example, one of skill can determine experimentally the features such as length, base composition, and degree of complementarity that will enable a nucleic acid molecule (e.g., the target-specific binding region of a target capture probe) to specifically hybridize to another nucleic acid molecule (e.g., the nucleic acid target) under conditions of selected stringency, while minimizing non-specific hybridization to other substances or molecules. For example, for an exon target of interest, a target gene sequence is retrieved from a public database such as GenBank, and the sequence is searched for stretches of from 25 to 150 bp with a complementary sequence having a GC content in the range of 45% to 55%. The identified sequence may also be scanned to ensure the absence of potential secondary structure and may also be searched against a public database (e.g., a BLAST search) to ensure a lack of complementarity to other genes, as described in Example 3.

In some embodiments of the method, a set of capture probes (e.g., a library) is designed to specifically bind to target sequences across a genomic location, such as across a chromosomal region, and the capture probes are contacted with nucleic acid molecules from a total genomic library, or a whole-transcriptome library in order to analyze the whole transcriptome across the chosen genomic locus. In some embodiments of the method, a set of capture probes is designed to specifically bind to a plurality of target regions, such as the exons of a single gene, or multiple genes, such as at least 5 genes, at least 10 genes, at least 20 genes, at least 50 genes, at least 75 genes, at least 200 genes, at least 1000 genes, at least 10,000 genes, or more, as described in Examples 1-3. For example, as demonstrated in Example 3 herein, the methods according to this aspect of the invention were used to generate a set of capture probes comprising 1,148,286 distinct target-specific 35mer regions that were designed to capture all the exons from a total of 25,341 annotated genes from a sample containing nucleic acid sequences derived from a human.

In some embodiments of the method, a set of capture probes is designed to specifically bind to a genomic locus known to be associated with a clinical outcome or disease, or disease risk.

In some embodiments, the methods of the invention are used to capture and sequence a modified or mutated target, such as to determine the presence of a particular single nucleotide polymorphism (SNP), or deletion, addition, or other modification. In accordance with such embodiments, the set of target capture probes are typically designed such that there is a very dense array of capture probes that are closely spaced together such that a single target sequence, which may contain a mutation, will be bound by multiple capture probes that overlap the target sequence. For example, capture probes may be designed that cover every base of a target region, on one or both strands, (e.g., head to tail), or that are spaced at intervals of every 2, 3, 4, 5, 10, 15, 20, 40, 50, 90, 100, or more bases across a sequence region.

As another example, the selection of the target capture probes over a target region of interest is based on the size of the target region. For example, for a target region of less than 100 nucleotides in length, capture probes (either sense, antisense, or both) are typically designed to hybridize to target sequences spaced apart by from 0 to 100 nucleotides, such as every 45 nucleotides, or every 35 nucleotides. As another example, for a target region greater than 200 nucleotides, capture probes (either sense, antisense, or both) are typically designed to hybridize to target sequences spaced apart by from 0 to 200 nucleotides, such as at 45 to 65 nucleotide intervals, or at higher density coverage such as every 35 nucleotide intervals. In one embodiment, for a target region greater than 200 nucleotides (e.g., a 200,000-nucleotide target region), a set of sense and antisense capture probes are designed that are each about 35 nucleotides in length and are spaced about 45 nucleotides apart across the target region (alternating sense/antisense) in order to saturate the region (e.g., “tile” across the region of interest).

In one embodiment, a library of target specific probes are designed to bind to a desired target with high density coverage, such that at least one capture probe binds to at least every 35 nucleotide region of the target sequence. In one embodiment, a library of target specific probes are designed to bind to every nucleotide of the target, with alternating binding regions on the sense and antisense strands of the target sequence.

Referring now to FIG. 1C, in one embodiment, the target capture probes 200 each comprise a capture reagent binding region 204 that hybridizes to a universal adaptor oligonucleotide 300 comprising a moiety 310 that binds to a capture reagent 400. The capture reagent binding region 204 of the target capture probe 200 is typically from about 10 to about 50 nucleotides in length (e.g., 10 nucleotides, 15 nucleotides, 20 nucleotides, 35 nucleotides) and is chosen to specifically hybridize to a universal adaptor oligonucleotide comprising a moiety 310 that binds to a capture reagent. In one embodiment, the target capture probe is about 60 to 80 nucleotides in length, comprising a capture reagent binding region of about 10 to 40 nucleotides in length, such as about 35 nucleotides in length.

In operation, as shown in FIG. 2, the target-specific binding region 202 of the target capture probes bind to a complementary or substantially complementary nucleic acid sequence contained in a nucleic acid target 10 (i.e., an insert of a nucleic acid molecule in a library, or a genomic region of nucleic acids isolated from a sample). A universal adaptor oligonucleotide 300 is present at an equal concentration as the capture probes 200, and hybridizes to the capture reagent binding region 204. The moiety 310 (e.g., biotin) attached to the universal adaptor oligonucleotide 300 is then contacted with a capture reagent 400 (e.g., a magnetic bead) having a binding region 410 (e.g., streptavidin coating) and the complex is pulled out of solution with a sorting device 500 (e.g., a magnet) that binds to the capture reagent 400.

As shown in FIG. 3, the method 600 of generating a population of target-specific capture probes in accordance with one embodiment of the invention, includes the step 610 of providing a starting population of double-stranded capture probe precursors, each precursor comprising a capture probe region 200 (comprising a target-sequence specific binding region 202 and a capture reagent binding region 204), flanked by a 5′ flanking region 210 and a 3′ flanking region 220. The 5′ flanking region 210 comprises a first processing site and the 3′ flanking region 220 comprises a second processing site, wherein the first and second processing sites are selected to generate ligation substrates capable of ligation into head-to-tail concatemers. An exemplary double-stranded capture probe precursor 230 is illustrated in FIG. 4 at step A. As shown in FIG. 4 at step A, the exemplary double-stranded capture probe precursor 230 comprises a capture probe region 200 flanked on the 5′ end by flanking region 210 comprising a first processing site (e.g., a first Bsm1 site) and is flanked on the 3′ end by flanking region 220 comprising a second processing site (e.g., a second Bsm1 site). In some embodiments, the 3′ flanking region 220 further comprises a third processing site (e.g., a Psi1 site or a HindIII site) selected to precisely cleave off the 3′ flanking region.

The 5′ flanking region 210 is typically from about 4 to about 30 nucleotides in length, such as from about 5 to about 15 nucleotides in length, or from about 5 to 10 nucleotides in length. The nucleotide sequence of the 5′ flanking region 210 is chosen to provide a first processing site, such as a first restriction enzyme recognition site, such as a type II restriction endonuclease (e.g., Bsm1), to generate the desired nucleotide overhang at the first end of the double-stranded precursor molecule for ligation in a head to tail configuration, and for precise cleavage of the 5′ flanking region 210 from the capture probe 200 region.

The 3′ flanking region 220 is typically from about 4 to about 30 nucleotides in length, such as from about 5 to about 25 nucleotides in length, or from about 10 to 20 nucleotides in length. The nucleotide sequence of the 3′ flanking region 220 is chosen to provide a second processing site, such as a second restriction enzyme recognition site (e.g., Bsm1), to generate the desired nucleotide overhang at the second end of the double-stranded molecule for ligation. In some embodiments, the 3′ flanking region 220 further comprises an additional processing site (e.g., a third restriction enzyme recognition site, such as Psi1 or HindIII) for precise cleavage of the 3′ flanking region 220 from the capture probe 200 region.

As illustrated in FIG. 4, step B, the population of double-stranded capture probe precursors 230 are processed into ligation substrates that form head-to-tail concatemers upon ligation with one another. Accordingly, the first processing site in the 5′ flanking region and the second processing site in the 3′ region are chosen such that upon digestion of the first and second processing sites in the double-stranded precursor 230, nucleotide overhangs are left on each end of the double-stranded precursor that can only ligate with one another in a head to tail configuration (e.g., a “CC” overhang on the first end of the molecule and a “GG” overhang on the second end of the molecule, as illustrated in FIG. 4, step C).

It will be understood by one of skill in the art that Bsm1 is a non-limiting example of a type II restriction endonuclease that recognizes an asymmetric sequence and cleaves outside of that asymmetric sequence to yield the desired nucleotide overhangs at the first end and the second end of the double-stranded precursor molecule for ligation in a head to tail configuration. For example, dozens of type II restriction endonuclease enzymes are commercially available and known to those of skill in the art (see, e.g., New England Biolabs catalogue and REBASE web site) from which one of skill in the art could design a first processing site in the 5′ flanking region and a second processing site in the 3′ flanking region to provide ligation substrates that could only ligate in a head to tail configuration.

The double-stranded capture probe precursor 230 for use in various embodiments of the methods of the invention may be generated using a variety of methods. In one embodiment, the double-stranded capture probe precursor 230 is generated by synthesizing pairs of single-stranded complementary sense and antisense oligonucleotides comprising the full length sequence of the capture probe precursor 230 and annealing the strands together to form the double-stranded capture probe precursor. In another embodiment, a ligation ready, double-stranded capture probe precursor 230 having from one to four nucleotide overhangs on each end, is generated by annealing a pair of synthesized complementary strands together to produce the desired nucleotide overhang that will result in head-to-tail concatemers upon ligation.

In another embodiment, as shown in FIG. 4, step A, and demonstrated in Example 1, a population of double-stranded precursor molecules 230 is generated by first synthesizing a population of single-stranded oligonucleotide precursors 100 comprising a 5′ flanking region 210, a target-specific binding region 202, and a portion of the capture reagent binding region 204 (e.g., at least 10 to 20 nucleotides) and annealing the synthesized population of oligos 100 with a common reverse primer oligonucleotide 150 to form the double-stranded capture probe precursor molecules 230. As illustrated in FIG. 4, the common reverse primer oligo 150 is designed to hybridize to the region 204 for binding to a capture reagent on the synthesized oligos 100, and includes the complement of the remaining portion of the region 204, as well as the complement of the 3′ flanking region 220. An advantage to the second strand fill in reaction with the reverse primer oligo 150 is the ability to change the 3′ flanking region through the use of a reverse primer oligo 150 with different sequences in order to incorporate different restriction enzyme sites or other desired sequences in the 3′ flanking region 220 of the double-stranded capture probe precursor molecule 230. For example, other sequences that could be included in the 3′ flanking region 220 include primer binding sites, protein binding sites (e.g., for binding a prokaryotic polymerase such as T7 RNA polymerase), modified nucleotides for purification (e.g., biotinylated residues), or methylated 5-methylated 5-methyl-cytosine residues for resistance to bisulphate conversion.

Referring again to FIG. 3, at step 620, the population of double-stranded capture probe precursors 230 are processed into ligation substrates that form head-to-tail concatemers upon ligation with one another, as illustrated in FIG. 4, step B. One of skill in the art can use art-recognized methods to determine the sequence of a 5′ flanking region 210 and a 3′ flanking region 220 that will provide suitable processing sites, such as restriction endonuclease recognition sites, for generating a first overhang of from 1 to 12 nucleotides on the first end and a second overhang of from 1 to 12 nucleotides on the second end of the double-stranded capture probe precursor 230. In a non-limiting example, as described in Example 1, the double-stranded capture probe precursors 230 are designed to include a 5′ flanking region 210 comprising a first restriction enzyme site for Bsm1, in order to create a two nucleotide “GG” overhang at the first end of the precursor 230 molecule, and a 3′ flanking region 220 comprising a second restriction enzyme site for Bsm1, in order to create a two nucleotide “CC” overhang at the second end of the precursor 230 after digestion with Bsm1 to facilitate ligation into head-to-tail concatemers, as illustrated in FIG. 4, step B.

At step 630, the processed capture probe precursors are ligated to generate head-to-tail concatemers 240, as illustrated in FIG. 4, step C. As illustrated in FIG. 4, step C, it is noted that the ligation reaction naturally drives towards the formation of circularized templates due to the fact that in very dilute solutions of free ends, the probability of self-ligation (circularization) becomes higher than the probability of finding a separate free end.

At step 640, the head-to-tail concatemers 240 are amplified using any suitable amplification method, such as PCR amplification, in vitro transcription, Klenow, or isothermal amplification.

In one embodiment, the amplification of the head-to-tail concatemers 240 is carried out using isothermal amplification with either Bacillus subtilis phage phi29 polymerase (hereafter referred to as “phi29” polymerase) or Bacillus stearothermophilus (Bst) DNA polymerase large fragment, 5′→3′ exo⁻ (hereafter referred to as “Bst DNA polymerase”). Isothermal amplification is based on random priming of denatured DNA, followed by strand-displacement synthesis at constant temperature, wherein multiple primers are extended over tens of kilobases, as described in Lage et al., Genome Res 13:294-307 (2003), incorporated herein by reference. The single-stranded DNA generated by strand displacement is targeted by new random priming events, and these new strands are elongated in the opposite direction, resulting in a hyperbranched network of amplified head-to-tail concatemers, as shown in FIG. 4, step D.

In one embodiment, as illustrated in FIG. 4, step D, a random primer 250 is used for amplification using the strand displacement polymerase technology found in the TEMPLIPHI isothermal amplification kit (phi29) (GE Life Sciences, Piscataway N.J.). In one embodiment, the random primer 250 comprises a random 7-mer amplification primer with an additional two nitroindole residues at the 5′ end and a phosphorothioate linkage at the 3′ end: 5′[nitroindole]2-[N]6-(phosphothioate)-N (SEQ ID NO:77), as described by Lage et al., Genome Res 13:294-307 (2003).

At step 650, the amplified head-to-tail concatemers are processed to release monomer double-stranded capture probe precursors. In one embodiment, as shown in FIG. 4, step E, the concatemers are processed by restriction enzyme cleavage using a type II restriction enzyme that recognizes a site present in the 5′ and 3′ flanking regions, such as Bsm1.

At step 660, the amplified monomer double-stranded precursor molecules are processed to remove the 5′ and/or 3′ flanking regions. In one embodiment, as shown in FIG. 4, step E and step F, the 5′ flanking region is precisely removed by digestion with Bsm1 and the 3′ flanking region is precisely removed by digestion with either Psi1 or HindIII.

At step 670, the monomer double-stranded precursor molecules are further processed to selectively remove the complementary strand of the capture probe to produce a population of single-stranded target specific capture probes 200.

In one embodiment, as shown in FIG. 4, at step E, the Bsm1 digested monomers are treated with alkaline phosphatase to remove the 5′ terminal phosphates. At step F, the precise 3′ ends of capture probes are then liberated by digestion with either PsiI or HindIII, which each leaves 5′ terminal phosphates on the complementary strands. At step G, single-strand capture probe 200 libraries containing only the target specific region 202 and the capture reagent binding region 204 are generated by digesting the double-stranded monomer capture probe precursors with an enzyme, such as Lambda exonuclease, that specifically degrades dsDNA by attacking at 5′ phosphate sites and selectively digests away the non-capture complementary strand, thereby converting the dsDNA into ssDNA suitable for use as capture probes.

Alternatively, the non-capture complementary strand may be removed by first adding exonuclease resistant adaptors to the capture probe strand and degrading away the non-capture complementary strand with any suitable double-strand DNA specific exonuclease, such as Exonuclease III. In another example, the capture strand may be selectively amplified by adding an amplification primer binding site in the 5′ flanking region 210 of the capture probe precursor 230, and selectively amplifying the plurality of amplified capture probe monomers 270 with an amplification primer that binds to the amplification primer binding site.

FIG. 5 illustrates further details of the method illustrated in FIG. 4, with regard to a library of single-stranded nucleic acid probes having distinct predetermined sequences A, B, C, and D, such as target-specific capture probes. It will be understood that the method of generating the library comprising probes A, B, C and D is representative for generating a library comprising at least 1,000, such as at least 5,000, such as at least 10,000, such as at least 50,000, such as at least 100,000, such as at least 1,000,000 or more single-stranded nucleic acid probes having predetermined nucleic acid sequences, such as target-specific capture probes. As shown in FIG. 5, step A, a library of single-stranded precursor molecules 100 is synthesized on a substrate 102, the synthesized population comprising a 5′ flanking region 210, a target specific binding region 202 and a portion of the capture reagent binding region 204, wherein each of the target specific binding regions comprises a nucleic acid sequence selected to bind to target sequence A, B, C, or D.

The synthesized library of single-stranded precursor molecules 100 is then cleaved off the substrate 102, annealed to a common reverse primer 150 and extended with Klenow, to generate a library of double-stranded capture probe precursor molecules 230 (best illustrated in FIG. 4). Accordingly, the library of double-stranded nucleic acid precursor molecules 230 comprises a 5′ flanking region 210 that is essentially identical to every other 5′ flanking region in the library, a 3′ flanking region 220 that is essentially identical to every other 3′ flanking region in the library, and a probe region 200 comprising a nucleic acid sequence 202 that is different from a least a portion of the nucleic acid sequence 202 present in every other probe region 200.

Referring again to FIG. 5, at step C, the 5′ and 3′ regions of the double-stranded capture probe precursor molecules 230 are then processed to generate ligation substrates and ligated to form head-to-tail concatemers 240 as described with reference to FIG. 4. As illustrated in FIG. 5, step C, the head-to-tail concatemers 240 each comprise a plurality of ligated monomer capture probe regions (A, B, C, D) that are randomly ligated together. At step D, the circularized concatemers 240 are amplified via isothermal amplification with random primers 250, resulting in a plurality of amplified concatemers 260 (best shown in FIG. 4), which are then processed (e.g., with Bsm1) at Step E to generate a library of amplified capture probe monomers 270, which are further processed to remove the non-capture complementary strands, as described with reference to FIG. 4, into a library of single-stranded capture probes 200 comprising a plurality of different target specific binding regions 202 (e.g., that specifically bind to targets A, B, C, and D).

In some embodiments of the methods described herein, a library of capture probe precursors in the form of head-to-tail concatemers, with reference to FIG. 4, step C (e.g., the ligation mixture), or amplified head-to-tail concatemers, with reference to FIG. 4, step D (e.g., the amplified reaction) may be stored at −20° C. for a period of time from several hours up to 6 months or longer, for use as a template in a subsequent amplification reaction, in order to generate additional yields of the capture probe library 200, thus avoiding the need for repeated oligonucleotide synthesis of a particular library of capture probes.

The population of single-stranded capture probes 200 can be used in solution based capture methods as described herein. As demonstrated in Example 2, and shown in FIG. 6, it has been determined that the single-stranded capture probes generated from amplified head-to-tail concatemers, in accordance with the methods of the invention, work at least as well for solution based capture methods as a population of corresponding capture probes that were generated by direct oligonucleotide synthesis. Therefore, it is demonstrated that the methods are useful for generating a library of uniformly amplified single-stranded capture probes from a starting population of double-stranded capture probe precursor molecules.

The level of representation of expected nucleic acid sequences in a library generated according to the methods of the invention typically has a variation of less than about 30% (such as a variation of less than about 20%). The level of representation of expected nucleic acid sequence in the final single stranded capture probe library 200 may be assessed using various methods. For example, as described in Example 2, a capture probe library 200 may be used for solution-based capture of a set of targets (e.g., the 13 exons of the AKT gene), and the standard deviation of the exon to exon capture efficiency, expressed as a percentage of the fold-enrichment can be determined, as shown in FIG. 6, thereby providing an indirect measure of the representation of the expected nucleic acid sequences in the capture probe library 200. As another example, quantitative PCR assays may be carried out for representative target sequences at an early step in the method shown in FIG. 4, such as after initial synthesis of the single-stranded precursor nucleic acid molecules 100, or after formation of the double-stranded precursor molecules 230, and compared to quantitative PCR results obtained from the final population of single-stranded capture probes 200 to verify uniform representation of the target sequence. As another example, a test sample that is representative of the final population of single-stranded capture probes 200 can be labeled and hybridized to a substrate comprising a population of nucleic acid molecules comprising the set of predetermined sequences expected to be present in the final population of single-stranded capture probes 200, and the representation of the expected nucleic acids in the test sample is evaluated by analyzing the resulting hybridization pattern.

Oligonucleotide Synthesis

DNA synthesis of the various oligonucleotides of the invention (e.g., single-stranded nucleic acid molecules having predetermined sequences, capture probe precursors and universal adaptor oligonucleotides) can be carried out by any art-recognized chemistry, including phosphodiester, phosphotriester, phosphate triester, or N-phosphonate and phosphoramidite chemistries (see, e.g., Froehler et al., Nucleic Acid Res. 14:5399-5407, 1986; McBride et al., Tetrahedron Lett. 24:246-248, 1983). Methods of oligonucleotide synthesis are well known in the art and generally involve coupling an activated phosphorous derivative on the 3′ hydroxyl group of a nucleotide with the 5′ hydroxyl group of the nucleic acid molecule (see, e.g., Gait, Oligonucleotide Synthesis: A Practical Approach, IRL Press, 1984).

A population of nucleic acid molecules can be synthesized on a substrate by any art-recognized means including, for example, photolithography (see, Lipshutz et al., Nat. Genet. 21(1 Suppl):20-24, 1999) and piezoelectric printing (see, Blanchard et al., Biosensors and Bioelectronics 11:687-690, 1996). In some embodiments, nucleic acid molecules are synthesized in a defined pattern on a solid substrate to form a high-density microarray. Techniques are known for producing arrays containing thousands of oligonucleotides comprising defined sequences at defined locations on a substrate (see, e.g., Pease et al., Proc. Nat'l. Acad. Sci. 91:5022-5026, 1994; Lockhart et al., Nature Biotechnol. 14:1675-80, 1996; and Lipshutz et al., Nat. Genet. 21 (1 Suppl):20-4, 1999).

In some embodiments, populations of nucleic acid molecules are synthesized on a substrate, to form a high density microarray, by means of an ink jet printing device for oligonucleotide synthesis, such as described by Blanchard in U.S. Pat. No. 6,028,189; Blanchard et al., Biosensors and Bioelectrics 11:687-690 (1996); Blanchard, Synthetic DNA Arrays in Genetic Engineering, Vol. 20, J. K. Setlow, Ed. Plenum Press, New York at pages 111-123; and U.S. Pat. No. 6,028,189 issued to Blanchard. The nucleic acid sequences in such microarrays are typically synthesized in arrays, for example, on a glass slide, by serially depositing individual nucleotide bases in “microdroplets” of a high surface tension solvent such as propylene carbonate. The microdroplets have small volumes (e.g., 100 picoliters (pL) or less, or 50 pL or less) and are separated from each other on the microarray (e.g., by hydrophobic domains) to form surface tension wells which define the areas containing the array elements (i.e., the different populations of nucleic acid molecules). Microarrays manufactured by this ink-jet method are typically of high density, typically having a density of at least about 2,000 different nucleic acid molecules per 1 cm². The nucleic acid molecules may be covalently attached directly to the substrate, or to a linker attached to the substrate at either the 3′ or 5′ end of the polynucleotide. Exemplary chain lengths of the synthesized nucleic acid molecules suitable for use in the present methods are in the range of about 20 to about 100 nucleotides in length, such as 50 to 100, 60 to 100, 70 to 100, 80 to 100, or 90 to 100 nucleotides in length. In some embodiments, the nucleic acid molecules are in the range of 80 to 100 nucleotides in length.

Exemplary ink jet printing devices suitable for oligonucleotide synthesis in the practice of the present invention contain microfabricated ink-jet pumps, or nozzles, which are used to deliver specified volumes of synthesis reagents to an array of surface tension wells (see, Kyser et al., J. Appl. Photographic Eng. 7:73-79, 1981).

In some embodiments, a population of nucleic acid molecules is synthesized to form a high-density microarray. A DNA microarray, or chip, is an array of nucleic acid molecules, such as synthetic oligonucleotides, disposed in a defined pattern onto defined areas of a solid support (see, Schena, BioEssays 18:427, 1996). The arrays are preferably reproducible, allowing multiple copies of a given array to be produced and easily compared with each other. Microarrays are typically made from materials that are stable under nucleic acid molecule hybridization conditions. In some embodiments, the nucleic acid molecules on the array are single-stranded DNA sequences. Exemplary microarrays and methods for their manufacture and use are set forth in T. R. Hughes et al., Nature Biotechnology 19:342-347, April 2001, which publication is incorporated herein by reference.

In some embodiments, the methods of the invention utilizes oligonucleotides that are synthesized on a multiplex parallel DNA synthesis system based on an integrated microfluidic microarray platform for parallel production of oligonucleotides, wherein the DNA synthesis system utilizes photogenerated acid chemistry, parallel microfluidics and a programmable digital light controlled synthesizer, as described in U.S. Patent Pub. No. 2007/0059692, Gao et al., Biopolymers 73:579-596 (2004), and Zhou et al., Nucleic Acids Research 32(18):5409-5417 (2004), each of which is incorporated herein by reference.

In some embodiments, the methods of the invention utilize synthesized oligonucleotides that are cleaved off a substrate, such as a microarray. The synthesized nucleic acid molecules can be harvested from the substrate by any useful means. In some embodiments, the portion of the nucleic acid molecule that is directly attached to the substrate, or attached to a linker that is attached to the substrate, is attached to the substrate or linker by an ester bond which is susceptible to hydrolysis by exposure to a hydrolyzing agent, such as hydroxide ions, for example, an aqueous solution of sodium hydroxide or ammonium hydroxide. The entire substrate can be treated with a hydrolyzing agent, or alternatively, a hydrolyzing agent can be applied to a portion of the substrate. For example, a silane linker can be cleaved by exposure of the silica surface to ammonium hydroxide, yielding various silicate salts and releasing the nucleic acid molecules with the silane linker into solution. In some embodiments, ammonium hydroxide can be applied to the portion of a substrate that is covalently attached to the nucleic acid molecules, thereby releasing the nucleic acid molecules into the solution (see, Scott and McLean, Innovations and Perspectives in Solid Phase Synthesis, 3^(rd) International Symposium, 1994, Mayflower Worldwide, pp. 115-124).

In another aspect, the present invention provides a method for enriching a library for target nucleic acid regions of interest. The methods according to this aspect of the invention comprise: (a) amplifying a plurality of head-to-tail concatemers formed from ligating a population of double-stranded nucleic acid precursor molecules, wherein each double-stranded precursor molecule in the starting population comprises a target capture probe region comprising (i) a target-specific binding region comprising a nucleic acid sequence that is at least 95% identical to at least a portion of the sense or antisense strand of a target nucleic acid sequence of interest and (ii) a region for binding to a capture reagent; wherein the target capture region is flanked on the 5′ end by a 5′ flanking region comprising a first processing site and is flanked on the 3′ end by a 3′ flanking region comprising a second processing site; (b) processing the amplified head-to-tail concatemers to release double-stranded monomer precursor molecules; (c) selectively removing the complement strand of the double-stranded monomer precursor molecules to generate a population of single-stranded capture probes, each capture probe comprising (i) a target-specific binding region comprising a nucleic acid sequence that is at least 95% identical to at least a portion of the sense or antisense strand of a target nucleic acid sequence of interest and (ii) a region for binding to a capture reagent; (d) contacting the population of single-stranded capture probes with a library comprising at least one target nucleic acid sequence of interest under conditions that allow binding between the capture probes and the at least one nucleic acid target region of interest, to form a mixture comprising a plurality of complexes between target regions of interest and capture probes; (e) contacting the mixture of step (d) with a capture reagent and separating the capture reagent bound complex from the mixture; and (f) eluting the target regions of interest from the capture reagent bound complex.

The steps (a) to (c) may be carried out as previously herein described. The steps (d) to (f) of enriching a library for target sequences with the population of single-stranded capture probes may be carried out as illustrated in FIG. 2. As shown in FIG. 2A, solution-based capture is carried out by first annealing the library of single-stranded capture probes 200, each capture probe comprising a target specific region 202 that hybridizes to a target sequence contained in a library insert, with a library of nucleic acid molecules 50 comprising nucleic acid target insert sequences of interest 10 flanked by a first primer binding region 22 on one end and a second primer binding region 32 on the other end. As further shown in FIG. 2, step A, in one embodiment, the library of nucleic acid molecules 50 is annealed with a combination of a library of single-stranded capture probes 200 each comprising a region 204 that hybridizes to a universal adaptor oligo 300 and an equimolar amount of universal adaptor oligos 300 comprising a moiety 310 for binding to a capture reagent 400.

The annealing step is typically carried out by mixing a molar excess of capture probes (or capture probes plus universal adaptor oligos) with the library in a high salt solution comprising from 100 mM to 2 M NaCl (osmolarity=200 to 4000 molar). An exemplary high salt solution for annealing is 10 mM Tris pH 7.6, 0.1 mM EDTA, 1 M NaCl (osmolarity=2000 molar). The nucleic acid molecules in the mixture are then denatured (i.e., by heating to 94 degrees) and allowed to cool to room temperature. In one embodiment, the annealing step is carried out in a high salt solution comprising from 100 mM to 2 M NaCl with the addition of 0.1% triton X100 (or Tween or NP40) nonionic detergent.

An amount of capture reagent 400 is added to the annealed mixture sufficient to generate a plurality of complexes each containing a nucleic acid molecule, a capture probe (or a capture probe and a universal adaptor oligo), and a capture reagent. This step is carried out in a high salt solution comprising from 100 mM to 2 M NaCl (osmolarity=200 to 4000 molar). An exemplary high salt solution for anneal is 10 mM Tris pH 7.6, 0.1 mM EDTA, 1 M NaCl (osmolarity=2000 molar). The mixture is incubated at room temperature with mixing for about 15 minutes.

The complexes formed are then isolated or separated from solution with a sorting device 500 (e.g., a magnet) that pulls or sorts the capture reagent 400 out of solution.

The sorted complexes bound to the capture reagent 400 are washed with a low salt wash buffer (less than 10 mM NaCl, and more preferably no NaCl) to remove non-target nucleic acids. An exemplary low salt wash buffer is 10 mM Tris pH 7.6, 0.1 mM EDTA (osmolarity=10 millimolar). In some embodiments, the low salt wash optionally contains from 15% to 30% formamide, such as 25% formamide (osmolarity=6.3 molar). For each wash step, the capture reagent 400 bound to the complexes (e.g., magnetic beads) are resuspended in the low salt wash buffer and rocked for 5 minutes, then sorted again with the sorting device (magnet). The wash step may be repeated 2 to 4 times.

The nucleic acid molecules containing the target sequences are then eluted from the complexes bound to the capture reagent as follows. The washed complexes bound to the capture reagent 400 are resuspended in water, or in a low salt buffer (i.e., osmolarity less than 100 millimolar), heated to 94° C. for 30 seconds, the capture reagent (e.g., magnetic beads) is pulled out using a sorting device (e.g., magnet), and the supernatant (eluate) containing the target nucleic acid molecules is collected.

The eluate may optionally be amplified in a PCR reaction with a first PCR primer that binds to the first primer binding site 22 in the first linker and a second PCR primer that binds to the second primer binding site 32 in the second linker, producing an enriched library which can be optionally sequenced.

In another aspect, the present invention provides kits for generating a population of single-stranded nucleic acid molecules from a population of precursor double-stranded molecules. The kits according to this aspect of the invention are useful for carrying out various embodiments of the methods of the invention described herein. The kits in accordance with this aspect of the invention comprise (a) a plurality of random 7-mer oligonucleotide primers, (b) at least one of phi29 polymerase or Bst DNA polymerase large fragment 5′-3′ exo-; and (c) a lambda exonuclease enzyme. In some embodiments of the kit, the plurality of random 7-mer oligonucleotide primers each comprise an additional two nitroindole residues at the 5′ end and a phosphorothioate linkage at the 3′ end (SEQ ID NO:77). In some embodiments, the kit may further comprise at least one of the following: an alkaline phosphatase enzyme, at least one type II restriction enzyme, a DNA ligase, and a DNA polymerase enzyme (Klenow).

In an embodiment of the kit comprising phi29 polymerase, the kit may optionally further comprise one or more of the following reagents: (i) a phi29 concentrated stock reaction buffer comprising at least one of the following: Tris-HCL (e.g., at 50 mM for 10× buffer), (NH₄)₂SO₄ (e.g., at 10 mM for 10× buffer), MgCl₂ (e.g., 10 mM for 10× buffer) and dithiothreitol (e.g., 4 mM for 10× buffer); (ii) a concentrated stock of dNTPs (e.g. from 100 μM to 10 mM dNTPs); and (iii) a reducing agent, such as dithiothreitol. In further embodiments, the kit may optionally comprise at least one or more of the following; a common reverse primer oligo 150 designed to hybridize to the region 204 for binding to the synthesized oligos 100, Klenow enzyme, at least one Type II Restriction Enzyme, ligase, and alkaline phosphatase.

The following examples merely illustrate the best mode now contemplated for practicing the invention, but should not be construed to limit the invention.

EXAMPLES

All of the compositions and methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. In some cases, the compositions and methods of this invention have been described in terms of embodiments, however these embodiments are in no way intended to limit the scope of the claims, and it will be apparent to those of skill in the art that variations may be applied to the compositions and/or methods and in the steps or in the sequence of steps of the methods described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be apparent that certain components which are both chemically and physiologically related may be substituted for the components described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.

Example 1

This Example demonstrates a method for uniformly amplifying a library of target capture probes specific for the AKT gene designed for solution based capture of the 13 exons of the AKT gene.

Rationale:

Several recent studies describe the complete resequencing of human genomes (Wang et al., Nature 456:60 (2008); Bentley et al., Nature 456:53 (2008); Ley et al., Nature 456:66 (2008)). One in particular describes the full genome sequence of a tumor and normal adjacent tissue (Ley et al. (2008)). Remarkably, the authors distill their analysis of the complete human genome to ten mutated genes, highlighting the point that a small percentage of human genome sequence variations, including the protein coding regions and some surrounding flanking sequences, is interpretable. At the opposite end of the spectrum, candidate gene resequencing strives to test phenotype to genotype hypotheses by seeking sequence variation in specific genes that are thought to influence traits. The caveat is that the selection of candidate genes can be somewhat arbitrary. The ideal solution is one in which the interpretable regions, referred to as the “exome” of the genome, can be selectively resequenced. As used herein, the term “exome” refers to the collection of genomic segments that include protein coding regions, exons, promoters, known ncRNAs (non-coding RNAs) and UTRs, altogether comprising about 2% of the human genome. Interestingly, if the exome could be captured from a sample, then the sequencing data from a single flow cell from a highly parallel sequencing technology (e.g., a single Illumina GAII flow cell) would be adequate to address all of the diploid variation present in the exomic fraction of that sample.

Solution-based capture of the human exome would require ˜2 million oligonucleotides. Even with state-of-the-art, high-throughput oligo synthesis stations, the cost of each capture oligo is $7 and therefore the cost of synthesizing an exome solution based capture library would be approximately $14 million. An alternative—synthesized oligonucleotide libraries cleaved from microarrays, also initially appears to be an untenable solution due to the high cost involved to obtain sufficient yields of material. Each microarray typically produces about 50,000 individual oligonucleotide sequences that are high-quality, cleavable oligos in picomole quantities (e.g., commercially available from Agilent, Santa Clara, Calif.). Thus, the cost of synthesizing a library of exome capture oligonucleotide probes from microarrays (requiring ˜40 arrays at an estimated cost of $400,000) is substantially reduced relative to conventional oligonucleotide synthesis, however, the yield of material produced by microarray synthesis is sufficient for only four solution based capture experiments. Moreover, the use of high density probe coverage (i.e., more than 4 probes per exon, or at least one capture probe per 35 nt), as shown in FIG. 1B, is preferable for solution based capture in comparison to low density probe coverage (i.e., less than 3 probes per exon, or less than one capture probe per 35 nt), as shown in FIG. 1A. The use of high density probe coverage is preferred in order to ensure that desired targets are enriched, thereby reducing the depth of re-sequencing required of the enriched material. However, such high density probe coverage calls for a greater number of capture oligonucleotides, which significantly inflates the overall expense of technologies that utilize solution based capture methods. Therefore, a need exists to provide a cost-effective method for generating high quality custom oligonucleotide libraries with sufficient yield for applications such as solution based capture.

This Example demonstrates a method for uniformly amplifying a library of synthesized capture oligonucleotides for use in solution based capture methods.

Design of Capture Probe Oligonucleotide Precursors for Amplification and Processing into a Library of Capture Probes:

As shown in FIG. 1C, capture probes 200 may be used to capture nucleic acid molecules comprising target sequences 10 from a mixture of target and non-target nucleic acid molecules. As shown in FIG. 1C, capture probes 200 comprise a target-specific binding region 202 and a region 204 for binding to a capture reagent 300. In the embodiment shown in FIG. 1C, the capture reagent 300 is a universal adaptor oligonucleotide comprising a moiety 310 that binds to a capture reagent, thereby resulting in a tri-molecular solution based capture complex.

Design of Capture Probe Precursor Oligonucleotides:

FIG. 4 at Step A illustrates the general structure of a double-stranded capture probe precursor 230 prior to processing into a single-stranded capture probe 200. The double-stranded capture probe precursor was designed to ensure that the ligation of a plurality of double-stranded capture oligonucleotide probe precursors 230 would result in head-to-tail concatemers 240, as illustrated at Step C, which were then used as templates for amplification, as illustrated at Step D, followed by cleavage into monomers and alkaline phosphatase, as illustrated at Step E, followed by selective degradation of the complementary strand of the capture probe precursor, as shown at Step G, thereby generating a single-stranded capture probe 200 comprising a target-specific binding region 202 and a region 204 for binding to a capture reagent 300, without the 5′ flanking region 210 and the 3′ flanking region 220.

As shown in FIG. 4, Step A, the general structure of the double-stranded capture probe precursor 230 includes a 5′ flanking region 210 comprising a first restriction enzyme site for creating a first nucleotide overhang for ligation (e.g., Bsm1); a capture probe region 200 comprising a target specific hybridizing region 202 and a universal capture oligo hybridizing region 204, and a 3′ flanking region 220 comprising a second restriction enzyme site (e.g., Bsm1) for creating a second nucleotide overhang for ligation, and further comprising a third restriction enzyme site (e.g., Psi or HindIII), for precisely cleaving off the 3′ flanking region.

Design of the Target-Specific Region 202 of the Capture Probe 200:

In this Example, a 64 oligonucleotide library was synthesized for high density solution based capture of the 13 exon AKT1 gene (NM_(—)005163). As illustrated in FIG. 1B, a library of oligonucleotide capture probes were designed such that each target AKT1 exon had at least 4 or more probes (high density), and the capture probes alternated in strand orientation and were perfectly head to tail with no spaces in between, each having the sequence identical to the corresponding exon sequences 1-13 from the AKT1 gene. The target specific binding regions 202 of the capture probes in the AKT1 capture probe library had a length of 35 nt, as shown below in TABLE 1.

Design of the Region 204 for Binding to a Capture Reagent 300:

The AKT1 capture probes 200 in the library were designed such that each final processed single-stranded capture probe had a 34 nucleotide common region 204 for binding to the universal oligo adaptor capture reagent 300.

As shown in FIG. 4, Step A, in order to further reduce costs of oligonucleotide synthesis, in this Example, the initial population of synthesized oligos 100 included the 5′ flanking region 210, the target specific binding regions 202, and only a portion (15 nt of the 34 nt region) of the common region 204 for binding to a capture reagent 300. As shown in FIG. 4, Step A, the 15 nt of the region 204 (5′ ACGCGTGGCGGATGT 3′ (SEQ ID NO:1)) binds to a region of the reverse primer 150 that was used to anneal and fill-in the library synthesized oligos 100, thereby resulting in the double-stranded capture probe precursors 230.

Design of the 5′ Flanking Region 210 of the Capture Probe Precursor 230:

The double-stranded AKT1 capture probe precursors 230 were designed to include a 5′ flanking region 210 comprising a first restriction enzyme site for Bsm1, in order to create a two nucleotide “GG” overhang at the first end of the precursor molecules 230 after digestion with Bsm1 to facilitate ligation into head-to-tail concatemers.

The 5′ flanking region 210 of the capture probe precursors in this Example had the following sequence: 5′ CGCGAATGCC 3′ (SEQ ID NO:2) to provide a first Bsm1 site.

Design of the 3′ Flanking Region 220 of the Capture Probe Precursor 230:

The double-stranded AKT1 capture probe precursors 230 were designed to include a 3′ flanking region 220 comprising a second restriction enzyme site for Bsm1, in order to create a two nucleotide “CC” overhang at the second end of the precursor 230 molecule after digestion with Bsm1 to facilitate ligation into head-to-tail concatemers.

The 3′ flanking region was designed to also include a third restriction enzyme recognition sequence to precisely cleave off the 3′ flanking region after amplification, and also leave 5′ terminal phosphates on the complementary strands (e.g., Psi1 or HindIII).

Using the general design principles described above, several series of oligonucleotides were synthesized as follows:

“A” Series: a control set of 64 AKT capture probes that were directly synthesized (not amplified) having a total length of 69 nucleotides.

“B” Series: a set of 64 AKT capture probe precursor oligonucleotides for amplification, but which did not impose the head to tail ligation characteristics (later abandoned, as discussed below in the results section).

“C” Series: a set of 64 AKT capture probe precursor oligonucleotides for amplification, having a total length of 79 nucleotides (34 nt 204 region that binds to the reverse primer 150, with the same 5′ flanking region 210 and target specific region 202 as D series). Note: this series was more expensive to synthesize because they required 0.2 micromolar synthesis, due to the longer size.

“D” Series: a set of 64 AKT capture probe precursor oligonucleotides for amplification, having a total length of 60 nucleotides, including a 15 nt 204 region that binds to the reverse primer 150, with a 10 nucleotide 5′ flanking region 210 and a 35 nucleotide target-specific region 202. Note: this series was much less expensive because it was synthesized on a 50 nmole scale, due to shorter size, which was then extended with the reverse primer and Klenow fill-in reaction.

As described above, the C and D series oligos were designed such that after the fill-in reaction with the common reverse primer 150, a pair of asymmetric Bsm1 sites were created on the double-stranded precursor molecule 230, such that following digestion with Bsm1, a two nucleotide “CC” overhang is present on the top strand of each double-stranded oligonucleotide and a two nucleotide “GG” overhang is present on the bottom strand of each double-stranded oligonucleotide, thus creating a situation in which only head-to-tail ligation events are allowed. Moreover, ligation recreates the Bsm1 site for downstream processing after amplification. As described in detail below, the Bsm1 digested amplification products were then treated with Antarctic phosphatase to dephosphorylate the 5′ end of the capture strand while leaving an exposed 5′ phosphate on the complement strand to allow for degradation by treatment with lambda exonuclease, to generate the desired single-stranded capture probes.

For the “D” Series oligos each capture probe precursor 100 was synthesized as shown below in TABLE 1, with the 5′ regions 210 and the 3′ regions 220 underlined.

TABLE 1 The D Series probes for AKT (NM_005163) Distance SEQ from exon ID Exon 5′ edge Strand Probe Sequence NO: 1 −35 − CGCGAATGCCGGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCTACG 3 CGTGGCGGATGT 1 0 + CGCGAATGCCATGAGCGACGTGGCTATTGTGAAGGAGGGTTGGCTACG 4 CGTGGCGGATGT 1 35 − CGCGAATGCCCCAGCCCTGGCAGCGGGTACTAACCTCGTTTGTGCACG 5 CGTGGCGGATGT 1 70 + CGCGAATGCCGCCTGGGGAGGGAGAGATGGGGGTAGTAGCCCCAGACG 6 CGTGGCGGATGT 2 −35 − CGCGAATGCCCTACAGACGTGCGGGTGGTGAGAGCCACGCACACTACG 7 CGTGGCGGATGT 2 0 + CGCGAATGCCGGGAGTACATCAAGACCTGGCGGCCACGCTACTTCACG 8 CGTGGCGGATGT 2 35 − CGCGAATGCCTTGTAGCCAATGAAGGTGCCATCATTCTTGAGGAGACG 9 CGTGGCGGATGT 2 70 + CGCGAATGCCGGAGCGGCCGCAGGATGTGGACCAACGTGAGGCTCACG 10 CGTGGCGGATGT 2 105 − CGCGAATGCCGGGATACTTACGCGCCACAGAGAAGTTGTTGAGGGACG 11 CGTGGCGGATGT 2 140 + CGCGAATGCCCTTGGCCTCTCGGGATTCAGATTTGGGGGGTTGGCACG 12 CGTGGCGGATGT 3 −35 − CGCGAATGCCCTGCGGGCAGGCAGAGCCTCTGTCTGCGTGCATCCACG 13 CGTGGCGGATGT 3 0 + CGCGAATGCCAGTGCCAGCTGATGAAGACGGAGCGGCCCCGGCCCACG 14 CGTGGCGGATGT 3 35 − CGCGAATGCCGTGGTCCACTGCAGGCAGCGGATGATGAAGGTGTTACG 15 CGTGGCGGATGT 3 70 + CGCGAATGCCTGTCATCGAACGCACCTTCCATGTGGAGACTCCTGACG 16 CGTGGCGGATGT 3 105 − CGCGAATGCCCCTGGCCTGGCCGCCACAGCCCACGTACCGCTCCTACG 17 CGTGGCGGATGT 4 −35 − CGCGAATGCCCTGCAGGAGGTCAGGTGAGGCTGCAGGCCTGTACCACG 18 CGTGGCGGATGT 4 0 + CGCGAATGCCGGAGGAGTGGACAACCGCCATCCAGACTGTGGCTGACG 19 CGTGGCGGATGT 4 35 − CGCGAATGCCGTCCATCTCCTCCTCCTCCTGCTTCTTGAGGCCGTACG 20 CGTGGCGGATGT 4 70 + CGCGAATGCCTTCCGGTCGGGCTCACCCAGTGACAACTCAGGGGCACG 21 CGTGGCGGATGT 4 105 − CGCGAATGCCGCTTGGGCTTGGCCAGGGACACCTCCATCTCTTCAACG 22 CGTGGCGGATGT 4 140 + CGCGAATGCCACCGCGTGGTGAGGCCTGTCCCCACTTCTGCCTGTACG 23 CGTGGCGGATGT 5 −35 − CGCGAATGCCCTATGGGCAGGCACCAGGGTCAGCAAGCGGCGCTGACG 24 CGTGGCGGATGT 5 0 + CGCGAATGCCACCATGAACGAGTTTGAGTACCTGAAGCTGCTGGGACG 25 CGTGGCGGATGT 5 35 − CGCGAATGCCCCTTCACCAGGATCACCTTGCCGAAAGTGCCCTTGACG 26 CGTGGCGGATGT 5 70 + CGCGAATGCCAGAAGGCCACAGGCCGCTACTACGCCATGAAGATCACG 27 CGTGGCGGATGT 5 105 − CGCGAATGCCGGCCCCACCTTGGCCACGATGACTTCCTTCTTGAGACG 28 CGTGGCGGATGT 6 −35 − CGCGAATGCCCTGTAAAGCAGGGCTGGGTGAGCTGCCACCCCGCAACG 29 CGTGGCGGATGT 6 0 + CGCGAATGCCGACGAGGTGGCCCACACACTCACCGAGAACCGCGTACG 30 CGTGGCGGATGT 6 35 − CGCGAATGCCTCACTGTGAGGAAGGGGTGCCTGGAGTTCTGCAGGACG 31 CGTGGCGGATGT 6 70 + CGCGAATGCCGTGGGAGCCCAGATGGGGCTGAAGGGCTGGGGCCAACG 32 CGTGGCGGATGT 7 −35 − CGCGAATGCCCTGCAAGGAAGGGGAGCTGGAACTGCGGCCCCACAACG 33 CGTGGCGGATGT 7 0 + CGCGAATGCCGCCCTGAAGTACTCTTTCCAGACCCACGACCGCCTACG 34 CGTGGCGGATGT 7 35 − CGCGAATGCCCCTCGCCCCCGTTGGCGTACTCCATGACAAAGCAGACG 35 CGTGGCGGATGT 7 70 + CGCGAATGCCTAGGGGCTGGGGCTGCGGGGGATGGACTTCGCGGCACG 36 CGTGGCGGATGT 8 −35 − CGCGAATGCCCTGCGGGAGGCGCAACCTGAGGCACAGCCGTGGCTACG 37 CGTGGCGGATGT 8 0 + CGCGAATGCCCTGTTCTTCCACCTGTCCCGGGAGCGTGTGTTCTCACG 38 CGTGGCGGATGT 8 35 − CGCGAATGCCCAATCTCAGCGCCATAGAAGCGGGCCCGGTCCTCGACG 39 CGTGGCGGATGT 8 70 + CGCGAATGCCTGTCAGCCCTGGACTACCTGCACTCGGAGAAGAACACG 40 CGTGGCGGATGT 8 105 − CGCGAATGCCGCCCGCCAGCGCACCTTGAGGTCCCGGTACACCACACG 41 CGTGGCGGATGT 9 −35 − CGCGAATGCCCTAGGGGAAAGGTGGCCTCAGGTCAGTGCCGCCAGACG 42 CGTGGCGGATGT 9 0 + CGCGAATGCCCTGGAGAACCTCATGCTGGACAAGGACGGGCACATACG 43 CGTGGCGGATGT 9 35 − CGCGAATGCCTCCCCTCCTTGCACAGCCCGAAGTCTGTGATCTTAACG 44 CGTGGCGGATGT 9 70 + CGCGAATGCCTCAAGGACGGTGCCACCATGAAGACCTTTTGCGGCACG 45 CGTGGCGGATGT 9 105 − CGCGAATGCCGGGGCGCACACCTCGGGGGCCAGGTACTCAGGTGTACG 46 CGTGGCGGATGT 10 −35 − CGCGAATGCCCTGCACGGGTGGCAGATGGGCAGGACTCGGCATCAACG 47 CGTGGCGGATGT 10 0 + CGCGAATGCCGTGCTGGAGGACAATGACTACGGCCGTGCAGTGGAACG 48 CGTGGCGGATGT 10 35 − CGCGAATGCCTCATCTCGTACATGACCACGCCCAGCCCCCACCAGACG 49 CGTGGCGGATGT 10 70 + CGCGAATGCCTGTGCGGTCGCCTGCCCTTCTACAACCAGGACCATACG 50 CGTGGCGGATGT 10 105 − CGCGAATGCCATCTCCTCCATGAGGATGAGCTCAAAAAGCTTCTCACG 51 CGTGGCGGATGT 10 140 + CGCGAATGCCCCGCTTCCCGCGCACGCTTGGTCCCGAGGCCAAGTACG 52 CGTGGCGGATGT 10 175 − CGCGAATGCCCTTGGGGTCCTTCTTGAGCAGCCCTGAAAGCAAGGACG 53 CGTGGCGGATGT 10 210 + CGCGAATGCCCAGAGGTGAGGGCCGCCCATCCCAGCTACAGGCTAACG 54 CGTGGCGGATGT 11 −35 − CGCGAATGCCCTGCAGGCAGGAAACAAGGCCACAGTGTCGGTACCACG 55 CGTGGCGGATGT 11 0 + CGCGAATGCCGCTTGGCGGGGGCTCCGAGGACGCCAAGGAGATCAACG 56 CGTGGCGGATGT 11 35 − CGCGAATGCCCTGCCACACGATACCGGCAAAGAAGCGATGCTGCAACG 57 CGTGGCGGATGT 11 70 + CGCGAATGCCCACGTGTACGAGAAGAAGGTGCGGCTGCTCCCCGCACG 58 CGTGGCGGATGT 12 −35 − CGCGAATGCCCTGCAGAGGTGGGCAGACGGGACAGTCATGAGCTTACG 59 CGTGGCGGATGT 12 0 + CGCGAATGCCCTCAGCCCACCCTTCAAGCCCCAGGTCACGTCGGAACG 60 CGTGGCGGATGT 12 35 − CGCGAATGCCCCGTGAACTCCTCATCAAAATACCTGGTGTCAGTCACG 61 CGTGGCGGATGT 12 70 + CGCGAATGCCCCCAGATGATCACCATCACACCACCTGACCAAGGTACG 62 CGTGGCGGATGT 13 −35 − CGCGAATGCCCTGTGGGTGTAGACAGCTCAGACCCCGGTGCCCCAACG 63 CGTGGCGGATGT 13 0 + CGCGAATGCCATGACAGCATGGAGTGTGTGGACAGCGAGCGCAGGACG 64 CGTGGCGGATGT 13 35 − CGCGAATGCCCCGCTGGCCGAGTAGGAGAACTGGGGGAAGTGGGGACG 65 CGTGGCGGATGT 13 70 + CGCGAATGCCCACGGCCTGAGGCGGCGGTGGACTGCGCTGGACGAACG 66 CGTGGCGGATGT

Oligo Synthesis: For this experiment, the initial population of oligonucleotides 100 were synthesized individually in solution by Operon (Huntsville, Ala.). In the future, the oligonucleotides will be synthesized on an array then cleaved. For example, synthesized and cleaved oligonucleotides are commercially available (e.g., available from LC Sciences, Houston, Tex., Agilent also manufactures “Sure-print” oligo arrays, cleaves the oligos and delivers pmol quantities of single-strand reagent).

Annealing and Fill-in to Generate Double-Stranded Capture Probe Precursors:

As shown in FIG. 4, Step A, the population of synthesized single-stranded oligonucleotide precursors 100 (free from substrate) were annealed with a common reverse primer oligonucleotide 150 and filled in with Klenow to form double-stranded capture probe precursor molecules 230 as shown in FIG. 4, Step A. The common reverse primer oligo 150 was designed to hybridize to the region 204 for binding to a capture reagent on the synthesized oligos 100, and includes the complement to the remaining portion of the region 204, as well as the complement to the 3′ flanking region 220. An advantage to the second strand fill in reaction with the reverse primer oligo 150 is the ability to change the 3′ flanking region 220 through the use of a reverse primer oligo 150 with different sequences in order to incorporate different restriction enzyme sites or other desired sequences in the 3′ flanking region 220 of the double-stranded capture probe precursor molecule 230.

Design of the Second Strand Reverse Oligonucleotide 150:

The following reverse primer oligonucleotides 150 were used in this Example:

Reverse primer #1: (Psi 1, Bsm1 3′ end) (SEQ ID NO: 67) 5′ GAGGTCGGCATTCTTATAATTGCTCGAAGGGGTCCACATC CGCCACGCGT 3′; Reverse primer #2: (HindIII, Bsm1 3′ end) (SEQ ID NO: 68) 5′GAGGTCGGCATTCAAGCTTAATTGCTCGAAGGGGTCCACA TCCGCCACGCGT 3′

Preparation of Oligo Pools and Annealing:

As shown in FIG. 4A, each oligonucleotide 100 in the pool of synthesized oligonucleotides (e.g., pool D, SEQ ID NO:3-66) was annealed to a common reverse primer 150 (SEQ ID NO:67 or SEQ ID NO:68) and filled-in with Klenow to generate a double-stranded oligonucleotide precursor 230 as follows:

Pooling of Oligonucleotides:

Each A, B, C, and D series oligos were resuspended to 100 μM, then each of the oligos in the A, B, C, and D series were pooled separately, to create an A series pool, a B series pool, a C series pool, and a D series pool at 100 μM, each pool containing a mixture of 64 different oligonucleotides. The DNA concentration of the oligo pool (100 μm of 70 to 80mer=2 μg/μl) was confirmed by agarose gel electrophoresis by diluting the pool 50-fold to 40 ng/μl, and loading 2.5 μl and 5 μl.

The common reverse primer 150 (#2: SEQ ID NO:68) for second strand synthesis was resuspended to 100 μM.

Annealing:

The following reagents were combined to give a 1 μM solution in each primer (40 ng/μl combined):

10 μl of 100 μM primer pool C or D (SEQ ID NO:3 to SEQ ID NO:66)

10 μl of 100 μM reverse primer #2 (SEQ ID NO:68)

100 μl New England Biolabs Buffer #4

880 μl H₂0

1000 μl total

A 100 μl aliquot of the above mixture was heated to 95° C., then cooled down as shown below to room temperature:

95° C., 2 minutes

80° C., 1 minute

75° C., 1 minute

70° C., 1 minute

65° C., 1 minute

60° C., 1 minute

55° C., 1 minute

Room temperature, hold.

A 2.5 μl and 5.0 μl aliquot of the annealed mixture was checked on an agarose gel.

Fill-in Reaction:

The annealed mixture was then treated with Klenow to fill in both strands, thus generating a population of blunt ended, double-stranded precursor molecules 230 as shown in FIG. 4, Step B, each having a target-specific region 202 and a common sequence region 204 for binding to a capture reagent. It will be understood by those of skill in the art that the double-stranded precursor oligo structure 230 may also be generated by synthesis of the top and bottom strands, followed by annealing of the single strands into a double-stranded structure having the desired “GG” nucleotide overhang on the first end and the desired “CC” nucleotide overhang on the second end of the molecule to facilitate head to tail concatemerization upon ligation.

Second strand synthesis of each of the annealed oligo mixtures “C” and D″ were carried out as follows:

100 μl annealed primer mixture, described above

1 μl 10 mM dNTPs

2 μl Klenow (3′ to 5′ exo-) 5000 units/ml (M0212S), New England Biolabs, MA

Incubated at 37° C. for 30 minutes, 75° C. for 20 minutes. A 3 μl or 6 μl aliquot of each pool was checked on an agarose gel.

Exemplary double-stranded capture probe precursors 230 are provided below based on the “D” series oligo for exon 1 (−35) (SEQ ID NO:3), provided in Table 1:

The top strand of the filled-in double stranded product (SEQ ID NO:3 annealed to Reverse primer #1 SEQ ID NO:67)is:

(3′ flanking region: Psi1/Bsm1) (SEQ ID NO: 69) 5′CGCGAATGCCGGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCTAC GCGTGGCGGATGTGGACCCCTTCGAGCAATTATAAGAATGCCGACC TC 3′

The bottom strand of the filled-in double-stranded product (SEQ ID NO:3 annealed to Reverse Primer #1 SEQ ID NO:67) is:

(3′ flanking region: Psi1/Bsm1) (SEQ ID NO: 70) 5′GAGGTCGGCATTCTTATAATTGCTCGAAGGGGTCCACATCCGCCACG CGTAGGAGCGCGTGAGCGTCGCGGGAGCCTCGGGCACCGGCATTCG CG-3′

The top strand of the filled-in double-stranded product (SEQ ID NO:3 annealed to Reverse Primer #2 SEQ ID NO:68)is:

(3′ flanking region: HindIII/Bsm1) (SEQ ID NO: 71) 5′CGCGAATGCCGGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCTAC GCGTGGCGGATGTGGACCCCTTCGAGCAATTAAGCTTGAATGCCGACC TC-3′

The bottom strand of the filled-in double stranded product shown is:

(3′ flanking region: HindIII/Bsm1) (SEQ ID NO: 72) 5′GAGGTCGGCATTCAAGCTTAATTGCTCGAAGGGGTCCACATCCGCCA CGCGTAGGAGCGCGTGAGCGTCGCGGGAGCCTCGGGCACCGGCATTCG CG-3′ Digestion with Bsm1 to Generate Ligation Substrates:

As shown in FIG. 4, Step B, the filled-in double-stranded capture probe precursors 230 were then digested with Bsm1 to generate ligation substrates as follows:

2 μl of Bsm1 (10,000 units/ml, R0134S, New England Biolabs) was added to each of the 100 μl heat inactivated fill-in reactions and incubated at 65° C. for 1 hour. 3 μl and 6 μl of each pool was checked on an agarose gel. The Bsm1 digests were then purified over a QIAQUICK column (Qiagen), eluted and quantified by nanodrop.

Resulting exemplary double-stranded oligonucleotide structures after Bsm1 digestion, as illustrated in FIG. 4, Step B:

The top strand of the Bsm1 digested double-stranded oligo (SEQ ID NO:69/SEQ ID NO:70) is:

(3′ flanking region: Psi1) (SEQ ID NO: 73) 5′GGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCTACGCGTGGCGGA TGTGGACCCCTTCGAGCAATTATAAGAATGCC 3′

The bottom strand of the Bsm1 digested double-stranded oligo (SEQ ID NO:69/SEQ ID NO:70) is:

(3′ flanking region: Psi1). (SEQ ID NO: 74) 5′CATTCTTATAATTGCTCGAAGGGGTCCACATCCGCCACGCGTAGGAG CGCGTGAGCGTCGCGGGAGCCTCGGGCACCGG3′

The top strand of the Bsm1 digested double-stranded oligo (SEQ ID NO:71/SEQ ID NO:72) is:

(3′ flanking region: HindIII) (SEQ ID NO: 75) 5′GGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCTACGCGTGGCGGA TGTGGACCCCTTCGAGCAATTAAGCTTGAATGCC 3′

The bottom strand of the Bsm1 digested double-stranded oligo (SEQ ID NO:71/SEQ ID NO:72) is:

(3′ flanking region: HindIII) (SEQ ID NO: 76) 5′CATTCAAGCTTAATTGCTCGAAGGGGTCCACATCCGCCACGCGTAGG AGCGCGTGAGCGTCGCGGGAGCCTCGGGCACCGG3′

Ligation of Bsm1 Digested Precursors to Form Head-to-Tail Concatemers:

As shown in FIG. 4, Step C, the Bsm-1 digested products 230 were then used as ligation substrates to generate a series of head-to-tail concatemers 240 for use as amplification templates.

Ligation was carried out as follows:

The following reagents were combined for each pool “C” and “D”:

50 μl 2× quick ligase buffer (New England Biolabs)

11 μl of 48 ng/μl Bsm1 digested pool C or D

34 μl H₂O

5 μl ligase (New England Biolabs)

100 μl total

Incubated at room temperature for ≧10 minutes. A no DNA control (no template) was also prepared.

As illustrated in FIG. 4, Step C, it is noted that the ligation reaction naturally drives towards the formation of circularized templates due to the fact that in very dilute solutions of free ends, the probability of self-ligation (circularization) becomes higher than the probability of finding a separate free end.

Amplification of Head-to-Tail Concatemers:

As shown in FIG. 4, Step D, circularized concatenated DNA was then amplified by greater than 1000 fold (i.e., 10,000 fold to 20,000 fold) to form amplified concatemers 260 by using a random amplification primer 250 with the strand displacement polymerase technology found in the TEMPLIPHI isothermal amplification kit (GE Life Sciences, Piscataway, N.J.).

The random amplification primer 250 used in this Example was a random 7-mer amplification primer with an additional two nitroindole residues at the 5′ end and a phosphorothioate linkage at the 3′ end: 5′[nitroindole]2-[N]6-(phosphothioate)-N (SEQ ID NO:77, wherein the “N” at positions 1-7 may be A, G, C or T), as described by Lage et al., Genome Res 13:294-307 (2003), incorporated herein by reference.

Isothermal Amplification:

Four reaction mixtures of “C” pool and “D” pool ligations were prepared as follows, along with 2 reaction mixtures of a “no template” control:

25 μl of 100 μM amplification primer [5-nitroindole]2-[N]6-(phosphothioate)-N (SEQ ID NO:77)

10 μl ligated template “C” or “D” pool (or no template control)

5 μl 10× phi29 buffer (New England Biolabs)

10 μl H₂0

50 μl total

The above reagents were mixed and incubated at 95° C. for 3 minutes, then cooled to room temperature, then 50 μl of the following enzyme premix was added:

Enzyme Premix:

25 μl of 100 μM amplification primer (SEQ ID NO:77)

10 μl H₂0

5 μl 10× phi29 buffer (NEB)

4 μl 10 mM dNTPs

2 μl 100 mM DTT

2 μl 10 mg/ml BSA (NEB)

2.5 μl phi29 polymerase (NEB)

50 μl total volume of enzyme premix

The 50 μl annealed mixture was combined with the 50 μl enzyme premix, then incubated at 30° C. for 12 hours. The polymerase was inactivated by incubation at 65° C. for 10 minutes, then cooled to 4° C. The mixture was briefly centrifuged to pellet the protein and the supernatant was transferred to a fresh tube.

The isothermal amplification reactions were then ethanol precipitated by combining the four “C” reactions and the four “D” reactions (separately), then adding 600 μl TEzero, split into two tubes of 500 μl each, then adding 120 μl 3M NaOAc at pH 5.2 to each tube, then 1200 μl ethanol. The reactions were centrifuged for 10 minutes at 12K RPM and the pellets were resuspended in 880 μl TEzero. The amount of DNA recovered was quantitated. The yield was determined to be 65 to 79 ng/μl for all four tubes, therefore, each 100 μl isothermal amplification reaction produced 28.5 to 30 μg of DNA, which was an unexpectedly high yield. It is noted that the isothermal amplification reaction carried out with 50 μM of the random amplification primer [5-nitroindole]2-[N]6-(phosphothioate)-N (SEQ ID NO:77) in combination with 400 nM dNTPs and DTT as described above provided reaction conditions that yielded a significantly higher amount of amplification product than was obtained from an amplification reaction with the same templates using the reagents from a commercially available kit (GE Healthcare Life Sciences).

It is noted that an initial attempt was made to simply ligate dsDNA blunt-end oligonucleotide probes together followed by amplification (Series “B” pool). However, it was determined that this initial approach was not suitable for uniformly amplifying a population of probes for solution-based capture because the oligonucleotides were ligated in random head to tail, head to head and tail to tail orientation. In the head to head and tail to tail orientations some common tail sequences hybridized together, thereby creating a snap-back stem in ssDNA. These ssDNA stem regions were poisonous to polymerases, resulting in under-representation of amplified products.

Digestion of Amplified Concatenated Strands into Monomer Double-Stranded Capture Probe Precursors:

As shown in FIG. 4, Step E, the amplified concatenated strands 260 were then cleaved into monomer double-stranded capture precursors 270 with Bsm1 and 5′ terminal phosphates were removed with Antarctic Phosphatase, as follows:

Digestion with Bsm1 after Isothermal Amplification:

55 μg from each amplification reaction was digested with 20 μl Bsm1 in a total volume of 1 ml in 1×NEB Buffer #4 buffer at 65° C. for two hours. After the two hour digestion, an aliquot of each digest was checked on an agarose gel. The majority of the “C” and “D” digested pool showed the expected 90 bp product, with a small amount of 130 bp product.

Phosphatase Treatment:

110 μl 10× Antarctic Phosphatase buffer (NEB) was added to the Bsm1 digested pool C and D. 50 μl Antarctic phosphatase was added (NEB), and incubated for one hour at 37° C. then 65° C. for 15 minutes. An aliquot of 10 μl was taken out of each sample, then the samples were ethanol precipitated.

After ethanol precipitation, each sample was split into four tubes of 250 μl each to which was added: 250 μl TEzero, 120 μl of 3M NaOAc pH5.2, 2 μl glycol and mixed. 1200 μl ethanol was added per tube, mixed, precipitated and centrifuged at 12K RPM, 10 minutes.

Digestion With Psi1 or HindIII to Liberate the Precise 3′ Ends of the Capture Probes:

As shown in FIG. 4, Step F, monomeric capture precursors and the precise 3′ ends of capture probes were then liberated by digestion with Psi1 or HindIII, which also leaves 5′ terminal phosphates on the complementary strands.

After the phosphatase treatment as described above, the pellets were then resuspended in 960 μl of 1×NEB #4 buffer and 40 μl PsiI, then digested for 2 hours at 37° C. The digests were then ethanol precipitated.

Selective Removal of the Complementary Strand of the Monomer Capture Probe Precursors To Produce a Population of Single-Stranded Capture Probes for Solution Based Capture:

As shown in FIG. 4, Step G, a population of single-strand capture probes 200 (i.e., a library), each probe 200 containing a distinct target specific region 202 and a common capture reagent binding region 204, were generated by digesting the double-stranded monomer capture probe precursors 270 with Lambda exonuclease. Lambda exonuclease specifically degrades dsDNA by attacking at 5′ phosphate sites and selectively digests away the non-capture complementary strand, thereby converting the dsDNA into ssDNA suitable for use as capture probes.

Lambda Exonuclease Digestion:

An enzyme titration was first run with lambda exonuclease starting at a concentration of 1 μl/10 μl and diluted down in 2-fold steps. Each reaction contained 10 μg substrate (digested with Bsm1, alkaline phosphatase and Psi1) in 100 μl and were digested at 37° C. for 10 minutes, 75° C. for 10 minutes, then cooled to 4° C. The reactions were run on an agarose gel and the conditions of 10 μg substrate in 100 μl with 5 μl exonuclease for 10 minutes at 37° C. was used for subsequent digestions of the pool C and pool D substrate.

Scaled Up Reaction:

For capture series “C”, 90 μl of 89 ng/μl dsDNA precursor was digested with 5 μl lambda exonuclease in 100 μl 1× lambda exonuclease buffer (NEB).

For capture series “D”, 100 μl of 208 ng/μl dsDNA precursor was digested with 5 μl lambda exonuclease in 200 μl 1× lambda exonuclease buffer (NEB).

The following is an exemplary structure of a final single-stranded capture probe 200 resulting from the capture probe precursor SEQ ID NO:69/70 or SEQ ID NO:71/72 after processing with lambda exonuclease:

(SEQ ID NO: 78) 5′GGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCTACGCGTGGCGGA TGTGGACCCCTTCGAGCAATTA3′ 3′

The capture probe SEQ ID NO:78 comprises a 5′ region 202 that hybridizes to the −35 exon 1 of the AKT gene, and a 3′ region 204 (underlined) that hybridizes to a universal biotinylated oligonucleotide 300.

The universal capture hybridizing region 204 of capture probe SEQ ID NO:78 is:

(SEQ ID NO: 80) 5′ ACGCGTGGCGGATGTGGACCCCTTCGAGCAATTA 3′

Discussion:

The isothermal amplification method described in this Example converted 2.5 ng (0.1 μmol) of starting material into 50 μg (2000 μmol) of raw, unprocessed double-stranded material, which is a 20,000-fold level of amplification. In comparison, the Agilent custom library array platform provides 250 ng (10 μmol) of cleaved oligonucleotide. Processing of the double-stranded DNA precursor capture probe 230 to single stranded capture probes 200 resulted in approximately 15 μg (1200 μmol) of single-stranded capture probe 200, which is a 60% yield from the starting amplified unprocessed double-stranded material 230. In addition to high yield of amplified products, the use of concatemers 240 as templates for amplification provides an equal distribution of amplified monomer products 270, resulting in an equal distribution of processed capture probes 200. The lambda exonuclease digested material (ssDNA) 200 was successfully used as a capture probe library for solution based capture of the AKT exons 1-13, as described in Example 2.

Example 2

This Example describes solution-based capture using a pool of capture probes 200 generated as described in Example 1, each capture probe 200 comprising a target specific region 202 specific for binding to one of the 13 exons of AKT (NM_(—)005163) and a common region 204 that hybridizes to a universal biotinylated adaptor oligo 300.

Rationale:

As shown in FIG. 2, target gene enrichment of a genomic library may be achieved by indirect capture using a pool of chimeric capture ssDNA probes 200 with a first region 202 that hybridizes to a target nucleic acid sequence 10 and a second region 204 that hybridizes to a universal biotinylated oligo 300, mixing the chimeric oligo 200, the universal biotinylated oligo 300 and the sample containing the target nucleic acid sequence 10 under hybridizing conditions to form a tri-molecular complex (i.e., 10/200/300), and using magnetic beads 400 coated with streptavidin 410 to bind to the biotinylated region 310 of the universal oligo 300 and pull out the target sequences 10 bound in the complex to the chimeric capture probes 200, using a magnet 500.

While indirect capture is described in this Example, it will be understood by those of skill in the art that solution based capture may also be accomplished through the use of ssDNA probes 200 are directly labeled. For example, the probes 200 could be directly labeled by adding a biotin, deoxygenin, fluorescein, and the like (through the use of commercially available kits), followed by the use of antibody coated beads for purification.

Methods:

ssDNA capture probes were generated as described in Example 1. For capture series “C” and “D,” the concentration of capture probe after the lambda exonuclease digestion was approximately 40 ng/μl to 50 ng/μl.

The following universal 5′ biotinylated oligo (capture reagent 300) was used in this Example:

(SEQ ID NO: 81) 5′ [BioTEG] TAATTGCTCGAAGGGGTCCACATCCGCCACGCGT 3′

As described in Example 1, a library of 64 ssDNA chimeric capture oligos 200 were generated that each target one of the 13 exons of AKT1 that each have a first 5′ region 202 with the identical sequence to the oligos shown above in TABLE 1, and a second 3′ region 204 consisting of the following additional sequence that hybridizes to the universal biotinylated oligo 300:

(SEQ ID NO: 80) 5′ ACGCGTGGCGGATGTGGACCCCTTCGAGCAATTA 3′.

Capture Mixture:

A master mix was prepared by combining 62.5 μl of 80 ng/μl of a genomic DNA library containing an average insert size of 100 bp flanked by a first and second primer binding site, 10 μl of 1 μM universal biotinylated oligo (SEQ ID NO:81), 125 μl 2× binding buffer (20 mM Tris pH 7.6, 0.2 mM EDTA, 2M NaCl).

The master mix was then combined (separately) with the following:

10 μl of 1 μM Maxwell 139 AKT1 set, a set of 28 directly synthesized capture oligonucleotides specific for AKT exons 1-13 (low density coverage).

10 μl of 1 μM “A” series high density AKT1 control set (64 oligo pool) that was directly synthesized (not amplified). Each capture probe was synthesized to contain only the target specific portion 202 and the universal oligo hybridizing portion 204, without the flanking sequences. For example, the probe for AKT exon 1 (−35) was identical to the final processed amplified probe for AKT exon 1 (−35) (SEQ ID NO:78).

10 μl of processed “C” Series Capture probes (in duplicate): a set of 64 AKT capture probes generated using the amplification method described in Example 1.

10 μl of processed “D” Series Capture probes: a set of 64 AKT capture probes generated using the amplification method described in Example 1.

20 μl of processed “D” Series Capture probes, as described for Sample #4 above.

Each reaction was brought to a total volume of 250 μl, mixed, and annealed as follows:

95° C. for 5 minutes

80° C. for 15 minutes

75° C. for 15 minutes

70° C. for 15 minutes

65° C. for 15 minutes

60° C. for 15 minutes

55° C. for 15 minutes

Room Temperature.

Capture Reagents:

Washed streptavidin-coated magnetic beads were prepared by combining 66 μl beads (MyOne streptavidin-coated beads, InVitrogen) 500 μl 2× binding buffer and 440 μl water.

Capture:

Each of the annealed 250 μl mixtures were combined with 10 μl of washed beads in a total volume of 1 ml (10 mM Tris, pH 7.6, 0.1 mM EDTA, 1 M NaCl, 0.1% Triton X100) and incubated with mixing for 15 minutes. The beads were then washed four times, 5 minutes each, with 1 ml of TEzero wash buffer (10 mM Tris pH 7.6, 0.1 mM EDTA) containing 25% formamide.

Elution:

The DNA bound to the beads was eluted with two 25 μl aliquots of water by incubation at 95° C. for 1 minute, pulling over the beads, and removing the eluate, for a total eluate volume of 50 μl

Amplification of Eluate: PCR Reaction Mixture (Each Sample Performed in Duplicate)

10 μl template (eluate from enriched fragment library)

30 μl H₂O

20 μl 5×PCR buffer (supplied by manufacturer with the EXPAND^(plus)® kit, Roche)

10 μl 25 mM MgCl₂

10 μl Forward PCR primer (5′-AATGATACGGCGACCACCGA-3′ (SEQ ID NO: 82)) 10 μl Reverse PCR primer (5′-CAAGCAGAAGACGGCATACG-3′ (SEQ ID NO: 83))

5 μl 10 mM dNTPs

5 μl DMSO

1 μl Expand^(PLUS)® polymerase (Roche)

100 μl total volume

PCR Cycling Conditions: 1 Cycle:

95° C. for 2 minutes

10 Cycles:

95° C. for 30 sec

60° C. for 30 sec

72° C. for 1 minute

10 Cycles:

95° C. for 30 sec

60° C. for 30 sec

72° C. for 1 minute plus 10 sec/cycle

1 Cycle:

72° C. for 5 minutes

4° C. hold

The PCR products were purified over a QIAQUICK column, quantified and diluted to 1 ng/μl for subsequent quantitative PCR (qPCR) (Taqman) analysis.

Quantitative PCR Analysis:

The PCR products generated as described above (1 ng/μl), no template control and genomic DNA control (10 ng/μl) were used as templates in Taqman assays directed against coding exons 1-13 of AKT1. Negative controls ANKHD, PIK3CA and TP53 were also included in this assay, which should not be captured as target sequences with the AKT specific probe pools.

Results:

The results of the qPCR analysis were analyzed in two ways. First, the fold-enrichment for each solution-based capture over genomic DNA was calculated, as shown in TABLE 2.

TABLE 2 Fold Enrichment relative to genomic DNA of solution- based capture using various capture probe pools Series Series Targeted Maxwell Series C C Series Series AKT 139 A (set #1) (set #2) D D exon (10 μl) (10 μl) (10 μl) (10 μl) (10 μl) (20 μl) exon 1 1970 1158 3471 3376 3802 6370 exon 2 1338 931 2790 2895 3662 6450 exon 3 1131 989 2480 2568 3452 6044 exon 4 1579 1072 2508 2507 4189 7122 exon 5 1460 1189 3408 3840 5184 8878 exon 6 1641 1002 3083 3461 4048 5586 exon 7 1435 1048 3578 3660 4642 6167 exon 8 1331 1112 2408 2212 3780 7418 exon 9 1242 1194 3495 3817 4746 8526 exon 10 1528 1227 3146 3176 4675 9464 exon 11 1314 1103 2592 2638 3741 6904 exon 12 1478 960 1964 2511 3739 6862 exon 13 1662 1107 754 648 4236 6504 ANKHD 1 1 1 0 0 1 PIK3CA 0 0 0 0 0 0 TP53 0 1 1 0 0 1

Second, the standard deviation of the exon-to-exon capture efficiency was calculated as a percent of the overall fold-enrichment, as shown below in TABLE 3. This latter number provides a measure of the uniformity of each capture reaction.

TABLE 3 Standard deviation of the exon-to-exon capture efficiency Series Series Max Series C C Series Series 139 A (set #1) (set #2) D D (10 μl) (10 μl) (10 μl) (10 μl) (10 μl) (20 μl) Average fold 1470 1084 2745 2870 4146 7100 enrichment for AKT exons 1-13 standard 216 94 782 863 523 1175 deviation of exon-to-exon capture efficiency Percent 15% 9% 28% 30% 13% 17% deviation, expressed as a percentage of the fold- enrichment

FIG. 6 graphically illustrates the fold of enrichment of the target AKT exons 1 to 13 (shown on the x-axis) relative to genomic DNA of solution-based capture using 100 of each of the following probe pools: the “Maxwell 139” capture probe pool (low density, directly synthesized oligonucleotides), the “Series A” capture probe pool (high density, directly synthesized oligonucleotides) and the “Series D” capture probe pool (high density, generated via amplification of head-to-tail concatemers). As shown in FIG. 6, the fold enrichment using the Series D capture probe pool was at least as good as the fold enrichment using the Series A capture probe pool.

Discussion:

The “A” series oligos were directly synthesized 69 mers (non-amplified) containing the identical sequence as the probe sequences generated after processing amplified sequences. The “C” series oligos were initially synthesized as 79mers, annealed to a reverse primer, filled-in, digested with Bsm1, ligated into concatemers, amplified, digested back to monomers, and lambda-exo treated to generate ssDNA probes. The “D” series oligos were initially synthesized as 60mers (less overlap with reverse primer than the “C” series), annealed to a reverse primer, filled-in, digested with Bsm1, ligated into concatemers, amplified, digested back to monomers and lambda-exo treated to generate ssDNA probes. It is important to note that the “D” series oligos are the least expensive to generate due to the fact that they can be made on a 50 nmole synthesis scale due to their smaller size. As described above in Example 1, the amplification of the capture probe pool “C” or “D” was observed to be in the range of 10,000 to 20,000 fold amplification, thereby facilitating the cost-effective use of solution based capture for target enrichment in a variety of applications.

It is noted that the absolute magnitude of the fold-enrichment shown in TABLE 2 may be exaggerated because the critical stoichiometry between the capture oligonucleotides and the common, biotinylated adaptor capture reagent oligonucleotide was not optimized in this experiment. However, the key metric is that the standard deviation of exon-to-exon capture efficiency, expressed as a percentage of the fold-enrichment, as shown in TABLE 3 and FIG. 6, was essentially identical for the synthetically generated capture probes (Series A=9%) versus the capture probes generated using the amplification methods described in Example 1 (Series D=13%).

Agarose gel analysis of the capture probes generated by the amplification methods described herein showed that they formed bimolecular complexes during solution based capture that were indistinguishable from the biomolecular complexes formed with directly synthesized capture probes. Importantly, as demonstrated in TABLES 2 and 3 and FIG. 6, the solution based capture of the 13 exons of the AKT gene with the capture reagent generated by amplification worked at least as well as control reactions performed with directly synthesized capture probes. This conclusion is based on the metrics of fold-enrichment and on the standard deviation of exon-to-exon enrichment as a percentage of the overall fold-enrichment.

Therefore, this Example demonstrates that the capture probes generated from amplified head-tail concatemers may be successfully used for solution based capture, and provide an advantage in targeted resequencing by reducing the cost of resequencing while increasing the feasibility of profiling applications that are dependent on complex oligonucleotide libraries.

Example 3

This Example describes a method for designing and uniformly amplifying a library of target capture probes designed to capture the entire collection of exons that include protein coding regions from a human genomic DNA library.

Rationale:

As described in Examples 1 and 2, a method for uniformly amplifying a library of synthesized capture oligonucleotides for use in solution based capture methods can be applied to capture all the exons of a gene, such as AKT. This Example demonstrates that the methods described herein can be applied on a very large scale, in order to generate a library of capture probes that capture the entire collection of genomic segments that include all protein coding regions from a human genomic library.

Methods:

Design of Capture Probe Oligonucleotide Precursors for Amplification and Processing into a Library of Capture Probes:

As shown in FIG. 4, step A, the general structure of the double-stranded capture probe precursor includes a 5′ flanking region 210 comprising a first restriction enzyme site for creating a first nucleotide overhang for ligation (e.g., Bsm1), a capture probe region 200 comprising a target specific hybridizing region 202 and a universal capture oligo hybridizing region 204, and a 3′ flanking region 220 comprising a second restriction enzyme site (e.g., Bsm1) for creating a second nucleotide overhang for ligation, and further comprising a third restriction enzyme site (e.g., Psi1 or HindIII), for precisely cleaving off the 3′ flanking region.

Design of the Target-Specific Region 202 of the Capture Probe 200:

In this Example, a capture probe library 200 comprising 1,148,286 distinct target-specific regions 202 was generated for high density solution based capture of the entire collection of genomic segments that include protein coding regions (exons) for 25,341 annotated human genes.

The overall design principles for designing a capture probe library 200 comprising target-specific regions 202 for capture of all the exons of 25,341 human genes were as follows. Each target-specific region 202 was 35 nucleotides in length. The target-specific region was designed such that each target exon had at least 4 or more probes (high density), and the capture probes alternated in strand orientation and were oriented in a head to tail arrangement, with oligo probes alternating with respect to hybridizing to the coding or non-coding strand of the target exon.

In this Example, the term “candidate oligonucleotide” refers to a 35mer nucleotide sequence that was analyzed for potential use as target-specific region in a capture probe, to determine whether the candidate oligonucleotide sequence met the desired criteria, as described below. The 35mer nucleotide sequences that met all of the design criteria outlined herein were chosen as the set of target-specific regions 202 and were synthesized on a microarray along with flanking sequences to generate an oligonucleotide library having 1,148,286 distinct target-specific regions.

Step 1: Obtain Input Sequence:

In order to design the target-specific sequences of the library of capture probes, the genes and transcripts of interest were first identified. In this Example, the entire list of human high quality mRNA transcripts provided in the publicly accessible NCBI database “RefSeq NM transcripts” was selected as input sequence, which was a total of 25,341 human annotated mRNA transcripts. The protein coding exons in the 25,341 input human mRNA transcripts were then identified using the publicly accessible “UCSC Genome Browser” database, accessible at http://genome.ucsc.edu/. The UCSC Genome Browser is developed and maintained by the Genome Bioinformatics Group, a cross-departmental team within the Center for Biomolecular Science and Engineering at the University of California Santa Cruz. Once the protein coding exons were identified, the genomic sequences of interest plus 100 nucleotides of adjacent intronic sequences on either side of the exons were extracted. Overlapping regions were then identified and removed from the list of sequences by using genomic coordinates. For overlapping regions that were identified, the sequence that was retained was based on the 5′ most and 3′ most genomic coordinates between all the pairs of exons in the overlapping region.

Step 2: Upfront Sequence Classification:

The list of sequences generated as described in Step 1 was then searched using the software program “repeatmasker” (publicly accessible at http://www.repeatmasker.org/) to identify, but not mask, the repeat and low complexity elements.

Step 3: Determination of Uniqueness Score of Candidate Oligonucleotide Probe Sequences:

The uniqueness of all 35mer sequences in the sequenced human genome relative to the human genome was determined as follows. First, the “UCSC Genome Browser” database was used to extract every 35mer sequence from the sequenced human genome. Second, the software algorithm “Burrows-Wheeler Alignment” (hereinafter referred to as “BWA”) (publicly available at http://maq.sourceforge.net/bwa-man.shtml) was used to align (i.e., blast) these 35mer sequences against the entire sequenced human genome. BWA is a fast light-weighted tool that aligns short sequences to a sequence database, such as the human reference genome. For each 35mer candidate oligo sequence, BWA returned an alignment score that measures the confidence in the identified location, as described in Li, H., et al., Genome Res. 18(11):1851-8 (2008), incorporated herein by reference. The alignment score provided by BWA is a-log10 p-value, ranging from 37 (unique in the genome) to 0 (multiple perfect matches). Intermediate values reflect alignments that are similar but not 100% (e.g., with one nucleotide mismatch).

Step 4: Final Selection of the Target-Specific Regions 202 for Synthesis to Generate a Library of Capture Probes 200 for Total Exon Capture of the Human Genome:

The final selection of the 1,148,286 distinct target-specific regions for synthesis in order to generate a library of capture probes 200 that hybridizes to all the exons of 25,341 annotated genes was carried out as follows.

For each target exon at least 140 nucleotides or longer, analysis for candidate oligos began at the 5′ edge of the exon. For target exons 140 nucleotides or shorter, the analysis began in the 5′ intron such that the candidate oligos were equally spaced at the exon center.

From the starting position, each candidate oligo location was then “jittered” by shifting the region of analysis by +/−4 nucleotides as follows. The nucleotide sequence of the candidate oligo closest to the desired location was first examined, and the examination of candidate oligos was carried out by shifting the region of analysis in the order: +1, −1, +2, −2, +3, −3, +4, −4. The +/−4 nucleotide range was chosen because a wider range (more “jitter”) may allow oligo dimers to form with adjacent oligos, which would be undesirable.

The best candidate oligos from each “jittered” position were then selected based on the following criteria. First, the BWA alignment score (uniqueness) of the candidate 35mer capture oligo sequences was maximized relative to the entire genome (wherein 37=unique and 0=multiple perfect matches). Second, among the candidate 35mer capture oligo sequences with the same BWA alignment score, the number of repeat/low-complexity nucleotides contained in the 35mer sequence was minimized.

The nucleic acid sequences of the 1,148,286 distinct target-specific 35mer regions were output as a text file to an oligonucleotide synthesis platform and synthesized.

In this Example, the target-specific regions were flanked by a 3′ flanking region (SEQ ID NO:1) for annealing to a common reverse primer for second strand synthesis, and by a 5′ flanking region (SEQ ID NO:2) which provides at least one processing site for ligation and amplification, resulting in a structure corresponding to the single-stranded capture probe precursor 100 as illustrated in FIG. 4, Step A, and described in Example 1. The processing of the library of capture probe precursors 100 to double-stranded precursors 230, processing of the 5′ and 3′ flanking regions to generate ligation substrates, ligation into head-to-tail concatemers, amplification and processing of the amplified head-to-tail concatemers to form monomers, and selective removal of the non-capture complement strand of the monomers to form a library of single-stranded capture probes, is carried out as described in Example 1.

TABLE 4 below provides the sequence information and scores from the selection criteria described above for a representative, randomly chosen subset of 2170 capture probe precursors from the total library of 1,148,286 capture probe precursors that were synthesized.

In particular, TABLE 4 provides the following information: Column 1: gene name; Column 2: Genbank transcript reference number; Column 3: target exon number; Column 4: the chromosome of the target exon; Column 5: the gene strand; Column 6: the distance of the capture probe from the 5′ edge of the target exon; Column 7: the strand of the capture probe; Column 8: the number of repeat nucleotides; Column 9: the BWA alignment score (wherein 37=unique and 0=multiple perfect matches); Column 10: the number of hits from the BWA alignment (1=unique); Column 11: the number of hits from BWA mismatch (0=no mismatch; 1=mismatch); Column 12: the sequence of the single-stranded precursors, each including the common 5′ flanking region (SEQ ID NO:2) and common 3′ flanking region (SEQ ID NO:1); and Column 13: the SEQ ID NO: from SEQ ID NO:84 to SEQ ID NO:2253.

As shown in TABLE 4, Column 9, a score of “1” for the “number of hits from the BWA analysis corresponds to a uniqueness score of a perfect 37, in which capture oligo sequence only recognizes the intended target.

TABLE 4 Representative subset (2170) of a total of 1,148,286 Capture Probes Designed for Exon Capture of 25,341 Human Genes Distance Gene from exon 5 Oligo # repeat BWA # BWA SEQ ID Gene Transcript Exon Chromosome strand edge strand nucleotides score BWA hit mismatch Sequence with add-ons NO: KSR2 NM_173598 15 12 − −35 + 0 37 1 0 CGCGAATGCCCCTCTGTGTAACAGGCTGTTCTCT 84 TCTCTCTGTAGACGCGTGGCGGATGT KSR2 NM_173598 15 12 − 0 − 0 37 1 0 CGCGAATGCCGATCTGCGGGGACCAGCGTGGCA 85 CTGACAGTGTGTACGCGTGGCGGATGT KSR2 NM_173598 15 12 − 35 + 0 37 1 0 CGCGAATGCCCCTCGCAGAGATCTCGGCAACTC 86 CATCAAGCACAGACGCGTGGCGGATGT KSR2 NM_173598 15 12 − 70 − 0 37 1 0 CGCGAATGCCAAGTAGGCACCAGTGCACACAGG 87 AATCCAGCCTACACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 0 + 0 37 1 0 CGCGAATGCCTCCTGGCCCAGCTGCTGCCGCTC 88 CTGCACGGCAATACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 35 − 0 37 1 0 CGCGAATGCCTGGAACTCCCGGATGATGACCTT 89 GCTCCCGTTCACACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 70 + 0 37 1 0 CGCGAATGCCGGAGCACTGCCGCCGGGGACTGC 90 TCAGCAACCACAACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 105 − 0 37 1 0 CGCGAATGCCCAGGTAGGTGGTGGAGGGGCTCC 91 GCGGGCTGCCGGACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 140 + 0 37 1 0 CGCGAATGCCCACACCCCCACCCCCAGCGAGGA 92 TGCCGCCATCCCACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 175 − 0 37 1 0 CGCGAATGCCTCTCGGAAATGAGCCGCTTGAGC 93 CGGGACTTAGAGACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 210 + 0 37 1 0 CGCGAATGCCACTCAGTGTATGAGAAGCGGCCT 94 GACTTCAGGATGACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 245 − 0 37 1 0 CGCGAATGCCAAGCTCTGTAGCACCTGCGGGTG 95 CACGTACCAGCAACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 280 + 0 37 1 0 CGCGAATGCCCCAGCAGGAGCACCTGCCCGTGC 96 CGTGCCAGTGGAACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 315 − 0 37 1 0 CGCGAATGCCCTCTTTGGGGGCCGAGGGCACCG 97 ATGTCACATAGCACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 350 + 0 37 1 0 CGCGAATGCCGACAGTGGCAGCGTCCCCTCCAC 98 GGGGCCCAGCCAACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 385 − 0 37 1 0 CGCGAATGCCCCGCTGACTTCCTCTTCAGCGAG 99 ATGGGAGTGCCCACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 420 + 0 37 1 0 CGCGAATGCCGCAGCATGTGCATCACCCATTC 100 ATGAAGAAGCGCACGCGTGGCGGATGT CHAF1A NM_005483 13 19 + 455 − 0 37 1 0 CGCGAATGCCACCTGCCCACCCCACCTCACCTG 101 GCCGTCGTGCCTACGCGTGGCGGATGT RBBP8 NM_002894 15 18 + 0 + 0 37 1 0 CGCGAATGCCATGAAGAAAGAAAAATGAATGA 102 TAGCTTGGAAGATACGCGTGGCGGATGT RBBP8 NM_002894 15 18 + 35 − 0 37 1 0 CGCGAATGCCGATTCATACTCTTCATGTGTTGTC 103 CGATCAAACATACGCGTGGCGGATGT RBBP8 NM_002894 15 18 + 70 + 0 37 1 0 CGCGAATGCCCTGTTTGGCAGACAGTTTCTCCCA 104 AGCAGCAGATGACGCGTGGCGGATGT RBBP8 NM_002894 15 18 + 105 − 0 37 1 0 CGCGAATGCCTAGTTTCTTTGTGGCAGTAGACA 105 ATTCCTCCTCTTACGCGTGGCGGATGT RBBP8 NM_002894 15 18 + 140 + 0 37 1 0 CGCGAATGCCCACAGTAAGATTTTTTTCTGTTTA 106 ATTATGGCTTCACGCGTGGCGGATGT RBBP8 NM_002894 17 18 + −22 + 0 37 1 0 CGCGAATGCCATTTAATTCATTTTTCCCCCAGAG 107 AGACTAGCTTGACGCGTGGCGGATGT RBBP8 NM_002894 17 18 + 13 − 0 37 1 0 CGCGAATGCCTTTTTCCGAACCACCTCAATATGA 108 GGAAAATTTTGACGCGTGGCGGATGT RBBP8 NM_002894 17 18 + 48 + 0 37 1 0 CGCGAATGCCAGAGGAGAGAAGAAAACTGCTT 109 GGGCACACGTGTAACGCGTGGCGGATGT RBBP8 NM_002894 17 18 + 83 − 0 37 1 0 CGCGAATGCCAGTATCTACATTAGTACTTACAA 110 TTTCACATTCCTACGCGTGGCGGATGT PDGFRA NM_006206 8 4 + −12 + 0 37 1 0 CGCGAATGCCTTTTTTTAAAAGGTATCGAAGCA 111 AATTAAAGCTGAACGCGTGGCGGATGT PDGFRA NM_006206 8 4 + 23 − 0 37 1 0 CGCGAATGCCAGTATAATGGCCACTGTCTTCTTC 112 CTTAGCACGGAACGCGTGGCGGATGT PDGFRA NM_006206 8 4 + 58 + 0 37 1 0 CGCGAATGCCATTGTAGCTCAAAATGAAGATGC 113 TGTGAAGAGCTAACGCGTGGCGGATGT PDGFRA NM_006206 8 4 + 93 − 0 37 1 0 CGCGAATGCCCCCTTTACATACCTTGAGTTAACA 114 GTTCAAAAGTAACGCGTGGCGGATGT RB1 NM_000321 17 13 + 0 + 0 37 1 0 CGCGAATGCCGAAGTACATCTCAGAATCTTGAT 115 TCTGGAACAGATACGCGTGGCGGATGT RB1 NM_000321 17 13 + 35 − 0 37 1 0 CGCGAATGCCAAATTAAGCACATTCAGAATCCA 116 TGGGAAAGACAAACGCGTGGCGGATGT RB1 NM_000321 17 13 + 70 + 0 37 1 0 CGCGAATGCCAAAAGCCTTTGATTTTTACAAAG 117 TGATCGAAAGTTACGCGTGGCGGATGT RB1 NM_000321 17 13 + 105 − 0 37 1 0 CGCGAATGCCCATTTCTCTTGTCAAGTTGCCTTC 118 TGCTTTGATAAACGCGTGGCGGATGT RB1 NM_000321 17 13 + 140 + 0 37 1 0 CGCGAATGCCATAAAACATTTAGAACGATGTGA 119 ACATCGAATCATACGCGTGGCGGATGT RB1 NM_000321 17 13 + 175 − 0 37 1 0 CGCGAATGCCTTTAGCTACTTACTGAGAGCCAT 120 GCAAGGGATTCCACGCGTGGCGGATGT EPHA3 NM_182644 7 3 + 0 + 0 37 1 0 CGCGAATGCCCAGGAACAAGAAACAAGTTATAC 121 CATTCTGAGGGCACGCGTGGCGGATGT EPHA3 NM_182644 7 3 + 35 − 0 37 1 0 CGCGAATGCCGCTTGAGGCTACTGATGGTAACA 122 TTTGTGCCTCTTACGCGTGGCGGATGT EPHA3 NM_182644 7 3 + 70 + 0 37 1 0 CGCGAATGCCCTGACACTATATACGTATTCCAA 123 ATCCGAGCCCGAACGCGTGGCGGATGT EPHA3 NM_182644 7 3 + 105 − 0 37 1 0 CGCGAATGCCAACTTGCGGCTGTTCGTCCCATAT 124 CCAGCGGCTGTACGCGTGGCGGATGT EPHA3 NM_182644 7 3 + 140 + 0 37 1 0 CGCGAATGCCTGAGTTTGAAACTAGTCCAGACT 125 GTATGTATTATTACGCGTGGCGGATGT EPHA3 NM_182644 7 3 + 175 − 0 37 1 0 CGCGAATGCCGCAAGATCCCTGCCCCCTCCTCT 126 AGACTGCATTGAACGCGTGGCGGATGT RET NM_020630 2 10 + 0 + 0 37 1 0 CGCGAATGCCTGGCATTGGGCCTCTACTTCTCGA 127 GGGATGCTTACACGCGTGGCGGATGT RET NM_020630 2 10 + 35 − 0 37 1 0 CGCGAATGCCGTGCCGGCTGCCTGGTCCACATA 128 CAGCTTCTCCCAACGCGTGGCGGATGT RET NM_020630 2 10 + 70 + 0 37 1 0 CGCGAATGCCGCCCTTGCTGTACGTCCATGCCCT 129 GCGGGACGCCCACGCGTGGCGGATGT RET NM_020630 2 10 + 105 − 0 37 1 0 CGCGAATGCCATGCTGGCCCAGGCGGAAGCTGG 130 GCACCTCCTCAGACGCGTGGCGGATGT RET NM_020630 2 10 + 140 + 0 37 1 0 CGCGAATGCCCTCTACGGCACGTACCGCACACG 131 GCTGCATGAGAAACGCGTGGCGGATGT RET NM_020630 2 10 + 175 − 0 37 1 0 CGCGAATGCCGGAGGCCGGTGTCCTCCTGGATG 132 CAGATCCAGTTGACGCGTGGCGGATGT RET NM_020630 2 10 + 210 + 0 37 1 0 CGCGAATGCCTCTACCTTAACCGGAGCCTGGAC 133 CATAGCTCCTGGACGCGTGGCGGATGT RET NM_020630 2 10 + 245 − 0 37 1 0 CGCGAATGCCGGGGCGGCTCCCTTACTGCGGAC 134 ACTGAGCTTCTCACGCGTGGCGGATGT PIK3CA NM_006218 10 3 + −8 + 0 0 2 0 CGCGAATGCCTTTTACAGAGTAACAGACTAGCT 135 AGAGACAATGAAACGCGTGGCGGATGT PIK3CA NM_006218 10 3 + 27 − 0 0 2 0 CGCGAATGCCATTGCTTTGAGCTGTTCTTTGTCA 136 TTTTCCCTTAAACGCGTGGCGGATGT PIK3CA NM_006218 10 3 + 62 + 0 23 1 1 CGCGAATGCCTTCTACACGAGATCCTCTCTCTGA 137 AATCACTGAGCACGCGTGGCGGATGT PIK3CA NM_006218 10 3 + 97 − 0 37 1 0 CGCGAATGCCCACTTACCTGTGACTCCATAGAA 138 AATCTTTCTCCTACGCGTGGCGGATGT PDGFRA NM_006206 11 4 + −22 + 0 37 1 0 CGCGAATGCCGCCTCTCTCTCTTGTCACGTAGCC 139 CTGCGTTCTGAACGCGTGGCGGATGT PDGFRA NM_006206 11 4 + 13 − 0 37 1 0 CGCGAATGCCCCAACAGCACCAGGACTGCAGCA 140 GCCACCGTGAGTACGCGTGGCGGATGT PDGFRA NM_006206 11 4 + 48 + 0 37 1 0 CGCGAATGCCTGATTGTGATCATCTCACTTATTG 141 TCCTGGTTGTCACGCGTGGCGGATGT PDGFRA NM_006206 11 4 + 83 − 0 37 1 0 CGCGAATGCCTAGTTTTATGAGAAAATATCTAC 142 CTGTTTCCAAATACGCGTGGCGGATGT NFKB1 NM_003998 4 4 + −50 + 0 37 1 0 CGCGAATGCCGAGAAGCCTCACAGTTTCTTTTG 143 GTTTCTGTTTGTACGCGTGGCGGATGT NFKB1 NM_003998 4 4 + −15 − 0 37 1 0 CGCGAATGCCTTGAAGGTATGGGCCATCTGCTA 144 AAAACAAAAACAACGCGTGGCGGATGT NFKB1 NM_003998 4 4 + 20 + 0 37 1 0 CGCGAATGCCATATTAGAGCAACCTAAACAGGT 145 AAGATTAAAGGGACGCGTGGCGGATGT NFKB1 NM_003998 4 4 + 55 − 0 37 1 0 CGCGAATGCCTATTAGACACTGGAATCTAACAT 146 TTAAAGTCCCACACGCGTGGCGGATGT EPHA4 NM_004438 9 2 − −13 + 0 37 1 0 CGCGAATGCCGTTTATCACTCAGGTGTAAGAAC 147 ATATGTGGACCCACGCGTGGCGGATGT EPHA4 NM_004438 9 2 − 22 − 0 37 1 0 CGCGAATGCCCTCGCACTGCTTGGTTGGGATCTT 148 CGTACGTAAAGACGCGTGGCGGATGT EPHA4 NM_004438 9 2 − 57 + 0 37 1 0 CGCGAATGCCAGTTTGCCAAAGAAATTGACGCA 149 TCCTGCATTAAGACGCGTGGCGGATGT EPHA4 NM_004438 9 2 − 92 − 0 37 1 0 CGCGAATGCCCTCAGACACTTACCAACTCCTAT 150 AACTTTTTCAATACGCGTGGCGGATGT PALB2 NM_024675 4 16 − −12 + 0 37 1 0 CGCGAATGCCTTTTAATTACAGAGGCAAAGAAA 151 ACCAATTTTTGAACGCGTGGCGGATGT PALB2 NM_024675 4 16 − 23 − 0 37 1 0 CGCGAATGCCCTCAGCAAAAGTTAGTATAGTCT 152 CCTCAGGGGGCAACGCGTGGCGGATGT PALB2 NM_024675 4 16 − 58 + 0 37 1 0 CGCGAATGCCGTCCAAGGGATGCAAGAAGCTCT 153 GCTTGGTACTACACGCGTGGCGGATGT PALB2 NM_024675 4 16 − 89 − 11 37 1 0 CGCGAATGCCAGCTTACCAAATAACAATGTTGT 154 TCATAATAGTAGACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 0 + 0 37 1 0 CGCGAATGCCTGGGAAGAGATCAGTGGTGTGGA 155 TGAACATTACACACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 35 − 0 37 1 0 CGCGAATGCCCCATGACATTGCACACCTGGTAA 156 GTCCTGATGGGTACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 70 + 0 37 1 0 CGCGAATGCCACCACAGTCAAAACAATTGGCTG 157 AGAACAAACTGGACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 105 − 0 37 1 0 CGCGAATGCCTCCACATAAATCTTCTGAGCTGA 158 GTTCCTGGGGACACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 140 + 0 37 1 0 CGCGAATGCCGCTCAAGTTCACTCTACGAGACT 159 GCAATAGCATTCACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 175 − 0 37 1 0 CGCGAATGCCGTTGAATGTCTCCTTGCAAGTTCC 160 TAAAACCAATGACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 210 + 0 37 1 0 CGCGAATGCCCTGTACTACATGGAGTCTGATGA 161 TGATCATGGGGTACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 245 − 0 37 1 0 CGCGAATGCCTGTCAATCTTTGTAAACTGATGCT 162 CTCGAAATTTCACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 280 + 0 37 1 0 CGCGAATGCCCCATTGCAGCTGATGAAAGTTTC 163 ACTCAAATGGATACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 315 − 0 37 1 0 CGCGAATGCCATCTCAGTGTTGAGCTTCAGAAT 164 ACGGTCCCCAAGACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 350 + 0 37 1 0 CGCGAATGCCTAGAGAAGTAGGTCCTGTCAACA 165 AGAAGGGATTTTACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 385 − 0 37 1 0 CGCGAATGCCGGCAACACAAGCACCAACATCTT 166 GAAATGCCAAATACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 420 + 0 37 1 0 CGCGAATGCCTTGGTGTCTGTGAGAGTATACTTC 167 AAAAAGTGCCCACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 455 − 0 37 1 0 CGCGAATGCCTGTCTGGAAACATAGCCAGATTC 168 TTCACTGTAAATACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 490 + 0 37 1 0 CGCGAATGCCCGGTACCCATGGACTCCCAGTCC 169 CTGGTGGAGGTTACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 525 − 0 37 1 0 CGCGAATGCCTCTTCCTCCTTAGAATTGTTGACA 170 CAAGACCCTCTACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 560 + 0 37 1 0 CGCGAATGCCTCCTCCAAGGATGTACTGCAGTA 171 CAGAAGGCGAATACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 595 − 0 37 1 0 CGCGAATGCCAGCATTGCAGGAACACTTGCCAA 172 TGGGTACAAGCCACGCGTGGCGGATGT EPHA3 NM_005233 3 3 + 630 + 0 37 1 0 CGCGAATGCCGGCTATGAAGAAAGAGGTTTTAT 173 GTGCCAAGGTAAACGCGTGGCGGATGT KSR2 NM_173598 13 12 − −32 + 6 37 1 0 CGCGAATGCCTATCCATCTTTCTCTCTCTTTATCT 174 TTCTCAGGTTACGCGTGGCGGATGT KSR2 NM_173598 13 12 − −1 − 0 37 1 0 CGCGAATGCCGGCTTCTTTGGTGCATTTGTTGTG 175 GCACTTTAACCACGCGTGGCGGATGT KSR2 NM_173598 13 12 − 34 + 0 37 1 0 CGCGAATGCCCCACCCTGTCATCTTCTGATCATC 176 CACCGAGGAGGACGCGTGGCGGATGT KSR2 NM_173598 13 12 − 69 − 0 37 1 0 CGCGAATGCCCCCCTGCCCCTAGGGCAGTAAGT 177 GTTAAATAGTTAACGCGTGGCGGATGT NTRK3 NM_001012338 2 15 − 0 + 0 37 1 0 CGCGAATGCCGTGGGAGGACACACCATGCTCCC 178 CATTCGCTGGATACGCGTGGCGGATGT NTRK3 NM_001012338 2 15 − 35 − 0 37 1 0 CGCGAATGCCTAGTGAACTTCCGGTACATGATG 179 CTTTCAGGAGGCACGCGTGGCGGATGT NTRK3 NM_001012338 2 15 − 70 + 0 37 1 0 CGCGAATGCCCAGAGAGTGATGTATGGAGCTTC 180 GGGGTGATCCTCACGCGTGGCGGATGT NTRK3 NM_001012338 2 15 − 105 − 0 37 1 0 CGCGAATGCCAACCATGGCTGCTTTCCATAGGT 181 GAAGATCTCCCAACGCGTGGCGGATGT NTRK3 NM_001012338 2 15 − 140 + 0 37 1 0 CGCGAATGCCCCAACTCTCAAACACGGAGGTAA 182 AAAGGGGGTGCGACGCGTGGCGGATGT RPS6KA1 NM_002953 4 1 + −29 + 0 37 1 0 CGCGAATGCCCTGCCCTGCTTCCTGCTCTGCCTT 183 CTCAGGTCTTCACGCGTGGCGGATGT RPS6KA1 NM_002953 4 1 + 6 − 0 37 1 0 CGCGAATGCCTGCCCACTGTCAGGCCGGGTGAC 184 TTTCCGCACCAGACGCGTGGCGGATGT RPS6KA1 NM_002953 4 1 + 41 + 0 37 1 0 CGCGAATGCCCCTGTATGCTATGAAGGTGCTGA 185 AGAAGGCAACGCACGCGTGGCGGATGT RPS6KA1 NM_002953 4 1 + 76 − 0 37 1 0 CGCGAATGCCGTTCTGCACAGGGAGGTGTCCCC 186 ACTCACCTTTCAACGCGTGGCGGATGT PKN1 NM_002741 7 19 + 0 + 0 37 1 0 CGCGAATGCCGGACCCTGGAGGTACGAGTGGTG 187 GGCTGCAGAGACACGCGTGGCGGATGT PKN1 NM_002741 7 19 + 35 − 0 37 1 0 CGCGAATGCCGAGGGGGTAGGGTTCCACGGGAT 188 GGTCTCTGGGAGACGCGTGGCGGATGT PKN1 NM_002741 7 19 + 70 + 0 37 1 0 CGCGAATGCCAATGGGGGGACCTGGGACCCCAG 189 ACAGCCGCCCCCACGCGTGGCGGATGT PKN1 NM_002741 7 19 + 105 − 0 37 1 0 CGCGAATGCCGCTGTAAAGGCCCCGGGCTGGGC 190 GGCTCAGGAAGGACGCGTGGCGGATGT PKN1 NM_002741 7 19 + 140 + 0 37 1 0 CGCGAATGCCCGAAGCGGAAGCCTCAGTGGCCG 191 GAGCAGCCTCAAACGCGTGGCGGATGT PKN1 NM_002741 7 19 + 175 − 0 37 1 0 CGCGAATGCCACGATGCCCACTCACTGGTGTTC 192 TCGGCTTCTGCTACGCGTGGCGGATGT KSR2 NM_173598 16 12 − 0 + 0 37 1 0 CGCGAATGCCAGCCAAGAAGAAGAGCAAACCC 193 TTGAACCTCAAGAACGCGTGGCGGATGT KSR2 NM_173598 16 12 − 35 − 0 37 1 0 CGCGAATGCCGGGGATGTTCTCGCAGCTGCCTA 194 CGCTGCTGTGGAACGCGTGGCGGATGT KSR2 NM_173598 16 12 − 70 + 0 37 1 0 CGCGAATGCCTCTCAGCAGCGCTCCCCGCTGCT 195 GTCCGAGCGCTCACGCGTGGCGGATGT KSR2 NM_173598 16 12 − 105 − 0 37 1 0 CGCGAATGCCGGAAAGGTGCGTGTCCCACAAAG 196 AAGGAGCGGAGGACGCGTGGCGGATGT KSR2 NM_173598 16 12 − 140 + 0 37 1 0 CGCGAATGCCTGCCTTCCACCCCTCCTGTTCACA 197 CTGAGGCCAACACGCGTGGCGGATGT KSR2 NM_173598 16 12 − 175 − 0 37 1 0 CGCGAATGCCAGTGCAGCCCGGCAGGGTGACTT 198 ACTTGCAGAGAAACGCGTGGCGGATGT CHAF1A NM_005483 4 19 + −42 + 0 37 1 0 CGCGAATGCCTGAAATAACCCGTGTTTAAAGAT 199 AAACGTCTTCTGACGCGTGGCGGATGT CHAF1A NM_005483 4 19 + −7 − 0 37 1 0 CGCGAATGCCTAGAGCCTTTGACGAATTTCTTA 200 GTTATCTGAAAAACGCGTGGCGGATGT CHAF1A NM_005483 4 19 + 28 + 0 37 1 0 CGCGAATGCCCAGAGAAGAACAAGCTCAGACT 201 GCAAAGAGTAAGAACGCGTGGCGGATGT CHAF1A NM_005483 4 19 + 63 − 0 37 1 0 CGCGAATGCCGCACAGCAGGTTAATTTTCTATTT 202 CAGGGAAAATGACGCGTGGCGGATGT RBBP8 NM_002894 2 18 + −16 + 0 37 1 0 CGCGAATGCCGAGCATATTAAGCAAGATGAACA 203 TCTCGGGAAGCAACGCGTGGCGGATGT RBBP8 NM_002894 2 18 + 19 − 0 37 1 0 CGCGAATGCCACTAGATGTATCTGCAGAGTTAG 204 GGCTTCCACAGCACGCGTGGCGGATGT RBBP8 NM_002894 2 18 + 54 + 0 37 1 0 CGCGAATGCCGACTTTAAGGACCTTTGGACAAA 205 ACTAAAAGAATGACGCGTGGCGGATGT RBBP8 NM_002894 2 18 + 89 − 0 37 1 0 CGCGAATGCCAAGAAAAGATTTTACCTTGTACT 206 TCTCTATCATGAACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 0 + 0 37 1 0 CGCGAATGCCATGAGCCGGGGCGCGGGCGCGCT 207 TCAGCGCCGGACACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 35 − 0 37 1 0 CGCGAATGCCGCTTAACCAGGGTCAGCGAGATG 208 AGGTAGGTCGTTACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 66 + 27 37 1 0 CGCGAATGCCAAGCTCGAGTCGGTGCCTCCGCC 209 GCCGCCTTCTCCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 109 − 23 37 1 0 CGCGAATGCCCTCGGAGCCTCTGGCACCGGCGG 210 CGCCGGCCGCGGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 140 + 0 37 1 0 CGCGAATGCCCGAGACTGGGGATCCTGGCAGCC 211 CCCGAGGCGCGGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 175 − 0 37 1 0 CGCGAATGCCGAAGAGACGTTCGTGCCGCTTCT 212 TGCCCGGCTCCTACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 210 + 0 37 1 0 CGCGAATGCCCACCGGCAGGATGCGCTGTGGAT 213 CAGCACGAGCAGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 245 − 0 37 1 0 CGCGAATGCCACAGGGCTGGGGGCTCCGCGCCC 214 CCGGTGCCCGCGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 280 + 0 37 1 0 CGCGAATGCCCCCCGGCTCCGGCCAGTCCGGCC 215 CGCCCAGTCTCCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 315 − 0 37 1 0 CGCGAATGCCACGGCCCAGAGGGAGAGGCGGC 216 GGCCGGGAGCGGGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 350 + 0 37 1 0 CGCGAATGCCCCCTCCGGGACCCCCGCTCTCCG 217 GGGGACTGAGCCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 385 − 0 37 1 0 CGCGAATGCCGGAGGAGGTGGGGGCGCCCCCA 218 GGCTTGGGGTCGGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 420 + 0 37 1 0 CGCGAATGCCCGGCGCCCCCTGCTCAGCAGCCC 219 GAGCTGGGGCGGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 455 − 0 37 1 0 CGCGAATGCCGGATGCCGCCGCCCGCCCGGCCT 220 TCGGGCTCCGGGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 490 + 0 37 1 0 CGCGAATGCCCTGGCTCATCCTCTCCGCACCCTG 221 GCACCGGCAGCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 525 − 0 37 1 0 CGCGAATGCCGGAGCCGGCGGAGGAGGCGCCA 222 CCTTGAGCCTCCGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 560 + 0 37 1 0 CGCGAATGCCCAAGCCTTGCAAGACCGTGACCA 223 CGAGTGGAGCCAACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 595 − 0 37 1 0 CGCGAATGCCCAGGCGGCTACCCGCGCCCTTGC 224 CCCCGCCGGCTTACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 630 + 0 37 1 0 CGCGAATGCCTCATGGCCCGAAAGCGAGGGCAA 225 GCCCAGGGTCAAACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 665 − 0 37 1 0 CGCGAATGCCCCGAAGCTCCAGTCCCGGCGCTG 226 CTCTTTGACCCCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 700 + 25 37 1 0 CGCGAATGCCTCTCTGCCGCCGCCACCGCCGCC 227 GCCGCCGGGGGAACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 735 − 0 37 1 0 CGCGAATGCCCCGACCCCACCAGAGGTCGAAGC 228 TGTAGAGCCCCCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 770 + 0 37 1 0 CGCGAATGCCGGCTGGGGCTGGAGCCCGAGGG 229 AAGTTGTCCCCTCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 805 − 0 37 1 0 CGCGAATGCCGTCACTGTTGTCCAAGGTCTTACT 230 CTTGCCTTTCCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 840 + 0 37 1 0 CGCGAATGCCTTGCATCCGGGACCGCCTGCCGG 231 CTCTCCTCCTCCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 875 − 0 37 1 0 CGCGAATGCCCAGTGGCTGGACTCGGAGTTGGT 232 GGGAGGGTTAGCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 910 + 0 37 1 0 CGCGAATGCCCTGTCACCGCTGCTTCCGCGCAG 233 CCCCCCGGGCCTACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 945 − 0 37 1 0 CGCGAATGCCCCCGGAGCTGGAGGCTCCAGAGT 234 GATTGGAGGTGCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 980 + 0 37 1 0 CGCGAATGCCGCTGAAACGGGGCCGGGAGGGG 235 GGCCGAGCATCCAACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 1015 − 0 37 1 0 CGCGAATGCCGCCGCTGATAAACTTGAGCATCT 236 TGCGGTCACGAGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 1050 + 0 37 1 0 CGCGAATGCCATCTTCACCAAGAGCACAGGAGG 237 GCCTCCTGGCTCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 1085 − 0 37 1 0 CGCGAATGCCCAGAAGACAGGCTGGGGGGTCC 238 GGGAAGGGGCCCGACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 1120 + 0 37 1 0 CGCGAATGCCGCAGCGGGTCCAGGGAGCTGCTG 239 GGCGCCGAGCTCACGCGTGGCGGATGT CENTG1 NM_001122772 19 12 − 1155 − 0 37 1 0 CGCGAATGCCCTCAAGCCCTGACTCAACTCACT 240 AGGGGAAGCGCGACGCGTGGCGGATGT PKN1 NM_002741 1 19 + −56 + 2 37 1 0 CGCGAATGCCGCTCCTCTGGCCGCCCCTCCCTCC 241 GCGCGGGGACCACGCGTGGCGGATGT PKN1 NM_002741 1 19 + −25 − 0 37 1 0 CGCGAATGCCCGCTGGCCATGTCCTCCTGCCGC 242 CCGCCAGGGGTCACGCGTGGCGGATGT PKN1 NM_002741 1 19 + 9 + 0 37 1 0 CGCGAATGCCGACGCCGTGCAGGTAGGCGCACC 243 TGCGTCTGGAGTACGCGTGGCGGATGT PKN1 NM_002741 1 19 + 41 − 32 37 1 0 CGCGAATGCCGGGGTCCGCCGGCGCCGTCCGGT 244 CGCCCCGGGACTACGCGTGGCGGATGT PIK3CA NM_006218 5 3 + 0 + 0 37 1 0 CGCGAATGCCTATATAAGAAGCTGTATAATGCT 245 TGGGAGGATGCCACGCGTGGCGGATGT PIK3CA NM_006218 5 3 + 35 − 0 37 1 0 CGCGAATGCCAATAAAGGCTTTCTTTAGCCATC 246 AACATCAAATTGACGCGTGGCGGATGT PIK3CA NM_006218 5 3 + 70 + 0 37 1 0 CGCGAATGCCCTCAACTGCCAATGGACTGTTTT 247 ACAATGCCATCTACGCGTGGCGGATGT PIK3CA NM_006218 5 3 + 105 − 0 37 1 0 CGCGAATGCCATATATGGTGTAGCTGTGGAAAT 248 GCGTCTGGAATAACGCGTGGCGGATGT PIK3CA NM_006218 5 3 + 140 + 0 37 1 0 CGCGAATGCCGAATGGAGAAACATCTACAAAAT 249 CCCTTTGGGTTAACGCGTGGCGGATGT PIK3CA NM_006218 5 3 + 175 − 0 37 1 0 CGCGAATGCCTGCACAAAGAATTTTTATTCTGA 250 GTGCACTATTTAACGCGTGGCGGATGT PIK3CA NM_006218 5 3 + 210 + 0 37 1 0 CGCGAATGCCACCTACGTGAATGTAAATATTCG 251 AGACATTGATAAACGCGTGGCGGATGT PIK3CA NM_006218 5 3 + 245 − 0 37 1 0 CGCGAATGCCCTATAAAATAATAAGCATCAGCA 252 TTTGACTTTACCACGCGTGGCGGATGT KSR2 NM_173598 12 12 − −8 + 0 37 1 0 CGCGAATGCCCCCTACGGATCCAGCAAGGTTAG 253 TCCGGACAGAGTACGCGTGGCGGATGT KSR2 NM_173598 12 12 − 27 − 0 37 1 0 CGCGAATGCCCTTCCGTAGAGGGTTGTTGATGT 254 CACACGGAACGGACGCGTGGCGGATGT KSR2 NM_173598 12 12 − 62 + 0 37 1 0 CGCGAATGCCCCACCTCGCTATTCAGACCTGCA 255 CATCAGTCAGACACGCGTGGCGGATGT KSR2 NM_173598 12 12 − 97 − 0 37 1 0 CGCGAATGCCGCCTTACCTTGTTGATTTTGTTGG 256 TTTTGGGGAGCACGCGTGGCGGATGT PDGFRA NM_006206 2 4 + −46 + 0 37 1 0 CGCGAATGCCTAATGCTGTTTCTGTTGACTTTTG 257 ACTTTTCTAGTACGCGTGGCGGATGT PDGFRA NM_006206 2 4 + −11 − 0 37 1 0 CGCGAATGCCGAACGCCGGATGGGAAGTCCCCA 258 TAGCTCTGGGAAACGCGTGGCGGATGT PDGFRA NM_006206 2 4 + 24 + 0 37 1 0 CGCGAATGCCCTGGTCTTAGGCTGTCTTCTCACA 259 GGTACGGAGCCACGCGTGGCGGATGT PDGFRA NM_006206 2 4 + 59 − 0 37 1 0 CGCGAATGCCACAAGACACCCAAACAAGGAAC 260 TCAGAGAGGACTGACGCGTGGCGGATGT EPHA4 NM_004438 14 2 − 0 + 0 37 1 0 CGCGAATGCCGTCCACCATCTGCTCCCCTGAACT 261 TGATTTCAAATACGCGTGGCGGATGT EPHA4 NM_004438 14 2 − 35 − 0 37 1 0 CGCGAATGCCCTACTCCATTCCAAGTTCACAGA 262 TGTCTCGTTGACACGCGTGGCGGATGT EPHA4 NM_004438 14 2 − 70 + 0 37 1 0 CGCGAATGCCCCCTCAGAATACAGGTGGCCGCC 263 AGGACATTTCCTACGCGTGGCGGATGT EPHA4 NM_004438 14 2 − 105 − 0 37 1 0 CGCGAATGCCGTCACCAGCTCCACATTTCTTGCA 264 TACCACATTATACGCGTGGCGGATGT EPHA4 NM_004438 14 2 − 140 + 0 37 1 0 CGCGAATGCCCCCAGCAAGTGCCGACCCTGTGG 265 AAGTGGGGTCCAACGCGTGGCGGATGT EPHA4 NM_004438 14 2 − 175 − 0 37 1 0 CGCGAATGCCTGGTGGTCTTCAAGCCATTCTGCT 266 GTGGGGTGTAGACGCGTGGCGGATGT EPHA4 NM_004438 14 2 − 210 + 0 37 1 0 CGCGAATGCCAAGTCTCCATCACTGACCTCCTA 267 GCTCATACCAATACGCGTGGCGGATGT EPHA4 NM_004438 14 2 − 245 − 0 37 1 0 CGCGAATGCCGACACTCCATTCACAGCCCAGAT 268 TTCAAAGGTGTAACGCGTGGCGGATGT EPHA4 NM_004438 14 2 − 280 + 0 37 1 0 CGCGAATGCCCAAATATAACCCTAACCCAGACC 269 AATCAGTTTCTGACGCGTGGCGGATGT EPHA4 NM_004438 14 2 − 315 − 0 37 1 0 CGCGAATGCCTCAATTCCTACCTGCTTGGTTGGT 270 GGTCACAGTGAACGCGTGGCGGATGT EPHA3 NM_005233 11 3 + 0 + 0 37 1 0 CGCGAATGCCGTGAATTTGGAGAGGTGTGCAGT 271 GGTCGCTTAAAAACGCGTGGCGGATGT EPHA3 NM_005233 11 3 + 35 − 0 37 1 0 CGCGAATGCCTTAATGGCCACTGAAATCTCTTTT 272 TTTGAAGGAAGACGCGTGGCGGATGT EPHA3 NM_005233 11 3 + 70 + 0 37 1 0 CGCGAATGCCGACCCTGAAAGTTGGCTACACAG 273 AAAAGCAGAGGAACGCGTGGCGGATGT EPHA3 NM_005233 11 3 + 105 − 0 37 1 0 CGCGAATGCCCTGTCCCATAATGCTTGCTTCTCC 274 CAGGAAGTCTCACGCGTGGCGGATGT EPHA3 NM_005233 11 3 + 140 + 0 37 1 0 CGCGAATGCCTTTGACCACCCCAATATCATTCG 275 ACTGGAAGGAGTACGCGTGGCGGATGT EPHA3 NM_005233 11 3 + 175 − 0 37 1 0 CGCGAATGCCAGGTCTTATGACTACTTTACTTAC 276 TTTTGGTAACAACGCGTGGCGGATGT RB1 NM_000321 23 13 + 0 + 0 37 1 0 CGCGAATGCCACATTCAAACGTGTTTTGATCAA 277 AGAAGAGGAGTAACGCGTGGCGGATGT RB1 NM_000321 23 13 + 35 − 0 37 1 0 CGCGAATGCCTGAAGACCGAGTTATAGAATACT 278 ATAATAGAATCAACGCGTGGCGGATGT RB1 NM_000321 23 13 + 70 + 0 37 1 0 CGCGAATGCCTGCAGAGACTGAAAACAAATATT 279 TTGCAGTATGCTACGCGTGGCGGATGT RB1 NM_000321 23 13 + 105 − 0 37 1 0 CGCGAATGCCCCAATCAAAGGATACTTTTGACC 280 TACCCTGGTGGAACGCGTGGCGGATGT RB1 NM_000321 23 13 + 140 + 0 37 1 0 CGCGAATGCCAAAAATCTAATGTAATGGGTCCA 281 CCAAAACATTAAACGCGTGGCGGATGT RB1 NM_000321 23 13 + 175 − 0 37 1 0 CGCGAATGCCGGGCTAGAGCAAAAACAAAAAA 282 GTAGATTATTTATACGCGTGGCGGATGT RB1 NM_000321 23 13 + 210 + 0 37 1 0 CGCGAATGCCCCTACCTTGTCACCAATACCTCAC 283 ATTCCTCGAAGACGCGTGGCGGATGT RB1 NM_000321 23 13 + 245 − 0 37 1 0 CGCGAATGCCGAATCCGTAAGGGTGAACTAGGA 284 AACTTGTAAGGGACGCGTGGCGGATGT RB1 NM_000321 23 13 + 280 + 0 37 1 0 CGCGAATGCCCTGGAGGGAACATCTATATTTCA 285 CCCCTGAAGAGTACGCGTGGCGGATGT RB1 NM_000321 23 13 + 315 − 0 37 1 0 CGCGAATGCCGTTGGTGTTGGCAGACCTTCTGA 286 AATTTTATATGGACGCGTGGCGGATGT RB1 NM_000321 23 13 + 350 + 0 37 1 0 CGCGAATGCCAAAAATGACTCCAAGATCAAGGT 287 GTGTGTTTTCTCACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 0 + 0 37 1 0 CGCGAATGCCTGGGAAGAAGTCAGTGGCTACGA 288 TGAAAACCTGAAACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 35 − 0 37 1 0 CGCGAATGCCCGAAGACATTGCACACCTGGTAG 289 GTGCGGATGGTGACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 70 + 0 37 1 0 CGCGAATGCCAGCCCAACCAGAACAATTGGCTG 290 CTCACCACCTTCACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 105 − 0 37 1 0 CGCGAATGCCTCTGTGTAGATGCGATGGGCCCC 291 CCGCCGGTTGATACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 140 + 0 37 1 0 CGCGAATGCCGATGCGCTTCACTGTGAGAGACT 292 GCAGCAGCCTCCACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 175 − 0 37 1 0 CGCGAATGCCGTTGAAGGTCTCCTTGCAGGATC 293 CTGGGACATTAGACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 210 + 0 37 1 0 CGCGAATGCCTTGTATTACTATGAGACTGACTCT 294 GTCATTGCCACACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 245 − 0 37 1 0 CGCGAATGCCGGTAGGGGGCCTCAGACCAGAA 295 GGCTGACTTCTTGACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 280 + 0 37 1 0 CGCGAATGCCTCAAAGTAGACACCATTGCTGCA 296 GATGAGAGCTTCACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 315 − 0 37 1 0 CGCGAATGCCACCTTCATCAGCCTTCCCCCAAA 297 GTCCACCTGGGAACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 350 + 0 37 1 0 CGCGAATGCCAAACACAGAAGTCAGGAGCTTTG 298 GGCCTCTTACTCACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 385 − 0 37 1 0 CGCGAATGCCTCCATAATCCTGAAAAGCGAGGT 299 AAAAACCATTCCACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 420 + 0 37 1 0 CGCGAATGCCGCCTGTATGTCTCTTCTTTCTGTC 300 CGTGTCTTCTTACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 455 − 0 37 1 0 CGCGAATGCCCTGCAAAATTTTGCACAATGCTG 301 GGACACTTTTTGACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 490 + 0 37 1 0 CGCGAATGCCTGTTTCCAGAGACTATGACAGGG 302 GCAGAGAGCACAACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 525 − 0 37 1 0 CGCGAATGCCTTGGGGATGCATGTGCCCCGAGC 303 AATCACCAGAGAACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 560 + 0 37 1 0 CGCGAATGCCCGCAGAGGAAGTGGACGTGCCCA 304 TCAAACTCTACTACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 595 − 0 37 1 0 CGCGAATGCCCCCAATAGGCACCATCCATTCCC 305 CATCCCCGTTGCACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 630 + 0 37 1 0 CGCGAATGCCCGATGCACCTGCAAGCCTGGCTA 306 TGAGCCTGAGAAACGCGTGGCGGATGT EPHB1 NM_004441 3 3 + 665 − 0 37 1 0 CGCGAATGCCAGAGGCTCCAAAGCTTACCCTTG 307 CATGCCACGCTGACGCGTGGCGGATGT NFKB1 NM_003998 22 4 + 0 + 0 37 1 0 CGCGAATGCCGAGACATGAAACAGCTGGCTGAA 308 GATGTGAAGCTGACGCGTGGCGGATGT NFKB1 NM_003998 22 4 + 35 − 0 37 1 0 CGCGAATGCCTCTGGATCAGGAATTTCTAGTAA 309 CTTATACAGCTGACGCGTGGCGGATGT NFKB1 NM_003998 22 4 + 70 + 0 37 1 0 CGCGAATGCCCAAAAACTGGGCTACTCTGGCGC 310 AGAAATTAGGTCACGCGTGGCGGATGT NFKB1 NM_003998 22 4 + 105 − 0 37 1 0 CGCGAATGCCAGGACTCAGCCGGAAGGCATTAT 311 TAAGTATCCCCAACGCGTGGCGGATGT NFKB1 NM_003998 22 4 + 140 + 0 37 1 0 CGCGAATGCCGCTCCTTCCAAAACACTTATGGA 312 CAACTATGAGGTACGCGTGGCGGATGT RBBP8 NM_002894 13 18 + −26 + 0 37 1 0 CGCGAATGCCTTGCATGCTCTTTCCCTTTACCTA 313 AGATGTATCCTACGCGTGGCGGATGT RBBP8 NM_002894 13 18 + 9 − 0 37 1 0 CGCGAATGCCTGCTCCCGGATCTATACTCCACTG 314 GATATTTTCAAACGCGTGGCGGATGT RBBP8 NM_002894 13 18 + 44 + 0 37 1 0 CGCGAATGCCGACCTTTCTCAGTATAAAATGGA 315 TGTTACTGTAATACGCGTGGCGGATGT RBBP8 NM_002894 13 18 + 79 − 0 37 1 0 CGCGAATGCCGTTTTGGTTTTACTTTTTAACTTA 316 CCTTTGTATCTACGCGTGGCGGATGT NFKB1 NM_003998 7 4 + 0 + 0 37 1 0 CGCGAATGCCCTTCGCAAACCTGGGTATACTTC 317 ATGTGACAAAGAACGCGTGGCGGATGT NFKB1 NM_003998 7 4 + 35 − 0 37 1 0 CGCGAATGCCTGTCATTCGTGCTTCCAGTGTTTC 318 AAATACTTTTTACGCGTGGCGGATGT NFKB1 NM_003998 7 4 + 70 + 0 37 1 0 CGCGAATGCCGAGGCGTGTATAAGGGGCTATAA 319 TCCTGGACTCTTACGCGTGGCGGATGT NFKB1 NM_003998 7 4 + 105 − 0 37 1 0 CGCGAATGCCCTTCTGCTTGCAAATAGGCAAGG 320 TCAGGGTGCACCACGCGTGGCGGATGT NFKB1 NM_003998 7 4 + 140 + 0 37 1 0 CGCGAATGCCGTGGAGGGGACCGGCAGCTGGG 321 AGGTAAGCATCATACGCGTGGCGGATGT NFKB1 NM_003998 10 4 + −24 + 0 37 1 0 CGCGAATGCCACACTTCAATGTGATTGTTTGCA 322 GATGACATCCAGACGCGTGGCGGATGT NFKB1 NM_003998 10 4 + 11 − 0 37 1 0 CGCGAATGCCACTCCACCATTTTCTTCCTCTTCA 323 TAAAATCGAATACGCGTGGCGGATGT NFKB1 NM_003998 10 4 + 46 + 0 37 1 0 CGCGAATGCCCTGGGAAGGATTTGGAGATTTTT 324 CCCCCACAGATGACGCGTGGCGGATGT NFKB1 NM_003998 10 4 + 81 − 0 37 1 0 CGCGAATGCCTAATAATAATAATAAATCACTTA 325 CTTGTCTATGAAACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 0 + 0 37 1 0 CGCGAATGCCATGCCTCCACGACCATCATCAGG 326 TGAACTGTGGGGACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 35 − 0 37 1 0 CGCGAATGCCATTCTACTAGGATTCTTGGGGGC 327 ATCAAGTGGATGACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 70 + 0 37 1 0 CGCGAATGCCGTTTACTACCAAATGGAATGATA 328 GTGACTTTAGAAACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 105 − 0 37 1 0 CGCGAATGCCTGCTTTATGGTTATTAATGTAGCC 329 TCACGGAGGCAACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 140 + 0 37 1 0 CGCGAATGCCTGAACTATTTAAAGAAGCAAGAA 330 AATACCCCCTCCACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 175 − 0 37 1 0 CGCGAATGCCGAAAATGTAAGAAGATTCATCTT 331 GAAGAAGTTGATACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 210 + 0 37 1 0 CGCGAATGCCGTAAGTGTTACTCAAGAAGCAGA 332 AAGGGAAGAATTACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 245 − 0 37 1 0 CGCGAATGCCGCCGAAGGTCACAAAGTCGTCTT 333 GTTTCATCAAAAACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 280 + 0 37 1 0 CGCGAATGCCTTTTTCAACCCTTTTTAAAAGTAA 334 TTGAACCAGTAACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 315 − 0 37 1 0 CGCGAATGCCATTTCTCGATTGAGGATCTTTTCT 335 TCACGGTTGCCACGCGTGGCGGATGT PIK3CA NM_006218 2 3 + 350 + 0 37 1 0 CGCGAATGCCTGGTATGATACAATATCCTATTCT 336 AAAATGCAAATACGCGTGGCGGATGT EPHA4 NM_004438 10 2 − −44 + 13 37 1 0 CGCGAATGCCGTGTTTGAGGAAAATAATTCTCT 337 TTTTTAAAAAAAACGCGTGGCGGATGT EPHA4 NM_004438 10 2 − −1 − 0 37 1 0 CGCGAATGCCCGCTTCTTGTTTGGCTTTACTGTA 338 TTTACTCCGTCACGCGTGGCGGATGT EPHA4 NM_004438 10 2 − 30 + 0 37 1 0 CGCGAATGCCAGCGGATGAAGAGAAACATTTGA 339 ATCAAGGTACAAACGCGTGGCGGATGT EPHA4 NM_004438 10 2 − 65 − 0 37 1 0 CGCGAATGCCCATGCTCTAAAAATAGAATTTTTT 340 AATCCAATTTTACGCGTGGCGGATGT NFKB1 NM_003998 23 4 + 0 + 0 37 1 0 CGCGAATGCCGTCTCTGGGGGTACAGTCAGAGA 341 GCTGGTGGAGGCACGCGTGGCGGATGT NFKB1 NM_003998 23 4 + 35 − 0 37 1 0 CGCGAATGCCCTTCAATTGCTTCGGTGTAGCCCA 342 TTTGTCTCAGGACGCGTGGCGGATGT NFKB1 NM_003998 23 4 + 70 + 0 37 1 0 CGCGAATGCCTGATCCAGGCAGCCTCCAGCCCA 343 GTGAAGACCACCACGCGTGGCGGATGT NFKB1 NM_003998 23 4 + 105 − 0 37 1 0 CGCGAATGCCGAGGCAGGCGAGAGAGGCAGCG 344 AGTGGGCCTGAGAACGCGTGGCGGATGT NFKB1 NM_003998 23 4 + 140 + 0 37 1 0 CGCGAATGCCCACAAGGCAGCAAATAGGTAAA 345 AAAAAAGACAAAAACGCGTGGCGGATGT NFKB1 NM_003998 20 4 + −8 + 0 37 1 0 CGCGAATGCCCTTCGCAGGAGCAGATCCCCTGG 346 TGGAGAACTTTGACGCGTGGCGGATGT NFKB1 NM_003998 20 4 + 27 − 0 37 1 0 CGCGAATGCCATTTTCCCAAGAGTCATCCAGGT 347 CATAGAGAGGCTACGCGTGGCGGATGT NFKB1 NM_003998 20 4 + 62 + 0 37 1 0 CGCGAATGCCGCAGGAGAGGATGAAGGAGTTG 348 TGCCTGGAACCACACGCGTGGCGGATGT NFKB1 NM_003998 20 4 + 97 − 0 37 1 0 CGCGAATGCCCACTCACCTGCCAGCTGGTGGCC 349 ATATCTAGAGGCACGCGTGGCGGATGT PIK3CA NM_006218 4 3 + 0 + 0 37 1 0 CGCGAATGCCGGCAAATAATAGTGGTGATCTGG 350 GTAATAGTTTCTACGCGTGGCGGATGT PIK3CA NM_006218 4 3 + 35 − 0 37 1 0 CGCGAATGCCATTTTCAGAGTATACTTCTGCTTG 351 TCATTATTTGGACGCGTGGCGGATGT PIK3CA NM_006218 4 3 + 70 + 0 37 1 0 CGCGAATGCCCAACCATGACTGTGTACCAGAAC 352 AAGTAATTGCTGACGCGTGGCGGATGT PIK3CA NM_006218 4 3 + 105 − 0 37 1 0 CGCGAATGCCTAGCAACATACTTCGAGTTTTTTT 353 CCTGATTGCTTACGCGTGGCGGATGT PIK3CA NM_006218 4 3 + 140 + 0 37 1 0 CGCGAATGCCTCCTCTGAACAACTAAAACTCTG 354 TGTTTTAGAATAACGCGTGGCGGATGT PIK3CA NM_006218 4 3 + 175 − 0 37 1 0 CGCGAATGCCCACATCCACACACTTTTAAAATA 355 TACTTGCCCTGAACGCGTGGCGGATGT PIK3CA NM_006218 4 3 + 210 + 0 37 1 0 CGCGAATGCCATGAATACTTCCTAGAAAAATAT 356 CCTCTGAGTCAGACGCGTGGCGGATGT PIK3CA NM_006218 4 3 + 241 − 9 37 1 0 CGCGAATGCCTAATATTTTGAAACTTGTTACTCA 357 CCTTATACTGAACGCGTGGCGGATGT GUCY2F NM_001522 4 X − −22 + 0 37 1 0 CGCGAATGCCCATGTCTCTCCTTCTTTTGCAGCT 358 TATCGCATTCAACGCGTGGCGGATGT GUCY2F NM_001522 4 X − 13 − 0 37 1 0 CGCGAATGCCGATTTTGAAGAATTGTAACAGTG 359 CTGAGACTGACAACGCGTGGCGGATGT GUCY2F NM_001522 4 X − 48 + 0 37 1 0 CGCGAATGCCTGAGTGAGGGCTATGAAGTGGAG 360 CTTCGAGGAAGAACGCGTGGCGGATGT GUCY2F NM_001522 4 X − 83 − 0 37 1 0 CGCGAATGCCCAAAAAACAAAAAGACATTATAC 361 CTTGAGCTCTGTACGCGTGGCGGATGT PKN1 NM_002741 21 19 + 0 + 0 37 1 0 CGCGAATGCCTCCCCATTCCCAGGGGATGATGA 362 GGAGGAGGTCTTACGCGTGGCGGATGT PKN1 NM_002741 21 19 + 35 − 0 37 1 0 CGCGAATGCCGGGGGTAGCGAACCTCGTCGTTG 363 ACGATGCTGTCGACGCGTGGCGGATGT PKN1 NM_002741 21 19 + 70 + 0 37 1 0 CGCGAATGCCGCTTCCTGTCGGCCGAAGCCATC 364 GGCATCATGAGAACGCGTGGCGGATGT PKN1 NM_002741 21 19 + 105 − 0 37 1 0 CGCGAATGCCCCCCAGGCCCGCCCCACGTCGGG 365 GGTCCTCACCCTACGCGTGGCGGATGT PKN1 NM_002741 21 19 + 140 + 0 37 1 0 CGCGAATGCCAGGGGCAGTGGGGCCCAGGAGG 366 GGACAGATCCTGAACGCGTGGCGGATGT PKN1 NM_002741 21 19 + 175 − 0 37 1 0 CGCGAATGCCTTCCTCCGAAGCAGCTTGGAGGA 367 AGGAGCACTGTGACGCGTGGCGGATGT PKN1 NM_002741 21 19 + 210 + 0 37 1 0 CGCGAATGCCCCCAGAGCGGAGGCTGGGATCTA 368 GCGAGAGAGATGACGCGTGGCGGATGT PKN1 NM_002741 21 19 + 245 − 0 37 1 0 CGCGAATGCCCACCCTGAAGAAGGGCTGTTTCT 369 TCACATCTTCTGACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 0 + 0 37 1 0 CGCGAATGCCATATGATGCAGCCATTGACCTGT 370 TTACACGTTCATACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 35 − 0 37 1 0 CGCGAATGCCTCCCAAAATGAAGGTAGCTACAC 371 AGTATCCAGCACACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 70 + 0 37 1 0 CGCGAATGCCATTGGAGATCGTCACAATAGTAA 372 CATCATGGTGAAACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 105 − 0 37 1 0 CGCGAATGCCTTTAAACAGAGAAAACCATTACT 373 TGTCCATCGTCTACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 140 + 0 37 1 0 CGCGAATGCCATGTTTTGGTGTTCTTAATTTATT 374 CAAGACATTTTACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 175 − 0 37 1 0 CGCGAATGCCAAGAAATTATGTTATAGTTTGAT 375 ATATGCAGATACACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 210 + 0 37 1 0 CGCGAATGCCATTTTTGAAAGCTGTTTCATATAG 376 ATTTTGGACACACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 245 − 0 37 1 0 CGCGAATGCCTTATAACCAAATTTTTTCTTCTTG 377 TGATCCAAAAAACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 280 + 0 37 1 0 CGCGAATGCCACGAGAACGTGTGCCATTTGTTT 378 TGACACAGGATTACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 315 − 0 37 1 0 CGCGAATGCCGCATTCTTGGGCTCCTTTACTAAT 379 CACTATTAAGAACGCGTGGCGGATGT PIK3CA NM_006218 20 3 + 350 + 0 37 1 0 CGCGAATGCCACAAAGACAAGAGAATTTGAGA 380 GGTGAGCTCGAGCACGCGTGGCGGATGT RBBP8 NM_002894 4 18 + −22 + 0 37 1 0 CGCGAATGCCTTATTTATTTTTTGACCTTTAGAG 381 ATGCACAAAGAACGCGTGGCGGATGT RBBP8 NM_002894 4 18 + 13 − 0 37 1 0 CGCGAATGCCCTCAGCTGTTGATTTTTGGTGAAG 382 AATTCTTCTAGACGCGTGGCGGATGT RBBP8 NM_002894 4 18 + 48 + 0 37 1 0 CGCGAATGCCGGAACAGCAGAAAGTCCTTCATG 383 AAACCATTAAAGACGCGTGGCGGATGT RBBP8 NM_002894 4 18 + 83 − 0 37 1 0 CGCGAATGCCAAGACCTAAGTGCCAGACTCACC 384 GATCTTCTAAAAACGCGTGGCGGATGT NTRK3 NM_001012338 8 15 − −18 + 0 37 1 0 CGCGAATGCCGTTTCTGGTTCCTCACAGGTATCC 385 ATAGCAGTTGGACGCGTGGCGGATGT NTRK3 NM_001012338 8 15 − 17 − 0 37 1 0 CGCGAATGCCGAACCACCAACAGGACACAGGC 386 AAAAGCAGCAAGTACGCGTGGCGGATGT NTRK3 NM_001012338 8 15 − 52 + 0 37 1 0 CGCGAATGCCTCTTCGTCATGATCAACAAATAT 387 GGTCGACGGTCCACGCGTGGCGGATGT NTRK3 NM_001012338 8 15 − 87 − 0 37 1 0 CGCGAATGCCAATGAAACTCCCACCTTACCCTT 388 CATTCCAAATTTACGCGTGGCGGATGT EPHA4 NM_004438 6 2 − 0 + 0 37 1 0 CGCGAATGCCAAAAATGATGGCAGATTTACAGT 389 CATTCAGCTGGTACGCGTGGCGGATGT EPHA4 NM_004438 6 2 − 35 − 0 37 1 0 CGCGAATGCCACTTCATCCCAGACCCAATGCCA 390 CGAAGCATGCCCACGCGTGGCGGATGT EPHA4 NM_004438 6 2 − 70 + 0 37 1 0 CGCGAATGCCATTTATCTGATATGAGCTATGTGC 391 ATCGTGATCTGACGCGTGGCGGATGT EPHA4 NM_004438 6 2 − 105 − 0 37 1 0 CGCGAATGCCACCAAGTTGCTGTTCACCAGGAT 392 GTTCCGTGCGGCACGCGTGGCGGATGT EPHA4 NM_004438 6 2 − 140 + 0 37 1 0 CGCGAATGCCCTGCAAAGTGTCTGATTTTGGCA 393 TGTCCCGAGTGCACGCGTGGCGGATGT EPHA4 NM_004438 6 2 − 175 − 0 37 1 0 CGCGAATGCCCCTGGTGGTGTAAGCTGCTTCCG 394 GATCATCCTCAAACGCGTGGCGGATGT EPHA4 NM_004438 6 2 − 210 + 0 37 1 0 CGCGAATGCCGTAAGAAAGATCGGTGACATCTG 395 GGCTTTCACTCTACGCGTGGCGGATGT NTRK3 NM_001012338 18 15 − 0 + 0 37 1 0 CGCGAATGCCATGGATGTCTCTCTTTGCCCAGCC 396 AAGTGTAGTTTACGCGTGGCGGATGT NTRK3 NM_001012338 18 15 − 35 − 0 37 1 0 CGCGAATGCCCCAGCCAGACGCTTCCCAGCAAG 397 AAAATCCGCCAGACGCGTGGCGGATGT NTRK3 NM_001012338 18 15 − 70 + 0 37 1 0 CGCGAATGCCACTATGTGGGCTCCGTGCTGGCT 398 TGCCCTGCAAATACGCGTGGCGGATGT NTRK3 NM_001012338 18 15 − 105 − 0 37 1 0 CGCGAATGCCCGCCGGCAATTGATCTCAGTCTT 399 GCTGCAGACACAACGCGTGGCGGATGT NTRK3 NM_001012338 18 15 − 140 + 0 37 1 0 CGCGAATGCCGCCGGACGATGGGAACCTCTTCC 400 CCCTCCTGGAAGACGCGTGGCGGATGT NTRK3 NM_001012338 18 15 − 175 − 0 37 1 0 CGCGAATGCCACTGGCGTTCCCATTGCTGTTCCC 401 TGAATCCTGCCACGCGTGGCGGATGT NTRK3 NM_001012338 18 15 − 210 + 0 37 1 0 CGCGAATGCCATCAACATCACGGACATCTCAAG 402 GAATATCACTTCACGCGTGGCGGATGT NTRK3 NM_001012338 18 15 − 245 − 0 37 1 0 CGCGAATGCCGAGGCAGGCTGGGGAGCGGCCG 403 CCTGACTTACATGACGCGTGGCGGATGT PIK3CA NM_006218 7 3 + −17 + 0 37 1 0 CGCGAATGCCTTTAAAATTTTACATAGGTGGAA 404 TGAATGGCTGAAACGCGTGGCGGATGT PIK3CA NM_006218 7 3 + 18 − 0 37 1 0 CGCGAATGCCCAGCACGAGGAAGATCAGGAAT 405 GTATATATCATAAACGCGTGGCGGATGT PIK3CA NM_006218 7 3 + 53 + 0 37 1 0 CGCGAATGCCCTCGACTTTGCCTTTCCATTTGCT 406 CTGTTAAAGGCACGCGTGGCGGATGT PIK3CA NM_006218 7 3 + 88 − 0 37 1 0 CGCGAATGCCCTTCTGAAATACTTTACCTCTTTA 407 GCACCCTTTCGACGCGTGGCGGATGT NTRK3 NM_001012338 15 15 − −36 + 0 37 1 0 CGCGAATGCCTGTTTTAAAATTTCCCTTTATTTG 408 TCAATCTTGCAACGCGTGGCGGATGT NTRK3 NM_001012338 15 15 − −1 − 0 37 1 0 CGCGAATGCCCGAGAGTGTGGTGAGCCGGTTAC 409 TTGACAGGTTTCACGCGTGGCGGATGT NTRK3 NM_001012338 15 15 − 34 + 0 37 1 0 CGCGAATGCCTGGCAGCTCTTCCAGACGCTGAG 410 TCTTCGGGAATTACGCGTGGCGGATGT NTRK3 NM_001012338 15 15 − 69 − 0 37 1 0 CGCGAATGCCTTCAGTACCTGGACAGTCTTCAA 411 AACCAAACTTACACGCGTGGCGGATGT PALB2 NM_024675 6 16 − −27 + 0 37 1 0 CGCGAATGCCAATTTTTGGCTGCTTTGTTTTATT 412 TAGGTTCCAGTACGCGTGGCGGATGT PALB2 NM_024675 6 16 − 8 − 0 37 1 0 CGCGAATGCCGATTATACACATCAGGCACTGGA 413 ACTATCTGTAATACGCGTGGCGGATGT PALB2 NM_024675 6 16 − 43 + 0 37 1 0 CGCGAATGCCTCGTGTGTGTAGCTTTGGGAAAT 414 TTGGAAATCAGAACGCGTGGCGGATGT PALB2 NM_024675 6 16 − 78 − 0 37 1 0 CGCGAATGCCACAAATCACTCCTTGGGAATTAC 415 ATACCTGATCTCACGCGTGGCGGATGT EPHB1 NM_004441 16 3 + −16 + 0 37 1 0 CGCGAATGCCGGCTCTTTCCTCCTAGAGACCTCC 416 TGAGAATAGGCACGCGTGGCGGATGT EPHB1 NM_004441 16 3 + 19 − 0 37 1 0 CGCGAATGCCTTCAGGATCTTCTTCTGATGGCCT 417 GCCAAGGTGATACGCGTGGCGGATGT EPHB1 NM_004441 16 3 + 54 + 0 37 1 0 CGCGAATGCCCAGCATTCATTCTATGAGGGTCC 418 AGATAAGTCAGTACGCGTGGCGGATGT EPHB1 NM_004441 16 3 + 89 − 0 37 1 0 CGCGAATGCCAAGAAACAAGAGTTCTCATGCCA 419 TTGCCGTTGGTGACGCGTGGCGGATGT NFKB1 NM_003998 11 4 + 0 + 0 37 1 0 CGCGAATGCCTTTGCCATTGTCTTCAAAACTCCA 420 AAGTATAAAGAACGCGTGGCGGATGT NFKB1 NM_003998 11 4 + 35 − 0 37 1 0 CGCGAATGCCGGACAAACACAGAGGCTGGTTTT 421 GTAATATTAATAACGCGTGGCGGATGT NFKB1 NM_003998 11 4 + 70 + 0 37 1 0 CGCGAATGCCAGCTTCGGAGGAAATCTGACTTG 422 GAAACTAGTGAAACGCGTGGCGGATGT NFKB1 NM_003998 11 4 + 105 − 0 37 1 0 CGCGAATGCCCCTTTGATTTCAGGATAGTAGAG 423 GAAAGGTTTTGGACGCGTGGCGGATGT PDGFRA NM_006206 23 4 + 0 + 0 37 1 0 CGCGAATGCCCTCGCAGACCTCTGAAGAGAGTG 424 CCATTGAGACGGACGCGTGGCGGATGT PDGFRA NM_006206 23 4 + 35 − 0 37 1 0 CGCGAATGCCGTCCTCTCTCTTGATGAAGGTGG 425 AACTGCTGGAACACGCGTGGCGGATGT PDGFRA NM_006206 23 4 + 70 + 0 37 1 0 CGCGAATGCCGAGACCATTGAAGACATCGACAT 426 GATGGATGACATACGCGTGGCGGATGT PDGFRA NM_006206 23 4 + 105 − 0 37 1 0 CGCGAATGCCAGCTGTCTTCCACCAGGTCTGAA 427 GAGTCTATGCCGACGCGTGGCGGATGT PDGFRA NM_006206 23 4 + 140 + 0 37 1 0 CGCGAATGCCTCCTGTAACTGGCGGATTCGAGG 428 GGTTCCTTCCACACGCGTGGCGGATGT RB1 NM_000321 20 13 + 0 + 0 37 1 0 CGCGAATGCCTGTATCGGCTAGCCTATCTCCGG 429 CTAAATACACTTACGCGTGGCGGATGT RB1 NM_000321 20 13 + 35 − 0 37 1 0 CGCGAATGCCTCTAATTCTGGGTGCTCAGACAG 430 AAGGCGTTCACAACGCGTGGCGGATGT RB1 NM_000321 20 13 + 70 + 0 37 1 0 CGCGAATGCCACATATCATCTGGACCCTTTTCCA 431 GCACACCCTGCACGCGTGGCGGATGT RB1 NM_000321 20 13 + 105 − 0 37 1 0 CGCGAATGCCCAAATGCCTGTCTCTCATGAGTTC 432 ATACTCATTCTACGCGTGGCGGATGT RB1 NM_000321 20 13 + 140 + 0 37 1 0 CGCGAATGCCGACCAAGTAAGAAAATCAAGCA 433 CTTCACCTTCTCTACGCGTGGCGGATGT NFKB1 NM_003998 14 4 + 0 + 0 37 1 0 CGCGAATGCCGAACCATGGACACTGAATCTAAA 434 AAGGACCCTGAAACGCGTGGCGGATGT NFKB1 NM_003998 14 4 + 35 − 0 37 1 0 CGCGAATGCCTTTACAGTGTTTTTGTCATCACTT 435 TTGTCACAACCACGCGTGGCGGATGT NFKB1 NM_003998 14 4 + 70 + 0 37 1 0 CGCGAATGCCCCTCTTTGGGAAAGTTATTGAAA 436 CCACAGAGCAAGACGCGTGGCGGATGT NFKB1 NM_003998 14 4 + 105 − 0 37 1 0 CGCGAATGCCACCATTCCCAACGGTGGCCTCGC 437 TGGGCTCCTGATACGCGTGGCGGATGT NFKB1 NM_003998 14 4 + 140 + 0 37 1 0 CGCGAATGCCGAGGTCACTCTAACGTATGCAAC 438 AGGAACAAAAGAACGCGTGGCGGATGT NFKB1 NM_003998 14 4 + 175 − 0 37 1 0 CGCGAATGCCTGTGTGCTCACTTACCCTGAACTC 439 CAGCACTCTCTACGCGTGGCGGATGT PALB2 NM_024675 12 16 − 0 + 0 37 1 0 CGCGAATGCCTTAAAGGAGAAATTAGCATTCTT 440 GAAAAGGGAATAACGCGTGGCGGATGT PALB2 NM_024675 12 16 − 35 − 0 37 1 0 CGCGAATGCCATTCACTTACCTGAAGGCGGGCT 441 AGTGTCTTGCTGACGCGTGGCGGATGT PALB2 NM_024675 12 16 − 70 + 0 37 1 0 CGCGAATGCCCGTATTCTCAAATTAAGGTGTTAT 442 AGTACAAACAAACGCGTGGCGGATGT PALB2 NM_024675 12 16 − 105 − 0 37 1 0 CGCGAATGCCTTTAAAGTTTTATAGAGTCAAGA 443 ACTGTTTTTAAAACGCGTGGCGGATGT PALB2 NM_024675 12 16 − 140 + 0 37 1 0 CGCGAATGCCGAAAACGTATTTCTGGGGCTGTT 444 TTTGTCTCCTCTACGCGTGGCGGATGT PALB2 NM_024675 12 16 − 175 − 0 37 1 0 CGCGAATGCCAGAATGCTTAATCTTTTCAGCTCT 445 TTGGGCACGCTACGCGTGGCGGATGT PALB2 NM_024675 12 16 − 210 + 0 37 1 0 CGCGAATGCCATTAAGAAAACAGTAGAAGAAC 446 AAGATTGTTTGTCACGCGTGGCGGATGT PALB2 NM_024675 12 16 − 245 − 0 37 1 0 CGCGAATGCCCTGAGTGTTTTAGCTGCGGTGAG 447 AGATCCTGCTGAACGCGTGGCGGATGT PALB2 NM_024675 12 16 − 280 + 0 37 1 0 CGCGAATGCCGTAAATCTAGACCATTCACTTAT 448 GCCTGCTTTATTACGCGTGGCGGATGT RPS6KA1 NM_002953 20 1 + −11 + 0 37 1 0 CGCGAATGCCCTTTTCTGCAGATATACTCCATTT 449 GCCAACGGTCCACGCGTGGCGGATGT RPS6KA1 NM_002953 20 1 + 24 − 0 37 1 0 CGCGAATGCCCGATCCGGGTTAGGATTTCCTCT 450 GGTGTGTCACTGACGCGTGGCGGATGT RPS6KA1 NM_002953 20 1 + 59 + 0 37 1 0 CGCGAATGCCGCAGTGGGAAGTTTACCCTCAGT 451 GGGGGAAATTGGACGCGTGGCGGATGT RPS6KA1 NM_002953 20 1 + 94 − 0 37 1 0 CGCGAATGCCTACAGACTCACCTTGGCTGTCTCT 452 GAAACTGTGTTACGCGTGGCGGATGT CENTG1 NM_014770 17 12 − −40 + 0 37 1 0 CGCGAATGCCGCCAGGAGCCTCCCCTCTTACTG 453 CCCTTCTCCCGTACGCGTGGCGGATGT CENTG1 NM_014770 17 12 − −5 − 0 37 1 0 CGCGAATGCCAAGTCCATTCCTGGCTATTGATC 454 ACAGCCTCTGTAACGCGTGGCGGATGT CENTG1 NM_014770 17 12 − 30 + 0 37 1 0 CGCGAATGCCTGAGCCGCTCCATTCCTGAACTG 455 CGCCTGGTAGGTACGCGTGGCGGATGT CENTG1 NM_014770 17 12 − 65 − 0 37 1 0 CGCGAATGCCAGGTCAGCCTTCCCTATAGGCAA 456 GGGGACTGGGTTACGCGTGGCGGATGT EPHA3 NM_005233 13 3 + 0 + 0 37 1 0 CGCGAATGCCAAACACGATGCCCAGTTTACTGT 457 CATTCAGCTAGTACGCGTGGCGGATGT EPHA3 NM_005233 13 3 + 35 − 0 37 1 0 CGCGAATGCCACTTCATGCCAGATGCTATCCCTC 458 GAAGCATCCCCACGCGTGGCGGATGT EPHA3 NM_005233 13 3 + 70 + 0 37 1 0 CGCGAATGCCACCTGTCAGACATGGGCTATGTT 459 CACCGAGACCTCACGCGTGGCGGATGT EPHA3 NM_005233 13 3 + 105 − 0 37 1 0 CGCGAATGCCACCAAGTTACTGTTGATCAAGAT 460 GTTCCGAGCAGCACGCGTGGCGGATGT EPHA3 NM_005233 13 3 + 140 + 0 37 1 0 CGCGAATGCCGTGTAAGGTTTCTGATTTCGGACT 461 TTCGCGTGTCCACGCGTGGCGGATGT EPHA3 NM_005233 13 3 + 175 − 0 37 1 0 CGCGAATGCCTCTTGTTGTATAAGCAGCTTCTGG 462 GTCATCCTCCAACGCGTGGCGGATGT EPHA3 NM_005233 13 3 + 210 + 0 37 1 0 CGCGAATGCCGTGAGTAACTTAGATTTTCTCCTT 463 TTTTATCATTGACGCGTGGCGGATGT EPHA3 NM_005233 6 3 + −8 + 0 37 1 0 CGCGAATGCCCTTTACAGCTCCATCACCTGTCCT 464 GACGATTAAGAACGCGTGGCGGATGT EPHA3 NM_005233 6 3 + 27 − 0 37 1 0 CGCGAATGCCGGACAAAGAGATGCTATTTCTGG 465 AGGTCCGATCTTACGCGTGGCGGATGT EPHA3 NM_005233 6 3 + 62 + 0 37 1 0 CGCGAATGCCTGGCAAGAACCTGAACATCCTAA 466 TGGGATCATATTACGCGTGGCGGATGT EPHA3 NM_005233 6 3 + 97 − 0 37 1 0 CGCGAATGCCTCCCCACCTTTTCATAGTATTTGA 467 CCTCGTAGTCCACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 0 + 0 37 1 0 CGCGAATGCCAAAGGCAAAGGCATCACAATGCT 468 GGAAGAAATCAAACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 35 − 0 37 1 0 CGCGAATGCCCCGTCAAAGTGTACACCAATTTG 469 ATGGATGGGACTACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 70 + 0 37 1 0 CGCGAATGCCTCCCCGAGGCCACGGTGAAAGAC 470 AGTGGAGATTACACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 105 − 0 37 1 0 CGCGAATGCCTTGACCTCCCTGGTAGCCTGGCG 471 GGCAGCACATTCACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 140 + 0 37 1 0 CGCGAATGCCAGAAATGAAGAAAGTCACTATTT 472 CTGTCCATGGTAACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 175 − 0 37 1 0 CGCGAATGCCCAGCATGGACAACTGACATTTTA 473 GAAAGCGGAATGACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 210 + 0 37 1 0 CGCGAATGCCCTCGGGATCCATATGTGGTAATC 474 ATTATTTAATGGACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 245 − 0 37 1 0 CGCGAATGCCTTCAATGAAACCTTTCTCTGTACA 475 GGGAAGAGTTTACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 280 + 0 37 1 0 CGCGAATGCCATCAAACCCACCTTCAGCCAGTT 476 GGAAGCTGTCAAACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 315 − 0 37 1 0 CGCGAATGCCGCACCTCTACAACAAAATGTTTG 477 ACTTCATGCAGGACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 350 + 0 37 1 0 CGCGAATGCCGGGCCTACCCACCTCCCAGGATA 478 TCCTGGCTGAAAACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 385 − 0 37 1 0 CGCGAATGCCATCTCAGTGAGATTTTCAATCAG 479 AGTCAGATTGTTACGCGTGGCGGATGT PDGFRA NM_006206 7 4 + 420 + 0 37 1 0 CGCGAATGCCCACCACTGATGTGGAAAAGATTC 480 AGGAAATAAGGTACGCGTGGCGGATGT RET NM_020630 14 10 + 0 + 0 37 1 0 CGCGAATGCCGCCCGCTCCTCCTCATCGTGGAG 481 TACGCCAAATACACGCGTGGCGGATGT RET NM_020630 14 10 + 35 − 0 37 1 0 CGCGAATGCCTTGCGGCTCTCGCGGAGGAAGCC 482 CCGCAGGGAGCCACGCGTGGCGGATGT RET NM_020630 14 10 + 70 + 0 37 1 0 CGCGAATGCCAGTGGGGCCTGGCTACCTGGGCA 483 GTGGAGGCAGCCACGCGTGGCGGATGT RET NM_020630 14 10 + 105 − 0 37 1 0 CGCGAATGCCCCGCTCATCCGGGTGGTCCAGGG 484 AGCTGGAGTTGCACGCGTGGCGGATGT RET NM_020630 14 10 + 140 + 0 37 1 0 CGCGAATGCCGCCCTCACCATGGGCGACCTCAT 485 CTCATTTGCCTGACGCGTGGCGGATGT RET NM_020630 14 10 + 175 − 0 37 1 0 CGCGAATGCCTCTCGGCCAGATACTGCATCCCC 486 TGTGAGATCTGCACGCGTGGCGGATGT RET NM_020630 14 10 + 210 + 0 37 1 0 CGCGAATGCCTGAAGGTGCGTGCATATGGCTCT 487 GCACCCAGCCAGACGCGTGGCGGATGT NFKB1 NM_003998 18 4 + 0 + 0 37 1 0 CGCGAATGCCGTCTGAATGCCATTCATCTAGCC 488 ATGATGAGCAATACGCGTGGCGGATGT NFKB1 NM_003998 18 4 + 35 − 0 37 1 0 CGCGAATGCCCCAGCGGCCACCAGCAGCAGCAA 489 ACATGGCAGGCTACGCGTGGCGGATGT NFKB1 NM_003998 18 4 + 70 + 0 37 1 0 CGCGAATGCCGGCTGACGTCAATGCTCAGGAGC 490 AGAAGTCCGGGCACGCGTGGCGGATGT NFKB1 NM_003998 18 4 + 105 − 0 37 1 0 CGCGAATGCCGTTGTCGTGCTCCACAGCCAGGT 491 GCAGTGCTGTGCACGCGTGGCGGATGT NFKB1 NM_003998 18 4 + 140 + 0 37 1 0 CGCGAATGCCATCTCATTGGCAGGCTGCCTGCT 492 CCTGGAGGTGAAACGCGTGGCGGATGT EPHA3 NM_005233 15 3 + 0 + 0 37 1 0 CGCGAATGCCGTAATTAAAGCTGTAGATGAGGG 493 CTATCGACTGCCACGCGTGGCGGATGT EPHA3 NM_005233 15 3 + 35 − 0 37 1 0 CGCGAATGCCGCTGATACAAGGCAGCTGGGCAG 494 TCCATGGGGGGTACGCGTGGCGGATGT EPHA3 NM_005233 15 3 + 70 + 0 37 1 0 CGCGAATGCCTGATGCTGGACTGCTGGCAGAAA 495 GACAGGAACAACACGCGTGGCGGATGT EPHA3 NM_005233 15 3 + 105 − 0 37 1 0 CGCGAATGCCTCCAGAATACTAACAATCTGCTC 496 AAACTTGGGTCTACGCGTGGCGGATGT EPHA3 NM_005233 15 3 + 140 + 0 37 1 0 CGCGAATGCCCAAGCTTATCCGGAATCCCGGCA 497 GCCTGAAGATCAACGCGTGGCGGATGT EPHA3 NM_005233 15 3 + 175 − 0 37 1 0 CGCGAATGCCAAATTGAATGTGTCACCTTGCGG 498 CTGCACTGGTGAACGCGTGGCGGATGT PKN1 NM_002741 4 19 + −2 + 0 37 1 0 CGCGAATGCCAGGACCGGAAGCTGCTGCTGACA 499 GCCCAGCAGATGACGCGTGGCGGATGT PKN1 NM_002741 4 19 + 33 − 0 37 1 0 CGCGAATGCCCGGATGATGTCAATCTTGGTCTT 500 ACTGTCCTGCAAACGCGTGGCGGATGT PKN1 NM_002741 4 19 + 68 + 0 37 1 0 CGCGAATGCCCATGCAACTCCGCCGGGCGCTGC 501 AGGCCGGCCAGCACGCGTGGCGGATGT PKN1 NM_002741 4 19 + 103 − 0 37 1 0 CGCGAATGCCACCTTGGGTGTCATCCGGGGCTG 502 CCTGGTTCTCCAACGCGTGGCGGATGT EPHA4 NM_004438 17 2 − −36 + 0 37 1 0 CGCGAATGCCTTGGAAATTCATCTATTTTTCTTT 503 TGTTTTTTGCAACGCGTGGCGGATGT EPHA4 NM_004438 17 2 − −1 − 0 37 1 0 CGCGAATGCCTCTCCCTGAACAGATCTGGAATC 504 CAATAAGGTAACACGCGTGGCGGATGT EPHA4 NM_004438 17 2 − 34 + 0 37 1 0 CGCGAATGCCACTTGGGTGGATAGCAAGCCCTC 505 TGGAAGGAGGGGACGCGTGGCGGATGT EPHA4 NM_004438 17 2 − 69 − 0 37 1 0 CGCGAATGCCTTTACCCTTTGGATCAGAGAGCA 506 ACTCAGGACTTAACGCGTGGCGGATGT PIK3CA NM_006218 21 3 + 0 + 0 37 1 0 CGCGAATGCCGTTTCAGGAGATGTGTTACAAGG 507 CTTATCTAGCTAACGCGTGGCGGATGT PIK3CA NM_006218 21 3 + 35 − 0 37 1 0 CGCGAATGCCGAAAAGATTTATGAAGAGATTGG 508 CATGCTGTCGAAACGCGTGGCGGATGT PIK3CA NM_006218 21 3 + 70 + 0 37 1 0 CGCGAATGCCTCAATGATGCTTGGCTCTGGAAT 509 GCCAGAACTACAACGCGTGGCGGATGT PIK3CA NM_006218 21 3 + 105 − 0 37 1 0 CGCGAATGCCGGGTCTTTCGAATGTATGCAATG 510 TCATCAAAAGATACGCGTGGCGGATGT PIK3CA NM_006218 21 3 + 140 + 0 37 1 0 CGCGAATGCCTAGCCTTAGATAAAACTGAGCAA 511 GAGGCTTTGGAGACGCGTGGCGGATGT PIK3CA NM_006218 21 3 + 175 − 0 37 1 0 CGCGAATGCCCCATGATGTGCATCATTCATTTGT 512 TTCATGAAATAACGCGTGGCGGATGT PIK3CA NM_006218 21 3 + 210 + 0 37 1 0 CGCGAATGCCTGGCTGGACAACAAAAATGGATT 513 GGATCTTCCACAACGCGTGGCGGATGT PIK3CA NM_006218 21 3 + 245 − 0 37 1 0 CGCGAATGCCGTTATCTTTTCAGTTCAATGCATG 514 CTGTTTAATTGACGCGTGGCGGATGT GUCY2F NM_001522 3 X − 0 + 0 37 1 0 CGCGAATGCCGGCAAAGGCACAGAGGAAACCT 515 TCTGGCTGATTGGACGCGTGGCGGATGT GUCY2F NM_001522 3 X − 35 − 0 37 1 0 CGCGAATGCCGGGGCACAGGAAGGGGCTTCATG 516 AAGCCTTTTTTCACGCGTGGCGGATGT GUCY2F NM_001522 3 X − 70 + 0 37 1 0 CGCGAATGCCCACCAGTGGACAAAGATGGGTAA 517 GTGGAGTTCACAACGCGTGGCGGATGT GUCY2F NM_001522 3 X − 105 − 0 37 1 0 CGCGAATGCCGTGGCAGAGTATGGCATAGGAAG 518 ACTCCAATTAAAACGCGTGGCGGATGT GUCY2F NM_001522 3 X − 140 + 0 37 1 0 CGCGAATGCCACTTTCTTCACTACCTATTTGATG 519 TCTCCCCTGCCACGCGTGGCGGATGT GUCY2F NM_001522 3 X − 175 − 0 37 1 0 CGCGAATGCCCCACTGGTTGCAGGCCATGGCCC 520 ACTTGCCTGCAGACGCGTGGCGGATGT GUCY2F NM_001522 3 X − 210 + 0 37 1 0 CGCGAATGCCAGATTGCAGCCTTCCAAAGAAGA 521 AAAGCAGAAAGGACGCGTGGCGGATGT GUCY2F NM_001522 3 X − 245 − 0 37 1 0 CGCGAATGCCTGGCCATTACCTTATGGCTTGTTT 522 CTCACCAACTGACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 0 + 0 37 1 0 CGCGAATGCCATTTTCAGATTCTACTTCAAAGAC 523 TCCTCCTCAAGACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 35 − 0 37 1 0 CGCGAATGCCAAATACAGGAGATGACACTCGAG 524 TAGGTAATTCTTACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 70 + 0 37 1 0 CGCGAATGCCGGAGCTACCTCTAGTATCAAAAG 525 TGGTTTAGATTTACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 105 − 0 37 1 0 CGCGAATGCCCAGGCTGTAAAAGAGAAGGGGA 526 CAAACTTGTATTCACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 140 + 0 37 1 0 CGCGAATGCCGGAAAAAAAAACATCTGAAAAC 527 ACTCCCTTTTAGCACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 175 − 0 37 1 0 CGCGAATGCCGATCTAGTTTTTTCTAATCTAGAT 528 ATACAAGTGTTACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 210 + 0 37 1 0 CGCGAATGCCAAAATCTGAAGATAGTGCCCTTT 529 TCACACATCACAACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 245 − 0 37 1 0 CGCGAATGCCCTGGATAATGATCTTGTTCACTTC 530 AGACCCAAGACACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 280 + 0 37 1 0 CGCGAATGCCTCATCTAATAAACAGATACTTAT 531 AAATAAAAATATACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 315 − 0 37 1 0 CGCGAATGCCACTCAGTCCTATTCTGTTCACCTA 532 GGGATTCACTTACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 350 + 0 37 1 0 CGCGAATGCCACGGTAAAGATTCTAACACTGAT 533 AAACATTTGGAGACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 385 − 0 37 1 0 CGCGAATGCCCTTTTGGATGTTCGGCCTCCCAAT 534 GATTTCAGGGGACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 420 + 0 37 1 0 CGCGAATGCCGAAGAAAACTGAGGAAGAAAGT 535 GAACATGAAGTAAACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 455 − 0 37 1 0 CGCGAATGCCAGCATTTTCTTTATCAAAAGAAG 536 CTTGGGGGCAGCACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 490 + 0 37 1 0 CGCGAATGCCTTCCCTTTTCCAATGGATAATCAG 537 TTTTCCATGAAACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 525 − 0 37 1 0 CGCGAATGCCACAGATCCAGAGGTTTATCCATC 538 ACACAGTCTCCAACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 560 + 0 37 1 0 CGCGAATGCCCTGATCGATTTTCAGCTATTCAGC 539 GTCAAGAGAAAACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 595 − 0 37 1 0 CGCGAATGCCCTAAATTTGTTTTTAGAAGTCTCA 540 CTTCCTTGGCTACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 630 + 0 37 1 0 CGCGAATGCCGCAAGTGACTCTTTATGAGGCTT 541 TGAAGACCATTCACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 665 − 0 37 1 0 CGCGAATGCCATCTGAGGCCTTACGGCTTGAGG 542 AAAAGCCCTTTGACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 700 + 0 37 1 0 CGCGAATGCCGGCAACTGCACGTTGCCCAAAGA 543 TTCCCCAGGGGAACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 735 − 0 37 1 0 CGCGAATGCCAGGGCTGAAGGATGATGCATTCC 544 TGTGAACAGGGCACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 770 + 0 37 1 0 CGCGAATGCCTGAATAAATGCTCTCCAGACAAT 545 AAACCATCATTAACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 805 − 0 37 1 0 CGCGAATGCCGGAATTTTAAAGACAGCATTTTC 546 TTCTTTTATTTGACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 840 + 0 37 1 0 CGCGAATGCCTCTACGTCCACGTGAAAGTTTGG 547 AGACTGAGAATGACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 875 − 0 37 1 0 CGCGAATGCCTGAACATTTAACACAAACCTTTA 548 TGTCATCTAAAAACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 910 + 0 37 1 0 CGCGAATGCCAGGATTTTGATTAAAATGATTGC 549 TTGTGATTTCATACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 945 − 0 37 1 0 CGCGAATGCCCAGCACTCTAAAACAGAAATAAT 550 TGTTAGCTCTAAACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 980 + 0 37 1 0 CGCGAATGCCGTTCTCATGAGCCAATAAAAATA 551 CAAACCAGGTCAACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 1015 − 0 37 1 0 CGCGAATGCCTGAAGAACTGATGCAAGTTCACA 552 TCCTCCATGGTCACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 1050 + 0 37 1 0 CGCGAATGCCGTTAAATCCATGTAGAACTGGTA 553 AAATAAAGTCTCACGCGTGGCGGATGT RBBP8 NM_002894 12 18 + 1085 − 0 37 1 0 CGCGAATGCCCCTGTTTATGGTGTACACACCTTG 554 GTTGTTTTGTAACGCGTGGCGGATGT EPHB1 NM_004441 5 3 + 0 + 0 37 1 0 CGCGAATGCCGCGTCCCATCAGGTCCCCGCAAT 555 GTTATCTCCATCACGCGTGGCGGATGT EPHB1 NM_004441 5 3 + 35 − 0 37 1 0 CGCGAATGCCGGGTGCCACTCCAGAATGATGGA 556 CGTCTCATTGACACGCGTGGCGGATGT EPHB1 NM_004441 5 3 + 70 + 0 37 1 0 CGCGAATGCCTCCAAGGGAGACAGGTGGGCGG 557 GATGATGTGACCTACGCGTGGCGGATGT EPHB1 NM_004441 5 3 + 105 − 0 37 1 0 CGCGAATGCCGCGGTCTGCCCGGCACTTTTTGC 558 AGATGATGTTGTACGCGTGGCGGATGT EPHB1 NM_004441 5 3 + 140 + 0 37 1 0 CGCGAATGCCCGGAGCTGCTCCCGCTGTGACGA 559 CAATGTGGAGTTACGCGTGGCGGATGT EPHB1 NM_004441 5 3 + 175 − 0 37 1 0 CGCGAATGCCCGCGGCACTCCGTCAGGCCCAGC 560 TGCCTGGGCACAACGCGTGGCGGATGT EPHB1 NM_004441 5 3 + 210 + 0 37 1 0 CGCGAATGCCTCTCCATCAGCAGCCTGTGGGCC 561 CACACCCCCTACACGCGTGGCGGATGT EPHB1 NM_004441 5 3 + 245 − 0 37 1 0 CGCGAATGCCCTGGAGACTCCATTGATGGCCTG 562 GATGTCAAAGGTACGCGTGGCGGATGT EPHB1 NM_004441 5 3 + 280 + 0 37 1 0 CGCGAATGCCCAAGAGTCCCTTCCCCCCACAGC 563 ACGTCTCTGTCAACGCGTGGCGGATGT EPHB1 NM_004441 5 3 + 315 − 0 37 1 0 CGCGAATGCCGCCTCCAGACTTACCGGCTTGGT 564 TTGTGGTGATGTACGCGTGGCGGATGT RPS6KA1 NM_002953 9 1 + 0 + 0 37 1 0 CGCGAATGCCACTTTGGCCTGAGCAAAGAGGCC 565 ATTGACCACGAGACGCGTGGCGGATGT RPS6KA1 NM_002953 9 1 + 35 − 0 37 1 0 CGCGAATGCCTACTCCACTGTCCCGCAGAAAGA 566 ATAGGCCTTCTTACGCGTGGCGGATGT RPS6KA1 NM_002953 9 1 + 70 + 0 37 1 0 CGCGAATGCCCATGGCCCCTGAGGTCGTCAACC 567 GCCAGGGCCACTACGCGTGGCGGATGT RPS6KA1 NM_002953 9 1 + 105 − 0 37 1 0 CGCGAATGCCCAACACCCCATAGGACCACCAGT 568 CCGCACTATGGGACGCGTGGCGGATGT RPS6KA1 NM_002953 9 1 + 140 + 0 37 1 0 CGCGAATGCCATGGTGAGTGCCCAGACAGGGGT 569 AAAGGATCCAGCACGCGTGGCGGATGT RPS6KA1 NM_001006665 1 1 + −2 + 0 37 1 0 CGCGAATGCCGGATGGAGCAGGATCCCAAGCCG 570 CCCCGTCTGCGGACGCGTGGCGGATGT RPS6KA1 NM_001006665 1 1 + 33 − 0 37 1 0 CGCGAATGCCTGCTTCCTGGGAAGCCAGGGGAT 571 CAGGGCCCAGAGACGCGTGGCGGATGT RPS6KA1 NM_001006665 1 1 + 68 + 0 37 1 0 CGCGAATGCCGCGGCCCAGGATCAGCCAGACCT 572 CTCTGCCTGTCCACGCGTGGCGGATGT RPS6KA1 NM_001006665 1 1 + 103 − 0 37 1 0 CGCGAATGCCCACCGAGTCCCGCTGGGGGCCAG 573 AGCCAGGGCCAGACGCGTGGCGGATGT GUCY2F NM_001522 16 X − −28 + 0 37 1 0 CGCGAATGCCTTCTTCCTCTTCCTCTCACTCTCTG 574 CAGGCATCGAACGCGTGGCGGATGT GUCY2F NM_001522 16 X − 7 − 0 37 1 0 CGCGAATGCCGCAAAGTAAGGCAGACCATCATG 575 GCAAAGGCAGGGACGCGTGGCGGATGT GUCY2F NM_001522 16 X − 42 + 0 37 1 0 CGCGAATGCCTTATAGCCCTGCTGTCTATTAATG 576 GATTTGCTTACACGCGTGGCGGATGT GUCY2F NM_001522 16 X − 77 − 0 37 1 0 CGCGAATGCCTATATACAAAAATAACAAAAAAT 577 GTACCTTATAAAACGCGTGGCGGATGT RBBP8 NM_002894 5 18 + −14 + 0 37 1 0 CGCGAATGCCGTTTTGTTTCATAGGTTAAGAGC 578 AGGCTTATGTGAACGCGTGGCGGATGT RBBP8 NM_002894 5 18 + 21 − 0 37 1 0 CGCGAATGCCTTTTCCGCATATGTTCTTCAGTTA 579 CTGCACAGCGAACGCGTGGCGGATGT RBBP8 NM_002894 5 18 + 56 + 0 37 1 0 CGCGAATGCCAACAGCAAGAGTTTGAAAATATC 580 CGGCAGCAGAATACGCGTGGCGGATGT RBBP8 NM_002894 5 18 + 91 − 0 37 1 0 CGCGAATGCCAAAGGAAACTCACTAAGTTCTGT 581 AATAAGTTTAAGACGCGTGGCGGATGT KSR2 NM_173598 3 12 − −5 + 0 37 1 0 CGCGAATGCCAACAGCACAATCTGGTATGAACT 582 CCACGCCAGGGAACGCGTGGCGGATGT KSR2 NM_173598 3 12 − 30 − 0 37 1 0 CGCGAATGCCTTATTGCCTCTGCTGGTTGGGTCT 583 TGAAAGGCCATACGCGTGGCGGATGT KSR2 NM_173598 3 12 − 65 + 0 37 1 0 CGCGAATGCCTCTGGCAAATGGGCACAGGCATG 584 AAACCCAACCTCACGCGTGGCGGATGT KSR2 NM_173598 3 12 − 100 − 0 37 1 0 CGCGAATGCCCTTACCGAGATTTCTTTTCCCATG 585 CCAATCTGGCTACGCGTGGCGGATGT EPHA7 NM_004440 2 6 − 0 + 0 37 1 0 CGCGAATGCCGCCAATAAGCCCTCTTCTGGATC 586 AAAACACTCCTGACGCGTGGCGGATGT EPHA7 NM_004440 2 6 − 35 − 0 37 1 0 CGCGAATGCCTAGCCATTCTCCAACTGAACAAA 587 AGGTAGTGAAATACGCGTGGCGGATGT EPHA7 NM_004440 2 6 − 70 + 0 37 1 0 CGCGAATGCCCAAGCTATTAAGATGGAAAGATA 588 TAAAGATAATTTACGCGTGGCGGATGT EPHA7 NM_004440 2 6 − 105 − 0 37 1 0 CGCGAATGCCCTACTGATTCAAGGGAATTGTAG 589 CCAGCTGCCGTGACGCGTGGCGGATGT EPHA7 NM_004440 2 6 − 140 + 0 37 1 0 CGCGAATGCCCCAGGATGACTATTGAGTAAGCT 590 TAAACTCTTAAAACGCGTGGCGGATGT RB1 NM_000321 24 13 + −54 + 0 37 1 0 CGCGAATGCCCAAAATTGTATATGGTTTTTTATT 591 ACTAATTGGTAACGCGTGGCGGATGT RB1 NM_000321 24 13 + −19 − 0 37 1 0 CGCGAATGCCAATTGATACTAAGATTCTGTCAA 592 GTTAAGATGAAAACGCGTGGCGGATGT RB1 NM_000321 24 13 + 16 + 0 37 1 0 CGCGAATGCCGGTGAATCATTCGGGGTGAGTAT 593 TTTCTTTCTATGACGCGTGGCGGATGT RB1 NM_000321 24 13 + 51 − 0 37 1 0 CGCGAATGCCTTTCTTTTATACTTACAATGCATA 594 CTATTATATTTACGCGTGGCGGATGT EPHA4 NM_004438 11 2 − −14 + 0 37 1 0 CGCGAATGCCATTGTCATTCCCAGTGCCTTCCCG 595 GATCATTGGAGACGCGTGGCGGATGT EPHA4 NM_004438 11 2 − 21 − 0 37 1 0 CGCGAATGCCGACAGAGACCAGAAGGACTGTG 596 GAGTTAGCCCCATACGCGTGGCGGATGT EPHA4 NM_004438 11 2 − 56 + 0 37 1 0 CGCGAATGCCTCGGGCAGTGTGGTGCTGGTGGT 597 AATTCTCATTGCACGCGTGGCGGATGT EPHA4 NM_004438 11 2 − 91 − 0 37 1 0 CGCGAATGCCGCTTCAGTGCTTACCTCCGGCTG 598 ATGACAAAAGCTACGCGTGGCGGATGT EPHB1 NM_004441 12 3 + 0 + 0 37 1 0 CGCGAATGCCCAAAATGACGGGCAGTTCACCGT 599 GATCCAGCTTGTACGCGTGGCGGATGT EPHB1 NM_004441 12 3 + 35 − 0 37 1 0 CGCGAATGCCACTTCATGCCAGCAGCGATGCCC 600 CTGAGCATACCCACGCGTGGCGGATGT EPHB1 NM_004441 12 3 + 70 + 0 37 1 0 CGCGAATGCCACCTGGCTGAGATGAATTATGTG 601 CATCGGGACCTGACGCGTGGCGGATGT EPHB1 NM_004441 12 3 + 105 − 0 37 1 0 CGCGAATGCCACCAGGTTACTGTTGACCAGAAT 602 GTTCCTAGCAGCACGCGTGGCGGATGT EPHB1 NM_004441 12 3 + 140 + 0 37 1 0 CGCGAATGCCGTGCAAGGTGTCCGACTTTGGCC 603 TCTCCCGCTACCACGCGTGGCGGATGT EPHB1 NM_004441 12 3 + 175 − 0 37 1 0 CGCGAATGCCGCTGGTGTAGGTGGGATCTGAGG 604 TGTCATCCTGGAACGCGTGGCGGATGT EPHB1 NM_004441 12 3 + 210 + 0 37 1 0 CGCGAATGCCTCCTTGGTGAGTCCTTCTTGGCAT 605 TCTCAAGTAGAACGCGTGGCGGATGT RB1 NM_000321 26 13 + −45 + 0 37 1 0 CGCGAATGCCATTTATAAATACACATGAAATGT 606 TTTGCATTTTTTACGCGTGGCGGATGT RB1 NM_000321 26 13 + −10 − 0 37 1 0 CGCGAATGCCTTTGGACTCTCCTGGGAGATGTTT 607 ACTGCAGATTAACGCGTGGCGGATGT RB1 NM_000321 26 13 + 25 + 0 37 1 0 CGCGAATGCCTTTCAGCAGAAACTGGCAGAAAT 608 GAGTAAGTACTTACGCGTGGCGGATGT RB1 NM_000321 26 13 + 60 − 0 37 1 0 CGCGAATGCCAAACAATTGTTTATTTCATTTACA 609 CAAGGTGAAAAACGCGTGGCGGATGT CHAF1A NM_005483 12 19 + 0 + 0 37 1 0 CGCGAATGCCGAGTGTGCCGACCCTGAGAACCA 610 TAAGGTCCGCCAACGCGTGGCGGATGT CHAF1A NM_005483 12 19 + 35 − 0 37 1 0 CGCGAATGCCCCAGGAACTCGTCCCACTCCTTG 611 GCCTTCAGTTTCACGCGTGGCGGATGT CHAF1A NM_005483 12 19 + 70 + 0 37 1 0 CGCGAATGCCCTAAGGGGAAGCGCTTTCGCGTC 612 CTGCAACCTGTGACGCGTGGCGGATGT CHAF1A NM_005483 12 19 + 105 − 0 37 1 0 CGCGAATGCCCAGTCTCTGTCAGCCGCCCACAC 613 GCAGCCGATCTTACGCGTGGCGGATGT CHAF1A NM_005483 12 19 + 140 + 0 37 1 0 CGCGAATGCCCGCAGGCGATGACCTGAAGGTAC 614 TGCAGCAGTTCGACGCGTGGCGGATGT CHAF1A NM_005483 12 19 + 175 − 0 37 1 0 CGCGAATGCCCTCCTGGGCCGGCAGGGTCTCCA 615 GGAAGCAGGCTGACGCGTGGCGGATGT CHAF1A NM_005483 12 19 + 210 + 0 37 1 0 CGCGAATGCCGAGCAGACGCCCAAGGCCTCCAA 616 GCGGGAGAGGAGACGCGTGGCGGATGT CHAF1A NM_005483 12 19 + 245 − 0 37 1 0 CGCGAATGCCTGGCCTGGCCCCGCCCACACTCA 617 CTCTGCTCGTCTACGCGTGGCGGATGT EPHA3 NM_005233 10 3 + −7 + 0 37 1 0 CGCGAATGCCCAAACAGTAAAACTTCCAGGTCT 618 CAGGACTTATGTACGCGTGGCGGATGT EPHA3 NM_005233 10 3 + 28 − 0 37 1 0 CGCGAATGCCCAGCTTGGGTAGGGTCTTCATAT 619 GTATGTGGGTCAACGCGTGGCGGATGT EPHA3 NM_005233 10 3 + 63 + 0 37 1 0 CGCGAATGCCTTCATGAGTTTGCCAAGGAATTG 620 GATGCCACCAACACGCGTGGCGGATGT EPHA3 NM_005233 10 3 + 98 − 0 37 1 0 CGCGAATGCCTGGTTACCTGCTCCAACAACTTTA 621 TCAATGGATATACGCGTGGCGGATGT EPHA3 NM_005233 4 3 + 0 + 0 37 1 0 CGCGAATGCCCTTGTCGACCAGGTTTCTACAAG 622 GCATTGGATGGTACGCGTGGCGGATGT EPHA3 NM_005233 4 3 + 35 − 0 37 1 0 CGCGAATGCCGAACTGTGAGGCGGGCACTTAGC 623 ACACTTCATATTACGCGTGGCGGATGT EPHA3 NM_005233 4 3 + 70 + 0 37 1 0 CGCGAATGCCTACTCAGGAAGATGGTTCAATGA 624 ACTGCAGGTGTGACGCGTGGCGGATGT EPHA3 NM_005233 4 3 + 105 − 0 37 1 0 CGCGAATGCCTGGAGGGTCTTTGTCTGCCCGGA 625 AGTAATTATTCTACGCGTGGCGGATGT EPHA3 NM_005233 4 3 + 140 + 0 37 1 0 CGCGAATGCCTCCATGGCTTGTACCCGTGAGTA 626 GTTTTGCTGCAAACGCGTGGCGGATGT RB1 NM_000321 10 13 + −15 + 0 37 1 0 CGCGAATGCCCTTTTTTCTTTCAAGGTTGAAAAT 627 CTTTCTAAACGACGCGTGGCGGATGT RB1 NM_000321 10 13 + 20 − 0 37 1 0 CGCGAATGCCCTAGATCTTTATTTTTAAGATAAA 628 TTTCTTCGTATACGCGTGGCGGATGT RB1 NM_000321 10 13 + 55 + 0 37 1 0 CGCGAATGCCATGCAAGATTATTTTTGGATCAT 629 GATAAAACTCTTACGCGTGGCGGATGT RB1 NM_000321 10 13 + 90 − 0 37 1 0 CGCGAATGCCTACCATGTGCAATACCTGTCTAT 630 AGAATCAGTCTGACGCGTGGCGGATGT PIK3CA NM_006218 15 3 + −16 + 0 37 1 0 CGCGAATGCCCTTTTTTTTTAATCAGGTACAGAT 631 GAAGTTTTTAGACGCGTGGCGGATGT PIK3CA NM_006218 15 3 + 19 − 0 37 1 0 CGCGAATGCCAGCATCCATGAAATCTGGTCGCC 632 TCATTTGCTCAAACGCGTGGCGGATGT PIK3CA NM_006218 15 3 + 54 + 0 37 1 0 CGCGAATGCCCTACAGGGCTTTCTGTCTCCTCTA 633 AACCCTGCTCAACGCGTGGCGGATGT PIK3CA NM_006218 15 3 + 89 − 0 37 1 0 CGCGAATGCCAACCCCCAAGAAAGTACCTGAGG 634 TTTCCTAGTTGAACGCGTGGCGGATGT EPHB1 NM_004441 8 3 + −16 + 0 37 1 0 CGCGAATGCCCCTCTTTCTCACCTAGATGATTAC 635 AAGTCAGAGCTACGCGTGGCGGATGT EPHB1 NM_004441 8 3 + 19 − 0 37 1 0 CGCGAATGCCCTGCCGAGCCAGCAATCAGGGGC 636 AGCTGCTCCCTCACGCGTGGCGGATGT EPHB1 NM_004441 8 3 + 54 + 0 37 1 0 CGCGAATGCCCGGCCGGGGTCGTGTTCGTTGTG 637 TCCTTGGTGGCCACGCGTGGCGGATGT EPHB1 NM_004441 8 3 + 89 − 0 37 1 0 CGCGAATGCCGAGTGGAGGACCTACCTGCTACA 638 GACGATAGAGATACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 0 + 0 37 1 0 CGCGAATGCCTGGGAAGAATTAGTGGTTTGGA 639 TGAGAACTATACACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 35 − 0 37 1 0 CGCGAATGCCCCATGACTTGGCACACCTGGTAT 640 GTTCGTATCGGGACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 70 + 0 37 1 0 CGCGAATGCCAGCCCAACCAAAACAACTGGCTG 641 CGGACTAACTGGACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 105 − 0 37 1 0 CGCGAATGCCTCTACAAAAATCCTTTGTGCATTG 642 CCTTTGGAAATACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 140 + 0 37 1 0 CGCGAATGCCATTGAAATTCACCCTGAGGGATT 643 GTAACAGTCTTCACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 175 − 0 37 1 0 CGCGAATGCCATTAAATGTTTCCTTGCAAGTTCC 644 CAGTACTCCAGACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 210 + 0 37 1 0 CGCGAATGCCTTGTACTATTATGAAACAGACTA 645 TGACACTGGCAGACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 245 − 0 37 1 0 CGCGAATGCCTGTCTATTTTTACATAGAGGTTTT 646 CTCTTATATTCACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 280 + 0 37 1 0 CGCGAATGCCCCATTGCTGCAGATGAAAGTTTT 647 ACCCAAGGTGACACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 315 − 0 37 1 0 CGCGAATGCCACCTCAGTGTTAAGCTTCATCTTT 648 CTTTCACCAAGACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 350 + 0 37 1 0 CGCGAATGCCGAGAGAGATTGGACCTTTGTCCA 649 AAAAGGGATTCTACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 385 − 0 37 1 0 CGCGAATGCCAGCTATGCAAGCCCCTACATCCT 650 GAAAGGCAAGATACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 420 + 0 37 1 0 CGCGAATGCCTTGGTTTCTGTCAAAGTGTACTAC 651 AAGAAGTGCTGACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 455 − 0 37 1 0 CGCGAATGCCTATCTGGAAAGATAGCTAAGTTC 652 TCAATAATGGACACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 490 + 0 37 1 0 CGCGAATGCCCAGTGACTGGTTCAGAATTTTCCT 653 CTTTAGTCGAGACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 525 − 0 37 1 0 CGCGAATGCCTCTTCCTCTGCACTGCTGACACAT 654 GTCCCTCGAACACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 560 + 0 37 1 0 CGCGAATGCCAGCGGAAAACGCCCCCAGGATGC 655 ACTGCAGTGCAGACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 595 − 0 37 1 0 CGCGAATGCCGATACATTTTCCAATGGGCACTA 656 ACCATTCTCCTTACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 630 + 0 37 1 0 CGCGAATGCCTGCAAAGCAGGCTACCAGCAAAA 657 AGGAGACACTTGACGCGTGGCGGATGT EPHA7 NM_004440 15 6 − 665 − 0 37 1 0 CGCGAATGCCAGTGGAGTTTTAATAGGACACAT 658 TACTTACGTTCAACGCGTGGCGGATGT PALB2 NM_024675 8 16 − −34 + 0 37 1 0 CGCGAATGCCGTGTAGACTAATGATGTGACTTT 659 TGTTTTCACAGAACGCGTGGCGGATGT PALB2 NM_024675 8 16 − 1 − 0 37 1 0 CGCGAATGCCTATGCTATCAGAAGCAGGAAGCT 660 CTGCTGTTTCAGACGCGTGGCGGATGT PALB2 NM_024675 8 16 − 36 + 0 37 1 0 CGCGAATGCCAACCCAGGCAACCTACAATTGGT 661 TTCAGAGTTAAAACGCGTGGCGGATGT PALB2 NM_024675 8 16 − 71 − 0 37 1 0 CGCGAATGCCGACACGAGACACTGGAAGAGAA 662 TATTCTTCTGACCACGCGTGGCGGATGT RET NM_020630 3 10 + 0 + 0 37 1 0 CGCGAATGCCACCGCGGCTTTCCCCTGCTCACC 663 GTCTACCTCAAGACGCGTGGCGGATGT RET NM_020630 3 10 + 35 − 0 37 1 0 CGCGAATGCCTCGCCCTCACGAAGGGATGTGGG 664 TGACAGGAAGACACGCGTGGCGGATGT RET NM_020630 3 10 + 70 + 0 37 1 0 CGCGAATGCCGTGCCAGTGGCCAGGCTGTGCCC 665 GCGTATACTTCTACGCGTGGCGGATGT RET NM_020630 3 10 + 105 − 0 37 1 0 CGCGAATGCCGGAGCTGCAGGCTGGAAAGGAG 666 GTGTTGAAGAAGGACGCGTGGCGGATGT RET NM_020630 3 10 + 140 + 0 37 1 0 CGCGAATGCCCTCAAGCCCCGGGAGCTCTGCTT 667 CCCAGAGACAAGACGCGTGGCGGATGT RET NM_020630 3 10 + 175 − 0 37 1 0 CGCGAATGCCCTGGGGGTCGGTTCTCCCGAATG 668 CGGAAGGAGGGCACGCGTGGCGGATGT RET NM_020630 3 10 + 210 + 0 37 1 0 CGCGAATGCCGCACCTTCCACCAGTTCCGCCTG 669 CTGCCTGTGCAGACGCGTGGCGGATGT RET NM_020630 3 10 + 245 − 0 37 1 0 CGCGAATGCCAGCCTGTAGGCCACGCTGATGTT 670 GGGGCACAAGAAACGCGTGGCGGATGT RET NM_020630 3 10 + 280 + 0 37 1 0 CGCGAATGCCCCTGGAGGGTGAGTGCCGACCTT 671 GTGGGGCCGCCCACGCGTGGCGGATGT NTRK3 NM_001012338 4 15 − 0 + 0 37 1 0 CGCGAATGCCGGCCCATGGGCCAGATGCAATGA 672 TCCTTGTGGATGACGCGTGGCGGATGT NTRK3 NM_001012338 4 15 − 35 − 0 37 1 0 CGCGAATGCCGAGCCCCAGCTCACCCTTGGCCT 673 GGCGTGGCTGTCACGCGTGGCGGATGT NTRK3 NM_001012338 4 15 − 70 + 0 37 1 0 CGCGAATGCCTCCCAAATGCTCCACATTGCCAG 674 TCAGATCGCCTCACGCGTGGCGGATGT NTRK3 NM_001012338 4 15 − 105 − 0 37 1 0 CGCGAATGCCGCACAAAGTGCTGGGAGGCCAG 675 GTACACCATACCCACGCGTGGCGGATGT NTRK3 NM_001012338 4 15 − 140 + 0 37 1 0 CGCGAATGCCACCGAGACCTGGCCACCAGGAAC 676 TGCCTGGTTGGAACGCGTGGCGGATGT NTRK3 NM_001012338 4 15 − 175 − 0 37 1 0 CGCGAATGCCATGCCGAAGTCCCCAATCTTCAC 677 TAGCAGATTCGCACGCGTGGCGGATGT NTRK3 NM_001012338 4 15 − 210 + 0 37 1 0 CGCGAATGCCGTCCAGAGATGTCTACAGCACGG 678 ATTATTACAGGGACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 0 + 0 37 1 0 CGCGAATGCCGGAAGAAAAGTCGTCATCAAAA 679 AGAGGATTCCCTTACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 35 − 0 37 1 0 CGCGAATGCCTCATCCAAGGATAAATAAGCACT 680 ATTACTCCAAGAACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 70 + 0 37 1 0 CGCGAATGCCTGATGCTTTCACGGCTCCATTTCA 681 TAGGGATGGAAACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 105 − 0 37 1 0 CGCGAATGCCACTGAGAAAAGACAGTAGTTGCT 682 TTAAACTCAGCAACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 140 + 0 37 1 0 CGCGAATGCCATCACAGACTTTCAGTTACCTGA 683 TGAAGACTTTGGACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 175 − 0 37 1 0 CGCGAATGCCCTGAGCAGGACTTCACTTTTTCA 684 AGCTTAAGAGGTACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 210 + 0 37 1 0 CGCGAATGCCAAAAACCAGTGGAGCCCTTTGAG 685 TCAAAAATGTTTACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 245 − 0 37 1 0 CGCGAATGCCAAAATACAGCTTCCCTCTTTAAG 686 ATGTCTCTCTCCACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 280 + 0 37 1 0 CGCGAATGCCTCCAGAGGAACTGAGTCCTAAAC 687 GCATGGATACAGACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 315 − 0 37 1 0 CGCGAATGCCTAGAACAATAAGGTCCTCTTCTA 688 AGTCCTCCATTTACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 350 + 0 37 1 0 CGCGAATGCCCCAGGAAAATCACATCCCAAAAG 689 GCCAAACTCGCAACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 385 − 0 37 1 0 CGCGAATGCCTGGATGAAGAAAGGCCCGTCTTT 690 GTATGCTGGCTTACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 420 + 0 37 1 0 CGCGAATGCCTATTACTTTATACTCCTTTAAATA 691 CGGTTGCGCCTACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 455 − 0 37 1 0 CGCGAATGCCGAACACATGTCTGTGGTAGGCCT 692 GTCATTATCATCACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 490 + 0 37 1 0 CGCGAATGCCACCTGCTTTCCCCATCTTAGGTAC 693 TACTCCAGCCTACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 525 − 0 37 1 0 CGCGAATGCCTGTAGATGCTTTTTCATAGGAGC 694 CTTGAGGGCCAAACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 560 + 0 37 1 0 CGCGAATGCCGAAGTTGCTGGACGAACTTGCTG 695 CACACCCCAACTACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 595 − 0 37 1 0 CGCGAATGCCCACTGGCAAGACAGACTGAGTCT 696 TTCAAATGAGCAACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 630 + 0 37 1 0 CGCGAATGCCATACTAAACAATTCGACAGTTCA 697 GGCAGCCCAGCAACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 665 − 0 37 1 0 CGCGAATGCCTGCCTGCCTGACACTTGCAGGGT 698 GGTATGTGGTTTACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 700 + 0 37 1 0 CGCGAATGCCAGGACAACCTACCTGTGACTGTG 699 ACTCTGTCCCGCACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 735 − 0 37 1 0 CGCGAATGCCAAAAGTGAATGACTCAATGGGTG 700 GAGGTGTTCCTGACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 770 + 0 37 1 0 CGCGAATGCCAAAGAAAATCAGCTCTGTAGAAA 701 CACATGCCAGGAACGCGTGGCGGATGT PALB2 NM_024675 9 16 − 805 − 0 37 1 0 CGCGAATGCCTGGATTGTACCTGTTCGACGGAA 702 TGTTTATGCAGCACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 0 + 0 37 1 0 CGCGAATGCCGTCACCTTCCGCAACCCTGTCATT 703 GAGAGGATTCCACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 35 − 0 37 1 0 CGCGAATGCCGCTTGGAGAAAATTTTCTTCTGCC 704 GTCGGAGCCGAACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 70 + 0 37 1 0 CGCGAATGCCAGCAAGGTGAGAGGGTGCTCCAG 705 GCTTCCTGGGGGACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 105 − 0 37 1 0 CGCGAATGCCCACCCTCCATGACACTCCCAGGA 706 CCCCTGGCCTCTACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 140 + 0 37 1 0 CGCGAATGCCACGCCTGCCCCGTCCCCCCAGGG 707 AAGGCGTTCCAGACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 175 − 0 37 1 0 CGCGAATGCCCACGTGGCGACATCGATGTTCAT 708 CTGCCTAGCACGACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 210 + 0 37 1 0 CGCGAATGCCGGTGCGGCTGCTCCGGAGGCTCA 709 TCCCCAATGCCAACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 245 − 0 37 1 0 CGCGAATGCCTGGAGAAGCCCCAGGGCTAAAG 710 GTGCCTGTGCCCGACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 280 + 0 37 1 0 CGCGAATGCCGGATCCGAGGCCCGGACCACGGG 711 GTAAGGAAGGAGACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 315 − 0 37 1 0 CGCGAATGCCCGCAGGCTTCAAGGGGCAGCCGG 712 GACCATGGGGCCACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 350 + 0 37 1 0 CGCGAATGCCAGGGTCCTGGGTCCCAGACACAC 713 CCTCCTCTCGTCACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 385 − 0 37 1 0 CGCGAATGCCAGGTTCAGCTTCTCCACCGATAT 714 GTCACTGCAGCAACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 420 + 0 37 1 0 CGCGAATGCCCGGCACTGACTCGGACAGCTCAC 715 CTCAGAAGAGCTACGCGTGGCGGATGT PKN1 NM_002741 12 19 + 455 − 0 37 1 0 CGCGAATGCCCACCAGGCTCGATGGGCTGGAAG 716 GAGGATCCCGCGACGCGTGGCGGATGT NTRK3 NM_001012338 7 15 − 0 + 0 37 1 0 CGCGAATGCCGTCCCGTGGCTGTCATCAGTGGT 717 GAGGAGGACTCAACGCGTGGCGGATGT NTRK3 NM_001012338 7 15 − 35 − 0 37 1 0 CGCGAATGCCGTGATGCCGTGGTTGATGTGGTG 718 CAGTGGGCTGGCACGCGTGGCGGATGT NTRK3 NM_001012338 7 15 − 70 + 0 37 1 0 CGCGAATGCCCACGCCCTCGTCACTGGATGCCG 719 GGCCCGACACTGACGCGTGGCGGATGT NTRK3 NM_001012338 7 15 − 105 − 0 37 1 0 CGCGAATGCCCTCAATGACAGGGATGCGAGTCA 720 TGCCAATGACCAACGCGTGGCGGATGT NTRK3 NM_001012338 7 15 − 140 + 0 37 1 0 CGCGAATGCCAACCCCCAGTACTTCCGTCAGGG 721 ACACAACTGCCAACGCGTGGCGGATGT NTRK3 NM_001012338 7 15 − 175 − 0 37 1 0 CGCGAATGCCGCATTCCATCCCAGTACTTACAC 722 GTGTCCGGCTTGACGCGTGGCGGATGT RPS6KA1 NM_002953 14 1 + −4 + 0 37 1 0 CGCGAATGCCACAGATTCCCCAGGCATCCCCCC 723 CAGCGCTGGGGCACGCGTGGCGGATGT RPS6KA1 NM_002953 14 1 + 31 − 0 37 1 0 CGCGAATGCCTGGCCACGAAGCTGAAGCCCCGG 724 AACAGCTGATGGACGCGTGGCGGATGT RPS6KA1 NM_002953 14 1 + 66 + 0 37 1 0 CGCGAATGCCCCGGCCTGATGGAAGACGACGGC 725 AAGCCTCGTGCCACGCGTGGCGGATGT RPS6KA1 NM_002953 14 1 + 101 − 0 37 1 0 CGCGAATGCCCTCACCTGTACCACCGAGTGCAG 726 GGGTGCCTGCGGACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 0 + 0 37 1 0 CGCGAATGCCATGTTCCTGGGACTCGGGCGCTT 727 TTCTCGCCTTGTACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 35 − 0 37 1 0 CGCGAATGCCGTCCCAGCAGTTTCCTGAAAGCC 728 GCAAACCAGAGAACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 70 + 0 37 1 0 CGCGAATGCCACCATGGCCTTGCATCTGCCAAG 729 TTCCTGTGGTGCACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 105 − 0 37 1 0 CGCGAATGCCTGCTGCGGAAGGGACATGACAGA 730 CAGAAGGCACAAACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 140 + 0 37 1 0 CGCGAATGCCGGTGTGGACACTCCCCTACAAGA 731 TAGGGGTGGTGGACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 175 − 0 37 1 0 CGCGAATGCCGGCCTTTGAAAACAGCGAATCAC 732 AAGCCCAAGGGCACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 210 + 0 37 1 0 CGCGAATGCCCTGCCTGAGGTTGCTGCGCGATT 733 AGCCATTGAGCGACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 245 − 0 37 1 0 CGCGAATGCCAATAACTCAGGTCAAAAGATGGG 734 TCCCGGTTGATTACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 280 + 0 37 1 0 CGCGAATGCCCTTTTGAATACGTGATTCTCAATG 735 AAGACTGCCAGACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 315 − 0 37 1 0 CGCGAATGCCTGGTGGGAAATGAAACTGGAGA 736 GAGCCCTCGAAGTACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 350 + 0 37 1 0 CGCGAATGCCCCAGATGGCCTCAGGATTTATTG 737 GACCTACCAACCACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 385 − 0 37 1 0 CGCGAATGCCGTTTCCCAGGAGCGAGGCTGCCT 738 CGCAGTAGCCAGACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 420 + 0 37 1 0 CGCGAATGCCAGCTGGGACAAAGGAATTTTCTC 739 TTGGGCTTGTGTACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 455 − 0 37 1 0 CGCGAATGCCTCGGGTAGCTAATTTTATTGTCTA 740 ATTCATAATTCACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 490 + 0 37 1 0 CGCGAATGCCCCTTTTCTCGGACACTCCCTTCTC 741 CCATCCGGGTGACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 525 − 0 37 1 0 CGCGAATGCCTGAGCCCACTGGAAATATTTCAT 742 GACAGTTACAAGACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 560 + 0 37 1 0 CGCGAATGCCTGCTGGAGTCATTTCCTCAGATG 743 AAGACATTTGGGACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 595 − 0 37 1 0 CGCGAATGCCCCGAAGAGCACTTGCGACTCGAT 744 TGGCTGTATGCAACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 630 + 0 37 1 0 CGCGAATGCCAGCCACGGCTTACCTGTAGGGGT 745 CGTCCTGACCACACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 665 − 0 37 1 0 CGCGAATGCCGGAGGGCTTTCCGCATGCTTTGG 746 CTGTCTTGTCCTACGCGTGGCGGATGT GUCY2F NM_001522 19 X − 700 + 0 37 1 0 CGCGAATGCCAGAGGATTCACCAGGCAGACAG 747 AATTCGCAGTGAGACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 0 + 0 37 1 0 CGCGAATGCCGCTAGTAACGGGGGCCACACTAG 748 CGACTACTCTTCACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 35 − 0 37 1 0 CGCGAATGCCCCCGGTGACCAACATTCGGTGAG 749 GACGGGAGGGAAACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 70 + 0 37 1 0 CGCGAATGCCAGCTCCGAGCCGAGGCAGCTGCA 750 GTGGCTGGATTGACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 105 − 0 37 1 0 CGCGAATGCCCGCTTGGCTGCCCGGTGCAGGGA 751 CCCTGGGGTGCTACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 140 + 0 37 1 0 CGCGAATGCCCAGGACCAGCCTTTTTGCGGTAT 752 TGGACATGGACAACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 175 − 0 37 1 0 CGCGAATGCCGCAGGTCATGGGGGACAGGAGG 753 ATGGGGGTCAGCAACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 210 + 0 37 1 0 CGCGAATGCCCCATCTTCCCTAGTCCCTAGACTC 754 CTCTGTTGGGCACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 245 − 0 37 1 0 CGCGAATGCCACTGATGTGACGATAGGGAAGAT 755 AGATGAAGAAGGACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 280 + 0 37 1 0 CGCGAATGCCGTATCCCTCTATCCATCCTCAAAC 756 TGATTCCAATAACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 315 − 0 37 1 0 CGCGAATGCCTCTCGGAGTCACTACCCCGACGA 757 TTCTGGAATGGTACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 350 + 0 37 1 0 CGCGAATGCCAACGAAGCTTGGATAGTCGGGGA 758 GAGACAACAGGGACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 385 − 0 37 1 0 CGCGAATGCCTCACTCACCTGTTTGATGGGGAT 759 GGCTCGCCCACTACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 420 + 0 37 1 0 CGCGAATGCCTCCAGGGGCTGGGACAGGCTAGG 760 GGCAGCTGGTCTACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 455 − 0 37 1 0 CGCGAATGCCAAGGGGAAAGACACATGTGGGA 761 AAAAAGCCAACTAACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 490 + 0 37 1 0 CGCGAATGCCTCTCCAGAGCTTCCTACTAAAAC 762 GAAGTGGCAATTACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 525 − 0 37 1 0 CGCGAATGCCGGTTACATATTTCTTCTTCCATTC 763 TTTGTTCAAGGACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 560 + 0 37 1 0 CGCGAATGCCCTGTCCAGTAATGGCTTTCTACTC 764 TACCACCCCAGACGCGTGGCGGATGT CENTG1 NM_014770 11 12 − 595 − 0 37 1 0 CGCGAATGCCCCAATGTGGCCCAGTCTCTGCCA 765 CTCACGTTAATAACGCGTGGCGGATGT RBBP8 NM_002894 8 18 + −18 + 0 37 1 0 CGCGAATGCCATTTTTTTCTCCCCTTAGAAATGA 766 GAAAAGTTTCCACGCGTGGCGGATGT RBBP8 NM_002894 8 18 + 17 − 0 37 1 0 CGCGAATGCCTCATTAGGATTATGTTGTGGATG 767 AGTTGAAGACTTACGCGTGGCGGATGT RBBP8 NM_002894 8 18 + 52 + 0 37 1 0 CGCGAATGCCAAATGAAATTCTAGTAGCTGACA 768 CTTATGACCAAAACGCGTGGCGGATGT RBBP8 NM_002894 8 18 + 87 − 0 37 1 0 CGCGAATGCCCTCAGTATCTTGCTTACTGGCCAT 769 TGGAGATTGACACGCGTGGCGGATGT NFKB1 NM_003998 19 4 + −18 + 0 37 1 0 CGCGAATGCCGTGTGGGCTGGATTGTAGGGTGA 770 TGCCCATGTGGAACGCGTGGCGGATGT NFKB1 NM_003998 19 4 + 17 − 0 37 1 0 CGCGAATGCCTATGCAGGGGTGTGGTTCCATCG 771 TAGGTAGTACTGACGCGTGGCGGATGT NFKB1 NM_003998 19 4 + 52 + 0 37 1 0 CGCGAATGCCTAGCAGCTGGGAGAGGGTCCACC 772 AGGCTGGCAGCTACGCGTGGCGGATGT NFKB1 NM_003998 19 4 + 87 − 0 37 1 0 CGCGAATGCCCGCCAGATCACCATCTTACCTGC 773 TGCTTTGAGAAGACGCGTGGCGGATGT GUCY2F NM_001522 6 X − 0 + 0 37 1 0 CGCGAATGCCAGACCATTGGAGATGCCTACATG 774 GTGGCTTCAGGCACGCGTGGCGGATGT GUCY2F NM_001522 6 X − 35 − 0 37 1 0 CGCGAATGCCTCAGCTGCATGCCTACTGCCATTC 775 CTCTTTGGGAGACGCGTGGCGGATGT GUCY2F NM_001522 6 X − 70 + 0 37 1 0 CGCGAATGCCGATTGCAAACATGTCCTTAGATA 776 TCCTGAGCTCTGACGCGTGGCGGATGT GUCY2F NM_001522 6 X − 105 − 0 37 1 0 CGCGAATGCCCACTTCTGGCATGTGCCGCATCTT 777 GAAAGTGCCCAACGCGTGGCGGATGT GUCY2F NM_001522 6 X − 140 + 0 37 1 0 CGCGAATGCCCCGGTCCGAATTCGAATTGGCCT 778 TCACTCAGGTAAACGCGTGGCGGATGT RB1 NM_000321 3 13 + −12 + 0 37 1 0 CGCGAATGCCTTTTGTTCCCAGGGAGGTTATATT 779 CAAAAGAAAAAACGCGTGGCGGATGT RB1 NM_000321 3 13 + 23 − 0 37 1 0 CGCGAATGCCCTGCTGCAATAAAGATACAGATT 780 CCCCACAGTTCCACGCGTGGCGGATGT RB1 NM_000321 3 13 + 58 + 0 37 1 0 CGCGAATGCCTTGACCTAGATGAGATGTCGTTC 781 ACTTTTACTGAGACGCGTGGCGGATGT RB1 NM_000321 3 13 + 93 − 0 37 1 0 CGCGAATGCCAAGAAACTTTACCTGATTTCTAT 782 GTTTTTCTGTAGACGCGTGGCGGATGT RPS6KA1 NM_002953 8 1 + −51 + 0 37 1 0 CGCGAATGCCCACCCACACGGCCACAGCTGAGG 783 GGCCCTGACCACACGCGTGGCGGATGT RPS6KA1 NM_002953 8 1 + −16 − 0 37 1 0 CGCGAATGCCCTCCTCATCCAGAAGGATGCTGT 784 AATAGAGAAATAACGCGTGGCGGATGT RPS6KA1 NM_002953 8 1 + 19 + 0 37 1 0 CGCGAATGCCGGCCACATCAAACTCACTGGTGA 785 GTGGAGGGCGCCACGCGTGGCGGATGT RPS6KA1 NM_002953 8 1 + 54 − 0 37 1 0 CGCGAATGCCCCTTGTCCTGTCCTCCCCTGGGTC 786 CCGAGGGGGCAACGCGTGGCGGATGT RBBP8 NM_002894 14 18 + −8 + 7 37 1 0 CGCGAATGCCTTATTTAGGATGGCAGTCAGTCA 787 AAATTAGGAGGAACGCGTGGCGGATGT RBBP8 NM_002894 14 18 + 23 − 0 37 1 0 CGCGAATGCCTAACCAATGTACAGTCCATGTCC 788 ACTGTCTCTCCTACGCGTGGCGGATGT RBBP8 NM_002894 14 18 + 58 + 0 37 1 0 CGCGAATGCCGTGAAACCGTTCTCTTAAAAATG 789 AAGAAGCAAGAGACGCGTGGCGGATGT RBBP8 NM_002894 14 18 + 93 − 0 37 1 0 CGCGAATGCCAAACAGATCTTACTTGAACTTTTT 790 TCTCCCTTCTGACGCGTGGCGGATGT KSR2 NM_173598 4 12 − −4 + 0 37 1 0 CGCGAATGCCCCAGGCGGGAGGACAAACTGCG 791 CATCCAGAATGGCACGCGTGGCGGATGT KSR2 NM_173598 4 12 − 31 − 0 37 1 0 CGCGAATGCCTGGCGGATGATCTCTGGTGCCAG 792 GTGGCATAGCCAACGCGTGGCGGATGT KSR2 NM_173598 4 12 − 66 + 0 37 1 0 CGCGAATGCCGCTGTCCCCCGACACAGAGGAGG 793 ATAAGCTCCCCTACGCGTGGCGGATGT KSR2 NM_173598 4 12 − 101 − 0 37 1 0 CGCGAATGCCTTACCCAAGGGCAAAGACGTCAG 794 AGTGCTTGGAGAACGCGTGGCGGATGT PIK3CA NM_006218 3 3 + 0 + 0 37 1 0 CGCGAATGCCGTTTTGCTATCGGCATGCCAGTGT 795 GTGAATTTGATACGCGTGGCGGATGT PIK3CA NM_006218 3 3 + 35 − 0 37 1 0 CGCGAATGCCCTTCGGAAGTCCTGTACTTCTGG 796 ATCTTTAACCATACGCGTGGCGGATGT PIK3CA NM_006218 3 3 + 70 + 0 37 1 0 CGCGAATGCCAAATATTCTGAACGTTTGTAAAG 797 AAGCTGTGGATCACGCGTGGCGGATGT PIK3CA NM_006218 3 3 + 105 − 0 37 1 0 CGCGAATGCCCATTGCTCTACTATGAGGTGAAT 798 TGAGGTCCCTAAACGCGTGGCGGATGT PIK3CA NM_006218 3 3 + 140 + 0 37 1 0 CGCGAATGCCTATGTCTATCCTCCAAATGTAGA 799 ATCTTCACCAGAACGCGTGGCGGATGT PIK3CA NM_006218 3 3 + 175 − 0 37 1 0 CGCGAATGCCCTTTATCTAATTTATTATATATGT 800 GCTTTGGCAATACGCGTGGCGGATGT PIK3CA NM_006218 3 3 + 210 + 0 37 1 0 CGCGAATGCCGTAAGAAAATGACTAATCTACTC 801 TAATCATTACTAACGCGTGGCGGATGT KSR2 NM_173598 6 12 − −22 + 0 37 1 0 CGCGAATGCCATCTTGTCCTTTTGCTTTTCAGCC 802 TCTGTAAGGGAACGCGTGGCGGATGT KSR2 NM_173598 6 12 − 13 − 0 37 1 0 CGCGAATGCCATTTTGGCATCCCTCACAACGGA 803 ATAGAGCGTCCGACGCGTGGCGGATGT KSR2 NM_173598 6 12 − 48 + 0 37 1 0 CGCGAATGCCCGTTTTGGATGTCAACAAAACCA 804 GGCAGATTGCTCACGCGTGGCGGATGT KSR2 NM_173598 6 12 − 83 − 0 37 1 0 CGCGAATGCCAGGCCCAAGAGCCGACAGTACCT 805 TCACAATTTCTTACGCGTGGCGGATGT PKN1 NM_002741 9 19 + 0 + 0 37 1 0 CGCGAATGCCGTGAAGTCAGCACTGTGCTTAAG 806 CTGGATAACACAACGCGTGGCGGATGT PKN1 NM_002741 9 19 + 35 − 0 37 1 0 CGCGAATGCCGGGCCACATGGCTTCCAAGACGT 807 CTGCCCCACCACACGCGTGGCGGATGT PKN1 NM_002741 9 19 + 70 + 0 37 1 0 CGCGAATGCCCAATGCCTGGGACCAGAGCTTCA 808 CTCTGGAGCTGGACGCGTGGCGGATGT PKN1 NM_002741 9 19 + 105 − 0 37 1 0 CGCGAATGCCTAGGCCCTGCTGTCCTCCAACGC 809 AGCTCACCCTTTACGCGTGGCGGATGT PKN1 NM_002741 9 19 + 140 + 0 37 1 0 CGCGAATGCCGAGGAGGAGGGGTTCCATGCCTC 810 TGGCACCCTGAGACGCGTGGCGGATGT PKN1 NM_002741 9 19 + 175 − 0 37 1 0 CGCGAATGCCTTCCCGTGCCTGGAGGGAGAGGA 811 AGAGGGCCATCAACGCGTGGCGGATGT PKN1 NM_002741 9 19 + 210 + 0 37 1 0 CGCGAATGCCCTGGAGTTGGCTGTGTTCTGGCG 812 GGACCAGCGGGGACGCGTGGCGGATGT PKN1 NM_002741 9 19 + 245 − 0 37 1 0 CGCGAATGCCAATCCTCCAACTTCAGGAATTTG 813 AGGGCACACAGGACGCGTGGCGGATGT PKN1 NM_002741 9 19 + 280 + 0 37 1 0 CGCGAATGCCTCTTGGACAATGAGAGGCATGAG 814 GTGCAGCTGGACACGCGTGGCGGATGT PKN1 NM_002741 9 19 + 315 − 0 37 1 0 CGCGAATGCCTATACCTCAGCCACCAGGCAGCC 815 CTGGGGTTCCATACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 0 + 0 37 1 0 CGCGAATGCCTGATGTCGTCATCGTGGAGCGTG 816 GGAAGGGCGACGACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 35 − 0 37 1 0 CGCGAATGCCCTTCATCCTGCCAAACTTCCTCCT 817 CTCGGGAACACACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 70 + 0 37 1 0 CGCGAATGCCCTCCTGCAGTTCTGTGAGAACCA 818 CCGGCCTGCCTAACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 105 − 0 37 1 0 CGCGAATGCCGGATGAGTGCCGTCTTCTTATTCC 819 AGGTACCCCAGACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 140 + 0 37 1 0 CGCGAATGCCGCGCGCGAGACCCCTGGGCCCAG 820 GACACGGTGAGCACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 175 − 0 37 1 0 CGCGAATGCCTGAGGAGGTACGGGGACGGAGG 821 CACTCTGGGGCTAACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 210 + 0 37 1 0 CGCGAATGCCCTGTGCCCCTTTCCTCCAGCCCCA 822 AAGACAGTTGTACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 245 − 0 37 1 0 CGCGAATGCCCCAGGAGCTTCTGAGAAAAGAAA 823 CACATGGGACTCACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 280 + 0 37 1 0 CGCGAATGCCACTATGAGGTGGACAGTGATGAG 824 GAGTGGGAAGAAACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 315 − 0 37 1 0 CGCGAATGCCCCCTCACTGTGGGACAGGGACTC 825 CCCAGGCTCCTCACGCGTGGCGGATGT CHAF1A NM_005483 10 19 + 350 + 0 37 1 0 CGCGAATGCCGGTAAGGATGTGCCCCAGCTGTC 826 TTCACTCACAGAACGCGTGGCGGATGT PDGFRA NM_006206 22 4 + 0 + 0 37 1 0 CGCGAATGCCAGTTATGAAAAAATTCACCTGGA 827 CTTCCTGAAGAGACGCGTGGCGGATGT PDGFRA NM_006206 22 4 + 35 − 0 37 1 0 CGCGAATGCCAGTCCACACGCATGCGTGCCACA 828 GCAGGATGGTCAACGCGTGGCGGATGT PDGFRA NM_006206 22 4 + 70 + 0 37 1 0 CGCGAATGCCCAGACAATGCATACATTGGTGTC 829 ACCTACAAAAACACGCGTGGCGGATGT PDGFRA NM_006206 22 4 + 105 − 0 37 1 0 CGCGAATGCCAGACCACCCTCCCAGTCCTTCAG 830 CTTGTCTTCCTCACGCGTGGCGGATGT PDGFRA NM_006206 22 4 + 140 + 0 37 1 0 CGCGAATGCCGGATGAGCAGAGACTGAGCGCTG 831 ACAGTGGCTACAACGCGTGGCGGATGT PDGFRA NM_006206 22 4 + 175 − 0 37 1 0 CGCGAATGCCCTCAGGGACAGGGTCAATGTCAG 832 GCAGAGGAATGAACGCGTGGCGGATGT PDGFRA NM_006206 22 4 + 210 + 0 37 1 0 CGCGAATGCCGAGGAGGACCTGGGCAAGAGGA 833 ACAGACACAGGTAACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 0 + 0 37 1 0 CGCGAATGCCCTTTTATTCCGCCTGCGTGGTGCT 834 GGGCCTACAGTACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 35 − 0 37 1 0 CGCGAATGCCCACACACCTGTAGACGATCTTGT 835 GTTCGTGAAGAAACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 70 + 0 37 1 0 CGCGAATGCCCGTGTGTGCATGCATGTGCACAC 836 TGCCCGTTGTGGACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 105 − 0 37 1 0 CGCGAATGCCGGAAAGAGGGATGAGCGGGGGT 837 CTGGGTCCTGTCCACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 140 + 0 37 1 0 CGCGAATGCCACCCCTCCAACCCCCAACAGGGA 838 CCTGAAGTTGGAACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 175 − 0 37 1 0 CGCGAATGCCTCTTGACGTAGCCCTCGGTGTCC 839 AGGAGCAAATTGACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 210 + 0 37 1 0 CGCGAATGCCTCGCAGACTTTGGCCTCTGCAAG 840 GAGGGTGAGGGGACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 245 − 0 37 1 0 CGCGAATGCCTTCCTTCCCCCTCCTTTGTCTAAT 841 CCAGAGGCCAGACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 280 + 0 37 1 0 CGCGAATGCCTGGGGTCCCTAACCCTATTCGGG 842 GTGCCCCCCACCACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 315 − 0 37 1 0 CGCGAATGCCGGTTGTCCACACAGCCAGCCCTG 843 TCCCAGGGTCAGACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 350 + 0 37 1 0 CGCGAATGCCCCACATTGGGCTGAGTGACTCCT 844 CTGGCCCCCATAACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 385 − 0 37 1 0 CGCGAATGCCAGCCCATCCCTGGGGACAGCAAG 845 GCCATGTGAGGTACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 420 + 0 37 1 0 CGCGAATGCCATGGGGACCGGACCAGCACATTC 846 TGTGGGACCCCGACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 455 − 0 37 1 0 CGCGAATGCCGACGTGTCCGTCAGCACCTCAGG 847 GGCCAGGAACTCACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 490 + 0 37 1 0 CGCGAATGCCGTACACGCGAGCTGTGGACTGGT 848 GGGGACTGGGTGACGCGTGGCGGATGT PKN1 NM_002741 19 19 + 525 − 0 37 1 0 CGCGAATGCCTCTCACCTCGCCAACCAGCATCT 849 CGTAGAGCAGCAACGCGTGGCGGATGT EPHA7 NM_004440 10 6 − −16 + 0 37 1 0 CGCGAATGCCTTTCCTCTGTTTACAGCTACAGCT 850 GTCTCCAGTGAACGCGTGGCGGATGT EPHA7 NM_004440 10 6 − 19 − 0 37 1 0 CGCGAATGCCCAGCAACCACAGCAATGATAATA 851 ACAGGATTCTGTACGCGTGGCGGATGT EPHA7 NM_004440 10 6 − 54 + 0 37 1 0 CGCGAATGCCTAGCTGGGACCATCATTTTGGTG 852 TTCATGGTCTTTACGCGTGGCGGATGT EPHA7 NM_004440 10 6 − 89 − 0 37 1 0 CGCGAATGCCAACTTTGTGTTCTACCTTCTCCCA 853 ATGATGAAGCCACGCGTGGCGGATGT PIK3CA NM_006218 11 3 + −29 + 0 37 1 0 CGCGAATGCCTTTATGTTTATTTTGTTTCTCCCAC 854 ACAGACACTAACGCGTGGCGGATGT PIK3CA NM_006218 11 3 + 6 − 0 37 1 0 CGCGAATGCCGCAATTTGGGTAGAATTTCGGGG 855 ATAGTTACACAAACGCGTGGCGGATGT PIK3CA NM_006218 11 3 + 39 + 0 37 1 0 CGCGAATGCCGCTTCTGTCTGTTAAATGGAATTC 856 TAGAGATGAAGACGCGTGGCGGATGT PIK3CA NM_006218 11 3 + 76 − 0 0 2 0 CGCGAATGCCTTATCTAGTAATCTCAAACATAC 857 ATTTACCTGGGCACGCGTGGCGGATGT NTRK3 NM_001012338 10 15 − −58 + 0 37 1 0 CGCGAATGCCTGTGTCTGTTCTGGTTTTTATTAA 858 ATTTGTTAATTACGCGTGGCGGATGT NTRK3 NM_001012338 10 15 − −23 − 0 37 1 0 CGCGAATGCCTATCCGTGCTCTCTGCAAAAAAA 859 GGACAAAGAGATACGCGTGGCGGATGT NTRK3 NM_001012338 10 15 − 12 + 0 37 1 0 CGCGAATGCCACTTTATCTTGTGTAAGTCTGCTT 860 TACCTGTTGCTACGCGTGGCGGATGT NTRK3 NM_001012338 10 15 − 47 − 0 37 1 0 CGCGAATGCCTAGTTACCGATAGTATCAGAATA 861 AATCAGTTTCAAACGCGTGGCGGATGT RBBP8 NM_002894 9 18 + −21 + 0 37 1 0 CGCGAATGCCTGGTTTATTATTTATTCTTAGAAG 862 CACATGGAACAACGCGTGGCGGATGT RBBP8 NM_002894 9 18 + 14 − 0 37 1 0 CGCGAATGCCAAATTAAAAGATGACTTATCAGG 863 GGTATAGCTGCTACGCGTGGCGGATGT RBBP8 NM_002894 9 18 + 49 + 0 37 1 0 CGCGAATGCCAGCTACAGTTGTTGCTGAAACAC 864 TTGGACTTGGTGACGCGTGGCGGATGT RBBP8 NM_002894 9 18 + 84 − 0 37 1 0 CGCGAATGCCCCAAACTAAACAATTACTTACAG 865 ATTCTTCTTGAAACGCGTGGCGGATGT CHAF1A NM_005483 8 19 + 0 + 0 37 1 0 CGCGAATGCCACCCTGGCCGGCTCCTGTGGGAA 866 GTTTGCCCCCTTACGCGTGGCGGATGT CHAF1A NM_005483 8 19 + 35 − 0 37 1 0 CGCGAATGCCGCCGAGGGGCCAGGACCATGTGC 867 TCTTTAATTTCAACGCGTGGCGGATGT CHAF1A NM_005483 8 19 + 70 + 0 37 1 0 CGCGAATGCCGTCGGACCGCTTTCCATCCAGAC 868 CTCTGCAGTCAGACGCGTGGCGGATGT CHAF1A NM_005483 8 19 + 105 − 0 37 1 0 CGCGAATGCCAACTCGCCGCTCTGCTGCTGGAG 869 GAGCTGGTCCAGACGCGTGGCGGATGT CHAF1A NM_005483 8 19 + 140 + 0 37 1 0 CGCGAATGCCCTCCTTCTTGAAAGACCTCAAAG 870 GCCGGCAGCCCCACGCGTGGCGGATGT CHAF1A NM_005483 8 19 + 175 − 0 37 1 0 CGCGAATGCCATTCCGGGTGGAAACGTGCGTGG 871 GTCCGGACCTCAACGCGTGGCGGATGT CHAF1A NM_005483 8 19 + 210 + 0 37 1 0 CGCGAATGCCGCAGATATTTTTAACAGGTCAGA 872 GCCTGAGGAGGTACGCGTGGCGGATGT RPS6KA1 NM_002953 17 1 + 0 + 0 37 1 0 CGCGAATGCCGTGTATGATGATGGCAAACACGT 873 GTACCTGGTGACACGCGTGGCGGATGT RPS6KA1 NM_002953 17 1 + 35 − 0 37 1 0 CGCGAATGCCTCTTGTCCAGCAGCTCCCCACCCC 874 GCATCAGCTCTACGCGTGGCGGATGT RPS6KA1 NM_002953 17 1 + 70 + 0 37 1 0 CGCGAATGCCTCCTGCGGCAGAAGTTCTTCTCA 875 GAGCGGGAGGCCACGCGTGGCGGATGT RPS6KA1 NM_002953 17 1 + 105 − 0 37 1 0 CGCGAATGCCTCCACAGTTTTGCCAATGGTGTG 876 CAGGACAAAGCTACGCGTGGCGGATGT RPS6KA1 NM_002953 17 1 + 140 + 0 37 1 0 CGCGAATGCCGTATCTGCACTCACAGGGGGTGA 877 GTCTGGATTCGGACGCGTGGCGGATGT EPHA7 NM_004440 6 6 − −39 + 0 37 1 0 CGCGAATGCCCAGGTTACGACTCCTGAAACTTT 878 TTTTTTTTAATTACGCGTGGCGGATGT EPHA7 NM_004440 6 6 − −4 − 0 37 1 0 CGCGAATGCCATGAACTCTATTACTATCATGACT 879 GGTTTCCCTAAACGCGTGGCGGATGT EPHA7 NM_004440 6 6 − 31 + 0 37 1 0 CGCGAATGCCGGAAAATGGAGCCCTAGATGCAT 880 TTCTCAGGGTAAACGCGTGGCGGATGT EPHA7 NM_004440 6 6 − 66 − 0 37 1 0 CGCGAATGCCATAGTATATTTAGAATAAGTGGA 881 TCACTTTGGTACACGCGTGGCGGATGT RB1 NM_000321 21 13 + −18 + 0 37 1 0 CGCGAATGCCCATCAATTTATTTACTAGATTATG 882 ATGTGTTCCATACGCGTGGCGGATGT RB1 NM_000321 21 13 + 17 − 0 37 1 0 CGCGAATGCCTAAGGTCTATATTCTTCACTTTGC 883 ATATGCCATACACGCGTGGCGGATGT RB1 NM_000321 21 13 + 52 + 0 37 1 0 CGCGAATGCCAATTCAAAATCATTGTAACAGCA 884 TACAAGGATCTTACGCGTGGCGGATGT RB1 NM_000321 21 13 + 87 − 0 37 1 0 CGCGAATGCCTATGGAAAATTACCTACCTCCTG 885 AACAGCATGAGGACGCGTGGCGGATGT KSR2 NM_173598 11 12 − 0 + 0 37 1 0 CGCGAATGCCGACCACATCCCTGTCCCTTACCA 886 GCCAGACTCCAGACGCGTGGCGGATGT KSR2 NM_173598 11 12 − 35 − 0 37 1 0 CGCGAATGCCAGGGCGTGGAGGACGTCGTGGA 887 GGAGGGGTTGCTGACGCGTGGCGGATGT KSR2 NM_173598 11 12 − 70 + 0 37 1 0 CGCGAATGCCCCTCGCCAGCACCCCCCCTCCCTC 888 CTAGTGCCACGACGCGTGGCGGATGT KSR2 NM_173598 11 12 − 105 − 0 37 1 0 CGCGAATGCCGTGCACTGTGGGGAAGGGTGTAG 889 GGGAGAAGGCGGACGCGTGGCGGATGT KSR2 NM_173598 11 12 − 140 + 0 37 1 0 CGCGAATGCCACGGCAGCAGAAGAACTTCAACC 890 TGCCAGGTACCTACGCGTGGCGGATGT RPS6KA1 NM_002953 12 1 + −38 + 0 37 1 0 CGCGAATGCCGCCAGCCAGGGACAGACCCTTCA 891 TTTGGGCTCTTTACGCGTGGCGGATGT RPS6KA1 NM_002953 12 1 + −3 − 0 37 1 0 CGCGAATGCCCTTGATTTCCTCTGCCCCATCAGG 892 GCCGGAGCCTGACGCGTGGCGGATGT RPS6KA1 NM_002953 12 1 + 32 + 0 37 1 0 CGCGAATGCCCGGCATGTCTTCTACTCCACCATT 893 GACTGGAATGTACGCGTGGCGGATGT RPS6KA1 NM_002953 12 1 + 67 − 0 37 1 0 CGCGAATGCCCCCTGGCCAGAGCCCTGGTGTGG 894 GTGGACACACTCACGCGTGGCGGATGT PALB2 NM_024675 2 16 − 0 + 0 37 1 0 CGCGAATGCCGGGCTTCTCTTTATTGTCCTGAGT 895 CATCCCTGTGCACGCGTGGCGGATGT PALB2 NM_024675 2 16 − 35 − 0 37 1 0 CGCGAATGCCGAAACACAGGGCTTCGCAACGAC 896 TCACTCTCTTTGACGCGTGGCGGATGT PALB2 NM_024675 2 16 − 70 + 0 37 1 0 CGCGAATGCCAGCTCATTGTGATTAACCCTAAG 897 ACGACTCTCAGCACGCGTGGCGGATGT PALB2 NM_024675 2 16 − 105 − 0 37 1 0 CGCGAATGCCTGCCCTGGAGGAAGACAGTACAG 898 CATCACACCCACACGCGTGGCGGATGT PALB2 NM_024675 2 16 − 140 + 0 37 1 0 CGCGAATGCCGGCTGGCAGGCAAGTGTGCATAA 899 CTGCTACTCTATACGCGTGGCGGATGT EPHA3 NM_005233 16 3 + 0 + 0 37 1 0 CGCGAATGCCGCCATCAAACCTTCTTCTGGACC 900 AAAGCAATGTGGACGCGTGGCGGATGT EPHA3 NM_005233 16 3 + 35 − 0 37 1 0 CGCGAATGCCAAGCCAGTCACCTGTTGTGCGGA 901 AGGTAGTGATATACGCGTGGCGGATGT EPHA3 NM_005233 16 3 + 70 + 0 37 1 0 CGCGAATGCCAATGGTGTCTGGACAGCACACTG 902 CAAGGAAATCTTACGCGTGGCGGATGT EPHA3 NM_005233 16 3 + 105 − 0 37 1 0 CGCGAATGCCCTATTGTGTCACAAGAACTGTAC 903 TCCACACCCGTGACGCGTGGCGGATGT EPHA3 NM_005233 16 3 + 140 + 0 37 1 0 CGCGAATGCCCCAAGATTTCCACAGAGTAAGAA 904 AAAAAAATTCATACGCGTGGCGGATGT RET NM_020630 12 10 + 0 + 0 37 1 0 CGCGAATGCCGAGGATCCAAAGTGGGAATTCCC 905 TCGGAAGAACTTACGCGTGGCGGATGT RET NM_020630 12 10 + 35 − 0 37 1 0 CGCGAATGCCCAAATTCGCCTTCTCCTAGAGTTT 906 TTCCAAGAACCACGCGTGGCGGATGT RET NM_020630 12 10 + 70 + 0 37 1 0 CGCGAATGCCGAAAAGTGGTCAAGGCAACGGC 907 CTTCCATCTGAAAACGCGTGGCGGATGT RET NM_020630 12 10 + 105 − 0 37 1 0 CGCGAATGCCATCTTCACGGCCACCGTGGTGTA 908 CCCTGCTCTGCCACGCGTGGCGGATGT RET NM_020630 12 10 + 140 + 0 37 1 0 CGCGAATGCCGCTGAAAGGTACCTGCCAGGCAC 909 AGGCACAGTGCCACGCGTGGCGGATGT RB1 NM_000321 27 13 + −33 + 0 37 1 0 CGCGAATGCCAATGCTGTTAACAGTTCTTCATCC 910 TTTTTCCAGCTACGCGTGGCGGATGT RB1 NM_000321 27 13 + 2 − 0 37 1 0 CGCGAATGCCTTCATTTTCTGCTTTTGCATTCGT 911 GTTCGAGTAGAACGCGTGGCGGATGT RB1 NM_000321 27 13 + 37 + 0 37 1 0 CGCGAATGCCTGATAGCATGGATACCTCAAACA 912 AGGAAGAGAAATACGCGTGGCGGATGT RB1 NM_000321 27 13 + 72 − 0 37 1 0 CGCGAATGCCGTGTACACAGTGTCCACCAAGGT 913 CCTGAGATCCTCACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 0 + 0 37 1 0 CGCGAATGCCAGCTCCCCCATCCAGGAATCCAC 914 TGCTCCCGAGCTACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 35 − 0 37 1 0 CGCGAATGCCGGGCGGGGCCTGGGGTCTCCTGG 915 GTCTCCGAAGGCACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 70 + 0 37 1 0 CGCGAATGCCTGTGCAGGTGACACCACTCCCTG 916 GCCCCCTGTCCAACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 105 − 0 37 1 0 CGCGAATGCCCCACTGGTGAGAACAAGGACAGC 917 GGGCAGGGGTGGACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 140 + 0 37 1 0 CGCGAATGCCCCTCTCTCCACAGCCCTCTGAGG 918 AAGTCACCTCTGACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 175 − 0 37 1 0 CGCGAATGCCCCCAGCACCGCCAGGAACTTGAA 919 ATCTTCGAGGGTACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 210 + 0 37 1 0 CGCGAATGCCCCGGGGTCATTTTGGGAAGGTGA 920 GGTGGAGGGCAGACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 245 − 0 37 1 0 CGCGAATGCCCGGCCAGCCAGGGACCTGGGGG 921 GTCTCCCAATTCCACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 280 + 0 37 1 0 CGCGAATGCCCTAAAACCACCCCTGCCCACTGT 922 GGTTCCAGGTGCACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 315 − 0 37 1 0 CGCGAATGCCGAACAGCTCCCCACTGGGCCGGA 923 ATTCGGAGAGGAACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 350 + 0 37 1 0 CGCGAATGCCGCCATCAAGGCTCTGAAGAAAGG 924 GGACATTGTGGCACGCGTGGCGGATGT PKN1 NM_002741 15 19 + 385 − 0 37 1 0 CGCGAATGCCCCACGGCAGGTCCCCACCTCTCC 925 ACCTCGTCTCGGACGCGTGGCGGATGT CHAF1A NM_005483 1 19 + −44 + 0 37 1 0 CGCGAATGCCCGCGCCTCCGCCGCCTGAGAGGA 926 GGTCGAGCTGCCACGCGTGGCGGATGT CHAF1A NM_005483 1 19 + −9 − 0 37 1 0 CGCGAATGCCGCCCCGCACTCCAGCTCCTCCAG 927 CATCGCCCCGGCACGCGTGGCGGATGT CHAF1A NM_005483 1 19 + 26 + 0 37 1 0 CGCGAATGCCGCCCGGCGCCAGGGGAGCCGCCA 928 CAGGTCGGTTCGACGCGTGGCGGATGT CHAF1A NM_005483 1 19 + 57 − 13 37 1 0 CGCGAATGCCGCCGCGCCCCCCCTTCCCCTCGG 929 CGCGGGCCCGAAACGCGTGGCGGATGT GUCY2F NM_001522 11 X − 0 + 0 37 1 0 CGCGAATGCCGGCATGAAGTACTTACACCACAG 930 AGAGTTTGTTCAACGCGTGGCGGATGT GUCY2F NM_001522 11 X − 35 − 0 37 1 0 CGCGAATGCCCATCTACCACACAGTTTCGAGAC 931 TTTAGCCTCCCAACGCGTGGCGGATGT GUCY2F NM_001522 11 X − 70 + 0 37 1 0 CGCGAATGCCGGCGTTTTGTACTAAAAGTGACA 932 GATTATGGCTTTACGCGTGGCGGATGT GUCY2F NM_001522 11 X − 105 − 0 37 1 0 CGCGAATGCCTCTTCAGAGAGTCTCAGCATTTCT 933 AAGATGTCGTTACGCGTGGCGGATGT GUCY2F NM_001522 11 X − 140 + 0 37 1 0 CGCGAATGCCGGAATCTTCTATGGAAGGTAAGC 934 AATGAATTGTACACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 0 + 0 37 1 0 CGCGAATGCCGATTACATCCACAGTACCCACGG 935 CAAGGAGATGGAACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 35 − 0 37 1 0 CGCGAATGCCGCTTGCCCGGGACTTTGACTGTT 936 GTTCGCAGCAAGACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 70 + 0 37 1 0 CGCGAATGCCGGCCCCCGAGGGCCATCTCTGCC 937 TTTGGCCCCTCAACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 105 − 0 37 1 0 CGCGAATGCCGTGCTCATGTCCTTGACGAGCCC 938 GTTAATGCTGGCACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 140 + 0 37 1 0 CGCGAATGCCTGTCCAGATGGGTGAAGGCCTGG 939 GTGAGTAAGGTTACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 175 − 0 37 1 0 CGCGAATGCCCCCAGCCCTGGCCCCTCTTCTGCT 940 CCTATGTCATAACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 210 + 0 37 1 0 CGCGAATGCCGAAAAAGAGGGGTAAGAGGGAA 941 GAGGGGGCTGGCAACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 245 − 0 37 1 0 CGCGAATGCCGTCCCTCTTTATCACCCAGCTTCC 942 TACTCGCCCAGACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 280 + 0 37 1 0 CGCGAATGCCCACATAAGGAATGGGGCCAGAA 943 GAAAGAGGCTCACACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 315 − 0 37 1 0 CGCGAATGCCAGTAGTGGCTTCTGTCGGAAGAA 944 GATGAAACCTCAACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 350 + 0 37 1 0 CGCGAATGCCCCCATGCCAAGCCCTAGCCCCAG 945 CCCCAGTTCCCTACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 385 − 0 37 1 0 CGCGAATGCCGCAGGTGTTTGGATGTCTGATCT 946 GGTGGTGGCTGCACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 420 + 0 37 1 0 CGCGAATGCCTGAAGCCAGACCGGAATTTGGCC 947 CGAGCCCTCAGCACGCGTGGCGGATGT CENTG1 NM_014770 8 12 − 455 − 0 37 1 0 CGCGAATGCCAGACAGCAGATGGAAGGTCATCC 948 CCACTGACCCGTACGCGTGGCGGATGT EPHA7 NM_004440 8 6 − −7 + 0 37 1 0 CGCGAATGCCATTACAGTTAAATTTCCAGGCAC 949 CAAAACCTACATACGCGTGGCGGATGT EPHA7 NM_004440 8 6 − 28 − 0 37 1 0 CGCGAATGCCCAGCTCTATTTGGGTCCTCATAG 950 GTTTCAGGGTCAACGCGTGGCGGATGT EPHA7 NM_004440 8 6 − 63 + 0 37 1 0 CGCGAATGCCTCCATCAATTCGCCAAGGAGCTA 951 GATGCCTCCTGTACGCGTGGCGGATGT EPHA7 NM_004440 8 6 − 98 − 0 37 1 0 CGCGAATGCCGCCTTACCTGCACCAATCACACG 952 CTCAATTTTAATACGCGTGGCGGATGT EPHB1 NM_004441 10 3 + −8 + 0 37 1 0 CGCGAATGCCCCAACAAGGCTCCCCAGGGATGA 953 AGATCTACATTGACGCGTGGCGGATGT EPHB1 NM_004441 10 3 + 27 − 0 37 1 0 CGCGAATGCCGACAGCTTCGTTGGGATCCTCGT 954 AAGTGAAGGGGTACGCGTGGCGGATGT EPHB1 NM_004441 10 3 + 62 + 0 37 1 0 CGCGAATGCCCGGGAGTTTGCCAAGGAGATTGA 955 TGTATCTTTTGTACGCGTGGCGGATGT EPHB1 NM_004441 10 3 + 97 − 0 37 1 0 CGCGAATGCCGAGCCATACCTGCTCCGATGACC 956 TCTTCAATTTTCACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 0 + 0 37 1 0 CGCGAATGCCGAACACTGTCCATTGGCATGGGG 957 AAATATAAACTTACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 35 − 0 37 1 0 CGCGAATGCCTTTTTCCAGATACTAGAGTGTCTG 958 TGTAATCAAACACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 70 + 0 37 1 0 CGCGAATGCCTGGCTTTGAATCTTTGGCCAGTAC 959 CTCATGGATTAACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 105 − 0 37 1 0 CGCGAATGCCGATCCAGTAACACCAATAGGGTT 960 CAGCAAATCTTCACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 140 + 0 37 1 0 CGCGAATGCCAAATCCAAATAAAGTAAGGTTTT 961 TATTGTCATAAAACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 175 − 0 37 1 0 CGCGAATGCCAGAGAGAAGGTTTGACTGCCATA 962 AAAAATATCTAAACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 210 + 0 37 1 0 CGCGAATGCCTATGTATATATAATAGCTTTTCTT 963 CCATCTCTTAGACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 245 − 0 37 1 0 CGCGAATGCCAACCAGTCAAACTCCAACTCTAA 964 GCATGGAGTTTCACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 280 + 0 37 1 0 CGCGAATGCCCAGCAGTGTGGTAAAGTTCCCAG 965 ATATGTCAGTGAACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 315 − 0 37 1 0 CGCGAATGCCTTCTCGGGATACAGACCAATTGG 966 CATGCTCTTCAAACGCGTGGCGGATGT PIK3CA NM_006218 9 3 + 350 + 0 37 1 0 CGCGAATGCCGCAGGATTTAGCTATTCCCACGC 967 AGGACTGGTAAGACGCGTGGCGGATGT RET NM_020630 16 10 + −34 + 0 37 1 0 CGCGAATGCCTAACTTCAATGTCTTTATTCCATC 968 TTCTCTTTAGGACGCGTGGCGGATGT RET NM_020630 16 10 + 1 − 0 37 1 0 CGCGAATGCCGGATTCAATTGCCATCCATTTAA 969 CTGGAATCCGACACGCGTGGCGGATGT RET NM_020630 16 10 + 36 + 0 37 1 0 CGCGAATGCCCTTTTTGATCATATCTACACCACG 970 CAAAGTGATGTACGCGTGGCGGATGT RET NM_020630 16 10 + 71 − 0 37 1 0 CGCGAATGCCAACCTCCACCCCAAGAGAGCAAC 971 ACCCACACTTACACGCGTGGCGGATGT PIK3CA NM_006218 17 3 + −30 + 0 37 1 0 CGCGAATGCCAAATGGTGATACATATTATTTGA 972 ATTTCAGATTTAACGCGTGGCGGATGT PIK3CA NM_006218 17 3 + 5 − 0 37 1 0 CGCGAATGCCATACGAATAATTTGAAGTGTTAG 973 CATATCTTGCCGACGCGTGGCGGATGT PIK3CA NM_006218 17 3 + 40 + 0 37 1 0 CGCGAATGCCTATGGAAAATATCTGGCAAAATC 974 AAGGTCTTGATCACGCGTGGCGGATGT PIK3CA NM_006218 17 3 + 75 − 0 37 1 0 CGCGAATGCCCAACATACAGGTTGCCTTACTGG 975 TTACCTACCGAAACGCGTGGCGGATGT RB1 NM_000321 4 13 + −10 + 0 37 1 0 CGCGAATGCCTCCTTTGTAGTGTCCATAAATTCT 976 TTAACTTACTAACGCGTGGCGGATGT RB1 NM_000321 4 13 + 25 − 0 37 1 0 CGCGAATGCCGCATTATCAACTTTGGTACTGGT 977 ATCAATTTCTTTACGCGTGGCGGATGT RB1 NM_000321 4 13 + 60 + 0 37 1 0 CGCGAATGCCTATGTCAAGACTGTTGAAGAAGT 978 ATGATGTATTGTACGCGTGGCGGATGT RB1 NM_000321 4 13 + 95 − 0 37 1 0 CGCGAATGCCTTTACTTTACCTTTCCAATTTGCT 979 GAAGAGTGCAAACGCGTGGCGGATGT RPS6KA1 NM_002953 21 1 + −1 + 0 37 1 0 CGCGAATGCCGGACCTGGTGTCCAAGATGCTAC 980 ACGTGGATCCCCACGCGTGGCGGATGT RPS6KA1 NM_002953 21 1 + 34 − 0 37 1 0 CGCGAATGCCATGCTGCAGAACCTGCTTAGCTG 981 TGAGGCGCTGGTACGCGTGGCGGATGT RPS6KA1 NM_002953 21 1 + 69 + 0 37 1 0 CGCGAATGCCCCATGGGTCACCCAGAAAGACAA 982 GCTTCCCCAAAGACGCGTGGCGGATGT RPS6KA1 NM_002953 21 1 + 104 − 0 37 1 0 CGCGAATGCCCCTTCACAAGCTGTAGGTCCTGG 983 TGGGACAGCTGGACGCGTGGCGGATGT NFKB1 NM_003998 3 4 + −30 + 0 37 1 0 CGCGAATGCCATTGAAACATTTAAATGTTCTTCT 984 TTACAGATGTTACGCGTGGCGGATGT NFKB1 NM_003998 3 4 + 5 − 0 37 1 0 CGCGAATGCCTAAATATTGTATGAGTCAAAGAA 985 GGATCCAAATGAACGCGTGGCGGATGT NFKB1 NM_003998 3 4 + 40 + 0 37 1 0 CGCGAATGCCATCCAGAAGTATTTCAACCACAG 986 ATGGCACTGCCAACGCGTGGCGGATGT NFKB1 NM_003998 3 4 + 75 − 0 37 1 0 CGCGAATGCCACAACAGGGTAACAGGGATGAG 987 TTTTCTTACCTGTACGCGTGGCGGATGT EPHA3 NM_005233 17 3 + −17 + 0 37 1 0 CGCGAATGCCGTTTTCTTTTTTTACAGTGACATG 988 AAAAAGGTTGGACGCGTGGCGGATGT EPHA3 NM_005233 17 3 + 18 − 0 37 1 0 CGCGAATGCCTGATGATCTTCTTCTGTGGCCCAA 989 CCACGGTGACAACGCGTGGCGGATGT EPHA3 NM_005233 17 3 + 53 + 0 37 1 0 CGCGAATGCCGTAGCATTAAAGCTCTAGAAACG 990 CAATCAAAGAATACGCGTGGCGGATGT EPHA3 NM_005233 17 3 + 88 − 0 37 1 0 CGCGAATGCCCACTTCCGTCCCGTGCTTTACACG 991 GGAACTGGGCCACGCGTGGCGGATGT NTRK3 NM_001007156 2 15 − −6 + 19 37 1 0 CGCGAATGCCTGCCAGGGGTCTTTTCAAACATA 992 GACAATCATGGGACGCGTGGCGGATGT NTRK3 NM_001007156 2 15 − 33 − 35 37 1 0 CGCGAATGCCTGGGACTAGATGATCTCTATTGT 993 CCTTCAAGTTTAACGCGTGGCGGATGT NTRK3 NM_001007156 2 15 − 68 + 35 37 1 0 CGCGAATGCCTCAACTCACTATATATATGAGGA 994 ACCTGAGGTCCAACGCGTGGCGGATGT NTRK3 NM_001007156 2 15 − 103 − 35 37 1 0 CGCGAATGCCCACCATGTGACCTTGGGTAAGAC 995 ACTTCCCCACTCACGCGTGGCGGATGT EPHA4 NM_004438 3 2 − 0 + 0 37 1 0 CGCGAATGCCACCTAACACTGCCTTGTTGGATC 996 CAAGCTCCCCTGACGCGTGGCGGATGT EPHA4 NM_004438 3 2 − 35 − 0 37 1 0 CGCGAATGCCGAGCCAATCGCCCACTGATACCA 997 CAGCAGAGAATTACGCGTGGCGGATGT EPHA4 NM_004438 3 2 − 70 + 0 37 1 0 CGCGAATGCCCAGGCCATTAAAATGGACCGGTA 998 TAAGGATAACTTACGCGTGGCGGATGT EPHA4 NM_004438 3 2 − 105 − 0 37 1 0 CGCGAATGCCCCACAGCCTCTAGTGTGGTATAA 999 CCAGCAGCTGTGACGCGTGGCGGATGT EPHA4 NM_004438 3 2 − 140 + 0 37 1 0 CGCGAATGCCTGCACGTGAACCAGGAGTAAGTA 1000 CTCAACGATGTAACGCGTGGCGGATGT EPHA3 NM_005233 9 3 + −38 + 0 37 1 0 CGCGAATGCCTTCCTCTTATGTGTTCGCTTTCCTT 1001 GATTTACCTCACGCGTGGCGGATGT EPHA3 NM_005233 9 3 + −3 − 0 37 1 0 CGCGAATGCCCTGCCCCATGTTTTGACTTATAGC 1002 CACAGAACCTGACGCGTGGCGGATGT EPHA3 NM_005233 9 3 + 32 + 0 37 1 0 CGCGAATGCCATGAAAAAAGACTTCATTTTGGC 1003 AATGGGCATTGTACGCGTGGCGGATGT EPHA3 NM_005233 9 3 + 67 − 0 37 1 0 CGCGAATGCCGGTGAAGCAAAACAAAAAGCCA 1004 AGTTTAGAAACTTACGCGTGGCGGATGT EPHB1 NM_004441 7 3 + 0 + 0 37 1 0 CGCGAATGCCGAACACAATGAGTTCAACTCCTC 1005 CATGGCCAGGAGACGCGTGGCGGATGT EPHB1 NM_004441 7 3 + 35 − 0 37 1 0 CGCGAATGCCGCCGCAGCCCATCAATCCTTGCT 1006 GTGTTGGTCTGAACGCGTGGCGGATGT EPHB1 NM_004441 7 3 + 70 + 0 37 1 0 CGCGAATGCCCTGGCATGGTATATGTGGTACAG 1007 GTGCGTGCCCGCACGCGTGGCGGATGT EPHB1 NM_004441 7 3 + 105 − 0 37 1 0 CGCGAATGCCATCTTGCCACTGAACTTGCCGTA 1008 GCCAGCAACAGTACGCGTGGCGGATGT EPHB1 NM_004441 7 3 + 140 + 0 37 1 0 CGCGAATGCCGTGCTTCCAGACTCTGACTGACG 1009 GTAAGGGTCGGGACGCGTGGCGGATGT RB1 NM_000321 19 13 + 0 + 0 37 1 0 CGCGAATGCCGTATCTTTCTCCTGTAAGATCTCC 1010 AAAGAAAAAAGACGCGTGGCGGATGT RB1 NM_000321 19 13 + 35 − 0 37 1 0 CGCGAATGCCTGCATTTGCAGTAGAATTTACAC 1011 GCGTAGTTGAACACGCGTGGCGGATGT RB1 NM_000321 19 13 + 70 + 0 37 1 0 CGCGAATGCCGAGACACAAGCAACCTCAGCCTT 1012 CCAGACCCAGAAACGCGTGGCGGATGT RB1 NM_000321 19 13 + 105 − 0 37 1 0 CGCGAATGCCTATAAAACAGTGAAAGAGAGGT 1013 AGATTTCAATGGCACGCGTGGCGGATGT RB1 NM_000321 19 13 + 136 + 7 37 1 0 CGCGAATGCCTATAAAAAAGGTTAGTAGATGAT 1014 TATTTTCAAGAGACGCGTGGCGGATGT RBBP8 NM_002894 18 18 + 0 + 0 37 1 0 CGCGAATGCCTATTATGCAGATATGCCAGCAGA 1015 AGAAAGAGAAAAACGCGTGGCGGATGT RBBP8 NM_002894 18 18 + 35 − 0 37 1 0 CGCGAATGCCAGCGGAATCGGTGTCTTGAGCAG 1016 GAAGCCAATTTCACGCGTGGCGGATGT RBBP8 NM_002894 18 18 + 70 + 0 37 1 0 CGCGAATGCCACATTCCACCCAACACACCAGAG 1017 AATTTTTGGGAAACGCGTGGCGGATGT RBBP8 NM_002894 18 18 + 105 − 0 37 1 0 CGCGAATGCCCTTTCCATACAAGTCTGAGTGGA 1018 AGGAAAACCAACACGCGTGGCGGATGT RBBP8 NM_002894 18 18 + 136 + 9 37 1 0 CGCGAATGCCAAAGAGGTGAGAGTATAGATTGT 1019 AACATTTTATAAACGCGTGGCGGATGT KSR2 NM_173598 8 12 − −46 + 0 37 1 0 CGCGAATGCCCCTGCCTTTTCACCTAGGGATCAC 1020 GTTTATTTTTCACGCGTGGCGGATGT KSR2 NM_173598 8 12 − −11 − 0 37 1 0 CGCGAATGCCTGAAGTAATGGATTTCCTTCCAA 1021 GCTGTAAAGAAAACGCGTGGCGGATGT KSR2 NM_173598 8 12 − 24 + 0 37 1 0 CGCGAATGCCAATTGAAGTGGAGCCAACGTCGG 1022 AGGTGAGAATCAACGCGTGGCGGATGT KSR2 NM_173598 8 12 − 59 − 0 37 1 0 CGCGAATGCCGGGCAGTCCCTGGAAAATGTCGC 1023 TTCACTGCTCTCACGCGTGGCGGATGT RBBP8 NM_002894 10 18 + −10 + 0 37 1 0 CGCGAATGCCACTCTTGAAGGAAACTCAAGGTC 1024 CCATGAGCCCCCACGCGTGGCGGATGT RBBP8 NM_002894 10 18 + 21 − 0 37 1 0 CGCGAATGCCCCTTCCAGACAGTGGTAGAGCTC 1025 ATCACCAAGGGGACGCGTGGCGGATGT RBBP8 NM_002894 10 18 + 56 + 0 37 1 0 CGCGAATGCCAAATCACAAGAAACAGCCTTTTG 1026 AGGAATCTACAAACGCGTGGCGGATGT RBBP8 NM_002894 10 18 + 91 − 0 37 1 0 CGCGAATGCCTGCCCTTAATTACCTTAAACTATC 1027 TTCAGTATTTCACGCGTGGCGGATGT RB1 NM_000321 9 13 + −31 + 0 37 1 0 CGCGAATGCCAATGATCATGTTGTAACTTCATCT 1028 TTTTCAGGTGAACGCGTGGCGGATGT RB1 NM_000321 9 13 + 4 − 0 37 1 0 CGCGAATGCCCATAAAAGGTATAAAATTTTTGA 1029 AATAAACATTTTACGCGTGGCGGATGT RB1 NM_000321 9 13 + 39 + 0 37 1 0 CGCGAATGCCAATTCTCTTGGACTTGTAACATCT 1030 AATGGACTTCCACGCGTGGCGGATGT RB1 NM_000321 9 13 + 74 − 0 37 1 0 CGCGAATGCCTAATATTTTATTAAATTTCCTTTC 1031 AGATTACCTCTACGCGTGGCGGATGT RET NM_020630 5 10 + 0 + 0 37 1 0 CGCGAATGCCGACACCGTGGTGGCCACGCTGCG 1032 TGTCTTCGATGCACGCGTGGCGGATGT RET NM_020630 5 10 + 35 − 0 37 1 0 CGCGAATGCCGCCTCACCAGCTCCCCTGATGCA 1033 GGTACCACGTCTACGCGTGGCGGATGT RET NM_020630 5 10 + 70 + 0 37 1 0 CGCGAATGCCGGTACACAAGCACGCTGCTCCCC 1034 GGGGACACCTGGACGCGTGGCGGATGT RET NM_020630 5 10 + 105 − 0 37 1 0 CGCGAATGCCTTGGGCCAGTGTTCCACCCGGAA 1035 GGTCTGCTGGGCACGCGTGGCGGATGT RET NM_020630 5 10 + 140 + 0 37 1 0 CGCGAATGCCCGAGACCTCGGTCCAGGCCAACG 1036 GCAGCTTCGTGCACGCGTGGCGGATGT RET NM_020630 5 10 + 175 − 0 37 1 0 CGCGAATGCCCCAGCCCCTCTTACTATAGTCATG 1037 TACGGTCGCCCACGCGTGGCGGATGT PKN1 NM_002741 22 19 + 0 + 0 37 1 0 CGCGAATGCCACTCTGGGCTGGGAAGCCCTGTT 1038 GGCCCGGCGCCTACGCGTGGCGGATGT PKN1 NM_002741 22 19 + 35 − 0 37 1 0 CGCGAATGCCTGCGGCCGGACAGCGTGGGCACA 1039 AAGGGCGGTGGCACGCGTGGCGGATGT PKN1 NM_002741 22 19 + 70 + 0 37 1 0 CGCGAATGCCCCGACGTCAGCAACTTCGACGAG 1040 GAGTTCACCGGGACGCGTGGCGGATGT PKN1 NM_002741 22 19 + 105 − 0 37 1 0 CGCGAATGCCCGCGCGTCGCGGGGCGGGCTCAG 1041 TGTGGGGGCCTCACGCGTGGCGGATGT PKN1 NM_002741 22 19 + 140 + 0 37 1 0 CGCGAATGCCGCCCCTCACAGCCGCGGAGCAGG 1042 CAGCCTTCCTGGACGCGTGGCGGATGT PKN1 NM_002741 22 19 + 171 − 3 37 1 0 CGCGAATGCCGGCTAGCAGCCCCCGGCCACGAA 1043 GTCGAAGTCCAGACGCGTGGCGGATGT PIK3CA NM_006218 13 3 + −18 + 0 23 1 1 CGCGAATGCCTTTTTTGGAATCACCTAGGTCCTA 1044 AAATATGAACAACGCGTGGCGGATGT PIK3CA NM_006218 13 3 + 17 − 0 0 2 0 CGCGAATGCCTCAGTAAAAATCTCACAAGCAAG 1045 TTATCCAAATATACGCGTGGCGGATGT PIK3CA NM_006218 13 3 + 52 + 0 0 2 0 CGCGAATGCCAGAAAGCATTGACTAATCAAAGG 1046 ATTGGGCACTTTACGCGTGGCGGATGT PIK3CA NM_006218 13 3 + 87 − 0 0 2 0 CGCGAATGCCGAAAATAATTAGACTTACTTTAA 1047 ATGCCAAAAGAAACGCGTGGCGGATGT PDGFRA NM_006206 3 4 + 0 + 0 37 1 0 CGCGAATGCCGGCTGAGCCTAATCCTCTGCCAG 1048 CTTTCATTACCCACGCGTGGCGGATGT PDGFRA NM_006206 3 4 + 35 − 0 37 1 0 CGCGAATGCCTGCACAACCTTTTCATTTTCATTT 1049 GGAAGGATAGAACGCGTGGCGGATGT PDGFRA NM_006206 3 4 + 70 + 0 37 1 0 CGCGAATGCCGCTGAATTCATCCTTTTCTCTGAG 1050 ATGCTTTGGGGACGCGTGGCGGATGT PDGFRA NM_006206 3 4 + 105 − 0 37 1 0 CGCGAATGCCTTCAGACATGGGGTACTGCCAGC 1051 TCACTTCACTCTACGCGTGGCGGATGT PDGFRA NM_006206 3 4 + 140 + 0 37 1 0 CGCGAATGCCGAAGAGAGCTCCGATGTGGAAAT 1052 CAGAAATGAAGAACGCGTGGCGGATGT PDGFRA NM_006206 3 4 + 175 − 0 37 1 0 CGCGAATGCCCTTCCAAGACCGTCACAAAAAGG 1053 CCGCTGTTGTTTACGCGTGGCGGATGT PDGFRA NM_006206 3 4 + 210 + 0 37 1 0 CGCGAATGCCTGAGCAGTGCCTCGGCGGCCCAC 1054 ACAGGGTTGTACACGCGTGGCGGATGT PDGFRA NM_006206 3 4 + 245 − 0 37 1 0 CGCGAATGCCTTCTCTTCTGTCTGAGTGTGGTTG 1055 TAATAGCAAGTACGCGTGGCGGATGT PDGFRA NM_006206 3 4 + 280 + 0 37 1 0 CGCGAATGCCTGAGCTTGAAGGCAGGCACATTT 1056 ACATCTATGTGCACGCGTGGCGGATGT PDGFRA NM_006206 3 4 + 315 − 0 37 1 0 CGCGAATGCCGAAGCTTGGTCCTGGAGACCCAG 1057 CCAACTCACCTGACGCGTGGCGGATGT EPHA7 NM_004440 16 6 − −38 + 0 37 1 0 CGCGAATGCCTTTCATGTAACATATGGAAATAC 1058 AACTTTCTTTTTACGCGTGGCGGATGT EPHA7 NM_004440 16 6 − −3 − 0 37 1 0 CGCGAATGCCTGTTTGTTGTGCTTTAGAATCCAG 1059 CAGTAGTACTGACGCGTGGCGGATGT EPHA7 NM_004440 16 6 − 32 + 0 37 1 0 CGCGAATGCCGAGTTGGAGTGGATTTCCTCTCC 1060 ACCCAATGGGGTACGCGTGGCGGATGT EPHA7 NM_004440 16 6 − 67 − 0 37 1 0 CGCGAATGCCTTTAAAAAAGTGATATTTTATGA 1061 TGAAAAAAACTTACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 0 + 0 37 1 0 CGCGAATGCCAATGAAGAGGTCCATGATGAGGC 1062 CGAAGAGTCAGAACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 35 − 0 37 1 0 CGCGAATGCCAGAGGAGGGACAGGTTCATCTCC 1063 TCGAAGTCATCCACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 70 + 0 37 1 0 CGCGAATGCCCGGCCCGGAGCTTCCCACGCAAG 1064 GCCAGCCAGACCACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 105 − 0 37 1 0 CGCGAATGCCTCAAAGGGGATGTCCCACTCCTG 1065 AAGGAAGATGCTACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 140 + 0 37 1 0 CGCGAATGCCGCAGCTGGAGATCGGCGAGCTCA 1066 TTGGAAAGGGCCACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 175 − 0 37 1 0 CGCGAATGCCGCCATGCCAGCGGCCGTGGTACA 1067 CTTGCCCAAAGCACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 210 + 0 37 1 0 CGCGAATGCCGAGGTGGCCATCCGGCTGATTGA 1068 CATTGAGAGGGAACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 245 − 0 37 1 0 CGCGAATGCCCCTCCCGCTTGAAGGCCTTGAGC 1069 TGGTCCTCGTTGACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 280 + 0 37 1 0 CGCGAATGCCTGATGGCCTACAGGCAGACACGG 1070 CATGAGAACGTGACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 315 − 0 37 1 0 CGCGAATGCCTGAGGCGGGCTCATGCAGGCACC 1071 CATGAAAAGCACACGCGTGGCGGATGT KSR2 NM_173598 7 12 − 350 + 0 37 1 0 CGCGAATGCCCCTGGCCATCATCACCAGGTCAG 1072 TTCCACCTGGGCACGCGTGGCGGATGT GUCY2F NM_001522 9 X − 0 + 0 37 1 0 CGCGAATGCCAAATCATAAACAGACTTAAGAAG 1073 CCTCCTCCTGTGACGCGTGGCGGATGT GUCY2F NM_001522 9 X − 35 − 0 37 1 0 CGCGAATGCCGGAGGGGCATGCTCAGGAGGAA 1074 CTACTGGTCTGTAACGCGTGGCGGATGT GUCY2F NM_001522 9 X − 70 + 0 37 1 0 CGCGAATGCCAGAATGTCTCCAGCTGATGAAGC 1075 AGTGCTGGGCTGACGCGTGGCGGATGT GUCY2F NM_001522 9 X − 105 − 0 37 1 0 CGCGAATGCCTATTTCATCAAAAGTTGGTCGTTG 1076 TTCTGCAGCCTACGCGTGGCGGATGT GUCY2F NM_001522 9 X − 140 + 0 37 1 0 CGCGAATGCCTTTAACCAGGTAAGGACTCTGAA 1077 TCTTATCATTGCACGCGTGGCGGATGT RBBP8 NM_002894 6 18 + −36 + 0 37 1 0 CGCGAATGCCTATATTATTTGCCTTCTTTTTCAC 1078 ATTGTTTTTAAACGCGTGGCGGATGT RBBP8 NM_002894 6 18 + −1 − 0 37 1 0 CGCGAATGCCTTATTTTCTTCCTGTAGAGTATTC 1079 CTTTCATTCACACGCGTGGCGGATGT RBBP8 NM_002894 6 18 + 34 + 0 37 1 0 CGCGAATGCCAAAGCTTTCTGAACAACTCCAGC 1080 AGAAAATTGAGTACGCGTGGCGGATGT RBBP8 NM_002894 6 18 + 69 − 0 37 1 0 CGCGAATGCCATAAAATAAAATAACAAGGTTGG 1081 AGGAAAATACTTACGCGTGGCGGATGT RB1 NM_000321 1 13 + −6 + 2 37 1 0 CGCGAATGCCGGCGTCATGCCGCCCAAAACCCC 1082 CCGAAAAACGGCACGCGTGGCGGATGT RB1 NM_000321 1 13 + 33 − 35 37 1 0 CGCGAATGCCGGTGCCGGGGGTTCCGCGGCGGC 1083 AGCGGCGGCGGTACGCGTGGCGGATGT RB1 NM_000321 1 13 + 72 + 8 37 1 0 CGCGAATGCCCCGCCGCCCCCTCCTGAGGAGGA 1084 CCCAGAGCAGGAACGCGTGGCGGATGT RB1 NM_000321 1 13 + 103 − 0 37 1 0 CGCGAATGCCCCTGACGAGAGGCAGGTCCTCCG 1085 GGCCGCTGTCCTACGCGTGGCGGATGT PDGFRA NM_006206 14 4 + −14 + 0 37 1 0 CGCGAATGCCTATTCTTTCAACAGCCACGGCCA 1086 GATCCAGTGAAAACGCGTGGCGGATGT PDGFRA NM_006206 14 4 + 21 − 0 37 1 0 CGCGAATGCCAGTCATTATCTTCAGTTCAGACAT 1087 GAGAGCTTGTTACGCGTGGCGGATGT PDGFRA NM_006206 14 4 + 56 + 0 37 1 0 CGCGAATGCCCACCTGGGGCCACATTTGAACAT 1088 TGTAAACTTGCTACGCGTGGCGGATGT PDGFRA NM_006206 14 4 + 91 − 0 37 1 0 CGCGAATGCCGGTCAGTGAGCCCACCTGACTTG 1089 GTGCAGGCTCCCACGCGTGGCGGATGT CHAF1A NM_005483 5 19 + 0 + 0 37 1 0 CGCGAATGCCGATCAGGAGCGTCTGGGCAAGCA 1090 GCTCAAGTTACGACGCGTGGCGGATGT CHAF1A NM_005483 5 19 + 35 − 0 37 1 0 CGCGAATGCCCCTCTTTCAGCTTCTCCTTTTCTTC 1091 CCTTTCTGCAACGCGTGGCGGATGT CHAF1A NM_005483 5 19 + 66 + 4 37 1 0 CGCGAATGCCGAGGAGGCCAAGCGGGCCAAGG 1092 AGGAGGCCAAGAAACGCGTGGCGGATGT CHAF1A NM_005483 5 19 + 105 − 35 37 1 0 CGCGAATGCCCTCTCCTTTTCCTTAAGCTCCTTC 1093 TCTTCCTCCTTACGCGTGGCGGATGT CHAF1A NM_005483 5 19 + 144 + 31 37 1 0 CGCGAATGCCGAGAAGCGGGAGAAGGATGAGA 1094 AGGAGAAGGCGGAACGCGTGGCGGATGT CHAF1A NM_005483 5 19 + 175 − 0 37 1 0 CGCGAATGCCCTTGCGCCGCTCCTCCTTGAGCCG 1095 CTGCTTCTCCGACGCGTGGCGGATGT CHAF1A NM_005483 5 19 + 210 + 0 37 1 0 CGCGAATGCCGAGAGACAGGAAGCCCTGGAGT 1096 GAGTGTCCTTGGAACGCGTGGCGGATGT NFKB1 NM_003998 2 4 + −54 + 17 37 1 0 CGCGAATGCCGTTCATTCTAGTGTTACAGTTTTG 1097 TTTTGTTTTGTACGCGTGGCGGATGT NFKB1 NM_003998 2 4 + −15 − 0 37 1 0 CGCGAATGCCTATGGATCATCTTCTGCCATTCTG 1098 AAGCTGTGTATACGCGTGGCGGATGT NFKB1 NM_003998 2 4 + 20 + 0 37 1 0 CGCGAATGCCTTTGGGAAGGCCTGAACAAGTAA 1099 GTGTCATAATCTACGCGTGGCGGATGT NFKB1 NM_003998 2 4 + 55 − 0 37 1 0 CGCGAATGCCTGAAATATGAATATATTTAAATA 1100 AAGTTATCAGTGACGCGTGGCGGATGT RET NM_020630 11 10 + 0 + 0 37 1 0 CGCGAATGCCATCCACTGTGCGACGAGCTGTGC 1101 CGCACGGTGATCACGCGTGGCGGATGT RET NM_020630 11 10 + 35 − 0 37 1 0 CGCGAATGCCACCGAGACGATGAAGGAGAAGA 1102 GGACAGCGGCTGCACGCGTGGCGGATGT RET NM_020630 11 10 + 70 + 0 37 1 0 CGCGAATGCCGCTGCTGTCTGCCTTCTGCATCCA 1103 CTGCTACCACAACGCGTGGCGGATGT RET NM_020630 11 10 + 105 − 0 37 1 0 CGCGAATGCCCTCAGCTGAGGAGATGGGTGGCT 1104 TGTGGGCAAACTACGCGTGGCGGATGT RET NM_020630 11 10 + 140 + 0 37 1 0 CGCGAATGCCATGACCTTCCGGAGGCCCGCCCA 1105 GGCCTTCCCGGTACGCGTGGCGGATGT RET NM_020630 11 10 + 175 − 0 37 1 0 CGCGAATGCCGCGAGGGCCGGCGGGCACCGGA 1106 AGAGGAGTAGCTGACGCGTGGCGGATGT RET NM_020630 11 10 + 210 + 0 37 1 0 CGCGAATGCCTGGACTCCATGGAGAACCAGGTC 1107 TCCGTGGATGCCACGCGTGGCGGATGT RET NM_020630 11 10 + 245 − 0 37 1 0 CGCGAATGCCCCCTGCCCCGCAGGGACCCTCAC 1108 CAGGATCTTGAAACGCGTGGCGGATGT NFKB1 NM_003998 15 4 + 0 + 0 37 1 0 CGCGAATGCCATAACCTCTTTCTAGAGAAGGCT 1109 ATGCAGCTTGCAACGCGTGGCGGATGT NFKB1 NM_003998 15 4 + 35 − 0 37 1 0 CGCGAATGCCACCGCGTAGTCGAAAAGGGCATT 1110 GGCATGCCTCTTACGCGTGGCGGATGT NFKB1 NM_003998 15 4 + 70 + 0 37 1 0 CGCGAATGCCGACAGGAGACGTGAAGATGCTGC 1111 TGGCCGTCCAGCACGCGTGGCGGATGT NFKB1 NM_003998 15 4 + 105 − 0 37 1 0 CGCGAATGCCGTCCCCATTCTCATCCTGCACAGC 1112 AGTGAGATGGCACGCGTGGCGGATGT NFKB1 NM_003998 15 4 + 140 + 0 37 1 0 CGCGAATGCCAGGTAAGTCAGAACTTTTGCATG 1113 ATAGGTTGTCCTACGCGTGGCGGATGT NTRK3 NM_001012338 16 15 − −34 + 0 37 1 0 CGCGAATGCCGAGCTCTGCTGATCCTCTTTTTCT 1114 CTCTGTCTAGGACGCGTGGCGGATGT NTRK3 NM_001012338 16 15 − 1 − 0 37 1 0 CGCGAATGCCGGCTGAATGCTCCGAAGTCCTGA 1115 GTTCTTGATGGTACGCGTGGCGGATGT NTRK3 NM_001012338 16 15 − 36 + 0 37 1 0 CGCGAATGCCCAGAGCCTTTGCCAAGAACCCCC 1116 ATTTGCGTTATAACGCGTGGCGGATGT NTRK3 NM_001012338 16 15 − 71 − 0 37 1 0 CGCGAATGCCGTGCCCCCAATCCCTGCAGCCCA 1117 GCTCTACTCACAACGCGTGGCGGATGT NFKB1 NM_003998 9 4 + −18 + 0 37 1 0 CGCGAATGCCTTTTTCCCCTGTGAACAGAAGCC 1118 CCCAATGCATCCACGCGTGGCGGATGT NFKB1 NM_003998 9 4 + 17 − 0 37 1 0 CGCGAATGCCCCAGCTGTCCTGTCCATTCTTACA 1119 ATTTTCAAGTTACGCGTGGCGGATGT NFKB1 NM_003998 9 4 + 52 + 0 37 1 0 CGCGAATGCCATGTGTGACTGGAGGGGAGGAA 1120 ATTTATCTTCTTTACGCGTGGCGGATGT NFKB1 NM_003998 9 4 + 87 − 0 37 1 0 CGCGAATGCCTCACAGAATGTATTTACCTTTCTG 1121 AACTTTGTCACACGCGTGGCGGATGT CENTG1 NM_014770 13 12 − −2 + 0 37 1 0 CGCGAATGCCAGACAGGATCAGTGCTTCCTCCC 1122 CTCGGGTGGTGGACGCGTGGCGGATGT CENTG1 NM_014770 13 12 − 33 − 0 37 1 0 CGCGAATGCCCATGTCCGCGCACAGAGCTCTGG 1123 CACGAGCATCTCACGCGTGGCGGATGT CENTG1 NM_014770 13 12 − 68 + 0 37 1 0 CGCGAATGCCAAACGCTGCAGCTACTATGAGAC 1124 TTGTGCAACCTAACGCGTGGCGGATGT CENTG1 NM_014770 13 12 − 103 − 0 37 1 0 CGCGAATGCCCACCCTCCTGGAAGACCCGATCC 1125 ACATTGAGCCCAACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 0 + 0 37 1 0 CGCGAATGCCTGGGAGGAAGTGAGTATCATGGA 1126 TGAAAAAAATACACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 35 − 0 37 1 0 CGCGAATGCCCCATCACATTGCACACTTGGTAG 1127 GTTCGGATTGGTACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 70 + 0 37 1 0 CGCGAATGCCAACCCAGCCAGAATAACTGGCTA 1128 CGAACTGATTGGACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 105 − 0 37 1 0 CGCGAATGCCTCAATATACACCCTCTGAGCCCC 1129 TTCTCGGGTGATACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 140 + 0 37 1 0 CGCGAATGCCGATTAAATTCACCTTGAGGGACT 1130 GCAATAGTCTTCACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 175 − 0 37 1 0 CGCGAATGCCGTTAAACGTCTCCTTGCAAGTCC 1131 CCATGACGCCCGACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 210 + 0 37 1 0 CGCGAATGCCCTGTACTACTATGAATCAGACAA 1132 CGACAAAGAGCGACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 245 − 0 37 1 0 CGCGAATGCCTGTCAATTTTGACAAACTGGTTCT 1133 CTCTGATGAAAACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 280 + 0 37 1 0 CGCGAATGCCCCATTGCTGCTGATGAGAGCTTC 1134 ACCCAAGTGGACACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 315 − 0 37 1 0 CGCGAATGCCATCTCGGTGTTCAGCTTCATGATT 1135 CTGTCACCAATACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 350 + 0 37 1 0 CGCGAATGCCCCGGGATGTAGGGCCATTAAGCA 1136 AAAAGGGGTTTTACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 385 − 0 37 1 0 CGCGAATGCCGGCGATGCAGGCCCCCACATCCT 1137 GAAAAGCCAGGTACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 420 + 0 37 1 0 CGCGAATGCCCTGGTATCAGTCCGTGTGTTCTAT 1138 AAAAAGTGTCCACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 455 − 0 37 1 0 CGCGAATGCCTGTCAGGAAACTGGGCCAGATTG 1139 CGGACTGTGAGTACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 490 + 0 37 1 0 CGCGAATGCCCCATCACAGGGGCTGATACGTCT 1140 TCCCTGGTGGAAACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 525 − 0 37 1 0 CGCGAATGCCTTCTCTTCTGAGTTGTTGACACAG 1141 GAGCCTCGAACACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 560 + 0 37 1 0 CGCGAATGCCAGATGTGCCAAAAATGTACTGTG 1142 GGGCAGATGGTGACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 595 − 0 37 1 0 CGCGAATGCCGTTGCATAGGCAGTTGCCAATGG 1143 GTACCAGCCATTACGCGTGGCGGATGT EPHA4 NM_004438 16 2 − 630 + 0 37 1 0 CGCGAATGCCGCTGGGCATGAGGAGCGGAGCG 1144 GAGAATGCCAAGGACGCGTGGCGGATGT CENTG1 NM_014770 12 12 − −15 + 0 37 1 0 CGCGAATGCCGTCAACTCCCCTCAGTGGCCCAG 1145 AAGGTGGTGACCACGCGTGGCGGATGT CENTG1 NM_014770 12 12 − 20 − 0 37 1 0 CGCGAATGCCTTGCAGGCAGCCAGAAGCTGTTG 1146 CTGCTTGCGCAAACGCGTGGCGGATGT CENTG1 NM_014770 12 12 − 55 + 0 37 1 0 CGCGAATGCCGTCCCTGCCCAGCTCCCCAAGCC 1147 ACTCAGCTGCATACGCGTGGCGGATGT CENTG1 NM_014770 12 12 − 90 − 0 37 1 0 CGCGAATGCCGAAACCCCAACTCACCTGGCCAG 1148 CTACCGGAGTGGACGCGTGGCGGATGT PKN1 NM_002741 16 19 + 0 + 0 37 1 0 CGCGAATGCCCCTGATGTGTGAGAAGCGGATAT 1149 TGGCGGCAGTGAACGCGTGGCGGATGT PKN1 NM_002741 16 19 + 35 − 0 37 1 0 CGCGAATGCCGAAGAGGTTCACCAGGAAGGGG 1150 TGTCCCGCACTGGACGCGTGGCGGATGT PKN1 NM_002741 16 19 + 70 + 0 37 1 0 CGCGAATGCCGGCTGTTTCCAGACACCGGAGCA 1151 CGTGTGCTTCGTACGCGTGGCGGATGT PKN1 NM_002741 16 19 + 105 − 0 37 1 0 CGCGAATGCCGCAGCATCAGGTCCCCACCGGCC 1152 GAGTACTCCATCACGCGTGGCGGATGT PKN1 NM_002741 16 19 + 140 + 0 37 1 0 CGCGAATGCCACATCCACAGCGACGTGTTCTCT 1153 GAGCCCCGTGCCACGCGTGGCGGATGT PKN1 NM_002741 16 19 + 175 − 0 37 1 0 CGCGAATGCCGAGGCAGGGGCCCGGGCTGGGG 1154 TCCAGGCTCACATACGCGTGGCGGATGT GUCY2F NM_001522 8 X − 0 + 0 37 1 0 CGCGAATGCCTTTAAAACTTTTAATAAAGGGAA 1155 GAAGACCAATATACGCGTGGCGGATGT GUCY2F NM_001522 8 X − 35 − 0 37 1 0 CGCGAATGCCAATATTGCTCCAACATCCGAAGC 1156 ATAGAATCAATAACGCGTGGCGGATGT GUCY2F NM_001522 8 X − 70 + 0 37 1 0 CGCGAATGCCCTAGCAACTTGGAAGATTTGATT 1157 CGGGAGCGGACTACGCGTGGCGGATGT GUCY2F NM_001522 8 X − 105 − 0 37 1 0 CGCGAATGCCTTTTCCGTTTTCTGTTTTTCAATTT 1158 CCAGCTCTTCACGCGTGGCGGATGT GUCY2F NM_001522 8 X − 140 + 0 37 1 0 CGCGAATGCCGCTTCTAACACAGATGCTACCAC 1159 CGTATGTGAGAAACGCGTGGCGGATGT RET NM_020630 19 10 + 0 + 0 37 1 0 CGCGAATGCCGACTACTTGGACCTTGCGGCGTC 1160 CACTCCATCTGAACGCGTGGCGGATGT RET NM_020630 19 10 + 35 − 0 37 1 0 CGCGAATGCCCCTCCTCTGAGAGGCCGTCGTCA 1161 TAAATCAGGGAGACGCGTGGCGGATGT RET NM_020630 19 10 + 70 + 0 37 1 0 CGCGAATGCCAGACACCGCTGGTGGACTGTAAT 1162 AATGCCCCCCTCACGCGTGGCGGATGT RET NM_020630 19 10 + 105 − 0 37 1 0 CGCGAATGCCTTGTTTTCAATCCATGTGGAAGG 1163 GAGGGCTCGAGGACGCGTGGCGGATGT RET NM_020630 19 10 + 140 + 0 37 1 0 CGCGAATGCCACTCTATGGTAGAATTTCCCATG 1164 CATTTACTAGATACGCGTGGCGGATGT RET NM_020630 19 10 + 175 − 0 37 1 0 CGCGAATGCCAGGAAGGATAGTGCAAAGGGGA 1165 CAGCGGTGCTAGAACGCGTGGCGGATGT RB1 NM_000321 12 13 + −26 + 0 37 1 0 CGCGAATGCCCATTTTCCTATTTTTATCCCCTCT 1166 AGGACTGTTATACGCGTGGCGGATGT RB1 NM_000321 12 13 + 9 − 0 37 1 0 CGCGAATGCCAATTTAAAATCATCATTAATTGTT 1167 GGATAGTGTTCACGCGTGGCGGATGT RB1 NM_000321 12 13 + 44 + 0 37 1 0 CGCGAATGCCCAGCAAGTGATCAACCTTCAGAA 1168 AATCTGATTTCCACGCGTGGCGGATGT RB1 NM_000321 12 13 + 79 − 0 37 1 0 CGCGAATGCCTAAATAATGTTTCATATATGGCTT 1169 ACGTTAAAATAACGCGTGGCGGATGT GUCY2F NM_001522 14 X − −4 + 0 37 1 0 CGCGAATGCCACAGAGAGGAAGTCGTGCCAGTG 1170 TAAGCTTCCAGAACGCGTGGCGGATGT GUCY2F NM_001522 14 X − 31 − 0 37 1 0 CGCGAATGCCTCTTGGGGACCTCCCACTTTGGA 1171 CCTCTGAGGTAAACGCGTGGCGGATGT GUCY2F NM_001522 14 X − 66 + 0 37 1 0 CGCGAATGCCCTCTCCTTTTCTTCAGGGAGTCTA 1172 ACTCCAGCTACACGCGTGGCGGATGT GUCY2F NM_001522 14 X − 101 − 0 37 1 0 CGCGAATGCCTTACCTCATAAATCGCTATGTTGG 1173 AGTTTTCATAGACGCGTGGCGGATGT RET NM_020975 20 10 + 0 + 0 37 1 0 CGCGAATGCCGCATGTCAGACCCGAACTGGCCT 1174 GGAGAGAGTCCTACGCGTGGCGGATGT RET NM_020975 20 10 + 35 − 0 37 1 0 CGCGAATGCCCCAGTGTTAGTGCCATCAGCTCT 1175 CGTGAGTGGTACACGCGTGGCGGATGT RET NM_020975 20 10 + 70 + 0 37 1 0 CGCGAATGCCGTTTCCAAGATATCCAAATGATA 1176 GTGTATATGCTAACGCGTGGCGGATGT RET NM_020975 20 10 + 105 − 0 37 1 0 CGCGAATGCCCATTAATTTTGCCGCTGAGGGTG 1177 AAAGCATCCAGTACGCGTGGCGGATGT RET NM_020975 20 10 + 140 + 0 37 1 0 CGCGAATGCCGACACGTTTGATAGTTAACATTT 1178 CTTTGTGAAAGGACGCGTGGCGGATGT CHAF1A NM_005483 6 19 + −40 + 0 37 1 0 CGCGAATGCCAAGCAAAGAGTCGGCTGAAATGT 1179 CATTTGCTGTCTACGCGTGGCGGATGT CHAF1A NM_005483 6 19 + −5 − 0 37 1 0 CGCGAATGCCTCCTTTTTCCTTTTTTCCTCAAGTT 1180 TAGCCCTGTGACGCGTGGCGGATGT CHAF1A NM_005483 6 19 + 30 + 0 37 1 0 CGCGAATGCCAGAAGAGAAACGGTTAAGAGAA 1181 GAAGAGAAGGTAGACGCGTGGCGGATGT CHAF1A NM_005483 6 19 + 65 − 0 37 1 0 CGCGAATGCCACGGGCTGGGACGGGGAAGCTCT 1182 GTGGGAAACACTACGCGTGGCGGATGT KSR2 NM_173598 14 12 − −28 + 0 37 1 0 CGCGAATGCCTTGAACATTTTCTGTCTCTTTTCC 1183 ACAGGTTTTCCACGCGTGGCGGATGT KSR2 NM_173598 14 12 − 7 − 0 37 1 0 CGCGAATGCCCAGACTGTGCACGTCTGAGACAT 1184 CCAGTACTTGGTACGCGTGGCGGATGT KSR2 NM_173598 14 12 − 42 + 0 37 1 0 CGCGAATGCCTGGGAAAGGGATGCTTTTTGGCC 1185 TCAAGTGTAAAAACGCGTGGCGGATGT KSR2 NM_173598 14 12 − 77 − 0 37 1 0 CGCGAATGCCAAAGTCACTGCAGGGCACAGTCA 1186 CTTACTTGCAGTACGCGTGGCGGATGT EPHA7 NM_004440 9 6 − −42 + 0 37 1 0 CGCGAATGCCAAGTAAAATGACTGAGATTGTCA 1187 CAAATTTGCTTTACGCGTGGCGGATGT EPHA7 NM_004440 9 6 − −7 − 0 37 1 0 CGCGAATGCCTTGGTCAGCTTTGCTATAACCAC 1188 AGTGCCTTGAAGACGCGTGGCGGATGT EPHA7 NM_004440 9 6 − 28 + 0 37 1 0 CGCGAATGCCGAAGGCGATGAAGAGCTTTACTT 1189 TCATTGTAAGTGACGCGTGGCGGATGT EPHA7 NM_004440 9 6 − 63 − 0 37 1 0 CGCGAATGCCTTAATAGGGGTACATCATAATAA 1190 AGAAAAGCCAAAACGCGTGGCGGATGT RPS6KA1 NM_002953 10 1 + −34 + 0 37 1 0 CGCGAATGCCGATCAGAGCCTGAATAGATCCTT 1191 GTCCTCTGCAGTACGCGTGGCGGATGT RPS6KA1 NM_002953 10 1 + 1 − 0 37 1 0 CGCGAATGCCCCCCTGGAAGGGCAGGGAGCCCG 1192 TCAGCATCTCAAACGCGTGGCGGATGT RPS6KA1 NM_002953 10 1 + 36 + 0 37 1 0 CGCGAATGCCAAGGACCGGAAGGAGACCATGA 1193 CACTGATTCTGAAACGCGTGGCGGATGT RPS6KA1 NM_002953 10 1 + 71 − 0 37 1 0 CGCGAATGCCGAGTCCATTGTTATCAGGGCAGG 1194 GCTGGGGCTTACACGCGTGGCGGATGT EPHA3 NM_005233 5 3 + 0 + 0 37 1 0 CGCGAATGCCGACCTCCATCTTCACCAAGAAAT 1195 GTTATCTCTAATACGCGTGGCGGATGT EPHA3 NM_005233 5 3 + 35 − 0 37 1 0 CGCGAATGCCCAACTCCAGTCCAGGATAACTGA 1196 GGTCTCGTTTATACGCGTGGCGGATGT EPHA3 NM_005233 5 3 + 70 + 0 37 1 0 CGCGAATGCCGCCCCTGGACACAGGAGGCCGGA 1197 AAGATGTTACCTACGCGTGGCGGATGT EPHA3 NM_005233 5 3 + 105 − 0 37 1 0 CGCGAATGCCTATATTCCACCCACATTTTTTACA 1198 TATGATGTTGAACGCGTGGCGGATGT EPHA3 NM_005233 5 3 + 140 + 0 37 1 0 CGCGAATGCCAAACAGTGTGAGCCATGCAGCCC 1199 AAATGTCCGCTTACGCGTGGCGGATGT EPHA3 NM_005233 5 3 + 175 − 0 37 1 0 CGCGAATGCCCCGTGGTGTTGGTGAGTCCAAAC 1200 TGTCGAGGGAGGACGCGTGGCGGATGT EPHA3 NM_005233 5 3 + 210 + 0 37 1 0 CGCGAATGCCTGACAGTGACAGACCTTCTGGCA 1201 CATACTAACTACACGCGTGGCGGATGT EPHA3 NM_005233 5 3 + 245 − 0 37 1 0 CGCGAATGCCTCTGACACCCCATTAACGGCATC 1202 AATCTCAAAGGTACGCGTGGCGGATGT EPHA3 NM_005233 5 3 + 280 + 0 37 1 0 CGCGAATGCCGCTGAGCTCCCCACCAAGACAGT 1203 TTGCTGCGGTCAACGCGTGGCGGATGT EPHA3 NM_005233 5 3 + 315 − 0 37 1 0 CGCGAATGCCTAGTATGTACTCACCAGCCTGAT 1204 TAGTTGTGATGCACGCGTGGCGGATGT RET NM_020630 10 10 + −10 + 0 37 1 0 CGCGAATGCCCTGCCCTCAGGGGGCAGCATTGT 1205 TGGGGGACACGAACGCGTGGCGGATGT RET NM_020630 10 10 + 25 − 0 37 1 0 CGCGAATGCCCATAGCCAGCTTTAATCCCCCGG 1206 GGCTCCCCAGGCACGCGTGGCGGATGT RET NM_020630 10 10 + 60 + 0 37 1 0 CGCGAATGCCGCACCTGCAACTGCTTCCCTGAG 1207 GAGGAGAAGTGCACGCGTGGCGGATGT RET NM_020630 10 10 + 95 − 0 37 1 0 CGCGAATGCCACCCACTCACCCTGGATGTCTTC 1208 GGGCTCGCAGAAACGCGTGGCGGATGT NFKB1 NM_003998 13 4 + −25 + 0 37 1 0 CGCGAATGCCTCATTTGTTTTACTTGCCGTTTCA 1209 GGGTATAGCTTACGCGTGGCGGATGT NFKB1 NM_003998 13 4 + 10 − 0 37 1 0 CGCGAATGCCTAATCCCACCATAAGTAGGAAAT 1210 CCATAGTGTGGGACGCGTGGCGGATGT NFKB1 NM_003998 13 4 + 45 + 0 37 1 0 CGCGAATGCCCTTTCCATCCTGGAACTACTAAAT 1211 CTAATGCTGGGACGCGTGGCGGATGT NFKB1 NM_003998 13 4 + 80 − 0 37 1 0 CGCGAATGCCGTATTAAGAACAAAGCATTACTT 1212 ACCATGCTTCATACGCGTGGCGGATGT RB1 NM_000321 18 13 + −10 + 0 37 1 0 CGCGAATGCCTTTCATATAGGATTCACCTTTATT 1213 TGATCTTATTAACGCGTGGCGGATGT RB1 NM_000321 18 13 + 25 − 0 37 1 0 CGCGAATGCCGTGATCAGTTGGTCCTTCTCGGTC 1214 CTTTGATTGTTACGCGTGGCGGATGT RB1 NM_000321 18 13 + 60 + 0 37 1 0 CGCGAATGCCCTTGAATCTGCTTGTCCTCTTAAT 1215 CTTCCTCTCCAACGCGTGGCGGATGT RB1 NM_000321 18 13 + 95 − 0 37 1 0 CGCGAATGCCATTTTGCTTACATATCTGCTGCAG 1216 TGTGATTATTCACGCGTGGCGGATGT GUCY2F NM_001522 5 X − −20 + 0 37 1 0 CGCGAATGCCAACCCCCTGATACTCTATAGGGC 1217 CGGTTGTTGCTGACGCGTGGCGGATGT GUCY2F NM_001522 5 X − 15 − 0 37 1 0 CGCGAATGCCCAAGCAGTATCTGGGCATGGTGA 1218 GGCCCACCACTCACGCGTGGCGGATGT GUCY2F NM_001522 5 X − 50 + 0 37 1 0 CGCGAATGCCTTTGGAGACACTGTGAACACAGC 1219 TTCTCGGATGGAACGCGTGGCGGATGT GUCY2F NM_001522 5 X − 85 − 0 37 1 0 CGCGAATGCCATCTCTCTATTAGGTACTCACGTA 1220 AGCCTGTAGATACGCGTGGCGGATGT EPHA7 NM_004440 5 6 − 0 + 0 37 1 0 CGCGAATGCCAAACATGATGGGCAATTTACAGT 1221 CATTCAGTTAGTACGCGTGGCGGATGT EPHA7 NM_004440 5 6 − 35 − 0 37 1 0 CGCGAATGCCATCTCATTCCAGCAGCAATTCCTC 1222 TCAGCATTCCTACGCGTGGCGGATGT EPHA7 NM_004440 5 6 − 70 + 0 37 1 0 CGCGAATGCCATTTGGCTGATATGGGATATGTT 1223 CACAGGGACCTTACGCGTGGCGGATGT EPHA7 NM_004440 5 6 − 105 − 0 37 1 0 CGCGAATGCCACGAGATTGCTGTTGACAAGAAT 1224 ATTGCGAGCTGCACGCGTGGCGGATGT EPHA7 NM_004440 5 6 − 140 + 0 37 1 0 CGCGAATGCCTTGTAAAGTGTCAGATTTTGGCCT 1225 GTCCCGAGTTAACGCGTGGCGGATGT EPHA7 NM_004440 5 6 − 175 − 0 37 1 0 CGCGAATGCCAGTAGTTGTATAGACAGCTTCTG 1226 GATCATCCTCTAACGCGTGGCGGATGT EPHA7 NM_004440 5 6 − 210 + 0 37 1 0 CGCGAATGCCGTAAGAAAAAGTCATTTACTGCA 1227 CTTCTTCATATTACGCGTGGCGGATGT NFKB1 NM_003998 24 4 + 0 + 0 37 1 0 CGCGAATGCCACGAGCTCCGAGACAGTGACAGT 1228 GTCTGCGACAGCACGCGTGGCGGATGT NFKB1 NM_003998 24 4 + 35 − 0 37 1 0 CGCGAATGCCGTAAAGCTGAGTTTGCGGAAGGA 1229 TGTCTCCACGCCACGCGTGGCGGATGT NFKB1 NM_003998 24 4 + 70 + 0 37 1 0 CGCGAATGCCCGAGTCTCTGACCAGTGGTGCCT 1230 CACTGCTAACTCACGCGTGGCGGATGT NFKB1 NM_003998 24 4 + 105 − 0 37 1 0 CGCGAATGCCTCCTTCCTGCCCATAATCATGGG 1231 GCATTTTGTTGAACGCGTGGCGGATGT NFKB1 NM_003998 24 4 + 140 + 0 37 1 0 CGCGAATGCCCCTCTAGAAGGCAAAATTTAGCC 1232 TGCTGACAATTTACGCGTGGCGGATGT RBBP8 NM_203292 18 18 + −18 + 0 37 1 0 CGCGAATGCCTTATGATTTGTTTTTAAGGTTATA 1233 TTAAGGAAGATACGCGTGGCGGATGT RBBP8 NM_203292 18 18 + 17 − 0 37 1 0 CGCGAATGCCGGCTGACGTCTTTTTGGACGAGG 1234 ACAAGGATCAAGACGCGTGGCGGATGT RBBP8 NM_203292 18 18 + 52 + 0 37 1 0 CGCGAATGCCTTACAACGCAATATTTTCTCCAA 1235 AAGGCAAGGAGCACGCGTGGCGGATGT RBBP8 NM_203292 18 18 + 87 − 0 37 1 0 CGCGAATGCCATCCTTCTGTTTCTGTTTCAACGT 1236 CTATGTCTTCTACGCGTGGCGGATGT EPHA4 NM_004438 2 2 − −12 + 0 37 1 0 CGCGAATGCCCCCTATGTACAGGGACCTGGCAA 1237 GAATTGGTATCAACGCGTGGCGGATGT EPHA4 NM_004438 2 2 − 23 − 0 37 1 0 CGCGAATGCCACTGCTCAAAATCTTATTCTGGTG 1238 CGTGATGGCTGACGCGTGGCGGATGT EPHA4 NM_004438 2 2 − 58 + 0 37 1 0 CGCGAATGCCGTCCAGGCAATGCGAACCCAAAT 1239 GCAGCAGATGCAACGCGTGGCGGATGT EPHA4 NM_004438 2 2 − 93 − 0 37 1 0 CGCGAATGCCATTCAGTACTGGCTCAGACGGGA 1240 ACCATTCTGCCGACGCGTGGCGGATGT RPS6KA1 NM_002953 6 1 + 0 + 0 37 1 0 CGCGAATGCCTACGTGACCGCGTCCGGACCAAG 1241 ATGGAGAGAGACACGCGTGGCGGATGT RPS6KA1 NM_002953 6 1 + 35 − 0 37 1 0 CGCGAATGCCTTCACCACGAATGGGTGATTTAC 1242 ATCAGCCAGGATACGCGTGGCGGATGT RPS6KA1 NM_002953 6 1 + 70 + 0 37 1 0 CGCGAATGCCGCTGCACTATGGTAAAGCTTCTG 1243 GCCCTGCCTGAGACGCGTGGCGGATGT RPS6KA1 NM_002953 6 1 + 105 − 0 37 1 0 CGCGAATGCCCCACAGGCAAGGGCGAAGGATG 1244 GGTGGGGTAGGAGACGCGTGGCGGATGT RPS6KA1 NM_002953 6 1 + 140 + 0 37 1 0 CGCGAATGCCTCTGTACACTGTCCCACCGCCTGC 1245 CTGGCAGGCCAACGCGTGGCGGATGT RPS6KA1 NM_002953 6 1 + 175 − 0 37 1 0 CGCGAATGCCGGCCTTATGATCTGCTTCTCCGGC 1246 CCTGGCTCCCTACGCGTGGCGGATGT RPS6KA1 NM_002953 6 1 + 210 + 0 37 1 0 CGCGAATGCCGCGCCGACTCTACCATTGCCTTTC 1247 TCCCTCTTCCCACGCGTGGCGGATGT RPS6KA1 NM_002953 6 1 + 245 − 0 37 1 0 CGCGAATGCCGAATGAGATAGAGCTTGCCCTCG 1248 GTCTGGAAGGCTACGCGTGGCGGATGT RPS6KA1 NM_002953 6 1 + 280 + 0 37 1 0 CGCGAATGCCTGGACTTCCTGCGTGGTGGGGAC 1249 CTCTTCACCCGGACGCGTGGCGGATGT RPS6KA1 NM_002953 6 1 + 315 − 0 37 1 0 CGCGAATGCCTCTGGCAGTAGATGTCAGCTCAC 1250 CTCTTTTGAGAGACGCGTGGCGGATGT NTRK3 NM_001007156 1 15 − −6 + 2 37 1 0 CGCGAATGCCTTATAGGTTTCAGAGAAATTATG 1251 TTGAATCCAATAACGCGTGGCGGATGT NTRK3 NM_001007156 1 15 − 25 − 0 37 1 0 CGCGAATGCCGGTTAAGAGGCTTGGAATGTCCG 1252 GGAAGGCTTATTACGCGTGGCGGATGT NTRK3 NM_001007156 1 15 − 60 + 0 37 1 0 CGCGAATGCCATGGCATCTATGTTGAGGATGTC 1253 AATGTTTATTTCACGCGTGGCGGATGT NTRK3 NM_001007156 1 15 − 95 − 0 37 1 0 CGCGAATGCCAAAAGGAGTTTTTAAAAGCCATG 1254 ACGTCCTTTGCTACGCGTGGCGGATGT RET NM_020630 9 10 + −14 + 0 37 1 0 CGCGAATGCCTGTGTCCTGTGCAGGGATCACCA 1255 GGAACTTCTCCAACGCGTGGCGGATGT RET NM_020630 9 10 + 21 − 0 37 1 0 CGCGAATGCCGCCGTCGGGGCAGGTCTTGGTGC 1256 TGGGAGAGCAGGACGCGTGGCGGATGT RET NM_020630 9 10 + 56 + 0 37 1 0 CGCGAATGCCCACTGCGATGTTGTGGAGACCCA 1257 AGACATCAACATACGCGTGGCGGATGT RET NM_020630 9 10 + 91 − 0 37 1 0 CGCGAATGCCTTAAACCCTGCTTACGGAGGCAG 1258 TCCTGAGGGCAAACGCGTGGCGGATGT NTRK3 NM_001012338 3 15 − −49 + 35 37 1 0 CGCGAATGCCTTTCTAAGTTTTCTTCTAATATTA 1259 TTATTGTTTTGACGCGTGGCGGATGT NTRK3 NM_001012338 3 15 − −14 − 35 37 1 0 CGCGAATGCCATTTCCAGATGGATTAAAGAGCT 1260 AAACATAAAAAAACGCGTGGCGGATGT NTRK3 NM_001012338 3 15 − 21 + 35 37 1 0 CGCGAATGCCGATTTTTGTATATGGTGTGAGGT 1261 AGGTATCTAAGCACGCGTGGCGGATGT NTRK3 NM_001012338 3 15 − 60 − 32 37 1 0 CGCGAATGCCAAAGTGTTGGGACAATGAACTAT 1262 TCATTAAAAAAAACGCGTGGCGGATGT EPHB1 NM_004441 9 3 + −38 + 0 37 1 0 CGCGAATGCCTGCCCTGTGGCTGAGAGAGCCCC 1263 TCTTTTTATCCAACGCGTGGCGGATGT EPHB1 NM_004441 9 3 + −3 − 0 37 1 0 CGCGAATGCCTGTACACAGCCTCTTTGCTATAA 1264 GCCCGTTTCCTGACGCGTGGCGGATGT EPHB1 NM_004441 9 3 + 32 + 0 37 1 0 CGCGAATGCCGCGATAAGCTCCAGCATTACAGC 1265 ACAGGCCGAGGTACGCGTGGCGGATGT EPHB1 NM_004441 9 3 + 67 − 0 37 1 0 CGCGAATGCCGTGGGGGTCAGACACCGGGTCTC 1266 TGCTTTCTACTTACGCGTGGCGGATGT PIK3CA NM_006218 12 3 + 0 + 0 0 2 0 CGCGAATGCCATGTATTGCTTGGTAAAAGATTG 1267 GCCTCCAATCAAACGCGTGGCGGATGT PIK3CA NM_006218 12 3 + 35 − 0 0 2 0 CGCGAATGCCAATTACAGTCCAGAAGTTCCATA 1268 GCCTGTTCAGGTACGCGTGGCGGATGT PIK3CA NM_006218 12 3 + 70 + 0 37 1 0 CGCGAATGCCACCCAGATCCTATGGTTCGAGGT 1269 TTTGCTGTTCGGACGCGTGGCGGATGT PIK3CA NM_006218 12 3 + 103 − 0 37 1 0 CGCGAATGCCAAGTTTGTCATCTGTTAAATATTT 1270 TTCCAAGCACCACGCGTGGCGGATGT PIK3CA NM_006218 12 3 + 136 + 5 0 2 0 CGCGAATGCCTTTCTCAGTATTTAATTCAGCTAG 1271 TACAGGTAAAAACGCGTGGCGGATGT PALB2 NM_024675 5 16 − 0 + 0 37 1 0 CGCGAATGCCGGCATTGTTTTGTTCCTCTGATGA 1272 TGAAAGTGAAAACGCGTGGCGGATGT PALB2 NM_024675 5 16 − 35 − 0 37 1 0 CGCGAATGCCAGCTTTTATATTTCCAGACTTCAG 1273 TAGTACTTGCTACGCGTGGCGGATGT PALB2 NM_024675 5 16 − 70 + 0 37 1 0 CGCGAATGCCGTGCTTGGCCTGACAAAGAGGAG 1274 GCTAGTTAGTAGACGCGTGGCGGATGT PALB2 NM_024675 5 16 − 105 − 0 37 1 0 CGCGAATGCCTGACTTCTACTTGTTGATCAGAA 1275 AGGGTCCCACTGACGCGTGGCGGATGT PALB2 NM_024675 5 16 − 140 + 0 37 1 0 CGCGAATGCCTGACGTTTGCAGAAGATGGAGGG 1276 TAAGAAAAGCATACGCGTGGCGGATGT RPS6KA1 NM_002953 18 1 + 0 + 0 37 1 0 CGCGAATGCCGTTGTGCACAGGGACCTGAAGCC 1277 CAGCAACATCCTACGCGTGGCGGATGT RPS6KA1 NM_002953 18 1 + 35 − 0 37 1 0 CGCGAATGCCGCAGGCACTCGGGATTCCCGGAC 1278 TCGTCCACATACACGCGTGGCGGATGT RPS6KA1 NM_002953 18 1 + 70 + 0 37 1 0 CGCGAATGCCGCATCTGTGACTTTGGTTTTGCCA 1279 AACAGCTGCGGACGCGTGGCGGATGT RPS6KA1 NM_002953 18 1 + 105 − 0 37 1 0 CGCGAATGCCGTGTAGCAAGGTGTCATGAGGAG 1280 CCCATTCTCAGCACGCGTGGCGGATGT RPS6KA1 NM_002953 18 1 + 140 + 0 37 1 0 CGCGAATGCCAGCCAACTTTGTGGCGCCTGAGG 1281 TGAGTGGCCCAGACGCGTGGCGGATGT PDGFRA NM_006206 17 4 + −12 + 0 37 1 0 CGCGAATGCCTCTTTTCTGCAGACTCAGAAGTC 1282 AAAAACCTCCTTACGCGTGGCGGATGT PDGFRA NM_006206 17 4 + 23 − 0 37 1 0 CGCGAATGCCTCCAATAAAGTAAGGCCTTCTGA 1283 GTTATCATCTGAACGCGTGGCGGATGT PDGFRA NM_006206 17 4 + 58 + 0 37 1 0 CGCGAATGCCTTTGTTGAGCTTCACCTATCAAGT 1284 TGCCCGAGGAAACGCGTGGCGGATGT PDGFRA NM_006206 17 4 + 93 − 0 37 1 0 CGCGAATGCCCCTTGAACTTACATTTTTTGAAGC 1285 CAAAAACTCCAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 0 + 0 37 1 0 CGCGAATGCCAACCTAAAAATAAAATATGTGTT 1286 TATGACAAGTTAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 35 − 0 37 1 0 CGCGAATGCCTCTCCAGTTTCTTCATCAAGATGG 1287 GTTTTGATGTGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 70 + 0 37 1 0 CGCGAATGCCAAAGACATCTATCACACTTGATG 1288 TTGGGCCTGAGTACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 105 − 0 37 1 0 CGCGAATGCCAGGTAATCCTCCTGGGCCATCTC 1289 CAGGGTTAAAGGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 140 + 0 37 1 0 CGCGAATGCCATACAAAGAACAGATGACACCCA 1290 AGAACATTTTCCACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 175 − 0 37 1 0 CGCGAATGCCGCTTTTGCTCACCACTAGGGTCA 1291 CTGACCCTGTGGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 210 + 0 37 1 0 CGCGAATGCCAGAAGCTGCCAAGCAGAAGAAA 1292 GAAGCAGCAGAAGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 245 − 0 37 1 0 CGCGAATGCCAAGACACAGTCTCTCTCCTGTGA 1293 AATAAATGTCCTACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 280 + 0 37 1 0 CGCGAATGCCTGGCACTGATTCACTCAGATTGT 1294 CTGGGAAAAGACACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 315 − 0 37 1 0 CGCGAATGCCAGGATTTTTGCTACTGATTTCTTC 1295 CTGTTCCTTTAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 350 + 0 37 1 0 CGCGAATGCCGCTAGATCACCAGTAACTGAAAT 1296 AAGAACTCACCTACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 385 − 0 37 1 0 CGCGAATGCCCTGGAGAATCTGGAAGTTCAGAT 1297 TTAAGACTTAAAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 420 + 0 37 1 0 CGCGAATGCCAACCAGTTACAGAAATTAATGAA 1298 GACAGTGTATTAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 455 − 0 37 1 0 CGCGAATGCCTCAACACCTTTTTCTGGTTGGGCA 1299 GTTGGTGGAATACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 490 + 0 37 1 0 CGCGAATGCCTACATTCCTAAGAAGACCTAATT 1300 TCACCAGGGCGAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 525 − 0 37 1 0 CGCGAATGCCACCGCTATCTGATAGAGTCTGTA 1301 AAGGAACTGTAGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 559 + 0 37 1 0 CGCGAATGCCTAGTAGTCAGCACCTTGAACACA 1302 TTCCTCCTAAAGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 595 − 0 37 1 0 CGCGAATGCCTGTTTTTTAGGTCGTGAGTAGTAA 1303 GTTCACTGCTAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 630 + 0 37 1 0 CGCGAATGCCTTAGATTTACTTCACCTGTAAGTT 1304 TGGAGGCACAAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 665 − 0 37 1 0 CGCGAATGCCAGGAGGTTATCTGTAGAGACAGT 1305 CATTTTTTTGCCACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 700 + 0 37 1 0 CGCGAATGCCTGTAAATAAAGCTATAAGTAAAA 1306 GTGGCCAACTGCACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 735 − 0 37 1 0 CGCGAATGCCACATGAAATATTTGCCTCTAAAT 1307 TAGAACTTGTGGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 770 + 0 37 1 0 CGCGAATGCCTCTCTAAATGAACTCACCTACAA 1308 TAACTTACCAGCACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 805 − 0 37 1 0 CGCGAATGCCTTTGATTTTGTTCTTTTAAGTTTTG 1309 GTTTTCATTTACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 840 + 0 37 1 0 CGCGAATGCCCAGAGAAATCTTTAAAATCTCCC 1310 AGTGACACTCTTACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 875 − 0 37 1 0 CGCGAATGCCATCTCACTTTCCTGAAGATTTTCA 1311 TTCCTGCCATCACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 910 + 0 37 1 0 CGCGAATGCCTCTAAGTCAACCTAAGAGTCTTA 1312 GCCTGGAAGCAAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 945 − 0 37 1 0 CGCGAATGCCTGTGCAAGAATGTTTTTCTGCAG 1313 AAAGAGGAGAGGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 980 + 0 37 1 0 CGCGAATGCCGTGCCTGAAGGCCTTCTGTTTCCT 1314 GCAGAATATTAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1015 − 0 37 1 0 CGCGAATGCCTCTGGCAATTGGACATGCTTCGT 1315 GTTGTTCTAACAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1050 + 0 37 1 0 CGCGAATGCCGGAAAGTAGCCGTGGAGGCTGTC 1316 ATTCAGAGTCATACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1085 − 0 37 1 0 CGCGAATGCCTTTTTATTTTTAAACCCTTTTTTCT 1317 TGACATCCAAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1120 + 0 37 1 0 CGCGAATGCCTAAGGATGCAAGTAAAAATTTAA 1318 ACCTTTCCAATGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1155 − 0 37 1 0 CGCGAATGCCGCCAGACATCCTAATTTCACTTTG 1319 GTCAGTTTCCTACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1190 + 0 37 1 0 CGCGAATGCCACATGCACAGGACAACCAAGTTC 1320 AAGAACCTCTCAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1225 − 0 37 1 0 CGCGAATGCCCGGGAGAGCTGACTTTAGTTAAT 1321 GAGAGAAGTTTCACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1260 + 0 37 1 0 CGCGAATGCCCTGGGCCCACTGAAGATAATGAC 1322 TTGTCTAGGAAGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1295 − 0 37 1 0 CGCGAATGCCCCTGTGTATCTTCTACCAGGTGCT 1323 TGGGCAACTGCACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1330 + 0 37 1 0 CGCGAATGCCAAAAAGAAAATCAGCCTGCACCC 1324 CAGCATCAGATCACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1365 − 0 37 1 0 CGCGAATGCCCGACAGGCTAGAAGTTGGCAAAA 1325 GTGGTTCACAATACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1400 + 0 37 1 0 CGCGAATGCCATTGTTAACAGGTCCAAGGAAGA 1326 AGTCACCTCACAACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1435 − 0 37 1 0 CGCGAATGCCTCACTTGAATAAATAATTTTTCGT 1327 GCTGATATTTGACGCGTGGCGGATGT PALB2 NM_024675 10 16 − 1466 + 11 37 1 0 CGCGAATGCCGTGAAAGGTAAATCAAGATGTGT 1328 TTGATGATGATGACGCGTGGCGGATGT RB1 NM_000321 8 13 + 0 + 0 37 1 0 CGCGAATGCCAAACAGCTGTTATACCCATTAAT 1329 GGTTCACCTCGAACGCGTGGCGGATGT RB1 NM_000321 8 13 + 35 − 0 37 1 0 CGCGAATGCCATCCGTGCACTCCTGTTCTGACCT 1330 CGCCTGGGTGTACGCGTGGCGGATGT RB1 NM_000321 8 13 + 70 + 0 37 1 0 CGCGAATGCCAGCAAAACAACTAGAAAATGAT 1331 ACAAGAATTATTGACGCGTGGCGGATGT RB1 NM_000321 8 13 + 105 − 0 37 1 0 CGCGAATGCCATCTATATTACATTCATGTTCTTT 1332 ACAGAGAACTTACGCGTGGCGGATGT RB1 NM_000321 8 13 + 140 + 0 37 1 0 CGCGAATGCCGAGGTAATTTAACTTCATGATTTC 1333 TTTAAAACAGTACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 0 + 0 37 1 0 CGCGAATGCCCAACTCCATGGGAAGAACCTGGT 1334 TTTTAGTGACGGACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 35 − 0 37 1 0 CGCGAATGCCAGGAGCCCACACCAATTGTCTCC 1335 TTTACCACGTAGACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 70 + 0 37 1 0 CGCGAATGCCACTCTGAGTGCAAGCGCTGTGTC 1336 CACAAGGCCACCACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 105 − 0 37 1 0 CGCGAATGCCGTCAGGAGGCCCACCTTGACAGC 1337 ATACTCCATGTTACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 140 + 0 37 1 0 CGCGAATGCCCACGTCTCGGCCAAGGCTGCTGG 1338 GTTGGGGGCAGGACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 175 − 0 37 1 0 CGCGAATGCCGAGCTCAGGCACCATCCCTCCCC 1339 ACCAGACGGGGAACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 210 + 0 37 1 0 CGCGAATGCCTGCAGATGTATGAAAGGTGTGTG 1340 GCCGAGACCTCCACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 245 − 0 37 1 0 CGCGAATGCCGGGTCCAGGGTCCTTTCTGGCCA 1341 TGGAGCAGGCCAACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 280 + 0 37 1 0 CGCGAATGCCTGTCACCCTGACACTGCCACATG 1342 CACCCCCTTTCTACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 315 − 0 37 1 0 CGCGAATGCCCTGAAGGATCCCGCTTGCTCTTAT 1343 CAATGACCTGAACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 350 + 0 37 1 0 CGCGAATGCCAAGAGATTGAGATTCTTCTGCGG 1344 TATGGCCAGCACACGCGTGGCGGATGT RPS6KA1 NM_002953 16 1 + 385 − 0 37 1 0 CGCGAATGCCCCCCCACTCACATCTTTCAGAGT 1345 GATGATGTTGGGACGCGTGGCGGATGT EPHB1 NM_004441 1 3 + −41 + 0 37 1 0 CGCGAATGCCCTGCCTCGGCTTGGTCTCGGCCTG 1346 CGGGCCGTCGGACGCGTGGCGGATGT EPHB1 NM_004441 1 3 + −6 − 0 37 1 0 CGCGAATGCCAGGAGCAGTAGTAGATAATCCAG 1347 GGCCATCGCCGGACGCGTGGCGGATGT EPHB1 NM_004441 1 3 + 29 + 0 37 1 0 CGCGAATGCCCCTGGCATCCGCAGTGGCTGCGA 1348 TGGAAGGTAACGACGCGTGGCGGATGT EPHB1 NM_004441 1 3 + 64 − 0 37 1 0 CGCGAATGCCCCGGCACCAGCAGCCAACTTGCT 1349 CCGTGGAGGGTAACGCGTGGCGGATGT PALB2 NM_024675 1 16 − 0 + 0 37 1 0 CGCGAATGCCGTTCCTGGAAGGTGACGTGAAAG 1350 ATCACTGTGCAGACGCGTGGCGGATGT PALB2 NM_024675 1 16 − 35 − 0 37 1 0 CGCGAATGCCCCAAATGGCAATTGTTCCAGAAG 1351 TCAAGATTGCTGACGCGTGGCGGATGT PALB2 NM_024675 1 16 − 70 + 0 37 1 0 CGCGAATGCCGACTTACTTCTCGGTCAGTGTACT 1352 GCCCTCCTCCCACGCGTGGCGGATGT PALB2 NM_024675 1 16 − 105 − 0 37 1 0 CGCGAATGCCATTTCACAAAAGACCAATGTTGG 1353 TCAGAGACAGGTACGCGTGGCGGATGT PALB2 NM_024675 1 16 − 140 + 0 37 1 0 CGCGAATGCCGGTCGGGTACAGACTCTCATTTG 1354 CTGGCTGGACAAACGCGTGGCGGATGT PALB2 NM_024675 1 16 − 175 − 0 37 1 0 CGCGAATGCCTATGAATAGTGGTATACAAATAT 1355 ATTTCCATCTTTACGCGTGGCGGATGT PALB2 NM_024675 1 16 − 210 + 0 37 1 0 CGCGAATGCCAGTTAGGGTAAAGTGAAAACACA 1356 ATTTTCTGGATAACGCGTGGCGGATGT CENTG1 NM_014770 6 12 − −6 + 0 37 1 0 CGCGAATGCCCCTAAGACTGTACCCCATCTGGA 1357 GACCTGAGCCCCACGCGTGGCGGATGT CENTG1 NM_014770 6 12 − 29 − 0 37 1 0 CGCGAATGCCTTCACCATGGGAGAAGGAGGGG 1358 GTTCCCGACTCAGACGCGTGGCGGATGT CENTG1 NM_014770 6 12 − 64 + 0 37 1 0 CGCGAATGCCGAAGCAGAGGAGGAAAAAATTG 1359 ACAACACCATCCAACGCGTGGCGGATGT CENTG1 NM_014770 6 12 − 99 − 0 37 1 0 CGCGAATGCCCTCACCTTCAGCCTGCCCAGCCG 1360 AGCCTTCAGTCTACGCGTGGCGGATGT EPHA7 NM_004440 11 6 − 0 + 0 37 1 0 CGCGAATGCCGATCAAAGGGAACGGACCTACTC 1361 AACAGTAAAAACACGCGTGGCGGATGT EPHA7 NM_004440 11 6 − 35 − 0 37 1 0 CGCGAATGCCGTTTCAGATTATTAATGGAGGCT 1362 GAAGTAGACTTGACGCGTGGCGGATGT EPHA7 NM_004440 11 6 − 70 + 0 37 1 0 CGCGAATGCCCAGGAACAGTGTATGTTTTCCAG 1363 ATTCGGGCTTTTACGCGTGGCGGATGT EPHA7 NM_004440 11 6 − 105 − 0 37 1 0 CGCGAATGCCAGTCTGGGACTGTAATTTCCATA 1364 ACCAGCAGCAGTACGCGTGGCGGATGT EPHA7 NM_004440 11 6 − 140 + 0 37 1 0 CGCGAATGCCTGATGTTGCTACACTAGAGGAAG 1365 CTACAGGTAAAAACGCGTGGCGGATGT EPHA7 NM_004440 11 6 − 175 − 0 37 1 0 CGCGAATGCCGGAATCCAAACCAAAGGCATAAT 1366 TACCTTCAAACAACGCGTGGCGGATGT RB1 NM_000321 5 13 + −50 + 0 37 1 0 CGCGAATGCCCTTCTAAATTACGAAAAAATGTT 1367 AAAAAGTCATAAACGCGTGGCGGATGT RB1 NM_000321 5 13 + −15 − 0 37 1 0 CGCGAATGCCAATATATAAGTTCACATGTCCTG 1368 AAAAGAAAAACAACGCGTGGCGGATGT RB1 NM_000321 5 13 + 20 + 0 37 1 0 CGCGAATGCCTGACACAACCCAGCAGTTCGTAA 1369 GTAGTTCACAGAACGCGTGGCGGATGT RB1 NM_000321 5 13 + 55 − 0 37 1 0 CGCGAATGCCCATAAAAATCTTTTTTTTTAAGTG 1370 AAAAATAACATACGCGTGGCGGATGT EPHA4 NM_004438 4 2 − 0 + 0 37 1 0 CGCGAATGCCGTGATTAAAGCCATTGAGGAAGG 1371 CTATCGGTTACCACGCGTGGCGGATGT EPHA4 NM_004438 4 2 − 35 − 0 37 1 0 CGCGAATGCCGCTGGTGGAGCGCAATGGGGCAG 1372 TCCATTGGAGGGACGCGTGGCGGATGT EPHA4 NM_004438 4 2 − 70 + 0 37 1 0 CGCGAATGCCTGATGCTAGACTGCTGGCAGAAG 1373 GAGAGGAGCGACACGCGTGGCGGATGT EPHA4 NM_004438 4 2 − 105 − 0 37 1 0 CGCGAATGCCTCCAACATGTTGACAATCTGCCC 1374 AAATTTAGGCCTACGCGTGGCGGATGT EPHA4 NM_004438 4 2 − 140 + 0 37 1 0 CGCGAATGCCCAAACTCATCCGCAACCCCAACA 1375 GCTTGAAGAGGAACGCGTGGCGGATGT EPHA4 NM_004438 4 2 − 175 − 0 37 1 0 CGCGAATGCCAGTAAGCATGGCTGACCTGGAGC 1376 TCTCCGTCCCTGACGCGTGGCGGATGT EPHA7 NM_004440 1 6 − −12 + 0 37 1 0 CGCGAATGCCTGTCTTTTTCAGGGATGTGATGA 1377 GTTTAGGGATCAACGCGTGGCGGATGT EPHA7 NM_004440 1 6 − 23 − 0 37 1 0 CGCGAATGCCGCTGCTCATGATTTTCTTTTGATG 1378 ACCAACCAGTGACGCGTGGCGGATGT EPHA7 NM_004440 1 6 − 58 + 0 37 1 0 CGCGAATGCCATTCAGACTATGAGAGCACAAAT 1379 GCTACATTTACAACGCGTGGCGGATGT EPHA7 NM_004440 1 6 − 93 − 0 37 1 0 CGCGAATGCCGGAGAAATGCATATCACACTTGA 1380 ATGCCAGTTCCAACGCGTGGCGGATGT RPS6KA1 NM_002953 11 1 + −26 + 0 37 1 0 CGCGAATGCCTTGATGAGTCCCGGGGGCTGTTT 1381 CAGGGCGAAGCTACGCGTGGCGGATGT RPS6KA1 NM_002953 11 1 + 9 − 0 37 1 0 CGCGAATGCCTCTGGGCTTCAGTGCTCAGAAAC 1382 TGGGGCATGCCTACGCGTGGCGGATGT RPS6KA1 NM_002953 11 1 + 44 + 0 37 1 0 CGCGAATGCCGCCTCTTGCGGGCCCTGTTCAAG 1383 CGGAATCCTGCCACGCGTGGCGGATGT RPS6KA1 NM_002953 11 1 + 79 − 0 37 1 0 CGCGAATGCCCCTCCCCTGAGCTGGGGCTGCTT 1384 ACCGAGCCGGTTACGCGTGGCGGATGT EPHB1 NM_004441 2 3 + −38 + 0 37 1 0 CGCGAATGCCTTGTTTTTGTTTATTCGTTTTTTCT 1385 TTTTAATCTAACGCGTGGCGGATGT EPHB1 NM_004441 2 3 + −3 − 0 37 1 0 CGCGAATGCCTGCAGTAGCCGTTCTGGTGTCCA 1386 TTAACGTTTCTGACGCGTGGCGGATGT EPHB1 NM_004441 2 3 + 32 + 0 37 1 0 CGCGAATGCCGAGCTGGGCTGGACGGCCAATCC 1387 TGCGTCCGGGGTACGCGTGGCGGATGT EPHB1 NM_004441 2 3 + 67 − 0 37 1 0 CGCGAATGCCCATAGCAGGACTGAAAGACGAAT 1388 GGTTTGATACTCACGCGTGGCGGATGT PDGFRA NM_006206 19 4 + −14 + 0 37 1 0 CGCGAATGCCCTCCTTCCTTGCAGACCTTTCTGC 1389 CCGTGAAGTGGACGCGTGGCGGATGT PDGFRA NM_006206 19 4 + 21 − 0 37 1 0 CGCGAATGCCGTGTAGAGGTTGTCAAAGATGCT 1390 CTCAGGAGCCATACGCGTGGCGGATGT PDGFRA NM_006206 19 4 + 56 + 0 37 1 0 CGCGAATGCCCACACTGAGTGATGTCTGGTCTT 1391 ATGGCATTCTGCACGCGTGGCGGATGT PDGFRA NM_006206 19 4 + 91 − 0 37 1 0 CGCGAATGCCTGTCAGGCCCATACCAAGGGAAA 1392 AGATCTCCCAGAACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 0 + 0 37 1 0 CGCGAATGCCAGGAAAACTTTGAGTTCCTGATC 1393 GTGTCCAGCACGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 35 − 0 37 1 0 CGCGAATGCCTCAAAACTGGCTGCCTCAAAGTG 1394 CCACGTCTGACCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 70 + 0 37 1 0 CGCGAATGCCGGAGCGGGATGCCTGGGTCCAGG 1395 CCATCGAGAGTCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 105 − 0 37 1 0 CGCGAATGCCGCTGCTCTCACAGCATTGCAGAC 1396 TGGCTAGGATCTACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 140 + 0 37 1 0 CGCGAATGCCAAGGTCAAGGTAAGAGTTTGAGG 1397 TGGAGTGGAGGAACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 175 − 0 37 1 0 CGCGAATGCCTGGCCGTCAGCACCAGGGTCAGT 1398 TCCTGCGGCCAGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 210 + 0 37 1 0 CGCGAATGCCTTGGCAACGACACGGCCGAAGTC 1399 GTGTGAGAGGAGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 245 − 0 37 1 0 CGCGAATGCCCTCCAAGCCCCACCTCCTTCTCAG 1400 ACACCTCTGCTACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 280 + 0 37 1 0 CGCGAATGCCGCAGAGTCAGTGCCAACCCAAAC 1401 CCTCTCTGCAGCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 315 − 0 37 1 0 CGCGAATGCCGATGGCCACGGCCTCGCTTTGGC 1402 TGTCTGTGCGCAACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 350 + 0 37 1 0 CGCGAATGCCCAGGCGATCCGGAACGCCAAGG 1403 GGAATTCAATCTGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 385 − 0 37 1 0 CGCGAATGCCCCTCCTGGCACTCACTGGGGGCC 1404 CCGCAGTCCACGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 420 + 0 37 1 0 CGCGAATGCCCAGCGGAGGGGCTAGGGAGTGT 1405 AGTGAATGCCGGGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 455 − 0 37 1 0 CGCGAATGCCGTAGAGGTCGGCTCCCAGCCGGG 1406 CAGCACAGGCACACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 490 + 0 37 1 0 CGCGAATGCCGCTCCTTCCGCAGACCCCACGTG 1407 GGCCAGCTTGAAACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 525 − 0 37 1 0 CGCGAATGCCTGCCAGAACACTCGATGCAGATG 1408 AGGGCGCCCAGGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 560 + 0 37 1 0 CGCGAATGCCTCCACCGCAACCTGGGCACACAC 1409 CTGTCCCGCGTTACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 595 − 0 37 1 0 CGCGAATGCCAGCTCCCGTGGCCAGTCGTCCAA 1410 GTCCAGCGAGCGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 633 + 0 37 1 0 CGCGAATGCCCCTGGTGCTGACGGCTATTGGCA 1411 ACGACACGGCCAACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 665 − 0 37 1 0 CGCGAATGCCACGGCCTCGCGTGTCGCTTTCCC 1412 ACACGCGGTTGGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 700 + 0 37 1 0 CGCGAATGCCGCCAAGCCCTCGCGGGACTCTTC 1413 GCGGTAAGCGTGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 735 − 0 37 1 0 CGCGAATGCCACACCCTCAGCAACCCTCCCCCC 1414 GCTCTGTTCCCTACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 770 + 0 37 1 0 CGCGAATGCCGCGAACCTGAGACGGTCCCGTGG 1415 GTAGGGGCAGAAACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 805 − 0 37 1 0 CGCGAATGCCCAACGGAAAAGGCTCTAGGGACC 1416 CCCAGCCAGGACACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 840 + 0 37 1 0 CGCGAATGCCATCCCTGGTCTTGCAGGGAGGAG 1417 CGCGAGTCGTGGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 875 − 0 37 1 0 CGCGAATGCCGCCAGGAACAGTAGCTGCTCGTA 1418 CTTGGCGCGAATACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 910 + 0 37 1 0 CGCGAATGCCGCCGCTGAGCACCTCGGAGGAGC 1419 CGCTGGGCCGCCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 945 − 0 37 1 0 CGCGAATGCCAGCCACGTCCTGGGCCTGCACGG 1420 CGGCCCACAGCTACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 980 + 0 37 1 0 CGCGAATGCCACCGTTCTCCTGCTTTTGGCCCAT 1421 GCGCGACACGGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1015 − 0 37 1 0 CGCGAATGCCGCAGCTGTGGGTCCTCTACGCTG 1422 GTGTCGAGCGGCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1050 + 0 37 1 0 CGCGAATGCCGCTCCCCACTCCACCTGGCGGCC 1423 GAGCTCGCCCACACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1085 − 0 37 1 0 CGCGAATGCCTCACGTACCCACAGCAGCAGTTG 1424 CGTGATGACGACACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1120 + 0 37 1 0 CGCGAATGCCGCTGGGGGGAGGAAAGGGGGTC 1425 TTTGAGGCTTCATACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1155 − 0 37 1 0 CGCGAATGCCAATTTCGGGCTTTCCCGCGCCAG 1426 GCGTTTTCCGAGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1190 + 0 37 1 0 CGCGAATGCCCCCAGAGGGACCCCGGAAGTAG 1427 GCTTGGCCATGTGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1225 − 0 37 1 0 CGCGAATGCCGTCCCCAGGGCGCCCACACCCGG 1428 CGCCGCCTCCCCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1260 + 0 37 1 0 CGCGAATGCCGCGCGGTCACGCGGCCGTTTCCG 1429 CCCTCTAGTACGACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1295 − 0 37 1 0 CGCGAATGCCGCGGCCCTGGGCGTCACGGGCCG 1430 CCACGTCCGCGCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1330 + 0 37 1 0 CGCGAATGCCACGGCGCTGTTCTACGCCCGCCA 1431 GGCTGGAAGCCAACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1365 − 0 37 1 0 CGCGAATGCCGGCAGCCGTGCTGGAGAAGGATG 1432 TCGGCGCACAGCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1400 + 0 37 1 0 CGCGAATGCCCGGGTGAGGGCGGCAGCGCGGC 1433 CACCACGCCCAGCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1435 − 0 37 1 0 CGCGAATGCCCTGGGCGTGGCGGTGATGCTGGG 1434 CGTGGTGGCCGCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1470 + 0 37 1 0 CGCGAATGCCCCCCCGCCGCCGGAGCAGCGCCG 1435 CTAGCGTGGGCCACGCGTGGCGGATGT CENTG1 NM_014770 5 12 − 1505 − 0 37 1 0 CGCGAATGCCGGGCAACTATACCAGCGCAACCG 1436 GGGCGTCGGCGCACGCGTGGCGGATGT EPHA7 NM_004440 13 6 − 0 + 0 37 1 0 CGCGAATGCCGGCCTCCATCTGCACCACAGAAC 1437 CTCATTTTCAACACGCGTGGCGGATGT EPHA7 NM_004440 13 6 − 35 − 0 37 1 0 CGCGAATGCCGGACTCCATTCCAAACTTACTGT 1438 GGTTTGGTTGATACGCGTGGCGGATGT EPHA7 NM_004440 13 6 − 70 + 0 37 1 0 CGCGAATGCCTCCTGCAGACAATGGGGGAAGAA 1439 ACGATGTGACCTACGCGTGGCGGATGT EPHA7 NM_004440 13 6 − 105 − 0 37 1 0 CGCGAATGCCCTGCTCCCAACTGCACCGCTTAC 1440 ACAATATTCTGTACGCGTGGCGGATGT EPHA7 NM_004440 13 6 − 140 + 0 37 1 0 CGCGAATGCCGGCGAATGTGTTCCCTGTGGGAG 1441 TAACATTGGATAACGCGTGGCGGATGT EPHA7 NM_004440 13 6 − 175 − 0 37 1 0 CGCGAATGCCCATAGTTATCCTCTAATCCAGTCT 1442 GCTGGGGCATGACGCGTGGCGGATGT EPHA7 NM_004440 13 6 − 210 + 0 37 1 0 CGCGAATGCCTCACTGTCATGGACCTGCTAGCC 1443 CACGCTAATTATACGCGTGGCGGATGT EPHA7 NM_004440 13 6 − 245 − 0 37 1 0 CGCGAATGCCTCAGAAACTCCATTTACAGCTTC 1444 AACTTCAAAAGTACGCGTGGCGGATGT EPHA7 NM_004440 13 6 − 280 + 0 37 1 0 CGCGAATGCCCTTAAGCCGATCCCAGAGGCTCT 1445 TTGCTGCTGTCAACGCGTGGCGGATGT EPHA7 NM_004440 13 6 − 315 − 0 37 1 0 CGCGAATGCCAACACAAAACATACCTGCTTGAC 1446 CAGTGGTGATACACGCGTGGCGGATGT CHAF1A NM_005483 7 19 + −36 + 0 37 1 0 CGCGAATGCCTCTCCTCTTTCTCATCACCATCTC 1447 TTAACATCACAACGCGTGGCGGATGT CHAF1A NM_005483 7 19 + −1 − 0 37 1 0 CGCGAATGCCACCTCGTGATTTCGGCCTTCTCTG 1448 CTTTAATGCGCACGCGTGGCGGATGT CHAF1A NM_005483 7 19 + 34 + 0 37 1 0 CGCGAATGCCTCTTCCAGAAACCAAAGACTCCA 1449 CAGGCCCCCAAGACGCGTGGCGGATGT CHAF1A NM_005483 7 19 + 69 − 0 37 1 0 CGCGAATGCCCTGAAACCCAAAAGCAAAGGCA 1450 GCCGGCTGCTCACACGCGTGGCGGATGT PKN1 NM_002741 6 19 + 0 + 0 37 1 0 CGCGAATGCCGCCCAGGAGAAATTAACAGAATC 1451 CAACCAGAAGCTACGCGTGGCGGATGT PKN1 NM_002741 6 19 + 35 − 0 37 1 0 CGCGAATGCCCAAGTCTCCGCTCCAGAGCCTCC 1452 CGCAGCAGCCCCACGCGTGGCGGATGT PKN1 NM_002741 6 19 + 70 + 0 37 1 0 CGCGAATGCCGGGAGCTGCCCGCCGACCACCCC 1453 AAGGGGCGGCTGACGCGTGGCGGATGT PKN1 NM_002741 6 19 + 105 − 0 37 1 0 CGCGAATGCCGCAGCGGAGGAGGCCGCAGCGA 1454 GCTCTTCTCGCAGACGCGTGGCGGATGT PKN1 NM_002741 6 19 + 140 + 0 37 1 0 CGCGAATGCCCTTCAGCACCCGCCTGGCCGGGC 1455 CCTTTCCCGCCAACGCGTGGCGGATGT PKN1 NM_002741 6 19 + 175 − 0 37 1 0 CGCGAATGCCGAGCGGCGCGGGCTTGCACAGGG 1456 TGCTGTAGTGCGACGCGTGGCGGATGT PKN1 NM_002741 6 19 + 210 + 0 37 1 0 CGCGAATGCCACAGGTGGGTCTGAGACCCTACC 1457 CCACCCCTGCAGACGCGTGGCGGATGT EPHA3 NM_005233 2 3 + −38 + 0 37 1 0 CGCGAATGCCTGTTATTAACTGTGTTTGTGTATT 1458 ATGTTTTATTTACGCGTGGCGGATGT EPHA3 NM_005233 2 3 + −3 − 0 37 1 0 CGCGAATGCCCCCTTGAATTGTTTTTGAATCCAG 1459 TAGATTGACTAACGCGTGGCGGATGT EPHA3 NM_005233 2 3 + 32 + 0 37 1 0 CGCGAATGCCGAGCTGGGCTGGATCTCTTATCC 1460 ATCACATGGGGTACGCGTGGCGGATGT EPHA3 NM_005233 2 3 + 67 − 0 37 1 0 CGCGAATGCCGAGAAAATGTTTCCTTGTGATAG 1461 TTTATTGAACTCACGCGTGGCGGATGT KSR2 NM_173598 5 12 − −3 + 0 37 1 0 CGCGAATGCCCAGGGCATGGGCTACCTCCACGC 1462 CAAGGGAATCCTACGCGTGGCGGATGT KSR2 NM_173598 5 12 − 32 − 0 37 1 0 CGCGAATGCCCATAGAAGACGTTCTTTGACTTG 1463 AGGTCCTTGTGTACGCGTGGCGGATGT KSR2 NM_173598 5 12 − 67 + 0 37 1 0 CGCGAATGCCACAACGGCAAAGTGGTCATCACG 1464 GACTTTGGACTCACGCGTGGCGGATGT KSR2 NM_173598 5 12 − 102 − 0 37 1 0 CGCGAATGCCCACCTGCCAGCCTGCAGCACCCC 1465 AGAAATGCTGAAACGCGTGGCGGATGT RPS6KA1 NM_002953 1 1 + −38 + 0 37 1 0 CGCGAATGCCGGCCGCCGGAGGAGCGCGGGTG 1466 ACCTGGCGGCGGCACGCGTGGCGGATGT RPS6KA1 NM_002953 1 1 + −3 − 0 37 1 0 CGCGAATGCCGGCCAGGGCTCCTTGAGCTGGGC 1467 GAGCGGCATCTCACGCGTGGCGGATGT RPS6KA1 NM_002953 1 1 + 32 + 0 37 1 0 CGCGAATGCCGCTCATGGAGCTAGTGCCTCTGG 1468 ACCCGGAGGTGAACGCGTGGCGGATGT RPS6KA1 NM_002953 1 1 + 63 − 27 37 1 0 CGCGAATGCCGCCGCGGGCGCCCGTCCCCCGCC 1469 CCGCTCACTCACACGCGTGGCGGATGT KSR2 NM_173598 18 12 − 0 + 0 37 1 0 CGCGAATGCCGAAATCTCCCCCGGCCAGCTGAG 1470 CCTGGAGGACCTACGCGTGGCGGATGT KSR2 NM_173598 18 12 − 35 − 0 37 1 0 CGCGAATGCCCAGTCTCGCACACCTGTTCATCC 1471 GTCATCTCCAAGACGCGTGGCGGATGT KSR2 NM_173598 18 12 − 70 + 0 37 1 0 CGCGAATGCCTGGAGAAATACGGAGCCAACCG 1472 GGAGGAGTGTGCCACGCGTGGCGGATGT KSR2 NM_173598 18 12 − 105 − 0 37 1 0 CGCGAATGCCACATTCCTGAGGCAGGAGAGGGA 1473 GGCGTTGAGGCGACGCGTGGCGGATGT KSR2 NM_173598 18 12 − 140 + 0 37 1 0 CGCGAATGCCCCACATGTCAGGTGAGCAGGCCC 1474 CGGGGTCGGGGAACGCGTGGCGGATGT GUCY2F NM_001522 10 X − 0 + 0 37 1 0 CGCGAATGCCAGCTGCTGTGGACGGCCCCTGAA 1475 CTGTTGAGAGCTACGCGTGGCGGATGT GUCY2F NM_001522 10 X − 35 − 0 37 1 0 CGCGAATGCCTCTCCTGCAAAAGAACCTAACCT 1476 GCTGCCTCTTGGACGCGTGGCGGATGT GUCY2F NM_001522 10 X − 70 + 0 37 1 0 CGCGAATGCCTGTCTATAGCTTTGCCATCATCAT 1477 GCAAGAAGTGAACGCGTGGCGGATGT GUCY2F NM_001522 10 X − 105 − 0 37 1 0 CGCGAATGCCCAGATCCATCATGCAGAATGGGG 1478 TACCCCGGACCAACGCGTGGCGGATGT GUCY2F NM_001522 10 X − 140 + 0 37 1 0 CGCGAATGCCCCAGCTCAAGGTAAGCGGGAGGT 1479 GAGAAAAGGGCCACGCGTGGCGGATGT PKN1 NM_002741 2 19 + 0 + 0 37 1 0 CGCGAATGCCAGTGAGCCTCGCAGCTGGTCCCT 1480 GCTAGAGCAGCTACGCGTGGCGGATGT PKN1 NM_002741 2 19 + 35 − 0 37 1 0 CGCGAATGCCCCCCGGGGGCCGCCAGGTCTGCC 1481 CCGGCCAGGCCCACGCGTGGCGGATGT PKN1 NM_002741 2 19 + 70 + 0 37 1 0 CGCGAATGCCTACAGCAGCAGCTGGAGCTGGAG 1482 CGGGAGCGGCTGACGCGTGGCGGATGT PKN1 NM_002741 2 19 + 105 − 0 37 1 0 CGCGAATGCCTCCTTCAGCTTCAGCTCCTTGCGG 1483 ATTTCCCGCCGACGCGTGGCGGATGT PKN1 NM_002741 2 19 + 140 + 0 37 1 0 CGCGAATGCCGGGTGCTGAGAACCTGCGGCGGG 1484 CCACCACTGACCACGCGTGGCGGATGT PKN1 NM_002741 2 19 + 175 − 0 37 1 0 CGCGAATGCCCAGCAGCAGCTCTACGGGGCCCA 1485 GGCTGCGGCCCAACGCGTGGCGGATGT PKN1 NM_002741 2 19 + 210 + 0 37 1 0 CGCGAATGCCCGGGGCTCCTCGCGCCGCCTCGA 1486 CCTGCTGCACCAACGCGTGGCGGATGT PKN1 NM_002741 2 19 + 245 − 0 37 1 0 CGCGAATGCCGAAGCACCACGTGGGCGTGCAGC 1487 TCCTGCAGCTGCACGCGTGGCGGATGT PKN1 NM_002741 2 19 + 280 + 0 37 1 0 CGCGAATGCCCCGACCCGGCGGCCACCCACGGT 1488 GAGCTGGGATGCACGCGTGGCGGATGT RBBP8 NM_002894 7 18 + 0 + 0 37 1 0 CGCGAATGCCGAATGATCAACAGCATCAAGCAG 1489 CTGAGCTTGAATACGCGTGGCGGATGT RBBP8 NM_002894 7 18 + 35 − 0 37 1 0 CGCGAATGCCTGTTATCGGTGAATCTGGAATAA 1490 CGTCTTCCTCACACGCGTGGCGGATGT RBBP8 NM_002894 7 18 + 70 + 0 37 1 0 CGCGAATGCCGCCTTCTCATTTTCTGGCGTTAAC 1491 CGGCTACGAAGACGCGTGGCGGATGT RBBP8 NM_002894 7 18 + 105 − 0 37 1 0 CGCGAATGCCTTTGTTCTATGTATCGGACATGGG 1492 GGTTCTCCTTTACGCGTGGCGGATGT RBBP8 NM_002894 7 18 + 140 + 0 37 1 0 CGCGAATGCCCACATACTAAATTGGAGCACTCT 1493 GTGTGTGCAAATACGCGTGGCGGATGT RBBP8 NM_002894 7 18 + 175 − 0 37 1 0 CGCGAATGCCAACCAGAGAACTAAAATACAACT 1494 CCAACTCTTACCACGCGTGGCGGATGT EPHB1 NM_004441 13 3 + 0 + 0 37 1 0 CGCGAATGCCGGAGGGAAGATCCCTGTGAGATG 1495 GACAGCTCCAGAACGCGTGGCGGATGT EPHB1 NM_004441 13 3 + 35 − 0 37 1 0 CGCGAATGCCCGCTGGCTGAAGTGAACTTGCGG 1496 TAGGCGATGGCCACGCGTGGCGGATGT EPHB1 NM_004441 13 3 + 70 + 0 37 1 0 CGCGAATGCCACGTTTGGAGCTATGGGATCGTC 1497 ATGTGGGAAGTCACGCGTGGCGGATGT EPHB1 NM_004441 13 3 + 105 − 0 37 1 0 CGCGAATGCCGACATATCCCAATAGGGTCTCTC 1498 TCCAAATGACATACGCGTGGCGGATGT EPHB1 NM_004441 13 3 + 140 + 0 37 1 0 CGCGAATGCCCAACCAAGATGTGAGTGTCAGCA 1499 GCACTTGGTCACACGCGTGGCGGATGT PDGFRA NM_006206 12 4 + −4 + 0 37 1 0 CGCGAATGCCATAGAAACCGAGGTATGAAATTC 1500 GCTGGAGGGTCAACGCGTGGCGGATGT PDGFRA NM_006206 12 4 + 31 − 0 37 1 0 CGCGAATGCCAATATATTCATGTCCATCTGGGCT 1501 GATTGATTCAAACGCGTGGCGGATGT PDGFRA NM_006206 12 4 + 66 + 0 37 1 0 CGCGAATGCCTATGTGGACCCGATGCAGCTGCC 1502 TTATGACTCAAGACGCGTGGCGGATGT PDGFRA NM_006206 12 4 + 101 − 0 37 1 0 CGCGAATGCCTACCAAGCACTAGTCCATCTCTT 1503 GGAAACTCCCATACGCGTGGCGGATGT RET NM_020630 7 10 + 0 + 0 37 1 0 CGCGAATGCCATCGGGAAAGTCTGTGTGGAAAA 1504 CTGCCAGGCATTACGCGTGGCGGATGT RET NM_020630 7 10 + 35 − 0 37 1 0 CGCGAATGCCAGGAATGCAGCTTGTACTGGACG 1505 TTGATGCCACTGACGCGTGGCGGATGT RET NM_020630 7 10 + 70 + 0 37 1 0 CGCGAATGCCCTGGTGCCAACTGCAGCACGCTA 1506 GGGGTGGTCACCACGCGTGGCGGATGT RET NM_020630 7 10 + 105 − 0 37 1 0 CGCGAATGCCTTCACAAACAGGATCCCCGAGGT 1507 GTCCTCGGCTGAACGCGTGGCGGATGT RET NM_020630 7 10 + 140 + 0 37 1 0 CGCGAATGCCTGACACCAAGGCCCTGCGGCGGC 1508 CCAAGTGTGCCGACGCGTGGCGGATGT RET NM_020630 7 10 + 175 − 0 37 1 0 CGCGAATGCCCTGCTGGTCGGTGGCCACCACCA 1509 TGTAGTGAAGTTACGCGTGGCGGATGT RET NM_020630 7 10 + 210 + 0 37 1 0 CGCGAATGCCACCTCTAGGCAGGCCCAGGCCCA 1510 GCTGCTTGTAACACGCGTGGCGGATGT RET NM_020630 7 10 + 245 − 0 37 1 0 CGCGAATGCCCTCCCTGGAGCAGGCACTCACAT 1511 GACCCCTCCACTACGCGTGGCGGATGT EPHA7 NM_004440 3 6 − 0 + 0 37 1 0 CGCGAATGCCGTTATAAAAGCAATAGAAGAAG 1512 GTTATCGTTTACCACGCGTGGCGGATGT EPHA7 NM_004440 3 6 − 35 − 0 37 1 0 CGCGAATGCCGCTGGTGAAGGCCAGCTGGGCAG 1513 TCCATGGGTGCTACGCGTGGCGGATGT EPHA7 NM_004440 3 6 − 70 + 0 37 1 0 CGCGAATGCCTAATGTTGGATTGTTGGCAAAAG 1514 GAGCGTGCTGAAACGCGTGGCGGATGT EPHA7 NM_004440 3 6 − 105 − 0 37 1 0 CGCGAATGCCTCTAGAATTCCAACTATCTGTTCA 1515 AATTTTGGCCTACGCGTGGCGGATGT EPHA7 NM_004440 3 6 − 140 + 0 37 1 0 CGCGAATGCCCAAAATGATTCGAAACCCAAATA 1516 GTCTGAAAACTCACGCGTGGCGGATGT EPHA7 NM_004440 3 6 − 175 − 0 37 1 0 CGCGAATGCCCTTAGGCATTTCTTACCTACTACA 1517 AGTTCCCAGGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 0 + 0 37 1 0 CGCGAATGCCATGGCGAGTTGCTCCTTCACTCG 1518 CGACCAAGCGACACGCGTGGCGGATGT IRS4 NM_003604 1 X − 35 − 0 37 1 0 CGCGAATGCCCCGCTGCCGCCGCTGCTGCACCT 1519 CTTAGTCTTCTTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 70 + 0 37 1 0 CGCGAATGCCCAGCTCTAGCAGCAGTGGTGACC 1520 ACCCCGCTTCTTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 105 − 0 37 1 0 CGCGAATGCCCCGGTCCCAATGAGTGCGGTCGG 1521 GGTTCCCGAGGAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 140 + 0 37 1 0 CGCGAATGCCGTCGTCTTGTCCGGGAGCCATGT 1522 GGCTCTCCACGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 175 − 0 37 1 0 CGCGAATGCCCTCTTCGGACTCGGAGTCTGACC 1523 GGGAGCCAGTGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 210 + 0 37 1 0 CGCGAATGCCGAGGACCTGCCCGTCGGGGAGGA 1524 AGTCTGCAAACGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 245 − 0 37 1 0 CGCGAATGCCGCCTGTGCCCATGCTTCTGTTTCC 1525 GCAGGTAGCCGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 280 + 0 37 1 0 CGCGAATGCCGCTACTTCGTGCTCAAACTCGAG 1526 ACTGCTGACGCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 315 − 0 37 1 0 CGCGAATGCCTTCCTGGCATTTTCGTAGTATTCC 1527 AGCCGAGCTGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 350 + 0 37 1 0 CGCGAATGCCGTTCCGGCACAGTGTCCGCGCCG 1528 CGGCGGCTGCAGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 385 − 0 37 1 0 CGCGAATGCCCGGGGGGATCGCGGCGCCAGAG 1529 GCGGCCGCCGCTGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 420 + 0 37 1 0 CGCGAATGCCCTCATTCCACCGCGGCGCGTGAT 1530 CACCCTATACCAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 455 − 0 37 1 0 CGCGAATGCCACCTTGCATCTGCTCGCTGGCTCA 1531 CGGAAAAGCACACGCGTGGCGGATGT IRS4 NM_003604 1 X − 490 + 0 37 1 0 CGCGAATGCCACCGACACCTCATTGCTCTTTTCA 1532 CCCAAGACGAAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 525 − 0 37 1 0 CGCGAATGCCTGCTCCGACTCGTTCTCGGCCACC 1533 ATCGCGAAGTAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 560 + 0 37 1 0 CGCGAATGCCGGAAAGCTGGTACTTGCTGCTCA 1534 GCCGCCTCATCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 595 − 0 37 1 0 CGCGAATGCCGCCGAGCGTGCCGCAGCGGCGGC 1535 GCTTGCTCTCGAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 630 + 0 37 1 0 CGCGAATGCCGCGCAGCCGGACGGAGAGCCGG 1536 CCGCGCTGGCGGCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 665 − 0 37 1 0 CGCGAATGCCCATCTTTATAGAAGGGTGGCTCC 1537 GCCGCCGCTGCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 700 + 0 37 1 0 CGCGAATGCCTGTGGCAGGTAATAGTCAAACCC 1538 AGGGGGCTGGGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 735 − 0 37 1 0 CGCGAATGCCCACAGCCGGAACACGCCGCTCAG 1539 CTCTTTTCTGTGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 770 + 0 37 1 0 CGCGAATGCCTCTAACCGACGAGGAGGTCGTGT 1540 TTGTGAGGCTGAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 805 + 0 37 1 0 CGCGAATGCCCAGGAGCTGGACGACCACGCTGG 1541 CCACTTCGGTGTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 840 + 0 37 1 0 CGCGAATGCCAGCATCCGTCGCTGTGGACACTC 1542 GGAGCAGTATTTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 875 − 0 37 1 0 CGCGAATGCCGACCGATGACAGTGGACCTGCCT 1543 ACTTCCAAGAAGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 910 + 0 37 1 0 CGCGAATGCCCGGGAGAGCTCTGGATGCAGGTC 1544 GATGACTGTGTGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 945 − 0 37 1 0 CGCGAATGCCTTCTCCAAAAACAGCTCATGCAT 1545 GTTTTGGGCAACACGCGTGGCGGATGT IRS4 NM_003604 1 X − 980 + 0 37 1 0 CGCGAATGCCGATGAGAGCCTTGTGTGCAGACG 1546 AATACAGAGCCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1015 − 0 37 1 0 CGCGAATGCCGTGGGCGCCGATGCTGATGCTGT 1547 AGCTGCGGCAGCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1050 + 0 37 1 0 CGCGAATGCCCTGTTAACCCTGCTGTCCGCTAG 1548 GAGGCACCTGGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1085 − 0 37 1 0 CGCGAATGCCTTCTGAGCCAGCCTCCCGGCTCG 1549 AGCGGCACCAAGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1120 + 0 37 1 0 CGCGAATGCCGGTCCCGCTTTGAGCAGTTTTGCC 1550 ACCTCAGGGCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1155 − 0 37 1 0 CGCGAATGCCCTGGTGAAAAGCATCTCGTCTTC 1551 CCCGTCGCCGATACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1190 + 0 37 1 0 CGCGAATGCCGCGCTTCGTAACACCCAGCGAGC 1552 CTGTGGCCCACTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1225 − 0 37 1 0 CGCGAATGCCGCGCCCTCTGGGCAGGTGCAGTC 1553 TTCCTCGCCTGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1260 + 0 37 1 0 CGCGAATGCCAGGTCAAGGAGAGCGGTTTCAGT 1554 GCCGGCCAGCTTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1295 − 0 37 1 0 CGCGAATGCCGGGGACGTGCTGGGCTGGGTGCT 1555 AAGCGGCGAAAAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1330 + 0 37 1 0 CGCGAATGCCGGCACCCTGCAGAAGCCCCGAAC 1556 AATGGAGCTCGCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1365 − 0 37 1 0 CGCGAATGCCTTGCCAGAGCCAGAACCAGACAC 1557 TTCAGAAGACAGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1400 + 0 37 1 0 CGCGAATGCCCTTTGGGGAGGAAGGCAATCCCC 1558 AGGGCAAAGAAGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1435 − 0 37 1 0 CGCGAATGCCCATAGGCATGTAGTCACCTCCGC 1559 TTCCTTCCTGATACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1470 + 0 37 1 0 CGCGAATGCCAACAATTGGGGCTCAGGAAATGG 1560 CCGGGGCTCAGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1505 − 0 37 1 0 CGCGAATGCCTACTGGAGCCTTGGCCATTTGAG 1561 CCCTGGCCACCTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1540 + 0 37 1 0 CGCGAATGCCGCCATAGCTCGGGAGGAAACCAG 1562 TGTTCAGGCGAGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1575 − 0 37 1 0 CGCGAATGCCCCATTTGAGCCCTGACCACCTCG 1563 GGATCCCTGTCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1610 + 0 37 1 0 CGCGAATGCCCCAGGGCTCAGGAGGAAACCAGT 1564 GCTCTAGAGATGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1645 − 0 37 1 0 CGCGAATGCCACCACCTGAACCGTGCCCACCTG 1565 CGGTGCCCTGGCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1680 + 0 37 1 0 CGCGAATGCCGGCCAGAGACCTGGAGGTGGGC 1566 ATGGCTCAGGTGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1715 − 0 37 1 0 CGCGAATGCCCACCTGAGCCATGGCCATCTCCA 1567 GGTCCCTGGCCAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1750 + 0 37 1 0 CGCGAATGCCGTGGCAAGAACTCTGGGGGGGGC 1568 AAAGGCTCAGGAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1785 − 0 37 1 0 CGCGAATGCCCCACGTTCACCATCACCATCGGA 1569 TCCTTTCCCACTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1820 + 0 37 1 0 CGCGAATGCCAAAATCTCTGAAGAAAAGATCCT 1570 ATTTTGGCAAATACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1855 − 0 37 1 0 CGCGAATGCCAGGTGGTGGCATTTGCTGTTGCTT 1571 GCTTTGAGTTAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1890 + 0 37 1 0 CGCGAATGCCCCACCACCTCCTCCTCCACCCCCA 1572 CCAGCTGGAGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1925 − 0 37 1 0 CGCGAATGCCTGAATCTTCCCCCAGACTTCCCTT 1573 TTCCACCAGTTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1960 + 0 37 1 0 CGCGAATGCCGACTTTATTTTTGTGTTGACAGAG 1574 GAGCCACGAAAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 1995 − 0 37 1 0 CGCGAATGCCTCTGCATCTTTCACTTCTTTGGCT 1575 TCTTTGCATTCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2030 + 0 37 1 0 CGCGAATGCCGATCCCAGAAGGTGCAGCTCGAG 1576 GTCCCCACAGAGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2065 − 0 37 1 0 CGCGAATGCCGTATGGGTCATCCTCATCTTCATC 1577 AAAAGCTCTGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2100 + 0 37 1 0 CGCGAATGCCGTGCCAATGAGGCCAGGGGTGGC 1578 CACCCCTCTTGTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2135 − 0 37 1 0 CGCGAATGCCTTTGAGGAGCCATTGGCATATAA 1579 TCACTGGAGCTTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2170 + 0 37 1 0 CGCGAATGCCATGTCTCTGCTTCAAAAAAGCGC 1580 CACTCTCGATCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2205 − 0 37 1 0 CGCGAATGCCAACATCATCATGTACCCTCTTGA 1581 ATCTTCAAAAGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2240 + 0 37 1 0 CGCGAATGCCTCCCAGAGTGAGCCCACCACCTG 1582 CTCCGAGTCCTCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2275 − 0 37 1 0 CGCGAATGCCTGAGTCATCCTCTTTATTAGTATC 1583 AGGTGCTTTTGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2310 + 0 37 1 0 CGCGAATGCCAAGGACAATGACAGTGAGAGTG 1584 ACTACATGTTTATACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2345 − 0 37 1 0 CGCGAATGCCTGGGGTTTTTTGGAATTGCACCG 1585 GCTCCAGGAGCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2380 + 0 37 1 0 CGCGAATGCCGAAATCCTCAGGGTGGCTCTTCC 1586 TCCAAAAGTTGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2415 − 0 37 1 0 CGCGAATGCCCTCCGAAAAGGGTTTGGTAGAGA 1587 GAAGTAGGAGCTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2450 + 0 37 1 0 CGCGAATGCCCTCACCTTTGGGACAGAATGACA 1588 ACAGTGAGTATGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2485 − 0 37 1 0 CGCGAATGCCGCCCCTCCCCAGGAACTTTCCAG 1589 GTAACATTGGCAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2520 + 0 37 1 0 CGCGAATGCCCTAGACAAAGAAGTCTCCTATAA 1590 CTGGGACCCCAAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2555 − 0 37 1 0 CGCGAATGCCATGATCCCTCACCTGAAGGCTTT 1591 GAAGCTGCATCTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2590 + 0 37 1 0 CGCGAATGCCTCTCAAAGCCTGGAGATGGGGGA 1592 TCACCTTCAAAGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2625 − 0 37 1 0 CGCGAATGCCTTAGCTTTATTCTTTGGGGGCTCA 1593 TGATCTGAAGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2660 + 0 37 1 0 CGCGAATGCCGAGACCTAACCGACTTTCTTTTAT 1594 TACAAAAGGATACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2695 − 0 37 1 0 CGCGAATGCCATGTGTGGGCTTTTGTGGTTTTGG 1595 CTTGATTTTATACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2730 + 0 37 1 0 CGCGAATGCCGAGCAGAGAGAAGCTGACAGCT 1596 CTAGTGACTACGTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2765 − 0 37 1 0 CGCGAATGCCGTGTATTGCTCTCTCTTTTAGTGA 1597 AGTCCATGTTGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2800 + 0 37 1 0 CGCGAATGCCCAGCTCCCTCTACTCAAGGACTA 1598 CCAGATTCGTGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2835 − 0 37 1 0 CGCGAATGCCGAAAAGGCTGACTGTCTGGGTTC 1599 AGCAATTATGCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2870 + 0 37 1 0 CGCGAATGCCTAATTATGTGAATGTTGAGTTTG 1600 GAGTGCCATTTCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2905 − 0 37 1 0 CGCGAATGCCTCTTAAAAGATCTGAGAGGTCGT 1601 TTGCTGGATTTGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2940 + 0 37 1 0 CGCGAATGCCGCTATACCACGTGCCAACCCCTT 1602 ATCTCTGGACAGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 2975 − 0 37 1 0 CGCGAATGCCCACTGAGGGGAAGGGGAGGAAG 1603 TGGCCACCTAGCAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3010 + 0 37 1 0 CGCGAATGCCCTACAGGTAGCAATGCTATTGAG 1604 GAAGAGGGTGACACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3045 − 0 37 1 0 CGCGAATGCCGGTGTCATTGCTGAGTTGAAAAT 1605 TACTTCAATGTAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3080 + 0 37 1 0 CGCGAATGCCAGCCATGGCTCTTGCTGACAGTG 1606 CCATTCGCTATGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3115 − 0 37 1 0 CGCGAATGCCTGGGTCGACCACATAGATTCGAC 1607 CTGTTTCAGCATACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3150 + 0 37 1 0 CGCGAATGCCTTTTCTGAGTGCTGTATGGATATT 1608 TCTCTCTCCCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3185 − 0 37 1 0 CGCGAATGCCGCCTAGCTACAGGTGGTGGTTCA 1609 GAACATCGGCTGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3220 + 0 37 1 0 CGCGAATGCCTGCTGCAGGAAGAAGAGCAGGA 1610 GAGAAGACGCCCAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3255 − 0 37 1 0 CGCGAATGCCCTGGCTGCTGCAAAGAAACTTTG 1611 AGAACGGCTTTGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3290 + 0 37 1 0 CGCGAATGCCAGCCGCTGTCTCTGCTTTTCCAAC 1612 AGACAGCCTCGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3325 − 0 37 1 0 CGCGAATGCCGACAGCCGGGGCTGAGGATGGG 1613 GAAAGGTCTCTCTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3360 + 0 37 1 0 CGCGAATGCCGCTTCGGCTGCAGAGCCGACTTT 1614 AGCCCTCAGCCAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3395 − 0 37 1 0 CGCGAATGCCGGGCTGCGGCGAGCGCGGAGGC 1615 CGCAGCTACAACTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3430 + 0 37 1 0 CGCGAATGCCCGGGCATCGGCGCAGCAGCCGCA 1616 GCTGCTGGATTTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3465 − 0 37 1 0 CGCGAATGCCGCAACAGGTTGAAACCAGCGGGC 1617 AGAGGCGGAGTCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3500 + 0 37 1 0 CGCGAATGCCTAATGCTGCTGATGCCGAAGCAG 1618 TAAGGGGAGCCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3535 − 0 37 1 0 CGCGAATGCCGTGGGCTCCAGGGTTCGAGCCAC 1619 CGGCAACGTCTTACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3570 + 0 37 1 0 CGCGAATGCCAACCCATCTGCAAACCTTGCCAG 1620 AGGTGATAACCAACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3605 − 0 37 1 0 CGCGAATGCCCCGGAGCGGCAGCTGCAGCGGCA 1621 GCCCCGCCAGCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3640 + 0 37 1 0 CGCGAATGCCAACCACCACCTCGCAGTCGCCGG 1622 GTGCCAAGACCCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3675 − 0 37 1 0 CGCGAATGCCGTGTCGTCGTCGTTGTCAGAATCT 1623 TCTCTCTCCGGACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3710 + 0 37 1 0 CGCGAATGCCTCACGTGAGAATGGATTTTGCCA 1624 GACGTGATAATCACGCGTGGCGGATGT IRS4 NM_003604 1 X − 3745 − 0 37 1 0 CGCGAATGCCCTAAAATTACCGACCTCTTTTGG 1625 GAGAGTCGAACTACGCGTGGCGGATGT RET NM_020630 1 10 + −34 + 0 37 1 0 CGCGAATGCCGTCCCTCCAGCCGTGGCCCCAGC 1626 GCGCACGGGCGAACGCGTGGCGGATGT RET NM_020630 1 10 + 1 − 0 37 1 0 CGCGAATGCCACGCAGCCCCGCGGCACCGGACG 1627 TCGCCTTCGCCAACGCGTGGCGGATGT RET NM_020630 1 10 + 40 + 25 37 1 0 CGCGAATGCCTGTTGCTGCTGCTGCTGCCGCTGC 1628 TAGGCAAAGGTACGCGTGGCGGATGT RET NM_020630 1 10 + 71 − 0 37 1 0 CGCGAATGCCCCCCTGCGGGAGCCGGCGGCCGG 1629 CAGAACTCACCTACGCGTGGCGGATGT EPHA3 NM_005233 14 3 + 0 + 0 37 1 0 CGCGAATGCCGGAGGGAAGATCCCAATCAGGTG 1630 GACATCACCAGAACGCGTGGCGGATGT EPHA3 NM_005233 14 3 + 35 − 0 37 1 0 CGCGAATGCCCGCTGGCTGACGTGAACTTGCGG 1631 TAGGCTATAGCTACGCGTGGCGGATGT EPHA3 NM_005233 14 3 + 70 + 0 37 1 0 CGCGAATGCCATGTATGGAGTTATGGGATTGTT 1632 CTCTGGGAGGTGACGCGTGGCGGATGT EPHA3 NM_005233 14 3 + 105 − 0 37 1 0 CGCGAATGCCGACATCTCCCAGTATGGTCTCTCT 1633 CCATAAGACATACGCGTGGCGGATGT EPHA3 NM_005233 14 3 + 140 + 0 37 1 0 CGCGAATGCCCAATCAGGATGTAAGTATTTGTG 1634 GTCTATGAGTTAACGCGTGGCGGATGT RB1 NM_000321 2 13 + −6 + 0 37 1 0 CGCGAATGCCTGGTAGGCTTGAGTTTGAAGAAA 1635 CAGAAGAACCTGACGCGTGGCGGATGT RB1 NM_000321 2 13 + 29 − 0 37 1 0 CGCGAATGCCTGGTATCTTTAATTTCTGACATAA 1636 TGCAGTAAAATACGCGTGGCGGATGT RB1 NM_000321 2 13 + 64 + 0 37 1 0 CGCGAATGCCGATCATGTCAGAGAGAGAGCTTG 1637 GTTAACTTGGGAACGCGTGGCGGATGT RB1 NM_000321 2 13 + 99 − 0 37 1 0 CGCGAATGCCTCCTTACCAATACTCCATCCACA 1638 GATGAAACTTTCACGCGTGGCGGATGT RB1 NM_000321 7 13 + −14 + 0 37 1 0 CGCGAATGCCCATTTTTTTTTCAGGGGAAGTATT 1639 ACAAATGGAAGACGCGTGGCGGATGT RB1 NM_000321 7 13 + 21 − 0 37 1 0 CGCGAATGCCACATAGCATTAACTGAAATGAAA 1640 TCACCAGATCATACGCGTGGCGGATGT RB1 NM_000321 7 13 + 56 + 0 37 1 0 CGCGAATGCCGTCCTTGACTATTTTATTAAACTC 1641 TCACCTCCCATACGCGTGGCGGATGT RB1 NM_000321 7 13 + 91 − 0 37 1 0 CGCGAATGCCAAATTAAATACTTACTATATGGT 1642 TCTTTGAGCAACACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 0 + 0 37 1 0 CGCGAATGCCGTTTCACCGTTGTTTGGAACCATC 1643 TACAATTCAATACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 35 − 0 37 1 0 CGCGAATGCCTCATAGCATTATTCATGGCTTGTG 1644 CGATAAAGTAAACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 70 + 0 37 1 0 CGCGAATGCCAAGAAAATGGACAGGCTGGTGCT 1645 GCCAGCCTGGTTACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 105 − 0 37 1 0 CGCGAATGCCTTGAATCCATGGAACTGCATGTT 1646 TCTGGAATGCTGACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 140 + 0 37 1 0 CGCGAATGCCCCAGTTGATGAGGACAGATTCAA 1647 ATGGAAATGGAAACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 175 − 0 37 1 0 CGCGAATGCCTTTCAAGTTGGTGTCCAGGATTA 1648 CATATTCTGAAAACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 210 + 0 37 1 0 CGCGAATGCCGAATGGGAACTCCATAGCACCTA 1649 CACTGTGGACATACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 245 − 0 37 1 0 CGCGAATGCCTAGGGGTCCCTCCGAAACGTAGC 1650 AGCTCCATTTCCACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 280 + 0 37 1 0 CGCGAATGCCTTCACTTCCCTGGTGGCAGGCCC 1651 CCTAGAGCAGATACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 315 − 0 37 1 0 CGCGAATGCCTGGCAGATCTTCCCTTCTGCAAA 1652 CCAGCATTTTGCACGCGTGGCGGATGT GUCY2F NM_001522 17 X − 350 + 0 37 1 0 CGCGAATGCCTGGAGGTAAGGAATGCAAAATCA 1653 CTAGTAGTCAATACGCGTGGCGGATGT EPHA4 NM_004438 13 2 − −8 + 0 37 1 0 CGCGAATGCCTCTAACAGCACCATCATCCATTG 1654 CTTTGGTCCAGGACGCGTGGCGGATGT EPHA4 NM_004438 13 2 − 27 − 0 37 1 0 CGCGAATGCCAGCCAGTGCCACACTGTATCTTG 1655 TGACTTCTTTAGACGCGTGGCGGATGT EPHA4 NM_004438 13 2 − 62 + 0 37 1 0 CGCGAATGCCTGGCTGGAACCAGATCGGCCCAA 1656 TGGGGTAATCCTACGCGTGGCGGATGT EPHA4 NM_004438 13 2 − 97 − 0 37 1 0 CGCGAATGCCGTAATACCTTCTCATAATACTTGA 1657 CTTCATATTCCACGCGTGGCGGATGT GUCY2F NM_001522 15 X − −22 + 0 37 1 0 CGCGAATGCCTCTGATTTCATGTTTGTTTTAGGC 1658 GTCGTATAAATACGCGTGGCGGATGT GUCY2F NM_001522 15 X − 13 − 0 37 1 0 CGCGAATGCCAGAATTCTATTGGGTCCTTTGATC 1659 AACTGGATTTTACGCGTGGCGGATGT GUCY2F NM_001522 15 X − 48 + 0 37 1 0 CGCGAATGCCACTGACTTTGGAGGATGTAACGT 1660 TTATCAATCCCCACGCGTGGCGGATGT GUCY2F NM_001522 15 X − 83 − 0 37 1 0 CGCGAATGCCAAAGAAGAGGATACTCCTTACCT 1661 TACTGCCAAAGTACGCGTGGCGGATGT GUCY2F NM_001522 12 X − 0 + 0 37 1 0 CGCGAATGCCATGAAGGACTTGCGTCATGAGAA 1662 TATTAACCCTTTACGCGTGGCGGATGT GUCY2F NM_001522 12 X − 35 − 0 37 1 0 CGCGAATGCCTGGCAAACATCCCCGAATCATAG 1663 AAGAAACCCAATACGCGTGGCGGATGT GUCY2F NM_001522 12 X − 70 + 0 37 1 0 CGCGAATGCCTTGTGACAGAATTCTGTTCCCGA 1664 GGGAGCCTAGAAACGCGTGGCGGATGT GUCY2F NM_001522 12 X − 105 − 0 37 1 0 CGCGAATGCCCAGTCAAGTTTCACATCTTGATTT 1665 GTCAGTATGTCACGCGTGGCGGATGT GUCY2F NM_001522 12 X − 140 + 0 37 1 0 CGCGAATGCCGATGTTTAAATCATCACTCTTGCT 1666 GGATCTCATAAACGCGTGGCGGATGT GUCY2F NM_001522 12 X − 175 − 0 37 1 0 CGCGAATGCCAAAATCCAAGATTTCCTCAGTCT 1667 TCCCATTAACCTACGCGTGGCGGATGT KSR2 NM_173598 9 12 − −34 + 0 37 1 0 CGCGAATGCCTTTTTTTTCTGAGTGTTCAATCTC 1668 TGGTTTTCAGAACGCGTGGCGGATGT KSR2 NM_173598 9 12 − 1 − 0 37 1 0 CGCGAATGCCGCGCCCGGGTCGGCGTCTCCGGC 1669 ACCGGCACCACAACGCGTGGCGGATGT KSR2 NM_173598 9 12 − 36 + 0 37 1 0 CGCGAATGCCCCCAGGTCATCCTGCATCCGGTG 1670 ACCTCGAATCCAACGCGTGGCGGATGT KSR2 NM_173598 9 12 − 71 − 0 37 1 0 CGCGAATGCCGGGAAAGGGGGAAGATGTCTCTG 1671 TCTCACTTACATACGCGTGGCGGATGT RBBP8 NM_002894 16 18 + −35 + 0 37 1 0 CGCGAATGCCACATTTAGTTTGTAATGTGCATGT 1672 TTTATTTATAGACGCGTGGCGGATGT RBBP8 NM_002894 16 18 + 0 − 0 37 1 0 CGCGAATGCCTTTCTGCTTGACTTTGTCTTGTTT 1673 ATCACCATGAGACGCGTGGCGGATGT RBBP8 NM_002894 16 18 + 35 + 0 37 1 0 CGCGAATGCCGCGTTTGTGGAGCCGTATTTTAA 1674 AGGTGATGAAAGACGCGTGGCGGATGT RBBP8 NM_002894 16 18 + 70 − 0 37 1 0 CGCGAATGCCAGTCACATTGCTGGTATAAATAA 1675 AAACCAACTTACACGCGTGGCGGATGT PDGFRA NM_006206 18 4 + −8 + 0 37 1 0 CGCGAATGCCCCATGCAGTGTGTCCACCGTGAT 1676 CTGGCTGCTCGCACGCGTGGCGGATGT PDGFRA NM_006206 18 4 + 27 − 0 37 1 0 CGCGAATGCCATCTTCACAATTTTTCCTTGTGCC 1677 AGGAGGACGTTACGCGTGGCGGATGT PDGFRA NM_006206 18 4 + 62 + 0 37 1 0 CGCGAATGCCCTGTGACTTTGGCCTGGCCAGAG 1678 ACATCATGCATGACGCGTGGCGGATGT PDGFRA NM_006206 18 4 + 97 − 0 37 1 0 CGCGAATGCCAGGACGTACACTGCCTTTCGACA 1679 CATAGTTCGAATACGCGTGGCGGATGT RPS6KA1 NM_002953 22 1 + −8 + 0 37 1 0 CGCGAATGCCTCTTTCAGGGAGCCATGGCTGCC 1680 ACGTACTCCGCAACGCGTGGCGGATGT RPS6KA1 NM_002953 22 1 + 27 − 0 37 1 0 CGCGAATGCCGGCTTCAGCTGGGGGGTGGGCTT 1681 GGAGCTGTTGAGACGCGTGGCGGATGT RPS6KA1 NM_002953 22 1 + 62 + 0 37 1 0 CGCGAATGCCCATCGAGTCATCCATCCTGGCCC 1682 AGCGGCGAGTGAACGCGTGGCGGATGT RPS6KA1 NM_002953 22 1 + 97 − 0 37 1 0 CGCGAATGCCCCTGGTGCCTCACAGGGTGGTGG 1683 ATGGCAACTTCCACGCGTGGCGGATGT EPHA3 NM_005233 1 3 + −26 + 0 37 1 0 CGCGAATGCCCTCTCACTGCCCTCTGCACCAGC 1684 AACATGGATTGTACGCGTGGCGGATGT EPHA3 NM_005233 1 3 + 9 − 0 37 1 0 CGCGAATGCCACAGAGCAGCTGAGAAGGAGGA 1685 GGATGGAGAGCTGACGCGTGGCGGATGT EPHA3 NM_005233 1 3 + 44 + 0 37 1 0 CGCGAATGCCTCTCGACAGCTTCGGGGAACTGA 1686 TTCCGCAGCCTTACGCGTGGCGGATGT EPHA3 NM_005233 1 3 + 79 − 0 37 1 0 CGCGAATGCCTCCGTGCGTCGCGGTACCTGGCT 1687 TACCTTCATTGGACGCGTGGCGGATGT PIK3CA NM_006218 16 3 + −9 + 0 37 1 0 CGCGAATGCCATTTTAAAGGCTTGAAGAGTGTC 1688 GAATTATGTCCTACGCGTGGCGGATGT PIK3CA NM_006218 16 3 + 26 − 0 37 1 0 CGCGAATGCCGTTCTCCCAATTCAACCACAGTG 1689 GCCTTTTTGCAGACGCGTGGCGGATGT PIK3CA NM_006218 16 3 + 61 + 0 37 1 0 CGCGAATGCCCCAGACATCATGTCAGAGTTACT 1690 GTTTCAGAACAAACGCGTGGCGGATGT PIK3CA NM_006218 16 3 + 96 − 0 37 1 0 CGCGAATGCCCTTCCTTACCATCCCCATTTTTAA 1691 AGATGATCTCAACGCGTGGCGGATGT EPHB1 NM_004441 4 3 + 0 + 0 37 1 0 CGCGAATGCCCTTGCCCTGCAGGGACATTCAAG 1692 GCCAGCCAGGAAACGCGTGGCGGATGT EPHB1 NM_004441 4 3 + 35 − 0 37 1 0 CGCGAATGCCCGGCTGTTGGAGGGGCAGTGGGA 1693 GCAGCCTTCAGCACGCGTGGCGGATGT EPHB1 NM_004441 4 3 + 70 + 0 37 1 0 CGCGAATGCCCTCCCCTGCAGAGGCGTCTCCCA 1694 TCTGCACCTGTCACGCGTGGCGGATGT EPHB1 NM_004441 4 3 + 105 − 0 37 1 0 CGCGAATGCCTGGAGGGTCAAAGTCCGCTCGGT 1695 AATAACCGGTCCACGCGTGGCGGATGT EPHB1 NM_004441 4 3 + 140 + 0 37 1 0 CGCGAATGCCGAAGTGGCATGCACTAGTAAGTG 1696 TCTAGTAATGGCACGCGTGGCGGATGT KSR2 NM_173598 2 12 − −3 + 0 37 1 0 CGCGAATGCCTAGGACATTCTTCTCTTCTGCTGG 1697 GCCTTTGAACAACGCGTGGCGGATGT KSR2 NM_173598 2 12 − 32 − 0 37 1 0 CGCGAATGCCTGTCCATGAGCTTGGTGAAGGTA 1698 GGTCTCTCTTCTACGCGTGGCGGATGT KSR2 NM_173598 2 12 − 67 + 0 37 1 0 CGCGAATGCCTGCTGGAGAAACTGCCAAAGCGA 1699 AACCGTCGCCTGACGCGTGGCGGATGT KSR2 NM_173598 2 12 − 102 − 0 37 1 0 CGCGAATGCCTACTCTGCAGACTTCCAGAAATG 1700 TCCAGGGTGAGAACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 0 + 0 37 1 0 CGCGAATGCCGAGGCAACCTTTCCAAACAAGAC 1701 TGGACCATCCAGACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 35 − 0 37 1 0 CGCGAATGCCGGATTGTTCTCCTTCCCCGTCTCT 1702 GTCGTGGGCCAACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 70 + 0 37 1 0 CGCGAATGCCCGTGTGCCCCCCGGAGCCCACCC 1703 CGTGGATCCGCAACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 105 − 0 37 1 0 CGCGAATGCCCTTGGACGGGACCCTGGGGCTCT 1704 GGGAGAGATGGGACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 140 + 0 37 1 0 CGCGAATGCCTGCGTCCAGCACTATTGTCACAC 1705 CAGCCCCACTCCACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 175 − 0 37 1 0 CGCGAATGCCTAAGCCTGTCCACGTGGGTGTAC 1706 ACAGGGGCCCCGACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 210 + 0 37 1 0 CGCGAATGCCCCGTGGACGCCTACCCGGGCTTG 1707 TGCCCGCCCCCGACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 245 − 0 37 1 0 CGCGAATGCCGATGGGGGCAGGGAACGGTGGC 1708 CCGACTCCAGTGGACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 280 + 0 37 1 0 CGCGAATGCCGCCCCGGCAGCGGCACGCGGTCC 1709 GCACCCCGCCGCACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 315 − 0 37 1 0 CGCGAATGCCCGGCGGGGTCACGGTGGTGACGA 1710 TGTTGGGGGTGCACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 350 + 0 37 1 0 CGCGAATGCCGGCACGCCGCCCATGAGGAAGA 1711 AGAACAAGCTGAAACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 385 − 0 37 1 0 CGCGAATGCCGTTTTCGGGAGGAGGGCGGTGGG 1712 GTCCCCGGGGGCACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 420 + 0 37 1 0 CGCGAATGCCTGATACACTTGATCCCGGGATTC 1713 ACCGCGCTGCATACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 455 − 0 37 1 0 CGCGAATGCCCGGTGCCCCAGCTGGAACTCGTG 1714 GGATTTGCTCCGACGCGTGGCGGATGT KSR2 NM_173598 17 12 − 490 + 0 37 1 0 CGCGAATGCCCGTGGACGAGGCCCACACGCCCA 1715 AGTGAGTGCGATACGCGTGGCGGATGT KSR2 NM_173598 10 12 − −49 + 0 37 1 0 CGCGAATGCCCGATGTCTCCCTGGGGACACTCA 1716 CTTTGATGTTTTACGCGTGGCGGATGT KSR2 NM_173598 10 12 − −14 − 0 37 1 0 CGCGAATGCCTGTATTTGTAGTAGTGGGATGCTT 1717 GCAAAAGAAAGACGCGTGGCGGATGT KSR2 NM_173598 10 12 − 21 + 0 37 1 0 CGCGAATGCCAGCAGCAGTTCATCTTCCCAGGT 1718 GAGTTTCATGTGACGCGTGGCGGATGT KSR2 NM_173598 10 12 − 56 − 0 37 1 0 CGCGAATGCCCAGCGGGGCTTGTTCAGAAGGGG 1719 CTGCTTCCAGCTACGCGTGGCGGATGT EPHA3 NM_005233 8 3 + −18 + 0 37 1 0 CGCGAATGCCTTCTCTTCAACCTCACAGCTTTCT 1720 CCATCTCTGGTACGCGTGGCGGATGT EPHA3 NM_005233 8 3 + 17 − 0 37 1 0 CGCGAATGCCGCTGAAATGGCGATCATGACCAC 1721 TTGGCTACTTTCACGCGTGGCGGATGT EPHA3 NM_005233 8 3 + 52 + 0 37 1 0 CGCGAATGCCGGCAGTAGCAATTATTCTCCTCA 1722 CTGTTGTCATCTACGCGTGGCGGATGT EPHA3 NM_005233 8 3 + 87 − 0 37 1 0 CGCGAATGCCAAACAGACTGTGAACTCACCTCC 1723 CAATCAAAACATACGCGTGGCGGATGT KSR2 NM_173598 19 12 − 0 + 0 37 1 0 CGCGAATGCCAGCAAGCTGGTGAAGTACTTCAG 1724 CCGGCAGCTGTCACGCGTGGCGGATGT KSR2 NM_173598 19 12 − 35 − 0 37 1 0 CGCGAATGCCCGTTGCGCTCCTGCAAGGCTACC 1725 TTCTTTTTGCAGACGCGTGGCGGATGT KSR2 NM_173598 19 12 − 70 + 0 37 1 0 CGCGAATGCCCGGAGCTGGACGGCTTCCCCCAG 1726 CTACGGCACTGGACGCGTGGCGGATGT KSR2 NM_173598 19 12 − 105 − 0 37 1 0 CGCGAATGCCTCCAGGACCTCCTTGCGCACATC 1727 GACGATTCGGAAACGCGTGGCGGATGT KSR2 NM_173598 19 12 − 140 + 0 37 1 0 CGCGAATGCCGGTGACCTGGGGGGCCCCTGTGT 1728 CCCCTGCCCCTTACGCGTGGCGGATGT NTRK3 NM_001012338 1 15 − 0 + 0 37 1 0 CGCGAATGCCGTCATTGAGTGCATTACCCAAGG 1729 TCGTGTTTTGGAACGCGTGGCGGATGT NTRK3 NM_001012338 1 15 − 35 − 0 37 1 0 CGCGAATGCCCATCGTACACCTCTTTGGGGCAG 1730 ACTCGGGGCCGCACGCGTGGCGGATGT NTRK3 NM_001012338 1 15 − 70 + 0 37 1 0 CGCGAATGCCTCATGCTGGGGTGCTGGCAGAGG 1731 GAACCACAGCAGACGCGTGGCGGATGT NTRK3 NM_001012338 1 15 − 105 − 0 37 1 0 CGCGAATGCCTGGAGGATTTTGTAGATCTCCTTG 1732 ATGTTCAACCGACGCGTGGCGGATGT NTRK3 NM_001012338 1 15 − 140 + 0 37 1 0 CGCGAATGCCTGCTTTGGGGAAGGCCACCCCAA 1733 TCTACCTGGACAACGCGTGGCGGATGT NTRK3 NM_001012338 1 15 − 175 − 0 37 1 0 CGCGAATGCCTGAATTCATGACCACCAGCCACC 1734 ACTAGCCAAGAAACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 0 + 0 37 1 0 CGCGAATGCCGGTGTGCTGGGCGATGCCAGGAG 1735 TGGGAAGTCATCACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 35 − 0 37 1 0 CGCGAATGCCCCTGGTATGAGCCAGTCAGGAAT 1736 CGGTGGATGAGCACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 70 + 0 37 1 0 CGCGAATGCCTGCTGGAGAAGACAGAGAGTGA 1737 GTTCTGAAGAGCCACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 105 − 0 37 1 0 CGCGAATGCCTCCTGGATCCCTGACCCCAATGG 1738 TTAGCTTACTATACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 140 + 0 37 1 0 CGCGAATGCCGTGAGATCAATGATTAGGCAGGT 1739 GTGGAGACCAGGACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 175 − 0 37 1 0 CGCGAATGCCCCAGTGCTTTTGCTATTATGGCCT 1740 CATCCTTCCCCACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 210 + 0 37 1 0 CGCGAATGCCGAGCATGGGGAGGTTTGGTGACC 1741 TGTCACCTCTGAACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 245 − 0 37 1 0 CGCGAATGCCCTCACCTGTCCAGAAGAGTTGGA 1742 AGGGGTAACAGGACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 280 + 0 37 1 0 CGCGAATGCCCAGTACAAGAAAGAAATGTTGGT 1743 GGATGGACAGACACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 315 − 0 37 1 0 CGCGAATGCCGTGCCCCAGCTTCCTCTCGGATTA 1744 GCACCAGATGTACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 350 + 0 37 1 0 CGCGAATGCCCTGATGCCAAGGTGAGGGTGGAG 1745 GTGGTGGGGGACACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 385 − 0 37 1 0 CGCGAATGCCCCTCTCCTTTGGCCAGTGCTGACC 1746 CCAGCCAGCCTACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 420 + 0 37 1 0 CGCGAATGCCGCTCTGGTTTCCCAGGTCTTCATC 1747 TGGATTGGGTCACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 455 − 0 37 1 0 CGCGAATGCCGAGCAAATGGAAAGTCAGACAG 1748 GTCTACTCCTCTGACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 490 + 0 37 1 0 CGCGAATGCCCCTCACTCCCCGCAAGTTCTCAG 1749 GCTGGGCAGATGACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 525 − 0 37 1 0 CGCGAATGCCGTTCTCATCCTCCAGGCTGAAGA 1750 CGAAGATCACAGACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 560 + 0 37 1 0 CGCGAATGCCAGTTTCCAGGCTGTGAGCCGTCT 1751 CCATGGGCAGCTACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 595 − 0 37 1 0 CGCGAATGCCCCAGGCCTCCTCGTCCCTCCCCGC 1752 GAAGGGAACTCACGCGTGGCGGATGT CENTG1 NM_014770 16 12 − 630 + 0 37 1 0 CGCGAATGCCCCTTGGCACTGGTGGGGACACAA 1753 GGTAAGGAGGGGACGCGTGGCGGATGT RPS6KA1 NM_002953 3 1 + −12 + 0 37 1 0 CGCGAATGCCTCCCTCCATCAGGATGAGGGCGT 1754 CCTCAAGGAGATACGCGTGGCGGATGT RPS6KA1 NM_002953 3 1 + 23 − 0 37 1 0 CGCGAATGCCTCTCAGAGCCAGCCTTGACGTGG 1755 TGCGTGATGGAGACGCGTGGCGGATGT RPS6KA1 NM_002953 3 1 + 58 + 0 37 1 0 CGCGAATGCCAGGCTGATCCATCCCATTTCGAG 1756 CTCCTCAAGGTTACGCGTGGCGGATGT RPS6KA1 NM_002953 3 1 + 93 − 0 37 1 0 CGCGAATGCCTCATGACTCACTTTGCCAAAGGA 1757 TCCCTGGCCCAGACGCGTGGCGGATGT PIK3CA NM_006218 14 3 + 0 + 0 23 1 1 CGCGAATGCCATCTGAGATGCACAATAAAACAG 1758 TTAGCCAGAGGTACGCGTGGCGGATGT PIK3CA NM_006218 14 3 + 35 − 0 0 2 0 CGCGAATGCCACATGCACGACAATAGGACTCCA 1759 AAAGCAGGCCAAACGCGTGGCGGATGT PIK3CA NM_006218 14 3 + 70 + 0 0 2 0 CGCGAATGCCGGGATGTATTTGAAGCACCTGAA 1760 TAGGCAAGTCGAACGCGTGGCGGATGT PIK3CA NM_006218 14 3 + 105 − 0 23 1 1 CGCGAATGCCGAATGTCAGTTAAGTTAATGAGC 1761 TTTTCCATTGCCACGCGTGGCGGATGT PIK3CA NM_006218 14 3 + 139 + 0 37 1 0 CGCGAATGCCCTCAAACAGGAGAAGAAGGATG 1762 AAACACAAAAGGTACGCGTGGCGGATGT EPHA7 NM_004440 7 6 − 0 + 0 37 1 0 CGCGAATGCCGAGAATTCGGTGAAGTCTGCAGT 1763 GGCCGTTTGAAAACGCGTGGCGGATGT EPHA7 NM_004440 7 6 − 35 − 0 37 1 0 CGCGAATGCCTTTATGGCTACTGCAACATCTCTT 1764 TTCCCTGGAAGACGCGTGGCGGATGT EPHA7 NM_004440 7 6 − 70 + 0 37 1 0 CGCGAATGCCAACCCTGAAAGTTGGTTACACAG 1765 AAAAACAAAGGAACGCGTGGCGGATGT EPHA7 NM_004440 7 6 − 105 − 0 37 1 0 CGCGAATGCCCTGCCCCATGATGCTTGCTTCACA 1766 CAAAAAGTCTCACGCGTGGCGGATGT EPHA7 NM_004440 7 6 − 140 + 0 37 1 0 CGCGAATGCCTTTGACCACCCGAATGTTGTCCAT 1767 TTGGAAGGGGTACGCGTGGCGGATGT EPHA7 NM_004440 7 6 − 175 − 0 37 1 0 CGCGAATGCCAATAAAGATATAACCAATATCTA 1768 CCTCTTGTAACAACGCGTGGCGGATGT CENTG1 NM_014770 18 12 − 0 + 0 37 1 0 CGCGAATGCCATGCATGCCCAGAGGCAGTTCGT 1769 TGTAGCTGCAGTACGCGTGGCGGATGT CENTG1 NM_014770 18 12 − 35 − 0 37 1 0 CGCGAATGCCGCTTGGCCACCTCATGTCGTCTG 1770 ACTTCTGCTCTCACGCGTGGCGGATGT CENTG1 NM_014770 18 12 − 70 + 0 37 1 0 CGCGAATGCCAGGCTCTAAACCGCCTCAGGAAG 1771 CTGGCAGAGAGGACGCGTGGCGGATGT CENTG1 NM_014770 18 12 − 105 − 0 37 1 0 CGCGAATGCCGCCTGGATGCTGTCCTGGAGTTC 1772 GGGGTCGTCCACACGCGTGGCGGATGT CENTG1 NM_014770 18 12 − 140 + 0 37 1 0 CGCGAATGCCCTCATTGGACAGCATTCGAGGTA 1773 AGAGAAAGGTCAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 0 + 0 37 1 0 CGCGAATGCCATGGCGAGCCCTCCGGAGAGCGA 1774 TGGCTTCTCGGAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 35 − 0 37 1 0 CGCGAATGCCTCTTGGGTTTGCGCAGGTAGCCC 1775 ACCTTGCGCACGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 70 + 0 37 1 0 CGCGAATGCCGCATGCACAAACGCTTCTTCGTA 1776 CTGCGCGCGGCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 105 − 0 37 1 0 CGCGAATGCCTAGTACTCGAGGCGCGCCGGGCC 1777 CCCAGCCTCGCTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 140 + 0 37 1 0 CGCGAATGCCCGAGAACGAGAAGAAGTGGCGG 1778 CACAAGTCGAGCGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 175 − 0 37 1 0 CGCGAATGCCGAAGCAGCTCTCAAGGGGGATCG 1779 AGCGTTTGGGGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 210 + 0 37 1 0 CGCGAATGCCAACATCAACAAGCGGGCTGACTC 1780 CAAGAACAAGCAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 245 − 0 37 1 0 CGCGAATGCCCAAAGTGCTCGTCCCGGGTGTAG 1781 AGAGCCACCAGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 280 + 0 37 1 0 CGCGAATGCCCCATCGCGGCGGACAGCGAGGCC 1782 GAGCAAGACAGCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 315 − 0 37 1 0 CGCGAATGCCGCACGGTTGTGCAGCTGTAGGAG 1783 AGCCTGGTACCAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 350 + 0 37 1 0 CGCGAATGCCTAAGGGCCACCACGACGGAGCTG 1784 CGGCCCTCGGGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 385 − 0 37 1 0 CGCGAATGCCGGAGCTGCCGCTGCAGCTGCCCC 1785 CACCACCTCCCGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 420 + 0 37 1 0 CGCGAATGCCGGCCTTGGTGAGGCTGGGGAGGA 1786 CTTGAGCTACGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 455 − 0 37 1 0 CGCGAATGCCAGACCTCTTTGAATGCGGGTCCT 1787 GGGGGCACGTCAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 490 + 0 37 1 0 CGCGAATGCCGGCAAGTGATCCTGAAGCCCAAG 1788 GGCCTGGGTCAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 525 − 0 37 1 0 CGCGAATGCCAGGCAAAGGCGGTAGATACCAAT 1789 CAGGTTCTTTGTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 560 + 0 37 1 0 CGCGAATGCCGACCAGCAAGACCATCAGCTTCG 1790 TGAAGCTGAACTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 595 − 0 37 1 0 CGCGAATGCCGTTCATCAGCTGCAGCACCACGG 1791 CCGCTGCCTCCGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 630 + 0 37 1 0 CGCGAATGCCATCAGGCGCTGTGGCCACTCGGA 1792 AAACTTCTTCTTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 665 − 0 37 1 0 CGCGAATGCCCGGGCCCCGTCACGGCAGAACGG 1793 CCCACCTCGATGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 700 + 0 37 1 0 CGCGAATGCCGGGAGTTCTGGATGCAGGTGGAT 1794 GACTCTGTGGTGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 735 − 0 37 1 0 CGCGAATGCCATGGCCTCCAGGATGGTCTCGTG 1795 CATGTTCTGGGCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 770 + 0 37 1 0 CGCGAATGCCGCGGGCCATGAGTGATGAGTTCC 1796 GCCCTCGCAGCAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 805 − 0 37 1 0 CGCGAATGCCGATGGGGTTAGAGCAGTTGGACG 1797 AGGACTGGCTCTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 840 + 0 37 1 0 CGCGAATGCCAGCGTCCCCCTGCGCCGGCACCA 1798 TCTCAACAATCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 875 − 0 37 1 0 CGCGAATGCCGTGATCGGCGGGTCAGCCCCACC 1799 TGGCTGGGCGGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 910 + 0 37 1 0 CGCGAATGCCGCACTGAGAGCATCACCGCCACC 1800 TCCCCGGCCAGCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 945 − 0 37 1 0 CGCGAATGCCCGGACACGGAAGGAGCCTGGCTT 1801 CCCGCCCACCATACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 980 + 0 37 1 0 CGCGAATGCCCGCCTCCAGTGACGGCGAAGGCA 1802 CCATGTCCCGCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1015 − 0 37 1 0 CGCGAATGCCGCTGGGACTCACAGGGCTGCCGT 1803 CCACCGAGGCTGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1050 + 0 37 1 0 CGCGAATGCCACCAACAGAACCCACGCCCACCG 1804 GCATCGGGGCAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1085 − 0 37 1 0 CGCGAATGCCAGCGGCTGTGGTTGAGCGGGGGG 1805 TGCAGCCGGGCGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1120 + 0 37 1 0 CGCGAATGCCCCATCCCCATGCCGGCTTCCCGCT 1806 GCTCGCCTTCGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1155 − 0 37 1 0 CGCGAATGCCGTGCTACTGGACGACAGACTGAC 1807 CGGGCTGGTGGCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1190 + 0 37 1 0 CGCGAATGCCCAGTGGCCATGGCTCCACCTCGG 1808 ATTGTCTCTTCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1225 − 0 37 1 0 CGCGAATGCCGGGGGAACCAGACACCGAAGCA 1809 CTAGATCGCCGTGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1260 + 0 37 1 0 CGCGAATGCCAGCGATGGCGGTTTCATCTCCTC 1810 GGATGAGTATGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1295 − 0 37 1 0 CGCGAATGCCTGCGGAAGGAACTCCGGAAATCG 1811 CAGGGACTGGAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1330 + 0 37 1 0 CGCGAATGCCGTGTCACTCCGGATTCCCTGGGC 1812 CACACCCCACCAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1365 − 0 37 1 0 CGCGAATGCCCAGATATAGTTGCTTAGCTCCTCC 1813 TCACCGCGGGCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1400 + 0 37 1 0 CGCGAATGCCCATGGGTGGCAAGGGGCCCTCCA 1814 CCCTGACCGCCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1435 − 0 37 1 0 CGCGAATGCCATTGCCACCCCGAGACAAAATGT 1815 AGTGACCGTTGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1470 + 0 37 1 0 CGCGAATGCCGGCCACCGCTGCACCCCAGGAAC 1816 AGGCTTGGGCACACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1505 − 0 37 1 0 CGCGAATGCCCACTGGCTGCTTCATCCCCAGCC 1817 AAGGCTGGACTCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1540 + 0 37 1 0 CGCGAATGCCCTGCAGATCTGGATAATCGGTTC 1818 CGAAAGAGAACTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1575 − 0 37 1 0 CGCGAATGCCTGGTGGGTAATGGTAGGGGATGT 1819 GCCTGCCGAGTGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1610 + 0 37 1 0 CGCGAATGCCGAAGACCCCGTCCCAGTCCTCAG 1820 TGGCTTCCATTGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1645 − 0 37 1 0 CGCGAATGCCTGGTGGGTAGGCAGGCATCATCT 1821 CTGTGTACTCCTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1680 + 0 37 1 0 CGCGAATGCCGGAGGTGGCAGTGGAGGCCGACT 1822 GCCGGGACACAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1715 − 0 37 1 0 CGCGAATGCCCTGGGTAGGAGCGGGTGGGCACG 1823 AAGGCGGAGTGCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1750 + 0 37 1 0 CGCGAATGCCAGGAGGGTCTGGAAATGCACCCC 1824 TTGGAGCGTCGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1785 − 0 37 1 0 CGCGAATGCCTGGAGGGTGGAGCTGTCTGGGCG 1825 GTGGTGCCCCCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1820 + 0 37 1 0 CGCGAATGCCCACGGATGATGGCTACATGCCCA 1826 TGTCCCCAGGGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1855 − 0 37 1 0 CGCGAATGCCTCCACTGCCCTTTCGGCCACTGG 1827 GCACTGGGGCCAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1890 + 0 37 1 0 CGCGAATGCCGACTATATGCCCATGAGCCCCAA 1828 GAGCGTATCTGCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1925 − 0 37 1 0 CGCGAATGCCGATGGCGTCTGATGGGATTGATG 1829 ATCTGCTGTGGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1960 + 0 37 1 0 CGCGAATGCCCCCAGAGAGTGGACCCCAATGGC 1830 TACATGATGATGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 1995 − 0 37 1 0 CGCGAATGCCCCTCCAATGTCAGGAGAGCAGCC 1831 ACCGCTGGGGGAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2030 + 23 37 1 0 CGCGAATGCCTGGCCCCAGCAGCAGCAGCAGCA 1832 GCAGCAACGCCGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2065 − 0 37 1 0 CGCGAATGCCTGTCCACAGCTTTCCATAGCTGGT 1833 CCCGGAAGGGAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2100 + 0 37 1 0 CGCGAATGCCAACGGGGTAGGGGGCCACCACTC 1834 TCATGTCTTGCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2135 − 0 37 1 0 CGCGAATGCCTACCACCGCTGCTCTCCACTGGG 1835 GGTTTGGGGTGAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2170 + 0 37 1 0 CGCGAATGCCAGCTCTTACCTTGCACAGGTGAC 1836 TACATGAACATGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2205 − 0 37 1 0 CGCGAATGCCGAGGGGCTGCTGGTGTTGGAGTC 1837 CCCCACTGGTGAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2240 + 0 37 1 0 CGCGAATGCCCGACTGCTACTACGGCCCTGAGG 1838 ACCCCCAGCACAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2275 − 0 37 1 0 CGCGAATGCCGGATCTTGGCAATGAGTAGTAGG 1839 AGAGGACTGGCTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2310 + 0 37 1 0 CGCGAATGCCTTTAAGCACACCCAGCGCCCCGG 1840 GGAGCCGGAGGAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2345 − 0 37 1 0 CGCGAATGCCTAGTGGAAAGGCGGAGGTGCTGA 1841 TGCCGGGCACCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2380 + 0 37 1 0 CGCGAATGCCGCTCTGGTCGCCTTCTCTATGCTG 1842 CAACAGCAGATACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2415 − 0 37 1 0 CGCGAATGCCCCCAGGCTGTCGCTGCTGGTGGA 1843 AGAGGAAGAATCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2450 + 0 37 1 0 CGCGAATGCCTGGGGGATACTGCGGGGCTAGGC 1844 TGGAGCCCAGCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2485 − 0 37 1 0 CGCGAATGCCATGGGGCTGCAGAACCTGATGGT 1845 GGGGATGTGGAAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2520 + 0 37 1 0 CGCGAATGCCCTGCCTCGAAAGGTGGACACAGC 1846 TGCTCAGACCAAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2555 − 0 37 1 0 CGCGAATGCCCCAGGGACAGCCTCGTGGGCCGG 1847 GCCAGGCGGCTAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2590 + 0 37 1 0 CGCGAATGCCGGGATCCCAAGGCCAGCACCTTA 1848 CCTCGGGCCCGAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2629 − 18 37 1 0 CGCGAATGCCTGGAGGGTGCAGCAAGGGCTGCT 1849 GCTGCTGCTGCTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2660 + 0 37 1 0 CGCGAATGCCTCCAGAGCCCAAGAGCCCGGGGG 1850 AATATGTCAATAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2695 − 0 37 1 0 CGCGAATGCCAGACAAGTAGCCAGACTGATCAC 1851 TCCCAAATTCAAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2730 + 0 37 1 0 CGCGAATGCCGGCCCGGTGGCTTTCCACAGCTC 1852 ACCTTCTGTCAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2765 − 0 37 1 0 CGCGAATGCCCCTCTCTGGGAGCTGGCTGGAGC 1853 TGGGATGGACACACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2800 + 0 37 1 0 CGCGAATGCCAAGAGACTGGCACTGAGGAGTAC 1854 ATGAAGATGGACACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2835 − 0 37 1 0 CGCGAATGCCCTCTCCTGCCAGGCTGCCCTCCG 1855 GCCCGGCCCCAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2870 + 0 37 1 0 CGCGAATGCCCACTGGGGTCGAGATGGGCAGAC 1856 TGGGCCCTGCACACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2905 − 0 37 1 0 CGCGAATGCCCCGGGTAGGCCTGCAAATGCTAG 1857 CAGCCCCGGGAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2940 + 0 37 1 0 CGCGAATGCCGCAGTGCCCAGCAGCCGGGGTGA 1858 CTACATGACCATACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 2975 − 0 37 1 0 CGCGAATGCCTGTCCACGTAGCTCTGACGGGGA 1859 CAACTCATCTGCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3010 + 0 37 1 0 CGCGAATGCCCCTCGCCAGCTGCCCCTGTAAGC 1860 TATGCTGACATGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3045 − 0 37 1 0 CGCGAATGCCGGCAGGCTCACCTCCTCTGCAGC 1861 AATGCCTGTTCGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3080 + 0 37 1 0 CGCGAATGCCCAGGGCCACCATGGCTGCTGCCT 1862 CCTCATCCTCAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3115 − 0 37 1 0 CGCGAATGCCTGCCCCTTGAGGCCCAGTCGGGG 1863 AAGCAGAGGCTGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3150 + 0 37 1 0 CGCGAATGCCGCAGAGCTGGCTGCCCACTCGTC 1864 CCTGCTGGGGGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3185 − 0 37 1 0 CGCGAATGCCGGGTGAAGGCGCTCATGCCCCCA 1865 GGTCCTTGTGGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3220 + 0 37 1 0 CGCGAATGCCGGGTGAACCTCAGTCCTAACCGC 1866 AACCAGAGTGCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3255 − 0 37 1 0 CGCGAATGCCCGCCGGCACCCTTGTGGGTCTGC 1867 ACGGATCACTTTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3290 + 0 37 1 0 CGCGAATGCCGAGGCATAGCTCCGAGACTTTCT 1868 CCTCAACACCCAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3325 − 0 37 1 0 CGCGAATGCCTCCAAAGGGCACTGTGTTGCCCA 1869 CCCGGGTGGCACACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3360 + 0 37 1 0 CGCGAATGCCGCGGGGGCAGCAGTAGGGGGCG 1870 GTGGCGGTAGCAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3395 − 0 37 1 0 CGCGAATGCCCAGAGCTGTGGCGTTTCACATCC 1871 TCGCTGCTGCTGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3430 + 0 37 1 0 CGCGAATGCCCTTTCCTTTGAGAATGTGTGGCTG 1872 AGGCCTGGGGAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3465 − 0 37 1 0 CGCGAATGCCCACAGTTTGGCTGGCTCCTTGGG 1873 GGCTCCCCCAAGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3500 + 0 37 1 0 CGCGAATGCCTGGGGCTGCTGGGGGTTTGGAGA 1874 ATGGTCTTAACTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3535 − 0 37 1 0 CGCGAATGCCCTGTTTGAAGTCCTTGACCAAAT 1875 CCAGGTCTATGTACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3570 + 0 37 1 0 CGCGAATGCCTGCCCTCAGGAGTGCACCCCTGA 1876 ACCGCAGCCTCCACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3605 − 0 37 1 0 CGCGAATGCCCGCTGCCCAGGGGTTGATGAGGG 1877 GGTGGGGGTGGGACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3640 + 0 37 1 0 CGCGAATGCCGTGAGAGCAGCTCCACCCGCCGC 1878 TCAAGTGAGGATACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3675 − 0 37 1 0 CGCGAATGCCTGCTTCTGGAAACTGATGCTGGC 1879 ATAGGCGCTTAAACGCGTGGCGGATGT IRS1 NM_005544 2 2 − 3710 + 0 37 1 0 CGCGAATGCCGCCAGAGGACCGTCAGTAGCTCA 1880 ACTGGACATCACACGCGTGGCGGATGT RBBP8 NM_002894 3 18 + −48 + 0 37 1 0 CGCGAATGCCCATAAAGGAACTGTTGTAGAAGT 1881 AATACCTTTTCTACGCGTGGCGGATGT RBBP8 NM_002894 3 18 + −13 − 0 37 1 0 CGCGAATGCCTTGGTTACTTTTACTTGTAAACCT 1882 GAAAAGTAAAAACGCGTGGCGGATGT RBBP8 NM_002894 3 18 + 22 + 0 37 1 0 CGCGAATGCCGCTAAAACAGGAACGAATCTTGT 1883 AAGTATCAGTATACGCGTGGCGGATGT RBBP8 NM_002894 3 18 + 57 − 0 37 1 0 CGCGAATGCCTATCAACTACTCTATAAATAACA 1884 CATGAGTATTACACGCGTGGCGGATGT NTRK3 NM_001012338 11 15 − 0 + 0 37 1 0 CGCGAATGCCATCCCCCACGTGTGGTGAGCCTG 1885 GAGGAGCCTGAGACGCGTGGCGGATGT NTRK3 NM_001012338 11 15 − 35 − 0 37 1 0 CGCGAATGCCCGCACCACAAACTCGATGCAGTG 1886 CTCCAGGCGCAGACGCGTGGCGGATGT NTRK3 NM_001012338 11 15 − 70 + 0 37 1 0 CGCGAATGCCTGGCAACCCCCCACCAACGCTGC 1887 ACTGGCTGCACAACGCGTGGCGGATGT NTRK3 NM_001012338 11 15 − 105 − 0 37 1 0 CGCGAATGCCATGGATGATCTTGGACTCCCGCA 1888 GAGGCTGCCCATACGCGTGGCGGATGT NTRK3 NM_001012338 11 15 − 140 + 0 37 1 0 CGCGAATGCCGTGGAATACTACCAAGAGGGAG 1889 AGATTTCCGAGGGACGCGTGGCGGATGT NTRK3 NM_001012338 11 15 − 175 − 0 37 1 0 CGCGAATGCCTGTTGTAGTGGGTGGGCTTGTTG 1890 AAGAGCAGGCAGACGCGTGGCGGATGT NTRK3 NM_001012338 11 15 − 210 + 0 37 1 0 CGCGAATGCCATGGCAACTATACCCTCATTGCC 1891 AAAAACCCACTGACGCGTGGCGGATGT NTRK3 NM_001012338 11 15 − 245 − 0 37 1 0 CGCGAATGCCAGGAAGTGGCCATTGATGGTCTG 1892 GTTGGCTGTGCCACGCGTGGCGGATGT NTRK3 NM_001012338 11 15 − 280 + 0 37 1 0 CGCGAATGCCCAAGGAGCCCTTTCCAGGTGAGG 1893 GCAGCGTAGCTGACGCGTGGCGGATGT PDGFRA NM_006206 21 4 + 0 + 0 37 1 0 CGCGAATGCCGTGGCACCCCTTACCCCGGCATG 1894 ATGGTGGATTCTACGCGTGGCGGATGT PDGFRA NM_006206 21 4 + 35 − 0 37 1 0 CGCGAATGCCATCCGGTACCCACTCTTGATCTTA 1895 TTGTAGAAAGTACGCGTGGCGGATGT PDGFRA NM_006206 21 4 + 70 + 0 37 1 0 CGCGAATGCCGGCCAAGCCTGACCACGCTACCA 1896 GTGAAGTGTGAGACGCGTGGCGGATGT PDGFRA NM_006206 21 4 + 105 − 0 37 1 0 CGCGAATGCCGACTGTGAACACAGGCCCCCGGG 1897 ATGGGGAAGGAGACGCGTGGCGGATGT PDGFRA NM_006206 21 4 + 140 + 0 37 1 0 CGCGAATGCCTGTGGGTCTAGGGGGAGGGAGG 1898 GGCCCTGAGACTTACGCGTGGCGGATGT PDGFRA NM_006206 21 4 + 175 − 0 37 1 0 CGCGAATGCCCTGTGGGGACAGAACTCAAGAGT 1899 GGGCACAGGGGGACGCGTGGCGGATGT PDGFRA NM_006206 21 4 + 210 + 0 37 1 0 CGCGAATGCCCTACGAGATCATGGTGAAATGCT 1900 GGAACAGTGAGCACGCGTGGCGGATGT PDGFRA NM_006206 21 4 + 245 − 0 37 1 0 CGCGAATGCCCTCACTCAGGTGGTAAAAGGAGG 1901 GTCTCTTCTCCGACGCGTGGCGGATGT PDGFRA NM_006206 21 4 + 280 + 0 37 1 0 CGCGAATGCCATTGTGGAGAATCTGCTGCCTGG 1902 ACAATATAAAAAACGCGTGGCGGATGT PDGFRA NM_006206 21 4 + 315 − 0 37 1 0 CGCGAATGCCATCCAGACCTTTCCACCCACAGA 1903 TCCAAACACACCACGCGTGGCGGATGT PDGFRA NM_006206 9 4 + −6 + 0 37 1 0 CGCGAATGCCTTGCAGTTCCTTCATCCATTCTGG 1904 ACTTGGTCGATACGCGTGGCGGATGT PDGFRA NM_006206 9 4 + 29 − 0 37 1 0 CGCGAATGCCCTCACCGTCTGTCCCCCAGTTGA 1905 GCCATGGTGATCACGCGTGGCGGATGT PDGFRA NM_006206 9 4 + 64 + 0 37 1 0 CGCGAATGCCGTGCACAGCTGAAGGCACGCCGC 1906 TTCCTGATATTGACGCGTGGCGGATGT PDGFRA NM_006206 9 4 + 99 − 0 37 1 0 CGCGAATGCCTCCATACTTCTTAATATCTTTGCA 1907 TATCATCCACTACGCGTGGCGGATGT PDGFRA NM_006206 13 4 + −18 + 0 37 1 0 CGCGAATGCCGATTCTGCCTGCCCACAGGTCGG 1908 GTCTTGGGGTCTACGCGTGGCGGATGT PDGFRA NM_006206 13 4 + 17 − 0 37 1 0 CGCGAATGCCTAGGCTGTTCCTTCAACCACCTTC 1909 CCAAACGCTCCACGCGTGGCGGATGT PDGFRA NM_006206 13 4 + 52 + 0 37 1 0 CGCGAATGCCTGGATTAAGCCGGTCCCAACCTG 1910 TCATGAAAGTTGACGCGTGGCGGATGT PDGFRA NM_006206 13 4 + 87 − 0 37 1 0 CGCGAATGCCCAGGAAGGAGCACTTACGTTTTA 1911 GCATCTTCACTGACGCGTGGCGGATGT GUCY2F NM_001522 13 X − −25 + 0 37 1 0 CGCGAATGCCTTCATTCTCCTCCTTTTCTTTCTAG 1912 GGTGATTGGGACGCGTGGCGGATGT GUCY2F NM_001522 13 X − 10 − 0 37 1 0 CGCGAATGCCTCCAAAATCTCCAAGGGAGAACT 1913 TTTTCAGCCACAACGCGTGGCGGATGT GUCY2F NM_001522 13 X − 45 + 0 37 1 0 CGCGAATGCCGACCTTAAGTCCATCAAATCAAG 1914 AGCAAGTGATGTACGCGTGGCGGATGT GUCY2F NM_001522 13 X − 80 − 0 37 1 0 CGCGAATGCCTTACATAAACGAAGATTTCTCAT 1915 ACCATTTCGAACACGCGTGGCGGATGT NTRK3 NM_001012338 17 15 − −32 + 0 37 1 0 CGCGAATGCCGGCTGACCGTTACCTTTCCTCCTC 1916 CCCTGCAGACAACGCGTGGCGGATGT NTRK3 NM_001012338 17 15 − 3 − 0 37 1 0 CGCGAATGCCCGTTGAGCGTGTGAAGACTGCGC 1917 CAGTTCTCTATGACGCGTGGCGGATGT NTRK3 NM_001012338 17 15 − 38 + 0 37 1 0 CGCGAATGCCCCGTGGACATGGAGCTCTACACC 1918 GGACTTCAAAAGACGCGTGGCGGATGT NTRK3 NM_001012338 17 15 − 73 − 0 37 1 0 CGCGAATGCCGGGAAAGGCCTCTCTGTGGCCGG 1919 GTGTACTCACAGACGCGTGGCGGATGT CHAF1A NM_005483 2 19 + −44 + 0 37 1 0 CGCGAATGCCTTTGCAGAATTTAACAGTTTACCC 1920 TTTGACACTTTACGCGTGGCGGATGT CHAF1A NM_005483 2 19 + −9 − 0 37 1 0 CGCGAATGCCAGCTGGTCTATCTTTGCAATCCAT 1921 GGCTGCAAATAACGCGTGGCGGATGT CHAF1A NM_005483 2 19 + 26 + 0 37 1 0 CGCGAATGCCTTTCCAGTTAAGAAGTTAATACA 1922 AGGTAATTATTTACGCGTGGCGGATGT CHAF1A NM_005483 2 19 + 61 − 0 37 1 0 CGCGAATGCCACTATAAACTACGAACAACTCTT 1923 TAACCCATTTCCACGCGTGGCGGATGT PKN1 NM_002741 5 19 + 0 + 0 37 1 0 CGCGAATGCCGGAGTCCTGACCTGGGGGCTGTG 1924 GAGCTGCGCATCACGCGTGGCGGATGT PKN1 NM_002741 5 19 + 35 − 0 37 1 0 CGCGAATGCCGCGTGCTCCACTCGGAAGTGGTG 1925 CCGCAGCTCTTCACGCGTGGCGGATGT PKN1 NM_002741 5 19 + 70 + 0 37 1 0 CGCGAATGCCGGTGGCCGAGGGTGCCAAGAAC 1926 GTACTGCGCCTGCACGCGTGGCGGATGT PKN1 NM_002741 5 19 + 105 − 0 37 1 0 CGCGAATGCCGACTGCCTTGCGGTCCGGGGCCT 1927 TGGCAGCGCTGAACGCGTGGCGGATGT PKN1 NM_002741 5 19 + 140 + 0 37 1 0 CGCGAATGCCAGCGAGGTGAGGGGCGGAGCTTT 1928 CATTAGAGGCGGACGCGTGGCGGATGT KSR2 NM_173598 20 12 − −24 + 0 37 1 0 CGCGAATGCCCAGCAGTGCGAACTGGTCCAAAA 1929 CATGATAGACTTACGCGTGGCGGATGT KSR2 NM_173598 20 12 − 11 − 0 37 1 0 CGCGAATGCCATTTGGTCCTAAGCCCTTCCAGGT 1930 TGGAGATGCTCACGCGTGGCGGATGT KSR2 NM_173598 20 12 − 46 + 0 37 1 0 CGCGAATGCCGTGCTACCTCCAACGACCTCACA 1931 CAAAAAGAAATCACGCGTGGCGGATGT KSR2 NM_173598 20 12 − 77 − 1 37 1 0 CGCGAATGCCTCCCGTAGGCAACACCTACCTCC 1932 AGGGTCCGGATTACGCGTGGCGGATGT EPHA7 NM_004440 17 6 − −22 + 0 37 1 0 CGCGAATGCCAAATAAAACCTGCTCATGCACCA 1933 TGGTTTTTCAAAACGCGTGGCGGATGT EPHA7 NM_004440 17 6 − 13 − 0 37 1 0 CGCGAATGCCGATGTAGCATAAAATAATCCATG 1934 AAGGGTACCGAGACGCGTGGCGGATGT EPHA7 NM_004440 17 6 − 48 + 0 37 1 0 CGCGAATGCCTGGCTGCTCCGCTTTGCACACAC 1935 AGGGGAGGCGCAACGCGTGGCGGATGT EPHA7 NM_004440 17 6 − 83 − 0 37 1 0 CGCGAATGCCTTTGCTTTCCCATCACCTTACCTT 1936 CCTTCGCAGCCACGCGTGGCGGATGT NFKB1 NM_003998 16 4 + −12 + 0 37 1 0 CGCGAATGCCTTTTCATTCCAGTGTCTTACACTT 1937 AGCAATCATCCACGCGTGGCGGATGT NFKB1 NM_003998 16 4 + 23 − 0 37 1 0 CGCGAATGCCTTCTAGTAGATCCCTCACAAGTT 1938 GAGAATGAAGGTACGCGTGGCGGATGT NFKB1 NM_003998 16 4 + 58 + 0 37 1 0 CGCGAATGCCGTCACATCTGGTTTGATTTCTGAT 1939 GACATTATCAAACGCGTGGCGGATGT NFKB1 NM_003998 16 4 + 93 − 0 37 1 0 CGCGAATGCCGATTTCTGCTTACCTGGTACAGAT 1940 CATTTCTCATGACGCGTGGCGGATGT EPHA4 NM_004438 15 2 − 0 + 0 37 1 0 CGCGAATGCCCTTGCAAAATTGGATATTACAAG 1941 GCTCTCTCCACGACGCGTGGCGGATGT EPHA4 NM_004438 15 2 − 35 − 0 37 1 0 CGCGAATGCCTAGCTGTGGGGTGGGCACTTGGC 1942 ACAGGTGGCATCACGCGTGGCGGATGT EPHA4 NM_004438 15 2 − 70 + 0 37 1 0 CGCGAATGCCCTCTGTCTGGGAAGGAGCCACCT 1943 CGTGCACCTGTGACGCGTGGCGGATGT EPHA4 NM_004438 15 2 − 105 − 0 37 1 0 CGCGAATGCCGGCAGCATCGTTGTCAGCTCTGA 1944 AAAAGCCTCGGTACGCGTGGCGGATGT EPHA4 NM_004438 15 2 − 140 + 0 37 1 0 CGCGAATGCCTCTATGCCCTGCACCCGTAAGTT 1945 GTATGCTTGTCTACGCGTGGCGGATGT RB1 NM_000321 11 13 + −31 + 0 37 1 0 CGCGAATGCCTATGTGAATGACTTCACTTATTGT 1946 TATTTAGTTTTACGCGTGGCGGATGT RB1 NM_000321 11 13 + 4 − 0 37 1 0 CGCGAATGCCTCAAGGTTACTTTTTCGTGGTGTT 1947 CTCTGTGTTTCACGCGTGGCGGATGT RB1 NM_000321 11 13 + 39 + 0 37 1 0 CGCGAATGCCTGAAGAGGTGAATGTAATTCCTC 1948 CACACACTCCAGACGCGTGGCGGATGT RB1 NM_000321 11 13 + 70 − 11 37 1 0 CGCGAATGCCTATAATTAAAAGTAGGAAAATTC 1949 ATACCTAACTGGACGCGTGGCGGATGT EPHA4 NM_004438 8 2 − 0 + 0 37 1 0 CGCGAATGCCGTGAATTTGGTGAGGTATGCAGT 1950 GGGCGTCTCAAAACGCGTGGCGGATGT EPHA4 NM_004438 8 2 − 35 − 0 37 1 0 CGCGAATGCCTTGATAGCCACACAGATCTCTCT 1951 CTTGCCAGGCACACGCGTGGCGGATGT EPHA4 NM_004438 8 2 − 70 + 0 37 1 0 CGCGAATGCCGACTCTGAAAGCTGGTTATACAG 1952 ACAAACAGAGGAACGCGTGGCGGATGT EPHA4 NM_004438 8 2 − 105 − 0 37 1 0 CGCGAATGCCCTGTCCCATGATGCTGGCCTCACT 1953 CAGGAAGTCTCACGCGTGGCGGATGT EPHA4 NM_004438 8 2 − 140 + 0 37 1 0 CGCGAATGCCTTTGACCATCCGAACATCATTCA 1954 CTTGGAAGGCGTACGCGTGGCGGATGT EPHA4 NM_004438 8 2 − 175 − 0 37 1 0 CGCGAATGCCTTGTAAAGGGGGTGACCCACGTA 1955 CATTTAGTGACCACGCGTGGCGGATGT RB1 NM_000321 14 13 + −42 + 0 37 1 0 CGCGAATGCCCTAAAATAGCAGGCTCTTATTTTT 1956 CTTTTTGTTTGACGCGTGGCGGATGT RB1 NM_000321 14 13 + −7 − 0 37 1 0 CGCGAATGCCAATACAAGCGAACTCCAAGTTTG 1957 TATCGCTACAAAACGCGTGGCGGATGT RB1 NM_000321 14 13 + 28 + 0 37 1 0 CGCGAATGCCACCGAGTAATGGAATCCATGCTT 1958 AAATCAGTAAGTACGCGTGGCGGATGT RB1 NM_000321 14 13 + 59 − 8 37 1 0 CGCGAATGCCGCCCGGCTGAAATTTTTTTATATT 1959 GTTTTTAACTTACGCGTGGCGGATGT PALB2 NM_024675 13 16 − −46 + 0 37 1 0 CGCGAATGCCGCCTGGGGTCGGCGACGGCTGCT 1960 CTTTTCGTTCTGACGCGTGGCGGATGT PALB2 NM_024675 13 16 − −11 − 0 37 1 0 CGCGAATGCCGGGCTTCCCGGGAGGCTCGTCCA 1961 TCGGGCAGGCGAACGCGTGGCGGATGT PALB2 NM_024675 13 16 − 24 + 0 37 1 0 CGCGAATGCCCTCAGCTGTGAGGAGAAGGAAA 1962 AGGTGCCGGGGGTACGCGTGGCGGATGT PALB2 NM_024675 13 16 − 59 − 0 37 1 0 CGCGAATGCCAAGCGGGGTCAGAGTCCTGCGTC 1963 CGCCCTTCCCGCACGCGTGGCGGATGT EPHA3 NM_005233 12 3 + −39 + 0 37 1 0 CGCGAATGCCACTGTACTGATTATTATTTATTAT 1964 TTACTGTATATACGCGTGGCGGATGT EPHA3 NM_005233 12 3 + −4 − 0 37 1 0 CGCGAATGCCATGTATTCTGTGACAATCATAAC 1965 TGGCTTACCTAGACGCGTGGCGGATGT EPHA3 NM_005233 12 3 + 31 + 0 37 1 0 CGCGAATGCCGGAGAATGGTTCCTTGGATAGTT 1966 TCCTACGTGTAAACGCGTGGCGGATGT EPHA3 NM_005233 12 3 + 66 − 0 37 1 0 CGCGAATGCCATTTATTCATATATATGTATGTGT 1967 GTGCATCTTACACGCGTGGCGGATGT RB1 NM_000321 13 13 + −12 + 0 37 1 0 CGCGAATGCCTACCTCCTAAAGAACTGCACAGT 1968 GAATCCAAAAGAACGCGTGGCGGATGT RB1 NM_000321 13 13 + 23 − 0 37 1 0 CGCGAATGCCTGTATCCTATATCCTTCACTCTTT 1969 TCAGTATACTTACGCGTGGCGGATGT RB1 NM_000321 13 13 + 58 + 0 37 1 0 CGCGAATGCCTCTTTAAAGAGAAATTTGCTAAA 1970 GCTGTGGGACAGACGCGTGGCGGATGT RB1 NM_000321 13 13 + 93 − 0 37 1 0 CGCGAATGCCATTCAAGTTACCTGTGATCCAATT 1971 TCGACACAACCACGCGTGGCGGATGT RPS6KA1 NM_002953 2 1 + −48 + 0 37 1 0 CGCGAATGCCGCGTGTAAGTTCTGACAGTGCTC 1972 CCCCAATCTCCTACGCGTGGCGGATGT RPS6KA1 NM_002953 2 1 + −13 − 0 37 1 0 CGCGAATGCCCTTCCCCTGAGGTCTGTCCATTCT 1973 GGAAAAGAGAAACGCGTGGCGGATGT RPS6KA1 NM_002953 2 1 + 22 + 0 37 1 0 CGCGAATGCCAAGCTGGACTTCAGCCGTCCAAG 1974 GTGAGGACCATGACGCGTGGCGGATGT RPS6KA1 NM_002953 2 1 + 57 − 0 37 1 0 CGCGAATGCCAGGATCCCCCACAGCCCCTCGCT 1975 CAGGGTGCTGGCACGCGTGGCGGATGT RB1 NM_000321 6 13 + −36 + 0 37 1 0 CGCGAATGCCTTCCTGTTTTTTTTCTGCTTTCTAT 1976 TTGTTTAATAACGCGTGGCGGATGT RB1 NM_000321 6 13 + −1 − 0 37 1 0 CGCGAATGCCTAGCACCAATGCAGAATTTATTT 1977 CAGTAGATATCCACGCGTGGCGGATGT RB1 NM_000321 6 13 + 34 + 0 37 1 0 CGCGAATGCCAAAGTTTCTTGGATCACATTTTTA 1978 TTAGCTAAAGGACGCGTGGCGGATGT RB1 NM_000321 6 13 + 69 − 0 37 1 0 CGCGAATGCCTGAAATATTAGCATTTAATAAAT 1979 ATAATGAACTTAACGCGTGGCGGATGT CHAF1A NM_005483 15 19 + −20 + 0 37 1 0 CGCGAATGCCCTCCCTCTCTTGCCCTGCAGAGGT 1980 CCAAGCCCCGTACGCGTGGCGGATGT CHAF1A NM_005483 15 19 + 15 − 0 37 1 0 CGCGAATGCCCCCCACACCACCCCCAGCTCCGG 1981 AAGCGGCTCCACACGCGTGGCGGATGT CHAF1A NM_005483 15 19 + 50 + 0 37 1 0 CGCGAATGCCGTGGACACCGGCAAGGCCACCCT 1982 GACCTCGAGCCCACGCGTGGCGGATGT CHAF1A NM_005483 15 19 + 85 − 0 37 1 0 CGCGAATGCCACATACGTCACCCCTGCTCTCAG 1983 GATGCACCCAGTACGCGTGGCGGATGT RET NM_020630 18 10 + −20 + 0 37 1 0 CGCGAATGCCCTGTCTGCTCTTCCCACCAGGTAC 1984 CGCCTGATGCTACGCGTGGCGGATGT RET NM_020630 18 10 + 15 − 0 37 1 0 CGCGAATGCCCCGGCCTTTTGTCCGGCTCCTGCT 1985 TCCAGCATTGCACGCGTGGCGGATGT RET NM_020630 18 10 + 50 + 0 37 1 0 CGCGAATGCCTGTTTGCGGACATCAGCAAAGAC 1986 CTGGAGAAGATGACGCGTGGCGGATGT RET NM_020630 18 10 + 85 − 0 37 1 0 CGCGAATGCCAATTGGACCCAGGCACTCACTCT 1987 CCTCTTAACCATACGCGTGGCGGATGT NFKB1 NM_003998 17 4 + 0 + 0 37 1 0 CGCGAATGCCACGCCCTTGCACTTGGCAGTGAT 1988 CACTAAGCAGGAACGCGTGGCGGATGT NFKB1 NM_003998 17 4 + 35 − 0 37 1 0 CGCGAATGCCCGGCCCCAGCCCTCAGCAAATCC 1989 TCCACCACATCTACGCGTGGCGGATGT NFKB1 NM_003998 17 4 + 70 + 0 37 1 0 CGCGAATGCCACCTGAGCCTTCTGGACCGCTTG 1990 GGTAACTCTGTTACGCGTGGCGGATGT NFKB1 NM_003998 17 4 + 105 − 0 37 1 0 CGCGAATGCCACTTTATCATGTCCTTCTTTGGCA 1991 GCTAGGTGCAAACGCGTGGCGGATGT NFKB1 NM_003998 17 4 + 140 + 0 37 1 0 CGCGAATGCCTCTCAGTATCTTACTCAAGCACA 1992 AAAAGGCAGCACACGCGTGGCGGATGT NFKB1 NM_003998 17 4 + 175 − 0 37 1 0 CGCGAATGCCTCTCTTACCGTCCCCGTTGGGGTG 1993 GTCAAGAAGTAACGCGTGGCGGATGT NFKB1 NM_003998 5 4 + −20 + 0 37 1 0 CGCGAATGCCCTTAACGTTCACCTTTGCAGAGA 1994 GGATTTCGTTTCACGCGTGGCGGATGT NFKB1 NM_003998 5 4 + 15 − 0 37 1 0 CGCGAATGCCAGTCCACCATGGGATGGGCCTTC 1995 ACATACATAACGACGCGTGGCGGATGT NFKB1 NM_003998 5 4 + 50 + 0 37 1 0 CGCGAATGCCACCTGGTGCCTCTAGTGAAAAGA 1996 ACAAGAAGTCTTACGCGTGGCGGATGT NFKB1 NM_003998 5 4 + 85 − 0 37 1 0 CGCGAATGCCGGAGAGCTACCACAAACTTACTT 1997 TGACCTGAGGGTACGCGTGGCGGATGT EPHB1 NM_004441 11 3 + 0 + 0 37 1 0 CGCGAATGCCGGGAGTTTGGAGAAGTGTACAAG 1998 GGGCGTTTGAAAACGCGTGGCGGATGT EPHB1 NM_004441 11 3 + 35 − 0 37 1 0 CGCGAATGCCTTGATGGCCACGTAGATTTCCCTC 1999 TTGCCTGGCAGACGCGTGGCGGATGT EPHB1 NM_004441 11 3 + 70 + 0 37 1 0 CGCGAATGCCGACCCTGAAGGCAGGGTACTCGG 2000 AGAAGCAGCGTCACGCGTGGCGGATGT EPHB1 NM_004441 11 3 + 105 − 0 37 1 0 CGCGAATGCCCTGGCCCATGATGCTCGCCTCAC 2001 TCAGAAAGTCCCACGCGTGGCGGATGT EPHB1 NM_004441 11 3 + 140 + 0 37 1 0 CGCGAATGCCTTCGACCATCCTAACATCATTCGC 2002 CTGGAGGGTGTACGCGTGGCGGATGT EPHB1 NM_004441 11 3 + 175 − 0 37 1 0 CGCGAATGCCCTGTGATGATCATGACAGGCCGA 2003 CTCTTGGTGACCACGCGTGGCGGATGT EPHB1 NM_004441 11 3 + 210 + 0 37 1 0 CGCGAATGCCAGTTCATGGAGAATGGTGCATTG 2004 GATTCTTTCCTCACGCGTGGCGGATGT EPHB1 NM_004441 11 3 + 245 − 0 37 1 0 CGCGAATGCCAGTGCATCATCAAACCCCTGAGT 2005 TGCTCTTACCCTACGCGTGGCGGATGT EPHB1 NM_004441 15 3 + 0 + 0 37 1 0 CGCGAATGCCGCCTTCCCAGCCCCTGCTCGACC 2006 GCTCCATCCCAGACGCGTGGCGGATGT EPHB1 NM_004441 15 3 + 35 − 0 37 1 0 CGCGAATGCCGAGCCAGTCATCCACGGTGGTAA 2007 AGGCCGTGAAGTACGCGTGGCGGATGT EPHB1 NM_004441 15 3 + 70 + 0 37 1 0 CGCGAATGCCAGCGCCATCAAAATGGTCCAGTA 2008 CAGGGACAGCTTACGCGTGGCGGATGT EPHB1 NM_004441 15 3 + 105 − 0 37 1 0 CGCGAATGCCTGACCAGCTGGAGGGAGGTGAA 2009 GCCAGCAGTGAGGACGCGTGGCGGATGT EPHB1 NM_004441 15 3 + 140 + 0 37 1 0 CGCGAATGCCCCCAGATGACATCAGAGTAAGTG 2010 ATGAGAATCTCTACGCGTGGCGGATGT PIK3CA NM_006218 6 3 + −27 + 0 37 1 0 CGCGAATGCCTAGTATATACCTACTTTTTTCTTT 2011 TAGATCTATGTACGCGTGGCGGATGT PIK3CA NM_006218 6 3 + 8 − 0 37 1 0 CGCGAATGCCATAAGGGTTCTCCTCCATGGTAG 2012 ATACCTGTTCGAACGCGTGGCGGATGT PIK3CA NM_006218 6 3 + 43 + 0 37 1 0 CGCGAATGCCGTGACAATGTGAACACTCAAAGA 2013 GTACCTTGTTCCACGCGTGGCGGATGT PIK3CA NM_006218 6 3 + 78 − 0 37 1 0 CGCGAATGCCGAAATATAAATCTATATACTTCC 2014 TTACCTGGGATTACGCGTGGCGGATGT NTRK3 NM_001012338 6 15 − −4 + 0 37 1 0 CGCGAATGCCACAGATGTGCAGCACATTAAGAG 2015 GAGAGACATCGTACGCGTGGCGGATGT NTRK3 NM_001012338 6 15 − 31 − 0 37 1 0 CGCGAATGCCTTCCAAAGGCTCCCTCACCCAGT 2016 TCTCGCTTCAGCACGCGTGGCGGATGT NTRK3 NM_001012338 6 15 − 66 + 0 37 1 0 CGCGAATGCCAGGTCTTCCTGGCCGAGTGCTAC 2017 AACCTCAGCCCGACGCGTGGCGGATGT NTRK3 NM_001012338 6 15 − 101 − 0 37 1 0 CGCGAATGCCTTTACCTTCACAGCCACAAGCAT 2018 CTTGTCCTTGGTACGCGTGGCGGATGT CHAF1A NM_005483 14 19 + −22 + 0 37 1 0 CGCGAATGCCGCCGTGTACCCTGTCTGTCCAGA 2019 TTGGTGCTGAAGACGCGTGGCGGATGT CHAF1A NM_005483 14 19 + 9 − 6 37 1 0 CGCGAATGCCTCCTCCGTGTCTGCCTGGAAGCC 2020 GTCCATGTCTTCACGCGTGGCGGATGT CHAF1A NM_005483 14 19 + 52 + 9 37 1 0 CGCGAATGCCAGGAGGAGGGCGACTGTATGATC 2021 GTGGATGTCCCGACGCGTGGCGGATGT CHAF1A NM_005483 14 19 + 83 − 0 37 1 0 CGCGAATGCCGCTATCTACAGCCCTTCTCACCCG 2022 CAGCATCCGGGACGCGTGGCGGATGT NFKB1 NM_003998 8 4 + 0 + 0 37 1 0 CGCGAATGCCATCGGGAAAAAGAGCTAATCCGC 2023 CAAGCAGCTCTGACGCGTGGCGGATGT NFKB1 NM_003998 8 4 + 35 − 0 37 1 0 CGCGAATGCCCGCACCACGCTGAGGTCCATCTC 2024 CTTGGTCTGCTGACGCGTGGCGGATGT NFKB1 NM_003998 8 4 + 70 + 0 37 1 0 CGCGAATGCCGCTCATGTTTACAGCTTTTCTTCC 2025 GGATAGCACTGACGCGTGGCGGATGT NFKB1 NM_003998 8 4 + 105 − 0 37 1 0 CGCGAATGCCTGATACCACGGGTTCCAGGCGCC 2026 TTGTGAAGCTGCACGCGTGGCGGATGT NFKB1 NM_003998 8 4 + 140 + 0 37 1 0 CGCGAATGCCGACGCCATCTATGACAGTAGTGA 2027 GTACTTCACTTCACGCGTGGCGGATGT RPS6KA1 NM_002953 13 1 + −18 + 0 37 1 0 CGCGAATGCCGCCCACCCCACTGTGCAGAAGCT 2028 ATACCGTCGTGAACGCGTGGCGGATGT RPS6KA1 NM_002953 13 1 + 17 − 0 37 1 0 CGCGAATGCCGCTGAGCCACTGCTGGCTTGAAG 2029 GGTGGCTTGATCACGCGTGGCGGATGT RPS6KA1 NM_002953 13 1 + 52 + 0 37 1 0 CGCGAATGCCCTGATGACACCTTCTACTTTGACA 2030 CCGAGTTCACGACGCGTGGCGGATGT RPS6KA1 NM_002953 13 1 + 87 − 0 37 1 0 CGCGAATGCCAAACAGATAAGGGACGCACCCTT 2031 GGGTGTGCGGGAACGCGTGGCGGATGT EPHB1 NM_004441 14 3 + 0 + 0 37 1 0 CGCGAATGCCGTCATCAATGCCATCGAGCAGGA 2032 CTACCGGCTGCCACGCGTGGCGGATGT EPHB1 NM_004441 14 3 + 35 − 0 37 1 0 CGCGAATGCCGCTGGTGTAGAGCAGCTGGACAG 2033 TCCATGGGTGGGACGCGTGGCGGATGT EPHB1 NM_004441 14 3 + 70 + 0 37 1 0 CGCGAATGCCTCATGCTGGACTGTTGGCAGAAG 2034 GACCGGAACAGCACGCGTGGCGGATGT EPHB1 NM_004441 14 3 + 105 − 0 37 1 0 CGCGAATGCCTCTAGGGTGTTGACAATCTCCGC 2035 AAACCGGGGCCGACGCGTGGCGGATGT EPHB1 NM_004441 14 3 + 140 + 0 37 1 0 CGCGAATGCCTAAGATGATCCGGAACCCGGCAA 2036 GTCTCAAGACTGACGCGTGGCGGATGT EPHB1 NM_004441 14 3 + 175 − 0 37 1 0 CGCGAATGCCGTTTCACTAGACTCACACGGCGG 2037 TGATGGTTGCCAACGCGTGGCGGATGT NTRK3 NM_001012338 12 15 − 0 + 0 37 1 0 CGCGAATGCCACCAATCTGAACTGGACCAATGT 2038 TCATGCCATCAAACGCGTGGCGGATGT NTRK3 NM_001012338 12 15 − 35 − 0 37 1 0 CGCGAATGCCCATTGTCCTCACTCGTCACATTCA 2039 CCAGCGTCAAGACGCGTGGCGGATGT NTRK3 NM_001012338 12 15 − 70 + 0 37 1 0 CGCGAATGCCGCTTCACCCTGACGTGCATTGCA 2040 GAGAACGTGGTGACGCGTGGCGGATGT NTRK3 NM_001012338 12 15 − 105 − 0 37 1 0 CGCGAATGCCTAGACAGTGAGGGCAACACTGGC 2041 ATTGCTCATGCCACGCGTGGCGGATGT NTRK3 NM_001012338 12 15 − 140 + 0 37 1 0 CGCGAATGCCCTGTAAGTGCATGTTATTGTGGG 2042 GGATGGCTGTGTACGCGTGGCGGATGT EPHA7 NM_004440 4 6 − 0 + 0 37 1 0 CGCGAATGCCGGTGGAAAAATTCCAGTAAGGTG 2043 GACAGCACCCGAACGCGTGGCGGATGT EPHA7 NM_004440 4 6 − 35 − 0 37 1 0 CGCGAATGCCCACTGGCTGATGTGAATTTCCGG 2044 TACTGGATGGCTACGCGTGGCGGATGT EPHA7 NM_004440 4 6 − 70 + 0 37 1 0 CGCGAATGCCATGTATGGAGCTATGGAATAGTC 2045 ATGTGGGAAGTTACGCGTGGCGGATGT EPHA7 NM_004440 4 6 − 105 − 0 37 1 0 CGCGAATGCCGACATGTCCCAATAAGGTCTTTC 2046 TCCATAAGACATACGCGTGGCGGATGT EPHA7 NM_004440 4 6 − 140 + 0 37 1 0 CGCGAATGCCAAATCAAGATGTAGGTGTTACAT 2047 TCATTTAAACAAACGCGTGGCGGATGT EPHA7 NM_004440 14 6 − 0 + 0 37 1 0 CGCGAATGCCCCTGTGGCCGTGGGTTCTACAAG 2048 TCTTCCTCTCAAACGCGTGGCGGATGT EPHA7 NM_004440 14 6 − 35 − 0 37 1 0 CGCGAATGCCAAACTGTGAGTTGGACAACGAGA 2049 GCACTGAAGATCACGCGTGGCGGATGT EPHA7 NM_004440 14 6 − 70 + 0 37 1 0 CGCGAATGCCTTCTGATAAAGAAGGCTCCTCCA 2050 GATGTGAATGTGACGCGTGGCGGATGT EPHA7 NM_004440 14 6 − 105 − 0 37 1 0 CGCGAATGCCTGGTGGGTCAGATGGAGCCCTGT 2051 AATACCCATCTTACGCGTGGCGGATGT EPHA7 NM_004440 14 6 − 140 + 0 37 1 0 CGCGAATGCCTACGTTGCGTGCACAAGTGAGTT 2052 GTATTATGAAAGACGCGTGGCGGATGT NFKB1 NM_003998 12 4 + 0 + 0 37 1 0 CGCGAATGCCATAAAGAAGAAGTGCAGAGGAA 2053 ACGTCAGAAGCTCACGCGTGGCGGATGT NFKB1 NM_003998 12 4 + 35 − 0 37 1 0 CGCGAATGCCCTACCACCGCCGAAACTATCCGA 2054 AAAATTGGGCATACGCGTGGCGGATGT NFKB1 NM_003998 12 4 + 70 + 0 37 1 0 CGCGAATGCCTGGTGCTGGAGCTGGAGGCGGAG 2055 GCATGTTTGGTAACGCGTGGCGGATGT NFKB1 NM_003998 12 4 + 105 − 0 37 1 0 CGCGAATGCCACCTGTACTTCCAGTGCCCCCTCC 2056 TCCACCGCCACACGCGTGGCGGATGT NFKB1 NM_003998 12 4 + 140 + 0 37 1 0 CGCGAATGCCCCAGGTACAAAAATACTTATTCT 2057 TCCTAAAACTTTACGCGTGGCGGATGT NFKB1 NM_003998 21 4 + −36 + 0 37 1 0 CGCGAATGCCATGTATAACGATTTCTGGTGTTTT 2058 TCTTTCCAACAACGCGTGGCGGATGT NFKB1 NM_003998 21 4 + −1 − 0 37 1 0 CGCGAATGCCGCTCATATGGTTTCCCATTTAATA 2059 TGTCAAATACCACGCGTGGCGGATGT NFKB1 NM_003998 21 4 + 34 + 0 37 1 0 CGCGAATGCCCAGAGTTTACATCTGATGATTTA 2060 CTAGCACAAGGTACGCGTGGCGGATGT NFKB1 NM_003998 21 4 + 69 − 0 37 1 0 CGCGAATGCCATAATGGTTAAGAGAATCTGGTT 2061 TTATCACAACCCACGCGTGGCGGATGT EPHA4 NM_004438 7 2 − −39 + 0 37 1 0 CGCGAATGCCCTCATCAAAGGTTTGTGTTTTTCT 2062 TTCTGAAAACTACGCGTGGCGGATGT EPHA4 NM_004438 7 2 − −4 − 0 37 1 0 CGCGAATGCCATGTACTCTGTTATGATCATTACT 2063 GGTTTACCTAGACGCGTGGCGGATGT EPHA4 NM_004438 7 2 − 31 + 0 37 1 0 CGCGAATGCCGGAGAATGGCTCCTTGGATGCAT 2064 TCCTCAGGGTATACGCGTGGCGGATGT EPHA4 NM_004438 7 2 − 66 − 0 37 1 0 CGCGAATGCCTACCAACATTCTTGGGTTTAATAT 2065 AAAGTAGTCACACGCGTGGCGGATGT RET NM_020630 17 10 + −1 + 0 37 1 0 CGCGAATGCCGATGGTCTTTTGGTGTCCTGCTGT 2066 GGGAGATCGTGACGCGTGGCGGATGT RET NM_020630 17 10 + 34 − 0 37 1 0 CGCGAATGCCGGAGGAATCCCAGGATAGGGGTT 2067 TCCCCCTAGGGTACGCGTGGCGGATGT RET NM_020630 17 10 + 69 + 0 37 1 0 CGCGAATGCCTGAGCGGCTCTTCAACCTTCTGA 2068 AGACCGGCCACCACGCGTGGCGGATGT RET NM_020630 17 10 + 104 − 0 37 1 0 CGCGAATGCCCATCTCCTCGCTGCAGTTGTCTGG 2069 CCTCTCCATCCACGCGTGGCGGATGT EPHA4 NM_004438 12 2 − 0 + 0 37 1 0 CGCGAATGCCGATCAGAATGAGCGAAGCTATCG 2070 TATAGTTCGGACACGCGTGGCGGATGT EPHA4 NM_004438 12 2 − 35 − 0 37 1 0 CGCGAATGCCGGTTCAGGCCTTTGATATCTGTGT 2071 TCCTGGCAGCTACGCGTGGCGGATGT EPHA4 NM_004438 12 2 − 70 + 0 37 1 0 CGCGAATGCCCTCTCACTTCCTATGTTTTCCACG 2072 TGCGAGCCAGGACGCGTGGCGGATGT EPHA4 NM_004438 12 2 − 105 − 0 37 1 0 CGCGAATGCCAAGGGCTCACTGAAGTCTCCATA 2073 GCCAGCTGCTGTACGCGTGGCGGATGT EPHA4 NM_004438 12 2 − 140 + 0 37 1 0 CGCGAATGCCGGAGGTTACAACCAACACAGGTA 2074 ACAAGGACCACCACGCGTGGCGGATGT KSR2 NM_173598 1 12 − −66 + 0 37 1 0 CGCGAATGCCACAACGGATGACATCATCATGTC 2075 CTTGTGACCTCTACGCGTGGCGGATGT KSR2 NM_173598 1 12 − −31 − 0 37 1 0 CGCGAATGCCCAGCCTGGAGTGGGGAGAGAAG 2076 GGAGAGAGTGGTGACGCGTGGCGGATGT KSR2 NM_173598 1 12 − 4 + 0 37 1 0 CGCGAATGCCTGACCTTTGGACATCGGGACGGC 2077 GCCCAGCTGCCTACGCGTGGCGGATGT KSR2 NM_173598 1 12 − 39 − 0 37 1 0 CGCGAATGCCAGGACAGAGTAGGGAGGGAGAG 2078 GTGACGGGAGCCCACGCGTGGCGGATGT PALB2 NM_024675 7 16 − 0 + 0 37 1 0 CGCGAATGCCAATCCTTCAGGTTCCTGTTCCGTA 2079 GATGTGAGTGCACGCGTGGCGGATGT PALB2 NM_024675 7 16 − 35 − 0 37 1 0 CGCGAATGCCATGGCTCTTTACAACCGGCTCTTT 2080 CCCAAAACATGACGCGTGGCGGATGT PALB2 NM_024675 7 16 − 70 + 0 37 1 0 CGCGAATGCCGTATCATAACTGCTTGCGAAGAT 2081 GTAGTTTCTCTTACGCGTGGCGGATGT PALB2 NM_024675 7 16 − 105 − 0 37 1 0 CGCGAATGCCAGTTTTTCCCACTGCCAAGCATCC 2082 AGAGCTTTCCAACGCGTGGCGGATGT PALB2 NM_024675 7 16 − 140 + 0 37 1 0 CGCGAATGCCTTATACCTGGCACTTCGCAGAGG 2083 TAAGTGGGAATCACGCGTGGCGGATGT PIK3CA NM_006218 18 3 + 0 + 0 37 1 0 CGCGAATGCCAATGTTACCTTATGGTTGTCTGTC 2084 AATCGGTGACTACGCGTGGCGGATGT PIK3CA NM_006218 18 3 + 35 − 0 37 1 0 CGCGAATGCCGTGAGAATTTCGCACCACCTCAA 2085 TAAGTCCCACACACGCGTGGCGGATGT PIK3CA NM_006218 18 3 + 70 + 0 37 1 0 CGCGAATGCCACTATTATGCAAATTCAGTGCAA 2086 AGGCGGCTTGAAACGCGTGGCGGATGT PIK3CA NM_006218 18 3 + 105 − 0 37 1 0 CGCGAATGCCGATGTAGTGTGTGGCTGTTGAAC 2087 TGCAGTGCACCTACGCGTGGCGGATGT PIK3CA NM_006218 18 3 + 140 + 0 37 1 0 CGCGAATGCCAGTGGCTCAAAGACAAGAACAA 2088 AGGAGAAATGTGAACGCGTGGCGGATGT CENTG1 NM_001122772 6 12 − −36 + 24 37 1 0 CGCGAATGCCCATTCTATCTCTATCTCCTTCGCT 2089 TCGGGAAACCAACGCGTGGCGGATGT CENTG1 NM_001122772 6 12 − −5 − 0 37 1 0 CGCGAATGCCAGGATTTTAGTTTCCACATTTTGC 2090 GCTTGGCTGGTACGCGTGGCGGATGT CENTG1 NM_001122772 6 12 − 30 + 0 37 1 0 CGCGAATGCCTTGGTAGTTTAAGAAATATTTAT 2091 AAAGCAGGTAACACGCGTGGCGGATGT CENTG1 NM_001122772 6 12 − 65 − 0 37 1 0 CGCGAATGCCCACCCCAGCTGAGCGCCCCTCCC 2092 GGCTCCCCACTTACGCGTGGCGGATGT EPHA7 NM_004440 12 6 − −8 + 0 37 1 0 CGCGAATGCCCCAAACAGCTCCCTCGCAAGTGA 2093 GTGGAGTAATGAACGCGTGGCGGATGT EPHA7 NM_004440 12 6 − 27 − 0 37 1 0 CGCGAATGCCGGAAAGCTCGACACTCCGCTGCA 2094 GTACTCTCTCCTACGCGTGGCGGATGT EPHA7 NM_004440 12 6 − 62 + 0 37 1 0 CGCGAATGCCTGGCAGGAACCAGAGCATCCCAA 2095 TGGAGTCATCACACGCGTGGCGGATGT EPHA7 NM_004440 12 6 − 97 − 0 37 1 0 CGCGAATGCCCACTTACTTTCTCGTAATACTTGA 2096 TTTCATATTCTACGCGTGGCGGATGT PDGFRA NM_006206 15 4 + 0 + 0 37 1 0 CGCGAATGCCGCCCCATTTACATCATCACAGAG 2097 TATTGCTTCTATACGCGTGGCGGATGT PDGFRA NM_006206 15 4 + 35 − 0 37 1 0 CGCGAATGCCTCCCTATTCTTATGCAAATAGTTG 2098 ACCAAATCTCCACGCGTGGCGGATGT PDGFRA NM_006206 15 4 + 70 + 0 37 1 0 CGCGAATGCCTAGCTTCCTGAGCCACCACCCAG 2099 AGAAGCCAAAGAACGCGTGGCGGATGT PDGFRA NM_006206 15 4 + 105 − 0 37 1 0 CGCGAATGCCATCAGCAGGGTTCAATCCAAAGA 2100 TATCCAGCTCTTACGCGTGGCGGATGT PDGFRA NM_006206 15 4 + 140 + 0 37 1 0 CGCGAATGCCGAAAGCACACGGAGGTGGGTGC 2101 AAAGAGAGATGTTACGCGTGGCGGATGT RET NM_020630 8 10 + −7 + 0 37 1 0 CGCGAATGCCCCTGCAGATGTGGCCGAGGAGGC 2102 GGGCTGCCCCCTACGCGTGGCGGATGT RET NM_020630 8 10 + 28 − 0 37 1 0 CGCGAATGCCCACACTCCAGCCGTCTCTTGCTG 2103 ACTGCACAGGACACGCGTGGCGGATGT RET NM_020630 8 10 + 63 + 0 37 1 0 CGCGAATGCCAGGAGTGTGGCGGCCTGGGCTCC 2104 CCAACAGGCAGGACGCGTGGCGGATGT RET NM_020630 8 10 + 98 − 0 37 1 0 CGCGAATGCCGGCTTACCTTTGCCATCTCCTTGC 2105 CTCCACTCACAACGCGTGGCGGATGT PDGFRA NM_006206 4 4 + 0 + 0 37 1 0 CGCGAATGCCACCCAGATGTAGCCTTTGTACCT 2106 CTAGGAATGACGACGCGTGGCGGATGT PDGFRA NM_006206 4 4 + 35 − 0 37 1 0 CGCGAATGCCGCAGAATCATCATCCTCCACGAT 2107 GACTAAATAATCACGCGTGGCGGATGT PDGFRA NM_006206 4 4 + 70 + 0 37 1 0 CGCGAATGCCCATTATACCTTGTCGCACAACTG 2108 ATCCCGAGACTCACGCGTGGCGGATGT PDGFRA NM_006206 4 4 + 105 − 0 37 1 0 CGCGAATGCCAGGTACCACCCCCTCACTGTTGT 2109 GTAAGGTTACAGACGCGTGGCGGATGT PDGFRA NM_006206 4 4 + 140 + 0 37 1 0 CGCGAATGCCGCCTCCTACGACAGCAGACAGGG 2110 CTTTAATGGGACACGCGTGGCGGATGT PDGFRA NM_006206 4 4 + 175 − 0 37 1 0 CGCGAATGCCCGGTGGCCTCACAGATATAGGGC 2111 CCTACAGTGAAGACGCGTGGCGGATGT PDGFRA NM_006206 4 4 + 210 + 0 37 1 0 CGCGAATGCCTCAAAGGAAAGAAGTTCCAGACC 2112 ATCCCATTTAATACGCGTGGCGGATGT PDGFRA NM_006206 4 4 + 245 − 0 37 1 0 CGCGAATGCCAAGGAGATGATACAAGTACCTTT 2113 TAAAGCATAAACACGCGTGGCGGATGT RET NM_020630 15 10 + −8 + 0 37 1 0 CGCGAATGCCCCTCACAGCTCGTTCATCGGGAC 2114 TTGGCAGCCAGAACGCGTGGCGGATGT RET NM_020630 15 10 + 27 − 0 37 1 0 CGCGAATGCCATCTTCATCTTCCGCCCCTCAGCT 2115 ACCAGGATGTTACGCGTGGCGGATGT RET NM_020630 15 10 + 62 + 0 37 1 0 CGCGAATGCCTTCGGATTTCGGCTTGTCCCGAG 2116 ATGTTTATGAAGACGCGTGGCGGATGT RET NM_020630 15 10 + 97 − 0 37 1 0 CGCGAATGCCACTGGGCACCTGGCTCCTCTTCA 2117 CGTAGGAATCCTACGCGTGGCGGATGT RPS6KA1 NM_002953 19 1 + −32 + 0 37 1 0 CGCGAATGCCCAGACTGACCACCTCCCCTGCCC 2118 TGTTGCCAGGTGACGCGTGGCGGATGT RPS6KA1 NM_002953 19 1 + 3 − 0 37 1 0 CGCGAATGCCATGTCGCAGCCTTCATCGTAGCC 2119 CTGGCGCTTCAGACGCGTGGCGGATGT RPS6KA1 NM_002953 19 1 + 38 + 0 37 1 0 CGCGAATGCCCTGGAGCCTGGGCATTCTGCTGT 2120 ACACCATGCTGGACGCGTGGCGGATGT RPS6KA1 NM_002953 19 1 + 73 − 0 37 1 0 CGCGAATGCCGTGGGGAAGGGTCCAGGCCAGG 2121 GGCACTCACCCTGACGCGTGGCGGATGT RB1 NM_000321 16 13 + −2 + 0 37 1 0 CGCGAATGCCAGGAAGAAGAACGATTATCCATT 2122 CAAAATTTTAGGACGCGTGGCGGATGT RB1 NM_000321 16 13 + 31 − 33 37 1 0 CGCGAATGCCGAAAAAAATTTTTTACTAAAAGT 2123 AAAAAATTTACCACGCGTGGCGGATGT RB1 NM_000321 16 13 + 74 + 31 37 1 0 CGCGAATGCCGAAGTAAGTATTTTATAATCTTTT 2124 TTTTTTTCCTTACGCGTGGCGGATGT RB1 NM_000321 16 13 + 105 − 0 37 1 0 CGCGAATGCCATGAAAAATGTTGTCATTCAGAA 2125 GTTTGCTAAAGGACGCGTGGCGGATGT RB1 NM_000321 16 13 + 140 + 0 37 1 0 CGCGAATGCCATGTCTTTATTGGCGTGCGCTCTT 2126 GAGGTTGTAATACGCGTGGCGGATGT RB1 NM_000321 16 13 + 175 − 0 37 1 0 CGCGAATGCCATTTATGAAAATTTAACTTACTGC 2127 TATATGTGGCCACGCGTGGCGGATGT RPS6KA1 NM_002953 7 1 + −16 + 0 37 1 0 CGCGAATGCCCTCCTGTCTTTTGCAGGTGATGTT 2128 CACGGAGGAGGACGCGTGGCGGATGT RPS6KA1 NM_002953 7 1 + 19 − 0 37 1 0 CGCGAATGCCGCCCAGAGCCAGCTCAGCCAGGT 2129 AAAACTTCACATACGCGTGGCGGATGT RPS6KA1 NM_002953 7 1 + 54 + 0 37 1 0 CGCGAATGCCCTGGATCACCTGCACAGCCTGGG 2130 TATCATTTACAGACGCGTGGCGGATGT RPS6KA1 NM_002953 7 1 + 89 − 0 37 1 0 CGCGAATGCCCTGGAGGCTTCACTCACTTCTCA 2131 GGCTTGAGGTCTACGCGTGGCGGATGT PKN1 NM_002741 3 19 + 0 + 0 37 1 0 CGCGAATGCCATGGCCCCCAGTCCCCTGGTGCG 2132 GGTGGCCCCACCACGCGTGGCGGATGT PKN1 NM_002741 3 19 + 35 − 0 37 1 0 CGCGAATGCCAGGCCCGCCACGCGGCTCAGGTT 2133 GGTGGCCGAGCAACGCGTGGCGGATGT PKN1 NM_002741 3 19 + 70 + 0 37 1 0 CGCGAATGCCGGAGAAGCAGTTGGCCATTGAGC 2134 TGAAGGTGAAGCACGCGTGGCGGATGT PKN1 NM_002741 3 19 + 105 − 0 37 1 0 CGCGAATGCCATTGCTGTAGGTCTGGATCATGTT 2135 CTCCGCCCCCTACGCGTGGCGGATGT PKN1 NM_002741 3 19 + 140 + 0 37 1 0 CGCGAATGCCGGCAGCACCAAGGTGAGGCAGC 2136 ACGTGCACACACAACGCGTGGCGGATGT PDGFRA NM_006206 16 4 + 0 + 0 37 1 0 CGCGAATGCCCTATGTTATTTTATCTTTTGAAAA 2137 CAATGGTGACTACGCGTGGCGGATGT PDGFRA NM_006206 16 4 + 35 − 0 37 1 0 CGCGAATGCCATACTGTGTAGTATCAGCCTGCTT 2138 CATGTCCATGTACGCGTGGCGGATGT PDGFRA NM_006206 16 4 + 70 + 0 37 1 0 CGCGAATGCCGTCCCCATGCTAGAAAGGAAAGA 2139 GGTTTCTAAATAACGCGTGGCGGATGT PDGFRA NM_006206 16 4 + 105 − 0 37 1 0 CGCGAATGCCCTGGACGATCATAGAGTGATCTC 2140 TGGATGTCGGAAACGCGTGGCGGATGT PDGFRA NM_006206 16 4 + 140 + 0 37 1 0 CGCGAATGCCCCTCATATAAGAAGAAATCTATG 2141 TTAGGTAAAAGTACGCGTGGCGGATGT RB1 NM_000321 25 13 + 0 + 0 37 1 0 CGCGAATGCCACTTCTGAGAAGTTCCAGAAAAT 2142 AAATCAGATGGTACGCGTGGCGGATGT RB1 NM_000321 25 13 + 35 − 0 37 1 0 CGCGAATGCCCAGCACTTCTTTTGAGCACACGG 2143 TCGCTGTTACATACGCGTGGCGGATGT RB1 NM_000321 25 13 + 70 + 0 37 1 0 CGCGAATGCCAAGGAAGCAACCCTCCTAAACCA 2144 CTGAAAAAACTAACGCGTGGCGGATGT RB1 NM_000321 25 13 + 105 − 0 37 1 0 CGCGAATGCCCCATCTGCTTCATCTGATCCTTCA 2145 ATATCAAAGCGACGCGTGGCGGATGT RB1 NM_000321 25 13 + 140 + 0 37 1 0 CGCGAATGCCAAGGTAGGAACCAGTTTTGAATG 2146 TTTTCCAGTAGCACGCGTGGCGGATGT PKN1 NM_213560 1 19 + −50 + 0 37 1 0 CGCGAATGCCGGTGGGGCTGAGGTTCAGGAAGA 2147 GGGCGGGGCCCTACGCGTGGCGGATGT PKN1 NM_213560 1 19 + −15 − 0 37 1 0 CGCGAATGCCGGGTTATTGGCCTCCGCCATCCT 2148 GGGTCCGGGCTGACGCGTGGCGGATGT PKN1 NM_213560 1 19 + 20 + 0 37 1 0 CGCGAATGCCCTCGGAGCAGGAGCTGGAGGTGG 2149 GGTCCAGGGTCCACGCGTGGCGGATGT PKN1 NM_213560 1 19 + 55 − 0 37 1 0 CGCGAATGCCTGCCACTTGGGGCCCCCTTCTGCC 2150 TGCCCCCACAGACGCGTGGCGGATGT PDGFRA NM_006206 10 4 + 0 + 0 37 1 0 CGCGAATGCCATGTAATAATGAAACTTCCTGGA 2151 CTATTTTGGCCAACGCGTGGCGGATGT PDGFRA NM_006206 10 4 + 35 − 0 37 1 0 CGCGAATGCCGGAGTGGATCTCCGTGATGATGT 2152 TTGAGACATTGTACGCGTGGCGGATGT PDGFRA NM_006206 10 4 + 70 + 0 37 1 0 CGCGAATGCCCGAGACAGGAGTACCGTGGAGG 2153 GCCGTGTGACTTTACGCGTGGCGGATGT PDGFRA NM_006206 10 4 + 105 − 0 37 1 0 CGCGAATGCCGGCATCGCACGGCGATGGTCTCC 2154 TCCACTTTGGCGACGCGTGGCGGATGT PDGFRA NM_006206 10 4 + 140 + 0 37 1 0 CGCGAATGCCTGGCTAAGAATCTCCTTGGAGCT 2155 GAGAACCGAGAGACGCGTGGCGGATGT PDGFRA NM_006206 10 4 + 175 − 0 37 1 0 CGCGAATGCCCTGTTGAGGAACTCACTGGGAGC 2156 CACCAGCTTCAGACGCGTGGCGGATGT NTRK3 NM_001012338 14 15 − 0 + 0 37 1 0 CGCGAATGCCGCAGTTGGAGCAGAACTTTTTCA 2157 ACTGCAGCTGTGACGCGTGGCGGATGT NTRK3 NM_001012338 14 15 − 35 − 0 37 1 0 CGCGAATGCCCCCCTGCTCCTGCCAGAGCTGCA 2158 TCCAGCGGATGTACGCGTGGCGGATGT NTRK3 NM_001012338 14 15 − 70 + 0 37 1 0 CGCGAATGCCGAGGCCAAGCTCAACAGCCAGA 2159 ACCTCTACTGCATACGCGTGGCGGATGT NTRK3 NM_001012338 14 15 − 105 − 0 37 1 0 CGCGAATGCCTGCGGAAGAGAGGAAGCTGGGA 2160 GCCATCAGCGTTGACGCGTGGCGGATGT NTRK3 NM_001012338 14 15 − 140 + 0 37 1 0 CGCGAATGCCTGAACATCAGTCAGTGTGGTGAG 2161 TGAGTGGCCGCCACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 0 + 0 37 1 0 CGCGAATGCCCCCGTCTGCCGTTTAAGCGCCTG 2162 AATCTTGTCCCAACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 35 − 0 37 1 0 CGCGAATGCCCCCTGATCGTCTGACATGTCATC 2163 GGCTTTCCCCTTACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 70 + 0 37 1 0 CGCGAATGCCTACTTCTGTGCAAAGTAAAAGCC 2164 CCGATTTAGAGGACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 105 − 0 37 1 0 CGCGAATGCCCACATGACAGTTGTTTTCCAAGG 2165 TGTCCAAAGAGGACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 140 + 0 37 1 0 CGCGAATGCCGGTTCTGACATAGACTTTAGACC 2166 GAAACTTGTCAAACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 175 − 0 37 1 0 CGCGAATGCCTATTTCTTAAAAAGTTATCTAAG 2167 GGACCCTTCCCGACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 210 + 0 37 1 0 CGCGAATGCCGAATCGAAACCAGTATTGGCCAG 2168 AGCACAGTCATCACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 245 − 0 37 1 0 CGCGAATGCCTCTGGCTGCTCATTCGAGTCCTCT 2169 GTCAAATCAATACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 280 + 0 37 1 0 CGCGAATGCCCAGTCTTGTGGACCACAATAAAC 2170 TAAATTCTGAAGACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 315 − 0 37 1 0 CGCGAATGCCTCGCTGGCCATTTATTGCCTCCCT 2171 GGAGGGAGAGGACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 350 + 0 37 1 0 CGCGAATGCCGAAGACACTGGGGATCAGCAGG 2172 GGTTGTTGAAGGCACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 385 − 0 37 1 0 CGCGAATGCCTCTCTCCAGGAAATGCCAACTTG 2173 TCGTTCTGAATGACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 420 + 0 37 1 0 CGCGAATGCCCCCTTTCAGACATTCCTTGCAAA 2174 ACAGAGGAGGAGACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 455 − 0 37 1 0 CGCGAATGCCTCGCCTCTCCTCCCTGCACCTCCA 2175 CAGCCAACACCACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 490 + 0 37 1 0 CGCGAATGCCCTCCCAGGAATGTTCGCCACGGA 2176 GCTGCCCGGAGCACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 525 − 0 37 1 0 CGCGAATGCCCTCCTTTCTGGGGCACATTCTCGG 2177 GCCACTCGTCAACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 560 + 0 37 1 0 CGCGAATGCCCAGGACAGTTGGAGTGAAGCTGG 2178 GGGCATCCTGTTACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 595 − 0 37 1 0 CGCGAATGCCTGTCCTGCAAGACCACCATAGGC 2179 ACCTTCCCTTTGACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 630 + 0 37 1 0 CGCGAATGCCTCTTGGCTGTGAGACCACCGCAA 2180 ATCAAGTCCCTTACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 665 − 0 37 1 0 CGCGAATGCCTCAGGGGTCATGTTCTTGCCTTGG 2181 GGTGTGGCTGGACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 700 + 0 37 1 0 CGCGAATGCCGAGTGAGGTGCTGGAATCTTTCC 2182 CCGAAGAAGACTACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 735 − 0 37 1 0 CGCGAATGCCAGAGGGAGAGCTCAGGGACGAA 2183 TGGCTGAGTACAGACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 770 + 0 37 1 0 CGCGAATGCCTCCACCAGCTCGCCCGAGGGGCC 2184 GCCTGCTCCCCCACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 805 − 0 37 1 0 CGCGAATGCCAGGTGGGGAAGGGACTGGTACTG 2185 CTGTGCTGCTTTACGCGTGGCGGATGT CHAF1A NM_005483 3 19 + 840 + 0 37 1 0 CGCGAATGCCCCACGCCCCTCCGCAGAGTGAGT 2186 ATCTCCCATGGAACGCGTGGCGGATGT EPHA4 NM_004438 18 2 − −24 + 0 37 1 0 CGCGAATGCCGAGCAGCGTTGGCACCGGCGAAC 2187 CATGGCTGGGATACGCGTGGCGGATGT EPHA4 NM_004438 18 2 − 11 − 0 37 1 0 CGCGAATGCCTCCCGAAGAGACACGAAAATAG 2188 GGCGAAATAGAAAACGCGTGGCGGATGT EPHA4 NM_004438 18 2 − 46 + 0 37 1 0 CGCGAATGCCTTTGCGACGCTGTCACAGGTTCC 2189 AGGGTATACCCCACGCGTGGCGGATGT EPHA4 NM_004438 18 2 − 81 − 0 37 1 0 CGCGAATGCCCACAGAAAGGCCGTCCCGCTCTT 2190 ACCTTCATTCGCACGCGTGGCGGATGT GUCY2F NM_001522 18 X − 0 + 0 37 1 0 CGCGAATGCCTAATCATCATGTGTATGCATTCA 2191 GCTTTGATTGGGACGCGTGGCGGATGT GUCY2F NM_001522 18 X − 35 − 0 37 1 0 CGCGAATGCCTGAGCACATTCCAAGAGATGCAT 2192 CTGAGTCTCTCCACGCGTGGCGGATGT GUCY2F NM_001522 18 X − 70 + 0 37 1 0 CGCGAATGCCTGATCTGAAAATGACTGATGGAA 2193 CCTACGTCTTTGACGCGTGGCGGATGT GUCY2F NM_001522 18 X − 105 − 0 37 1 0 CGCGAATGCCATAAGGTAAACTGTAGAGCAGGG 2194 CATCATAAGGAAACGCGTGGCGGATGT GUCY2F NM_001522 18 X − 140 + 0 37 1 0 CGCGAATGCCAAGCACACCCCCTACCGGGTCCT 2195 AAGGAACAACCCACGCGTGGCGGATGT GUCY2F NM_001522 18 X − 175 − 0 37 1 0 CGCGAATGCCTGGTCAACACTGCATCATAGGCT 2196 TCCCGGAGCTTTACGCGTGGCGGATGT GUCY2F NM_001522 18 X − 210 + 0 37 1 0 CGCGAATGCCTTACAGTGGAGTCCCAAGAAAAG 2197 ACCTTCTATCAAACGCGTGGCGGATGT GUCY2F NM_001522 18 X − 245 − 0 37 1 0 CGCGAATGCCGGAATTTCACCTCTTGCTGCTGCC 2198 TCTGTGAAGGCACGCGTGGCGGATGT GUCY2F NM_001522 18 X − 280 + 0 37 1 0 CGCGAATGCCTGAGAAGCTGGAGTTCGATCAAG 2199 TAAGTACACATTACGCGTGGCGGATGT CHAF1A NM_005483 11 19 + −24 + 0 37 1 0 CGCGAATGCCCTCTTTTTTTTTTCTCCTTTTGAGG 2200 ATGATGATGAACGCGTGGCGGATGT CHAF1A NM_005483 11 19 + 11 − 0 37 1 0 CGCGAATGCCAACCATCGTCCTCATCTTCATCCT 2201 CTCCCATGTCGACGCGTGGCGGATGT CHAF1A NM_005483 11 19 + 46 + 0 37 1 0 CGCGAATGCCTCTTTGTGCCCCATGGGTACCTGT 2202 CTGAGGACGAAACGCGTGGCGGATGT CHAF1A NM_005483 11 19 + 81 − 0 37 1 0 CGCGAATGCCGACCTCCCCCTTCACTCCCTCACC 2203 TCTGTCACACCACGCGTGGCGGATGT RET NM_020630 6 10 + 0 + 0 37 1 0 CGCGAATGCCGGCTGGTTCTCAACCGGAACCTC 2204 TCCATCTCGGAGACGCGTGGCGGATGT RET NM_020630 6 10 + 35 − 0 37 1 0 CGCGAATGCCTCATTGACCAGCACCGCCAGCTG 2205 CATGGTGCGGTTACGCGTGGCGGATGT RET NM_020630 6 10 + 70 + 0 37 1 0 CGCGAATGCCCTCAGACTTCCAGGGCCCAGGAG 2206 CGGGCGTCCTCTACGCGTGGCGGATGT RET NM_020630 6 10 + 105 − 0 37 1 0 CGCGAATGCCGCTGACCGGCAGCACCGACACGT 2207 TGAAGTGGAGCAACGCGTGGCGGATGT RET NM_020630 6 10 + 140 + 0 37 1 0 CGCGAATGCCCTGCACCTGCCCAGTACCTACTC 2208 CCTCTCCGTGAGACGCGTGGCGGATGT RET NM_020630 6 10 + 175 − 0 37 1 0 CGCGAATGCCATGGGCTCACCTGGGCAAATCGG 2209 CGAGCCCTCCTGACGCGTGGCGGATGT NTRK3 NM_001012338 9 15 − −38 + 0 37 1 0 CGCGAATGCCAGCTAACTGTCCTCCTCCTTTTTG 2210 TGTTTGGTTTTACGCGTGGCGGATGT NTRK3 NM_001012338 9 15 − −3 − 0 37 1 0 CGCGAATGCCAGTGATAGGAGGTGTGGGACTCA 2211 CTTCGTCAACTGACGCGTGGCGGATGT NTRK3 NM_001012338 9 15 − 32 + 0 37 1 0 CGCGAATGCCGTGACCCACAAACCAGAAGAAG 2212 ACACTTTTGGGGTACGCGTGGCGGATGT NTRK3 NM_001012338 9 15 − 67 − 0 37 1 0 CGCGAATGCCCAGTCAACACACTCCTCTTGACC 2213 AAGAAGTGACTCACGCGTGGCGGATGT PDGFRA NM_006206 5 4 + −4 + 0 37 1 0 CGCGAATGCCATAGCAACATCAGAGCTGGATCT 2214 AGAAATGGAAGCACGCGTGGCGGATGT PDGFRA NM_006206 5 4 + 31 − 0 37 1 0 CGCGAATGCCCAATCGTTTCCCCTGACTTATACA 2215 CGGTTTTAAGAACGCGTGGCGGATGT PDGFRA NM_006206 5 4 + 66 + 0 37 1 0 CGCGAATGCCTGGTCACCTGTGCTGTTTTTAACA 2216 ATGAGGTGGTTACGCGTGGCGGATGT PDGFRA NM_006206 5 4 + 101 − 0 37 1 0 CGCGAATGCCCCTACCACTTCTCCAGGGTAAGT 2217 CCATTGAAGGTCACGCGTGGCGGATGT RET NM_020630 13 10 + −16 + 0 37 1 0 CGCGAATGCCCTGTGCTGCATTTCAGAGAACGC 2218 CTCCCCGAGTGAACGCGTGGCGGATGT RET NM_020630 13 10 + 19 − 0 37 1 0 CGCGAATGCCTCAGGACGTTGAACTCTGACAGC 2219 AGGTCTCGCAGCACGCGTGGCGGATGT RET NM_020630 13 10 + 54 + 0 37 1 0 CGCGAATGCCAGCAGGTCAACCACCCACATGTC 2220 ATCAAATTGTATACGCGTGGCGGATGT RET NM_020630 13 10 + 89 − 0 37 1 0 CGCGAATGCCCTGCAGCTGGCCTTACCATCCTG 2221 GCTGCAGGCCCCACGCGTGGCGGATGT PALB2 NM_024675 3 16 − −26 + 0 37 1 0 CGCGAATGCCCAGCTTATTTATTTTTGTTATCTA 2222 AGGAATTTAAAACGCGTGGCGGATGT PALB2 NM_024675 3 16 − 9 − 0 37 1 0 CGCGAATGCCCATCAATGTGCATCTTTTTCAGGA 2223 GTTGACCAGTTACGCGTGGCGGATGT PALB2 NM_024675 3 16 − 44 + 0 37 1 0 CGCGAATGCCATTCTTACCAAGCTTCAGTCTGTC 2224 ACAAAGCCTATACGCGTGGCGGATGT PALB2 NM_024675 3 16 − 79 − 0 37 1 0 CGCGAATGCCAGTGGTCCCAGCCAGTCATTACT 2225 TACCATTTCAGAACGCGTGGCGGATGT EPHA4 NM_004438 5 2 − 0 + 0 37 1 0 CGCGAATGCCGGTGGCAAGATTCCTATCCGGTG 2226 GACTGCGCCAGAACGCGTGGCGGATGT EPHA4 NM_004438 5 2 − 35 − 0 37 1 0 CGCGAATGCCCACTTGCTGATGTGAATTTACGA 2227 TAGGCAATTGCTACGCGTGGCGGATGT EPHA4 NM_004438 5 2 − 70 + 0 37 1 0 CGCGAATGCCATGTATGGAGCTATGGAATCGTT 2228 ATGTGGGAAGTGACGCGTGGCGGATGT EPHA4 NM_004438 5 2 − 105 − 0 37 1 0 CGCGAATGCCGACATATCCCAATAGGGCCTCTC 2229 CCCGTACGACATACGCGTGGCGGATGT EPHA4 NM_004438 5 2 − 140 + 0 37 1 0 CGCGAATGCCCAATCAAGATGTAAGTCTCTATG 2230 TTCTGAAATATAACGCGTGGCGGATGT NTRK3 NM_001012338 13 15 − 0 + 0 37 1 0 CGCGAATGCCACCTTCCTGAGATCAGCGTGAGC 2231 CACGTCAACCTGACGCGTGGCGGATGT NTRK3 NM_001012338 13 15 − 35 − 0 37 1 0 CGCGAATGCCCAAGTGATAACAGCGTTGTCACC 2232 CTCTCGTACGGTACGCGTGGCGGATGT NTRK3 NM_001012338 13 15 − 70 + 0 37 1 0 CGCGAATGCCCAATGGCTCTGGATCACCCCTTC 2233 CTGATGTGGACTACGCGTGGCGGATGT NTRK3 NM_001012338 13 15 − 105 − 0 37 1 0 CGCGAATGCCGTGAGTGTTGATGGACTGCAGCC 2234 CAGTGACTATCCACGCGTGGCGGATGT NTRK3 NM_001012338 13 15 − 140 + 0 37 1 0 CGCGAATGCCCAGGTAGGCATCCTGGGCTTCAG 2235 CCCCATCAGGAGACGCGTGGCGGATGT RET NM_020630 4 10 + 0 + 0 37 1 0 CGCGAATGCCGTGAGGGTCTGCCCTTCCGCTGC 2236 GCCCCGGACAGCACGCGTGGCGGATGT RET NM_020630 4 10 + 35 − 0 37 1 0 CGCGAATGCCTCGCGGTCCAGGGCCCAGCGCGT 2237 GCTCACCTCCAGACGCGTGGCGGATGT RET NM_020630 4 10 + 70 + 0 37 1 0 CGCGAATGCCGCAGCGGGAGAAGTACGAGCTG 2238 GTGGCCGTGTGCAACGCGTGGCGGATGT RET NM_020630 4 10 + 105 − 0 37 1 0 CGCGAATGCCCATCACCACCTCCTCGCGCGCGC 2239 CGGCGTGCACGGACGCGTGGCGGATGT RET NM_020630 4 10 + 140 + 0 37 1 0 CGCGAATGCCGTGCCCTTCCCGGTGACCGTGTA 2240 CGACGAGGACGAACGCGTGGCGGATGT RET NM_020630 4 10 + 175 − 0 37 1 0 CGCGAATGCCCGGTGTCGACGCCCGCGGGGAAG 2241 GTGGGCGCCGAGACGCGTGGCGGATGT RET NM_020630 4 10 + 210 + 0 37 1 0 CGCGAATGCCCCAGCGCCGTGGTGGAGTTCAAG 2242 CGGAAGGAGGTGACGCGTGGCGGATGT GUCY2F NM_001522 7 X − 0 + 0 37 1 0 CGCGAATGCCATCAGTTGCTGAATCTCTCAAAA 2243 AGGGCTGCACAGACGCGTGGCGGATGT GUCY2F NM_001522 7 X − 35 − 0 37 1 0 CGCGAATGCCGTACAAGGTGACCAAGTCAAAGC 2244 CCTCAGGTTCAAACGCGTGGCGGATGT GUCY2F NM_001522 7 X − 70 + 0 37 1 0 CGCGAATGCCTTCAGCGACATTGTGGGCTTCAC 2245 AACCATTTCAGCACGCGTGGCGGATGT GUCY2F NM_001522 7 X − 105 − 0 37 1 0 CGCGAATGCCTCAGAAGATCCACGACCTCAATG 2246 GGCTCACTCATGACGCGTGGCGGATGT GUCY2F NM_001522 7 X − 140 + 0 37 1 0 CGCGAATGCCATGACCTGTACACACTCTTTGAT 2247 GCAATAATTGGCACGCGTGGCGGATGT GUCY2F NM_001522 7 X − 175 − 0 37 1 0 CGCGAATGCCCCTGCTAATTAACCTACCTTGTAG 2248 ACATCATGACTACGCGTGGCGGATGT NTRK3 NM_001012338 5 15 − 0 + 0 37 1 0 CGCGAATGCCGCCCTGAAGGATCCCACCCTGGC 2249 TGCCCGGAAGGAACGCGTGGCGGATGT NTRK3 NM_001012338 5 15 − 35 − 0 37 1 0 CGCGAATGCCGCAGGTTGGTGAGCAGCTCGGCC 2250 TCCCTCTGGAAAACGCGTGGCGGATGT NTRK3 NM_001012338 5 15 − 70 + 0 37 1 0 CGCGAATGCCAGCATGAGCACATTGTCAAGTTC 2251 TATGGAGTGTGCACGCGTGGCGGATGT NTRK3 NM_001012338 5 15 − 105 − 0 37 1 0 CGCGAATGCCTATTCAAAGACCATGATGAGGGG 2252 GTCCCCATCGCCACGCGTGGCGGATGT NTRK3 NM_001012338 5 15 − 140 + 0 37 1 0 CGCGAATGCCCATGAAGCATGGAGACCTGAATA 2253 AGTTCCTCAGGTACGCGTGGCGGATGT

While the preferred embodiment of the invention has been illustrated and described, it will be appreciated that various changes can be made therein without departing from the spirit and scope of the invention. 

1. A method for generating a population of single-stranded nucleic acid probes, each probe comprising a predetermined nucleotide sequence, the method comprising: a) providing a starting population of linear double-stranded nucleic acid precursor molecules each precursor molecule having (i) a probe region having the predetermined sequence which is flanked at a 5′ and a 3′ end by a first and a second restriction enzyme recognition sequence for generating ligation substrates and for ligating a plurality of the double-stranded nucleic acid precursor molecules into head-to-tail concatemers (ii) the 5′ flanking region including the first restriction enzyme recognition sequence and (iii) the 3′ flanking region including the second restriction enzyme recognition sequence; b) contacting the 5′ and 3′ flanking regions of the linear double-stranded nucleic acid precursor molecules with the first and second restriction enzymes to cleave the first and second restriction enzyme recognition sequences so as to generate the ligation substrates; c) ligating the ligation substrates together so as to generate a plurality of random head-to-tail concatemers; d) amplifying the plurality of head-to-tail concatemers; e) contacting the amplified head-to-tail concatemers with the first and second restriction enzymes so as to release a plurality double-stranded monomer linear precursor molecules; and f) selectively removing one strand of the double-stranded monomer linear precursor molecules so as to generate a population of single-stranded nucleic acid probes, each probe comprising the predetermined nucleotide sequence.
 2. The method of claim 1, wherein the single-stranded nucleic acid probes further comprise a region which hybridizes to a capture nucleic acid molecule.
 3. The method of claim 1, wherein the members of the starting population of the linear double-stranded nucleic acid precursor molecules each comprise the same nucleotide sequence in the 5′ flanking region or each comprise the same nucleotide sequence in the 3′ flanking region.
 4. The method of claim 1, wherein the 3′ flanking region further comprises a third restriction enzyme recognition sequence.
 5. The method of claim 1, wherein the members of the starting population of the linear double-stranded nucleic acid precursor molecules each comprise the same predetermined sequences or different predetermined sequences.
 6. The method of claim 1, wherein the ligation substrates of step (b) comprise overhanging nucleic acid ends capable of annealing together.
 7. The method of claim 1, wherein the first or second restriction enzyme recognition sequence is cleaved by a type II restriction enzyme.
 8. The method of claim 1, wherein the first or second restriction enzyme recognition sequence is cleaved by a Bsm1 enzyme.
 9. The method of claim 1, wherein each predetermined nucleotide sequence in the population of linear double-stranded nucleic acid precursor molecules comprise a nucleotide sequence which is at least 95% identical to at least a portion of a sense or anti-sense strand of a target nucleic acid sequence.
 10. The method of claim 9, wherein the predetermined sequence hybridizes to one target sequence or hybridizes to different target sequences.
 11. The method of claim 9, wherein the predetermined sequences in the population of linear double-stranded nucleic acid precursor molecules hybridize to at least 10 different exon nucleotide sequences.
 12. The method of claim 9, wherein the predetermined sequences in the population of linear double-stranded nucleic acid precursor molecules hybridize to at least 1000 different exon nucleotide sequences.
 13. The method of claim 9, wherein the predetermined sequences hybridize to the target sequence at an interval of at least every 35 bases across the target sequence.
 14. The method of claim 9, wherein the predetermined sequences hybridize to the target sequence of interest at an interval of one base across the target sequence.
 15. The method of claim 1, wherein the probe region comprises 20-200 nucleotides.
 16. The method of claim 1, wherein the predetermined nucleotide sequence comprises 10-50 nucleotides.
 17. The method of claim 2, wherein the region of the single-stranded nucleic acid probe which hybridizes to the capture nucleic acid molecule comprises 10-50 nucleotides.
 18. The method of claim 1, wherein the amplifying according to step (d) comprises isothermal amplification.
 19. The method of claim 18, wherein the amplifying according to step (d) comprises random amplification primers.
 20. The method of claim 19, wherein the random amplification primers each comprise a random 7-mer oligonucleotide and two additional nitroindole residues at the 5′ end.
 21. The method of claim 19, wherein the random amplification primers each comprise a random 7-mer oligonucleotide and a phosphorothioate linkage to the 3′ end.
 22. The method of claim 1, wherein the selectively removing one strand from the double-stranded monomer linear precursor molecules comprises: a) contacting the released precursor molecules of step (e) with alkaline phosphatase; b) contacting the released precursor molecules of step (e) with a third restriction enzyme which cleaves the third restriction enzyme recognition sequence; and c) contacting the released precursor molecules of step (e) with an exonuclease so as to selectively degrade the one strand of the double-stranded monomer linear precursor molecules.
 23. The method of claim 22, wherein the exonuclease is lambda exonuclease.
 24. The method of claim 2, wherein the capture nucleic acid molecule further comprises a protein binding partner.
 25. The method of claim 24, wherein the protein binding partner is biotin.
 26. The method of claim 1, wherein each single-stranded nucleic acid probe comprises (i) the predetermined nucleotide sequence having a nucleotide sequence which is at least 95% identical to at least a portion of a sense or an anti-sense strand of a target nucleic acid sequence and (ii) a region which hybridizes to a capture nucleic acid molecule.
 27. A population of single-stranded nucleic acid probes generated by the method of claim
 1. 28. The method of claim 1, wherein the starting population of linear double-stranded nucleic acid precursor molecules is generated by steps comprising: a) providing a population of a first single-stranded nucleic acid molecule comprising the 5′ flanking region, the probe region which comprises the predetermined sequence, and the capture sequence; b) providing a population of a second single-stranded nucleic acid molecules comprising the sequence which is complementary to the capture sequence, and the 3′ flanking region; c) annealing the first and second populations of the single-stranded nucleic acid molecules to form a nucleic acid duplex having overhanging 5′ ends; and d) conducting a polymerase-dependent strand extension reaction on the overhanging 5′ ends so as to generate the population of double-stranded nucleic acid precursor molecules.
 29. A method for enriching a target nucleic acid sequence of interest from a nucleic acid library, comprising: a) contacting the population of single-stranded nucleic acid probes of claim 1 with the nucleic acid library having at least one target nucleic acid sequence of interest to form a mixture having unhybridized nucleic acid sequences and duplexes, each duplex having the single-stranded nucleic acid probe hybridized to the target nucleic acid sequence of interest; b) contacting the duplexes with a population of capture nucleic acid molecules to form complexes having the single-stranded nucleic acid probe hybridized to the target nucleic acid sequence of interest and hybridized to the capture nucleic acid molecule; c) separating the complex from the mixture; and d) eluting the target nucleic acid sequence of interest from the complex. 